Published online before print July 13, 2006, doi: 10.1161/01.RES.0000236754.21499.1c
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 31, 2006
Revised on June 20, 2006
Accepted on June 28, 2006
Akt Promotes Increased Cardiomyocyte Cycling and Expansion of the Cardiac Progenitor Cell Population
Natalie Gude ;
From the San Diego State University Heart Institute, Department of Biology (N.G., J.M., M.R., M.S.), San Diego State University; the New York Medical College, Cardiovascular Research Institute (J.K., P.A.), Valhalla, NY; and Biosource International (E.S.), Hopkinton, Mass.
* To whom correspondence should be addressed. E-mail: sussman{at}heart.sdsu.edu.
Activation of Akt is associated with enhanced cell cycling and cellular proliferation in nonmyocytes, but this effect of nuclear Akt accumulation has not been explored in the context of the myocardium. Cardiac-specific expression of nuclear-targeted Akt in transgenics prolongs postnatal cell cycling as evidenced by increased numbers of Ki67+ cardiomyocytes at 2 to 3 weeks after birth. Similarly, nuclear-targeting of Akt promotes expansion of the presumptive cardiac progenitor cell population as assessed by immunolabeling for c-kit in combination with myocyte-specific markers Nkx 2.5 or MEF 2C. Increases in pro-proliferative cytokines, including tumor-necrosis superfamily 8, interleukin-17e, and hepatocyte growth factor, were found in nuclear-targeted Akt myocardial samples. Concurrent signaling mediated by paracrine factors downstream of Akt/nuc expression may be responsible for phenotypic effects of nuclear-targeted Akt in the myocardium, including enhanced cell proliferation and expansion of the stem cell population.
Key words: PKB/Akt • cardiac stem cell • proliferation • cytokines • postnatal growth
Related Article:
- Effects of Akt on Cardiac Myocytes: Location Counts
- Daniele Catalucci and Gianluigi Condorelli
Circ. Res. 2006 99: 339-341.[Extract] [Full Text] [PDF]
This article has been cited by other articles:
 |
|
 |
|
  D. P. Del Re and J. Sadoshima Optimizing Cell-Based Therapy for Cardiac Regeneration Circulation, September 8, 2009; 120(10): 831 - 834. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Stamm, Y.-H. Choi, B. Nasseri, and R. Hetzer A heart full of stem cells: the spectrum of myocardial progenitor cells in the postnatal heart Therapeutic Advances in Cardiovascular Disease, June 1, 2009; 3(3): 215 - 229. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Miyamoto, M. Rubio, and M. A. Sussman Nuclear and mitochondrial signalling Akts in cardiomyocytes Cardiovasc Res, May 1, 2009; 82(2): 272 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Muraski, K. M. Fischer, W. Wu, C. T. Cottage, P. Quijada, M. Mason, S. Din, N. Gude, R. Alvarez Jr, M. Rota, et al. Pim-1 kinase antagonizes aspects of myocardial hypertrophy and compensation to pathological pressure overload PNAS, September 16, 2008; 105(37): 13889 - 13894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Siddiqi, N. Gude, T. Hosoda, J. Muraski, M. Rubio, G. Emmanuel, J. Fransioli, S. Vitale, C. Parolin, D. D'Amario, et al. Myocardial Induction of Nucleostemin in Response to Postnatal Growth and Pathological Challenge Circ. Res., July 3, 2008; 103(1): 89 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Evans-Anderson, C. M. Alfieri, and K. E. Yutzey Regulation of Cardiomyocyte Proliferation and Myocardial Growth During Development by FOXO Transcription Factors Circ. Res., March 28, 2008; 102(6): 686 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tateishi, E. Ashihara, N. Takehara, T. Nomura, S. Honsho, T. Nakagami, S. Morikawa, T. Takahashi, T. Ueyama, H. Matsubara, et al. Clonally amplified cardiac stem cells are regulated by Sca-1 signaling for efficient cardiovascular regeneration J. Cell Sci., May 15, 2007; 120(10): 1791 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Rosenberg and J. J. Oppenheim Interview with Dr. Maurizio C. Capogrossi regarding Pivotal Advance: High-mobility group box 1 protein--a cytokine with a role in cardiac repair J. Leukoc. Biol., January 1, 2007; 81(1): 38 - 40. [Full Text] [PDF]
|
|
|
|
|
|
D. Catalucci and G. Condorelli Effects of Akt on Cardiac Myocytes: Location Counts Circ. Res., August 18, 2006; 99(4): 339 - 341. [Full Text] [PDF]
|
|