Circulation Research. 2002
Published online before print December 26, 2002, doi: 10.1161/01.RES.0000054200.44505.AB
Published online before print December 26, 2002, doi: 10.1161/01.RES.0000054200.44505.AB
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Submitted on November 6, 2002
Revised on December 9, 2002
Accepted on December 13, 2002
Role for Hydrogen Peroxide in Flow-Induced Dilation of Human Coronary Arterioles
Hiroto Miura *;
From the Department of Veterans Affairs Medical Center, and Department of Medicine and Cardiovascular Research Center (H.M., J.J.B., D.D.G.), Milwaukee, Wis; Department of Microbiology and Molecular Genetics (G.N.), Medical College of Wisconsin, Milwaukee, Wis; and the 2nd Department of Internal Medicine (T.S., M.M.), Akita University, Akita City, Japan.
* To whom correspondence should be addressed. E-mail: hmiura{at}mcw.edu.
Flow-induced dilation (FID) is dependent largely on hyperpolarization of vascular smooth muscle cells (VSMCs) in human coronary arterioles (HCA) from patients with coronary disease. Animal studies show that shear stress induces endothelial generation of hydrogen peroxide (H2O2), which is proposed as an endothelium-derived hyperpolarizing factor (EDHF). We tested the hypothesis that H2O2 contributes to FID in HCA. Arterioles (135±7 µm, n=71) were dissected from human right atrial appendages at the time of cardiac surgery and cannulated with glass micropipettes. Changes in internal diameter and membrane potential of VSMCs to shear stress, H2O2, or to papaverine were recorded with videomicroscopy. In some vessels, endothelial H2O2 generation to shear stress was monitored directly using confocal microscopy with 2',7'-dichlorofluorescin diacetate (DCFH) or using electron microscopy with cerium chloride. Catalase inhibited FID (%max dilation; 66±8 versus 25±7%; P<0.05, n=6), whereas dilation to papaverine was unchanged. Shear stress immediately increased DCFH fluorescence in the endothelial cell layer, whereas treatment with catalase abolished the increase in fluorescence. Electron microscopy with cerium chloride revealed shear stress-induced increase in cerium deposition in intimal area surrounding endothelial cells. Exogenous H2O2 dilated (%max dilation; 97±1%, ED50; 3.0±0.7x10-5 mol/L) and hyperpolarized HCA. Dilation to H2O2 was reduced by catalase, 40 mmol/L KCl, or charybdotoxin plus apamin, whereas endothelial denudation, deferoxamine, 1H-1,2,4-oxadiazole-[4,3-a]quinoxalin-1-one, or glibenclamide had no effect. These data provide an evidence that shear stress induces endothelial release of H2O2 and are consistent with the idea that H2O2 is an EDHF that contributes to FID in HCA from patients with heart disease. The full text of this article is available at http://www.circresaha.org.
Key words: human • coronary microcirculation • flow-induced dilation • hydrogen peroxides
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