Published online before print May 9, 2002, doi: 10.1161/01.RES.0000020861.20064.7E
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Submitted on March 6, 2002
Revised on April 23, 2002
Accepted on April 24, 2002
Young Adult Bone Marrow--Derived Endothelial Precursor Cells Restore Aging-Impaired Cardiac Angiogenic Function
Jay M. Edelberg *;
From the Departments of Medicine (J.M.F., L.T., K.H., S.R.) and Cell and Developmental Biology (J.M.E., D.L.), Weill Medical College of Cornell University, New York, NY; and the Department of Pediatrics (D.L.), Memorial Sloan-Kettering Cancer Center, New York, NY.
* To whom correspondence should be addressed. E-mail: jme2002{at}med.cornell.edu.
Delivery of young bone marrow--derived stem cells offers a novel approach for restoring the impaired senescent cardiac angiogenic function that may underlie the increased morbidity and mortality associated with ischemic heart disease in older individuals. Recently, we reported that alterations in endothelial cells of the aging heart lead to a dysregulation in the cardiac myocyte platelet-derived growth factor (PDGF)-B--induced paracrine pathway, which contributes to impaired cardiac angiogenic function. Based on these results, we hypothesized that cellular restoration of the PDGF pathway by bone marrow--derived endothelial precursor cells (EPCs) could reverse the aging-associated decline in angiogenic activity. In vitro studies revealed that young murine (3-month-old) bone marrow--derived EPCs recapitulated the cardiac myocyte--induced expression of PDGF-B, whereas EPCs from the bone marrow of aging mice (18-month-old) did not express PDGF-B when cultured in the presence of cardiac myocytes. Transplantation of young, but not old, genetically marked syngeneic bone marrow cells into intact, unirradiated aging mice that populated the endogenous senescent murine bone marrow incorporated into the neovasculature of subsequently transplanted syngeneic neonatal myocardium. Moreover, the young bone marrow--derived EPCs restored the senescent host angiogenic PDGF-B induction pathway and cardiac angiogenesis, with graft survival and myocardial activity in the aging murine host (cardiac allograft viability: 3-month-old controls, 8/8; 18-month-old controls, 1/8; 18-month-old donors receiving bone marrow from 3-month-old mice, 15/16; or 18-month-old mice, 0/6; P<0.05). These results may offer a foundation for the development of novel therapies for the prevention and treatment of cardiovascular disease associated with aging. The full text of this article is available at http://www.circresaha.org.
Key words: endothelium • heart • angiogenesis • aging • bone marrow transplantation
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