Published online before print February 7, 2002, doi: 10.1161/01.RES.0000012666.42782.30
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on October 23, 2001
Revised on January 29, 2002
Accepted on January 30, 2002
Intramolecular Interaction of SUR2 Subtypes for Intracellular ADP-Induced Differential Control of KATP Channels
Kenji Matsushita ;
From the Departments of Pharmacology II (K.M., T.M., A.F., Y.K.) and Ophthalmology (K.M., T.F., Y.T.), Graduate School of Medicine, and the Research Center For Structural Biology (H.N.), Institute for Protein Research, Osaka University, Suita, Osaka, Japan; and the Division of Science of Biological Supramolecular Systems (K.K.), Graduate School of Integrated Science, Yokohama University, Yokohama, Kanagawa, Japan.
* To whom correspondence should be addressed. E-mail: ykurachi{at}pharma2.med.osaka-u.ac.jp.
ATP-sensitive K+ (KATP) channels are composed of sulfonylurea receptors (SURs) and inwardly rectifying Kir6.2-channels. The C-terminal 42 amino acid residues (C42) of SURs are responsible for ADP-induced differential activation of KATP channels in SUR-subtypes. By examining ADP-effect on KATP channels containing various chimeras of SUR2A and SUR2B, we identified a segment of 7 residues at central portion of C42 critical for this phenomenon. A 3-D structure model of the region containing the second nucleotide-binding domain (NBD2) of SUR and C42 was developed based on the structure of HisP, a nucleotide-binding protein forming the bacterial Histidine transporter complex. In the model, the polar and charged residues in the critical segment located within a distance that allows their electrostatic interaction with Arg1344 at the Walker-A loop of NBD2. Therefore, the interaction might be involved in the control of ADP-induced differential activation of SUR2-subtype KATP channels.
Key words: homology modeling • KATP channel • nucleotide binding domain • sulfonylurea receptor • Walker motif
This article has been cited by other articles:
 |
|
 |
|
  M. A. Burke, R. K. Mutharasan, and H. Ardehali The Sulfonylurea Receptor, an Atypical ATP-Binding Cassette Protein, and Its Regulation of the KATP Channel Circ. Res., February 1, 2008; 102(2): 164 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. de Wet, M. G. Rees, K. Shimomura, J. Aittoniemi, A.-M. Patch, S. E. Flanagan, S. Ellard, A. T. Hattersley, M. S. P. Sansom, and F. M. Ashcroft Increased ATPase activity produced by mutations at arginine-1380 in nucleotide-binding domain 2 of ABCC8 causes neonatal diabetes PNAS, November 27, 2007; 104(48): 18988 - 18992. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. V. Zingman, A. E. Alekseev, D. M. Hodgson-Zingman, and A. Terzic ATP-sensitive potassium channels: metabolic sensing and cardioprotection J Appl Physiol, November 1, 2007; 103(5): 1888 - 1893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ehses, R. M. Leonhardt, G. Hansen, and M. R. Knittler Functional Role of C-Terminal Sequence Elements in the Transporter Associated with Antigen Processing J. Immunol., January 1, 2005; 174(1): 328 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Westlake, L. Payen, M. Gao, S. P. C. Cole, and R. G. Deeley Identification and Characterization of Functionally Important Elements in the Multidrug Resistance Protein 1 COOH-terminal Region J. Biol. Chem., December 17, 2004; 279(51): 53571 - 53583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Campbell, P. Proks, J. D. Lippiat, M. S.P. Sansom, and F. M. Ashcroft Identification of a Functionally Important Negatively Charged Residue Within the Second Catalytic Site of the SUR1 Nucleotide-Binding Domains Diabetes, December 1, 2004; 53(suppl_3): S123 - S127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yamada, M. Ishii, H. Hibino, and Y. Kurachi Mutation in Nucleotide-Binding Domains of Sulfonylurea Receptor 2 Evokes Na-ATP-Dependent Activation of ATP-Sensitive K+ Channels: Implication for Dimerization of Nucleotide-Binding Domains to Induce Channel Opening Mol. Pharmacol., October 1, 2004; 66(4): 807 - 816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamada and Y. Kurachi The Nucleotide-Binding Domains of Sulfonylurea Receptor 2A and 2B Play Different Functional Roles in Nicorandil-Induced Activation of ATP-Sensitive K+ Channels Mol. Pharmacol., May 1, 2004; 65(5): 1198 - 1207. [Abstract] [Full Text]
|
|
|
|
|
|
L. V. Zingman, D. M. Hodgson, P. H. Bast, G. C. Kane, C. Perez-Terzic, R. J. Gumina, D. Pucar, M. Bienengraeber, P. P. Dzeja, T. Miki, et al. Kir6.2 is required for adaptation to stress PNAS, October 1, 2002; 99(20): 13278 - 13283. [Abstract] [Full Text] [PDF]
|
|