Circulation Research. 2003
Published online before print May 1, 2003, doi: 10.1161/01.RES.0000075089.39335.8C
Published online before print May 1, 2003, doi: 10.1161/01.RES.0000075089.39335.8C
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Submitted on December 27, 2002
Revised on April 23, 2003
Accepted on April 24, 2003
Physiological Coupling of Donor and Host Cardiomyocytes After Cellular Transplantation
Michael Rubart ;
From the Wells Center for Pediatric Research and Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Ind.
* To whom correspondence should be addressed. E-mail: ljfield{at}iupui.edu.
Cellular transplantation has emerged as a potential approach to treat diseased hearts. Although cell transplantation can affect global heart function, it is not known if this results directly via functional integration of donor myocytes or indirectly via enhanced revascularization and/or altered postinjury remodeling. To determine the degree to which donor cardiomyocytes are able to functionally integrate with the host myocardium, fetal transgenic cardiomyocytes expressing enhanced green fluorescent protein were transplanted into the hearts of nontransgenic adult mice. Two-photon molecular excitation laser scanning microscopy was then used to simultaneously image cellular calcium transients in donor and host cells within the intact recipient hearts. Calcium transients in the donor cardiomyocytes were synchronous with and had kinetics indistinguishable from those of neighboring host cardiomyocytes. These results strongly suggest that donor cardiomyocytes functionally couple with host cardiomyocytes and support the notion that transplanted cardiomyocytes can form a functional syncytium with the host myocardium.
Key words: cellular transplantation • heart regeneration
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