Published online before print October 3, 2002, doi: 10.1161/01.RES.0000039534.18114.D9
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on May 31, 2002
Revised on September 4, 2002
Accepted on September 19, 2002
Comparison of Ion-Channel Subunit Expression in Canine Cardiac Purkinje Fibers and Ventricular Muscle
Wei Han ;
From the Department of Medicine and Research Center, Montreal Heart Institute and University of Montreal (W.H., W.B., Z.W., S.N.), and the Department of Pharmacology, McGill University (W.H., S.N.), Montreal, Québec, Canada.
* To whom correspondence should be addressed. E-mail: nattel{at}icm.umontreal.ca.
Although Purkinje fibers (PFs) play an important role in cardiac electrophysiology, almost nothing is known about the expression of ion-channel subunits in PFs. We applied competitive reverse transcription-polymerase chain reaction, Western blotting, and immunocytochemistry to compare the expression of ion-channel subunit mRNA and protein in canine PFs versus ventricular muscle (VM). For transient outward current-related subunits, Kv4.2 was not detected, and Kv1.4 expression was extremely low. Kv4.3 expression was of the same order for VM and PFs. The tetraethylammonium chloride-sensitive subunit Kv3.4 was expressed much more strongly in PFs than in VM, and Kv channel-interacting protein transcript expression was 25-fold stronger in VM than in PFs. For delayed rectifiers, ERG and KvLQT1 expression was lower in PFs at both mRNA and protein levels. Although minK transcripts were more numerous in PFs, minK protein was significantly more strongly expressed in VM. L-type Ca2+ current 
-subunit (CaV1.2) and Na+-Ca2+ exchanger proteins were more strongly expressed in VM than in PFs. For T-type Ca2+ current, CaV3.1, CaV3.2, and CaV3.3 transcripts were all more strongly expressed in PFs. For the nonselective cation current, hyperpolarization-activated cation channel 1 (HCN1) expression was subquantifiable, HCN2 transcript expression was comparable in PFs and VM, and HCN4 mRNA expression was strong in PFs but below the detection threshold in VM. HCN2 and HCN4 protein expression was much stronger in PFs than in VM. We conclude that ion-channel subunit expression in PFs differs from that in VM in ways that are consistent with, and shed light on the molecular basis of, well-recognized fundamental PF ionic properties.
Key words: molecular biology • cardiac arrhythmias • ion currents
This article has been cited by other articles:
 |
|
 |
|
  X. Luo, H. Lin, Z. Pan, J. Xiao, Y. Zhang, Y. Lu, B. Yang, and Z. Wang Down-regulation of miR-1/miR-133 Contributes to Re-expression of Pacemaker Channel Genes HCN2 and HCN4 in Hypertrophic Heart J. Biol. Chem., July 18, 2008; 283(29): 20045 - 20052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Mangoni and J. Nargeot Genesis and Regulation of the Heart Automaticity Physiol Rev, July 1, 2008; 88(3): 919 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sun, D. Varela, D. Chartier, P. C. Ruben, S. Nattel, G. W. Zamponi, and N. Leblanc Differential Interactions of Na+ Channel Toxins with T-type Ca2+ Channels J. Gen. Physiol., July 1, 2008; 132(1): 101 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Holycross, M. Kukielka, Y. Nishijima, R. A. Altschuld, C. A. Carnes, and G. E. Billman Exercise training normalizes beta-adrenoceptor expression in dogs susceptible to ventricular fibrillation Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2702 - H2709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Gaborit, S. Le Bouter, V. Szuts, A. Varro, D. Escande, S. Nattel, and S. Demolombe Regional and tissue specific transcript signatures of ion channel genes in the non-diseased human heart J. Physiol., July 15, 2007; 582(2): 675 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nikitina, Z.-D. Zhang, A. Kawashima, B. S. Jahromi, V. A. Bouryi, M. Takahashi, A. Xie, and R. L. Macdonald Voltage-dependent calcium channels of dog basilar artery J. Physiol., April 15, 2007; 580(2): 523 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nattel, A. Maguy, S. Le Bouter, and Y.-H. Yeh Arrhythmogenic Ion-Channel Remodeling in the Heart: Heart Failure, Myocardial Infarction, and Atrial Fibrillation Physiol Rev, April 1, 2007; 87(2): 425 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Rosati, W. Dun, M. Hirose, P. A. Boyden, and D. McKinnon Molecular basis of the T- and L-type Ca2+ currents in canine Purkinje fibres J. Physiol., March 1, 2007; 579(2): 465 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu, H. Dobrzynski, J. Yanni, M. R. Boyett, and M. Lei Organisation of the mouse sinoatrial node: structure and expression of HCN channels Cardiovasc Res, March 1, 2007; 73(4): 729 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Harrell, S. Harbi, J. F. Hoffman, J. Zavadil, and W. A. Coetzee Large-scale analysis of ion channel gene expression in the mouse heart during perinatal development Physiol Genomics, February 12, 2007; 28(3): 273 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lengyel, L. Virag, T. Biro, N. Jost, J. Magyar, P. Biliczki, E. Kocsis, R. Skoumal, P. P. Nanasi, M. Toth, et al. Diabetes mellitus attenuates the repolarization reserve in mammalian heart Cardiovasc Res, February 1, 2007; 73(3): 512 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamamoto, H. Dobrzynski, J. Tellez, R. Niwa, R. Billeter, H. Honjo, I. Kodama, and M. R. Boyett Extended atrial conduction system characterised by the expression of the HCN4 channel and connexin45 Cardiovasc Res, November 1, 2006; 72(2): 271 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Han, S. Nattel, T. Noguchi, and A. Shrier C-terminal Domain of Kv4.2 and Associated KChIP2 Interactions Regulate Functional Expression and Gating of Kv4.2 J. Biol. Chem., September 15, 2006; 281(37): 27134 - 27144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Obreztchikova, K. W. Patberg, A. N. Plotnikov, N. Ozgen, I. N. Shlapakova, A. V. Rybin, E. A. Sosunov, P. Danilo Jr., E. P. Anyukhovsky, R. B. Robinson, et al. IKr contributes to the altered ventricular repolarization that determines long-term cardiac memory Cardiovasc Res, July 1, 2006; 71(1): 88 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Patel and D. L. Campbell Transient outward potassium current, 'Ito', phenotypes in the mammalian left ventricle: underlying molecular, cellular and biophysical mechanisms J. Physiol., November 15, 2005; 569(1): 7 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Szentadrassy, T. Banyasz, T. Biro, G. Szabo, B. I. Toth, J. Magyar, J. Lazar, A. Varro, L. Kovacs, and P. P. Nanasi Apico-basal inhomogeneity in distribution of ion channels in canine and human ventricular myocardium Cardiovasc Res, March 1, 2005; 65(4): 851 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. White and W. C. Claycomb Embryonic stem cells form an organized, functional cardiac conduction system in vitro Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H670 - H679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Doronin, I. A. Potapova, Z. Lu, and I. S. Cohen Angiotensin Receptor Type 1 Forms a Complex with the Transient Outward Potassium Channel Kv4.3 and Regulates Its Gating Properties and Intracellular Localization J. Biol. Chem., November 12, 2004; 279(46): 48231 - 48237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Dun, S. Baba, T. Yagi, and P. A. Boyden Dynamic remodeling of K+ and Ca2+ currents in cells that survived in the epicardial border zone of canine healed infarcted heart Am J Physiol Heart Circ Physiol, September 1, 2004; 287(3): H1046 - H1054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, R. E. W. Fyffe, and L. Lu Identification of a Kv3.4 Channel in Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1796 - 1803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jiang, M. Zhang, D. G. Tang, H. F. Clemo, J. Liu, D. Holwitt, V. Kasirajan, A. L. Pond, E. Wettwer, and G.-N. Tseng KCNE2 Protein Is Expressed in Ventricles of Different Species, and Changes in Its Expression Contribute to Electrical Remodeling in Diseased Hearts Circulation, April 13, 2004; 109(14): 1783 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pourrier, S. Zicha, J. Ehrlich, W. Han, and S. Nattel Canine Ventricular KCNE2 Expression Resides Predominantly in Purkinje Fibers Circ. Res., August 8, 2003; 93(3): 189 - 191. [Abstract] [Full Text] [PDF]
|
|