Published online before print February 28, 2002, doi: 10.1161/01.RES.0000013796.73742.C8
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Submitted on October 9, 2001
Revised on February 13, 2002
Accepted on February 13, 2002
CADASIL Notch3 Mutant Proteins Localize to the Cell Surface and Bind Ligand
Talin Haritunians ;
From the Departments of Human Genetics (T.H., C.S.), Psychiatry and Biobehavioral Sciences (J.B.), and Biological Chemistry (C.H., G.D., C.S., G.W.), University of California, Los Angeles, and Natural Science Division (J.B., D.N.), Pepperdine University, Malibu, Calif.
* To whom correspondence should be addressed. E-mail: schanen{at}ucla.edu.
Cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a vascular dementia arising from abnormal arteriolar vascular smooth muscle cells. CADASIL results from mutations in Notch3 that alter the number of cysteine residues in the extracellular epidermal growth factor--like repeats, important for ligand binding. It is not known whether CADASIL mutations lead to loss or gain of Notch3 receptor function. To examine the functional consequences of CADASIL mutations, we engineered 4 CADASIL-like mutations into rat Notch3 and have shown that the presence of an unpaired cysteine does not impair cell-surface expression or ligand binding.
Key words: CADASIL • Notch3 • vascular dementia • epidermal growth factor--like repeat • transmembrane receptor
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