Reviews |
From Molecular Cardiology, Department of Internal Medicine IV, University of Frankfurt, Frankfurt, Germany.
Correspondence to Stefanie Dimmeler, PhD, Molecular Cardiology, Dept of Internal Medicine IV, University of Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany. E-mail Dimmeler{at}em.uni-frankfurt.de
This Review is part of a thematic series on Angiogenesis, which includes the following articles:
Endothelial Progenitor Cells: Characterization and Role in Vascular Biology
Bone MarrowDerived Cells for Enhancing Collateral Development: Mechanisms, Animal Data, and Initial Clinical Experiences
Arteriogenesis
Innate Immunity and Angiogenesis
Syndecans
Growth Factors and Blood Vessels: Differentiation and Maturation
Ralph Kelly Guest Editor
| Abstract |
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Key Words: progenitor cells neovascularization vasculogenesis angiogenesis endothelial cells
| Introduction |
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Overall, controversy exists with respect to the identification and the origin of endothelial progenitor cells, which are isolated from peripheral blood mononuclear cells by cultivation in medium favoring endothelial differentiation. In peripheral blood mononuclear cells, several possible sources for endothelial cells may exist: (1) the rare number of hematopoietic stem cells, (2) myeloid cells, which may differentiate to endothelial cells under the cultivation selection pressure, (3) other circulating progenitor cells (eg, "side population" cells), and (4) circulating mature endothelial cells, which are shed off the vessel wall6 and adhere to the culture dishes. First evidence that there is more than one endothelial progeny within the circulating blood was provided by Hebbel and colleagues, who showed that morphological and functional distinct endothelial cell populations can be grown out of peripheral blood mononuclear cells.7 They stratified the different circulating endothelial cells according to their growth characteristics and morphological appearance as "spindle-like cells," which have a low proliferative capacity, and outgrowing cells. Because the outgrowing cells showed a high proliferative potential and originated predominantly from the bone marrow donors, they were considered as circulating angioblasts.7 The authors speculated that the spindle-like cells may likely represent mature endothelial cells, which are shed off the vessel wall. However, this hypothesis is difficult to test and has not yet been proven thus far.
Experimentally, preplating may be a way to reduce the heterogeneity of the cultivated EPCs, because this excludes rapidly adhering cells such as differentiated monocytic or possible mature endothelial cells.2 However, these protocols do not eliminate myeloid and nonhematopoietic progenitor cells, which may contribute to the ex vivo cultivated cells. There is increasing evidence that myeloid cells can give rise to endothelial cells as well. Specifically, CD14+/CD34 myeloid cells can coexpress endothelial markers and form tube-like structures ex vivo.8 Additionally, ex vivo expansion of purified CD14+ mononuclear cells yielded cells with an endothelial characteristic, which incorporated in newly formed blood vessels in vivo.9 These data would suggest that myeloid cells can differentiate (or transdifferentiate) to the endothelial lineage. Interestingly, lineage tracking showed that myeloid cells are the hematopoietic stem cell-derived intermediates, which contribute to muscle regeneration,10 suggesting that myeloid intermediates may be part of the repair capacity after injury. Moreover, a subset of human peripheral blood monocytes acts as pluripotent stem cells.11
Of note, a specific problem arises when cells are ex vivo expanded and cultured, because the culture conditions (culture supplements such as FCS and cytokines, plastic) rapidly changes the phenotype of the cells. For example, supplementation of the medium with statins increased the number of endothelial cell colonies isolated from mononuclear cells.12 Moreover, continuous cultivation was shown to increase endothelial marker protein expression.13 This may explain why different groups may obtain cells with different surface factor profile and functional activity although similar protocols were used for cultivation.9,1416 Moreover, the interaction of cells within a heterogeneous mixture of cells such as the mononuclear cells from the blood may impact the yield and the functional activity of the cultivated cells.17
Generally, several studies suggested that other cell populations beside hematopoietic stem cells also can give rise to endothelial cells (Figure 1). Thus, non-bone marrow-derived cells have been shown to replace the endothelial cells in grafts.18 In addition, adult bone marrow-derived stem/progenitor cells such as the side population cells and multipotent adult progenitor cells, which are distinct from hematopoietic stem cells, have also been shown to differentiate to the endothelial lineage.19,20 Recently, tissue-resident stem cells have been isolated from the heart, which are capable to differentiate to the endothelial lineage.21 These data support the notion that it will be difficult to define the "true" endothelial progenitor cells. Overall, the field is reminiscent to immunology, where T-cells initially were defined as one cell population. However, the functional characterization (eg, cytokine release and response to stimuli) helped to identify novel T-cell subpopulations with distinct functions and capacities. Hopefully, better profiling of distinct cell populations and fate mapping studies will help to identify markers, which distinguish the circulating endothelial precursor within the blood and bone marrow/non-bone marrow-derived endothelial cells.
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| Role of EPCs in Neovascularization |
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Besides models of peripheral ischemia (hind limb ischemia), the angiogenic potential of EPCs was also investigated in animal models of tumor angiogenesis. Thereby, the inhibition of VEGF-responsive bone marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth.28 The use of various different models, cell numbers, and species limits the comparability of the efficiency of distinct cell populations. However, the overall functional improvement appear similar, when isolated human CD34+, CD133+, EPC, MAPC, or murine Sca-1+ cells were used.4,9,15,20,23,25,2932 Likewise, early spindle-like cells and late outgrowing EPCs showed comparable in vivo vasculogenic capacity.33 These results suggest that the functional activity of the cells to augment neovascularization is rather independent of the type of (endothelial) progenitor cell used. However, the CD34 fractions of freshly isolated bone marrow- or blood-derived mononuclear cells showed a reduced incorporation and functional activity.24,29 These data indicate that the number of cells capable to augment neovascularization is low in this crude fraction of freshly isolated uncultivated CD34 cells. Remarkably, terminally differentiated mature endothelial cells (HMVECs, GEAECs, and SVECs) did not improve neovascularization15,24,33 suggesting that a not-yet-defined functional characteristic (eg, chemokine or integrin receptors mediating homing) is essential for EPC-mediated augmentation of blood flow after ischemia.
The functional capacity of EPCs to augment blood flow further does not appear to be solely attributable to a monocytic phenotype. Ex vivo cultivated EPCs from CD14+ mononuclear cells or CD14 mononuclear cell starting population improved neovascularization to a similar extent, whereas the same number of freshly isolated mononuclear cells taken from the starting culture did not.9 Interestingly, these experimental data are supported by first clinical trials showing that freshly isolated mononuclear cells are not well suited to improve neovascularization in patients with peripheral vascular diseases.26 However, monocytic cells may play a crucial role in collateral growth (arteriogenesis). Thus, the attraction of monocytic cells by monocyte chemoattractant protein-1 (MCP-1) enhanced arteriogenesis.34 Moreover, depletion of the monocytes reduced PlGF-induced arteriogenesis.35 A therapeutic benefit of monocyte infusion on arteriogenesis was demonstrated under conditions of monocyte deficiency induced by chemical depletion.36 These data suggest that monocytic cells are necessary for arteriogenesis and possibly neovascularization. For therapeutic application, the local enhancement of monocyte recruitment might be better suited than systemic infusion of monocytic cells, which only leads to a relatively minor increase in the number of circulating monocytes.
Mechanisms by Which EPC Improve Neovascularization
Although the role of EPCs in neovascularization has been convincingly shown by several groups, the question remains: how do EPCs improve neovascularization?
Bone marrow transplantation of genetically modified cells (rosa-26, GFP, lacZ) was used to assess the incorporation of bone marrow-derived EPC into tissues. The basal incorporation rate of progenitor cells without tissue injury is extremely low.37 In ischemic tissue, the incorporation rate of genetically labeled bone marrow-derived cells, which coexpress endothelial marker proteins, differs from 0% to 90% incorporation.19,28,3741 Likewise, the extent of incorporation of bone marrow-derived cells in cerebral vessels after stroke varies in the literature.4244 Whereas two studies reported positive vessels with an average of 34% endothelial marker expressing bone marrow-derived cells,42,43 other groups could not detect endothelial marker expressing cells.44 High amounts (>50%) were predominantly detected in models of tumor angiogenesis.28,40 Some studies only detected bone marrow-derived cells adjacent to vessels, which do not express endothelial marker proteins.41,45 A reasonable explanation might be that the model of ischemia (eg, intensity of injury or ischemia)46 significantly influences the incorporation rate. A minor ischemia might not as profoundly induce a mobilization of bone marrow-derived endothelial progenitor cells and, thus, may lead to a lower percentage of incorporation of bone marrow-derived progenitor cells. The efficiency of engraftment may additionally differ between distinct progenitor subpopulations (pure hematopoietic stem cells versus complete bone marrow cells). Indeed, therapeutic application of cells by intravenous infusion of ex vivo purified bone marrow mononuclear cells or expanded endothelial progenitor cells led to a higher incorporation rate (
7% to 20% incorporation rate) as compared with the endogenously mobilized bone marrow-engrafted cells (
2%).9,47
However, the number of incorporated cells with an endothelial phenotype into ischemic tissues is generally quite low. How can such a small number of cells increase neovascularization? A possible explanation might be that the efficiency of neovascularization may not solely be attributable to the incorporation of EPCs in newly formed vessels, but may also be influenced by the release of proangiogenic factors in a paracrine manner. Indeed, the deletion of Tie-2-positive bone marrow-derived cells through activation of a suicide gene blocked tumor angiogenesis, although these cells are not integrated into the tumor vessels but are detected adjacent to the vessel.41 Thus, EPCs may act similar to monocytes/macrophages, which can increase arteriogenesis by providing cytokines and growth factors. Indeed, EPCs cultivated from different sources showed a marked expression of growth factors such as VEGF, HGF, and IGF-1 (C.U., unpublished data, 2004). Moreover, adherent monocytic cells, which were cultivated under similar conditions, but do not express endothelial marker proteins, also release VEGF, HGF, and G-CSF.14 The release of growth factors in turn may influence the classical process of angiogenesis, namely the proliferation and migration as well as survival of mature endothelial cells.48 However, EPCs additionally incorporated into the newly formed vessel structures and showed endothelial marker protein expression in vivo. In contrast, infusion of macrophages, which are known to release growth factors,49,50 but were not incorporated into vessel-like structures, induced only a slight increase in neovascularization after ischemia, indicatingbut not provingthat the capacity of EPCs to physically contribute to vessel-like structures may contribute to their potent capacity to improve neovascularization.9 Further studies will have to be designed to elucidate the contribution of physical incorporation, paracrine effects and possible effects on vessel remodeling and facilitating vessel branching to EPC-mediated improvement of neovascularization.
| EPCs and Endothelial Regeneration |
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A rapid regeneration of the endothelial monolayer may prevent restenosis development by endothelial synthesis of antiproliferative mediators such as nitric oxide. Indeed, enhanced incorporation of ß-galactosidase-positive, bone marrow-derived cells was associated with an accelerated reendothelialization and reduction of restenosis.51,52 Similar results were reported by Griese et al, who demonstrated that infused peripheral blood monocyte-derived EPC home to bioprosthetic grafts and to balloon-injured carotid arteries, the latter being associated with a significant reduction in neointima deposition.54 Likewise, infusion of bone marrow-derived CD34/CD14+ mononuclear cells, which are not representing the population of the "classical hemangioblast," contributed to endothelial regeneration.13 The regenerated endothelium was functionally active as shown by the release of NO,13 which is supposed to be one of the major vasculoprotective mechanisms. Consistently, neointima development was significantly reduced after cell infusion.13 Whereas the regeneration of the endothelium by EPCs protects lesion formation, bone marrow-derived stem/progenitor cells may also contribute to plaque angiogenesis, thereby potentially facilitating plaque instability.55 However, in a recent study, no influence of bone marrow cell infusion on plaque composition was detected in nonischemic mice.56 An increase in plaque size was only detected in the presence of ischemia, suggesting that ischemia-induced release of growth factors predominantly accounts for this effect.56
Overall, these studies implicate that regardless of the origin of circulating endothelial progenitor cells, this pool of circulating endothelial cells may exert an important function as an endogenous repair mechanism to maintain the integrity of the endothelial monolayer by replacing denuded parts of the artery (Figure 2). One can speculate that these cells may also regenerate the low grade endothelial damage by ongoing induction of endothelial cell apoptosis induced by risk factors for coronary artery disease (see review).57 The maintenance of the endothelial monolayer may prevent thrombotic complications and atherosclerotic lesion development. Although this concept has not yet been proven, several hints from recently presented data are supportive. Thus, transplantation of ApoE/ mice with wild-type bone marrow reduced atherosclerotic lesion formation.58 Moreover, various risk factors for coronary artery disease, such as diabetes, hypercholesterolemia, hypertension, and smoking, affect the number and functional activity of EPCs in healthy volunteers59 and in patients with coronary artery disease.60 Likewise, diabetic mice and patients were characterized by reduced functional activity of EPCs.6163 In addition, factors that reduce cardiovascular risk such as statins38,51,52,64 or exercise65 elevate EPC levels, which contribute to enhanced endothelial repair. The balance of atheroprotective and proatherosclerotic factors, thus, may influence EPC levels and subsequently reendothelialization capacity.
| Mobilization of EPCs |
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First evidence for potential pharmacological modulation of systemic EPC levels by atheroprotective drugs came from studies using HMG-CoA reductase inhibitors (statins). Statins were shown to increase the number and the functional activity of EPCs in vitro,38,76 in mice,38,76 and in patients with stable coronary artery disease.64 The increase in EPC numbers was associated with increased bone marrow-derived cells after balloon injury and accelerated endothelial regeneration.51,52 Although statins were shown to increase the number of stem cells within the bone marrow, the mechanism for enhancing EPC numbers and function may additionally include an increase in proliferation, mobilization, and prevention of EPC senescence and apoptosis.12,38,76 Interestingly, recent studies additionally demonstrated that estrogen increased the levels of circulating EPCs.77,78 Moreover, exercise augmented EPC levels in mice and in men.65 The molecular signaling pathways have not been identified thus far. However, several studies indicate that the activation of the PI3K/Akt pathway, which has first been shown to be activated in mature endothelial cells by statins,79 may also play an important role in statin-induced increase in EPC levels.12,76 Likewise, VEGF, EPO, estrogen, and exercise are well known to augment the PI3K/Akt-pathway. Thus, these factors may share some common signaling pathways. Given that recent data showed that eNOS is essential for mobilization of bone marrow-derived stem and progenitor cells,47 one may speculate that these stimuli may increase progenitor cell mobilization by PI3K/Akt-dependent activation of the NO-synthase within the bone marrow stromal cells. Indeed, exercise and VEGF-stimulated EPC mobilization was blunted in eNOS/ mice.47,65
| Mechanism of Homing and Differentiation |
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Adhesion and Transendothelial Migration
The initial step of homing of progenitor cells to ischemic tissue involves adhesion of progenitor cells to endothelial cells activated by cytokines and ischemia and the transmigration of the progenitor cells through the endothelial cell monolayer.80 Integrins are known to mediate the adhesion of various cells including hematopoietic stem cells and leukocytes to extracellular matrix proteins and to endothelial cells.8183 Integrins capable of mediating cell-cell interactions are the ß2-integrins and the
4ß1-integrin. ß1-Integrins are expressed by various cell types including endothelial cells and hematopoietic cells, whereas ß2-integrins are found preferentially on hematopoietic cells.84 Because adhesion to endothelial cells and transmigration events are involved in the in vivo homing of stem cells to tissues with active angiogenesis,80 integrins such as the ß2-integrins and the
4ß1-integrin may be involved in the homing of progenitor cells to ischemic tissues. Consistent with the high expression of ß2-integrins on hematopoietic stem/progenitor cells, ß2-integrins mediate adhesion and transmigration of hematopoietic stem/progenitor cells.85,86 ß2-Integrins (CD18/CD11) are expressed on peripheral blood-derived EPCs and are required for EPC-adhesion to endothelial cells and transendothelial migration in vitro (S.D., personal communication, 2004). Moreover, hematopoietic stem cells (Sca-1+/lin) lacking ß2-integrins showed reduced homing and a lower capacity to improve neovascularization after ischemia (S.D., personal communication, 2004). Interestingly, the homing of inflammatory cells during pneumonia or myocardial ischemia in ß2-integrin-deficient mice is mediated by the
4ß1-integrin87,88 suggesting that deficiency of ß2-integrins can in part be compensated by the
4ß1-integrin. Moreover, conditional deletion of the
4-integrin selectively inhibited the homing of hematopoietic stem/progenitor cells to the bone marrow but not to the spleen,89 suggesting that the homing of progenitor cells to different tissues is dependent on distinct adhesion molecules. Furthermore, in vitro studies showed that MCP-1 stimulated adhesion of bone marrow-derived CD34/CD14+ monocytes to the endothelium was blocked by anti-ß1-integrin antibodies.13 Interestingly, in this study, adhesion of CD34/CD14+ monocytes isolated from the peripheral blood to endothelial cells was less affected by MCP-1 and was not blocked by anti-ß1-integrin antibodies.13 Finally, the initial cell arrest of embryonic progenitor cell homing during tumor angiogenesis was suggested to be mediated by E- and P-selectin and P-selectin glycoprotein ligand-1.80 Yet, it is important to underscore that this study was performed with embryonic endothelial progenitor cells. It is conceivable that different cell types may use distinct mechanisms for homing to sites of angiogenesis.
Cell-cell contacts and transmigration events might be less important for the reendothelialization of denuded arteries (in contrast to homing of progenitor cells to ischemic tissues). With respect to endothelial progenitor cells, studies investigated the contribution of integrins to reendothelialization, which is mainly driven by adhesion to extracellular matrix proteins. Adhesion of EPCs to denuded vessels appears to be mediated by vitronectin-receptors (
vß3- and
vß5-integrins). Thus, inhibition of
vß3- and
vß5-integrins with cyclic RGD peptides blocked reendothelialization of denuded arteries in vivo, suggesting that
vß3- and
vß5-integrins are involved in the reendothelialization of injured carotid arteries.51 However, other integrins such as the ß1-integrins may also mediate adhesion of progenitor cells to extracellular matrix proteins during reendothelialization of denuded arteries.13
Chemotaxis, Migration, and Invasion
Given the low numbers of circulating progenitor cells, chemoattraction may be of utmost importance to allow for recruitment of reasonable numbers of progenitor cells to the ischemic or injured tissue. Various studies examined the factors influencing hematopoietic stem cell engraftment to the bone marrow. These factors include chemokines such as SDF-1,90,91 lipid mediators (sphingosine-1-phosphate),92 as well as factors released by heterologous cells.93 The factors attracting circulating EPCs to the ischemic tissue may be similar. Indeed, SDF-1 has been proven to stimulate recruitment of progenitor cells to the ischemic tissue.31 SDF-1 protein levels were increased during the first days after induction of myocardial infarction.94 Moreover, overexpression of SDF-1 augmented stem cell homing and incorporation into ischemic tissues.31,94 Interestingly, hematopoietic stem cells were shown to be exquisitely sensitive to SDF-1 and did not react to G-CSF or other chemokines (eg, IL-8 and RANTES).91 Moreover, VEGF levels are increased during ischemia and capable to act as a chemoattractive factor to EPCs.68,70,71 Interestingly, the migratory capacity of EPCs or bone marrow cells toward VEGF and SDF-1, respectively, determined the functional improvement of patients after stem cell therapy.95 Beside genes, which are directly upregulated by hypoxia, the invasion of immune competent cells to the ischemic tissue may further augment the levels of various chemokines within the ischemic tissue, such as MCP-1 or interleukins, which can attract circulating progenitor cells.13 Whereas several studies shed some light on the mechanisms regulating attraction of EPCs to ischemic tissue, less is known with respect to migration and tissue invasion. One may speculate that proteases such as cathepsins or metalloproteases may mediate the tissue invasion of EPCs.
Differentiation
Finally, maturation of EPCs to a functional endothelial cell may be important for functional integration in vessels. The genetic cascades that regulate differentiation in the adult system are largely unknown; however, several studies determined the differentiation of the common mesodermal precursor, the hemangioblasts, during embryonic development. Clearly, VEGF and its receptors play a crucial role for stimulating endothelial differentiation in the embryonic development.9698 Likewise, VEGF induces differentiation of endothelial cells in ex vivo culture assays using a variety of adult progenitor populations (CD34+,1 CD133+,4 peripheral blood mononuclear cells).15,76 Studies with embryonic stem cells further revealed that the temporal regulation of Homeobox (Hox) genes might play an important role. Thus, the orphan Hox gene termed Hex (also named Prh) is required for differentiation of the hemangioblast into the definitive hematopoietic progenitors and also affected endothelial differentiation.99 Additionally, the serine/threonine kinase Pim-1 was recently discovered as a VEGF-responsive gene, which contributes to endothelial differentiation out of embryonic stem cells.100
| Conclusion |
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| Acknowledgments |
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| Footnotes |
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| References |
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2. Shi Q, Rafii S, Wu MH, Wijelath ES, Yu C, Ishida A, Fujita Y, Kothari S, Mohle R, Sauvage LR, Moore MA, Storb RF, Hammond WP. Evidence for circulating bone marrow-derived endothelial cells. Blood. 1998; 92: 362367.
3. Peichev M, Naiyer AJ, Pereira D, Zhu Z, Lane WJ, Williams M, Oz MC, Hicklin DJ, Witte L, Moore MA, Rafii S. Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood. 2000; 95: 952958.
4. Gehling UM, Ergun S, Schumacher U, Wagener C, Pantel K, Otte M, Schuch G, Schafhausen P, Mende T, Kilic N, Kluge K, Schafer B, Hossfeld DK, Fiedler W. In vitro differentiation of endothelial cells from AC133-positive progenitor cells. Blood. 2000; 95: 31063112.
5. Handgretinger R, Gordon PR, Leimig T, Chen X, Buhring HJ, Niethammer D, Kuci S. Biology and plasticity of CD133+ hematopoietic stem cells. Ann N|Y Acad Sci. 2003; 996: 141151.[Medline] [Order article via Infotrieve]
6. Mutin M, Canavy I, Blann A, Bory M, Sampol J, Dignat-George F. Direct evidence of endothelial injury in acute myocardial infarction and unstable angina by demonstration of circulating endothelial cells. Blood. 1999; 93: 29512958.
7. Lin Y, Weisdorf DJ, Solovey A, Hebbel RP. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest. 2000; 105: 7177.[Medline] [Order article via Infotrieve]
8. Schmeisser A, Garlichs CD, Zhang H, Eskafi S, Graffy C, Ludwig J, Strasser RH, Daniel WG. Monocytes coexpress endothelial and macrophagocytic lineage markers and form cord-like structures in Matrigel under angiogenic conditions. Cardiovasc Res. 2001; 49: 671680.
9. Urbich C, Heeschen C, Aicher A, Dernbach E, Zeiher AM, Dimmeler S. Relevance of monocytic features for neovascularization capacity of circulating endothelial progenitor cells. Circulation. 2003; 108: 25112516.
10. Camargo FD, Green R, Capetenaki Y, Jackson KA, Goodell MA. Single hematopoietic stem cells generate skeletal muscle through myeloid intermediates. Nat Med. 2003; 9: 15201527.[CrossRef][Medline] [Order article via Infotrieve]
11. Zhao Y, Glesne D, Huberman E. A human peripheral blood monocyte-derived subset acts as pluripotent stem cells. Proc Natl Acad Sci U S A. 2003; 100: 24262431.
12. Assmus B, Urbich C, Aicher A, Hofmann WK, Haendeler J, Rossig L, Spyridopoulos I, Zeiher AM, Dimmeler S. HMG-CoA reductase inhibitors reduce senescence and increase proliferation of endothelial progenitor cells via regulation of cell cycle regulatory genes. Circ Res. 2003; 92: 10491055
13. Fujiyama S, Amano K, Uehira K, Yoshida M, Nishiwaki Y, Nozawa Y, Jin D, Takai S, Miyazaki M, Egashira K, Imada T, Iwasaka T, Matsubara H. Bone marrow monocyte lineage cells adhere on injured endothelium in a monocyte chemoattractant protein-1-dependent manner and accelerate reendothelialization as endothelial progenitor cells. Circ Res. 2003; 93: 980989.
14. Rehman J, Li J, Orschell CM, March KL. Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors. Circulation. 2003; 107: 11641169.
15. Kalka C, Masuda H, Takahashi T, Kalka-Moll WM, Silver M, Kearney M, Li T, Isner JM, Asahara T. Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci U S A. 2000; 97: 34223427.
16. Gulati R, Jevremovic D, Peterson TE, Chatterjee S, Shah V, Vile RG, Simari RD. Diverse origin and function of cells with endothelial phenotype obtained from adult human blood. Circ Res. 2003; 93: 10231025.
17. Rookmaaker MB, Vergeer M, van Zonneveld AJ, Rabelink TJ, Verhaar MC. Endothelial progenitor cells: mainly derived from the monocyte/macrophage-containing CD34- mononuclear cell population and only in part from the hematopoietic stem cell-containing CD34+ mononuclear cell population. Circulation. 2003; 108: e150.[CrossRef][Medline] [Order article via Infotrieve]
18. Hillebrands JL, Klatter FA, van Dijk WD, Rozing J. Bone marrow does not contribute substantially to endothelial-cell replacement in transplant arteriosclerosis. Nat Med. 2002; 8: 194195.[CrossRef][Medline] [Order article via Infotrieve]
19. Jackson KA, Majka SM, Wang H, Pocius J, Hartley CJ, Majesky MW, Entman ML, Michael LH, Hirschi KK, Goodell MA. Regeneration of ischemic cardiac muscle and vascular endothelium by adult stem cells. J Clin Invest. 2001; 107: 13951402.[CrossRef][Medline] [Order article via Infotrieve]
20. Reyes M, Dudek A, Jahagirdar B, Koodie L, Marker PH, Verfaillie CM. Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest. 2002; 109: 337346.[CrossRef][Medline] [Order article via Infotrieve]
21. Beltrami AP, Barlucchi L, Torella D, Baker M, Limana F, Chimenti S, Kasahara H, Rota M, Musso E, Urbanek K, Leri A, Kajstura J, Nadal-Ginard B, Anversa P. Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell. 2003; 114: 763776.[CrossRef][Medline] [Order article via Infotrieve]
22. Isner JM, Asahara T. Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization. J Clin Invest. 1999; 103: 12311236.[Medline] [Order article via Infotrieve]
23. Kawamoto A, Gwon HC, Iwaguro H, Yamaguchi JI, Uchida S, Masuda H, Silver M, Ma H, Kearney M, Isner JM, Asahara T. Therapeutic potential of ex vivo expanded endothelial progenitor cells for myocardial ischemia. Circulation. 2001; 103: 634637.
24. Kocher AA, Schuster MD, Szabolcs MJ, Takuma S, Burkhoff D, Wang J, Homma S, Edwards NM, Itescu S. Neovascularization of ischemic myocardium by human bone-marrow-derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function. Nat Med. 2001; 7: 430436.[CrossRef][Medline] [Order article via Infotrieve]
25. Murohara T, Ikeda H, Duan J, Shintani S, Sasaki K, Eguchi H, Onitsuka I, Matsui K, Imaizumi T. Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization. J Clin Invest. 2000; 105: 15271536.[Medline] [Order article via Infotrieve]
26. Tateishi-Yuyama E, Matsubara H, Murohara T, Ikeda U, Shintani S, Masaki H, Amano K, Kishimoto Y, Yoshimoto K, Akashi H, Shimada K, Iwasaka T, Imaizumi T. Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial. Lancet. 2002; 360: 427435.[CrossRef][Medline] [Order article via Infotrieve]
27. Assmus B, Schachinger V, Teupe C, Britten M, Lehmann R, Dobert N, Grunwald F, Aicher A, Urbich C, Martin H, Hoelzer D, Dimmeler S, Zeiher AM. Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI). Circulation. 2002; 106: 30093017.
28. Lyden D, Hattori K, Dias S, Costa C, Blaikie P, Butros L, Chadburn A, Heissig B, Marks W, Witte L, Wu Y, Hicklin D, Zhu Z, Hackett NR, Crystal RG, Moore MA, Hajjar KA, Manova K, Benezra R, Rafii S. Impaired recruitment of bone-marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth. Nat Med. 2001; 7: 11941201.[CrossRef][Medline] [Order article via Infotrieve]
29. Schatteman GC, Hanlon HD, Jiao C, Dodds SG, Christy BA. Blood-derived angioblasts accelerate blood-flow restoration in diabetic mice. J Clin Invest. 2000; 106: 571578.[Medline] [Order article via Infotrieve]
30. Takahashi T, Kalka C, Masuda H, Chen D, Silver M, Kearney M, Magner M, Isner JM, Asahara T. Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization. Nat Med. 1999; 5: 434438.[CrossRef][Medline] [Order article via Infotrieve]
31. Yamaguchi J, Kusano KF, Masuo O, Kawamoto A, Silver M, Murasawa S, Bosch-Marce M, Masuda H, Losordo DW, Isner JM, Asahara T. Stromal cell-derived factor-1 effects on ex vivo expanded endothelial progenitor cell recruitment for ischemic neovascularization. Circulation. 2003; 107: 13221328.
32. Grant MB, May WS, Caballero S, Brown GA, Guthrie SM, Mames RN, Byrne BJ, Vaught T, Spoerri PE, Peck AB, Scott EW. Adult hematopoietic stem cells provide functional hemangioblast activity during retinal neovascularization. Nat Med. 2002; 8: 607612.[CrossRef][Medline] [Order article via Infotrieve]
33. Hur J, Yoon CH, Kim HS, Choi JH, Kang HJ, Hwang KK, Oh BH, Lee MM, Park YB. Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol. 2004; 24: 288293.
34. van Royen N, Hoefer I, Buschmann I, Kostin S, Voskuil M, Bode C, Schaper W, Piek JJ. Effects of local MCP-1 protein therapy on the development of the collateral circulation and atherosclerosis in Watanabe hyperlipidemic rabbits. Cardiovasc Res. 2003; 57: 178185.
35. Pipp F, Heil M, Issbrucker K, Ziegelhoeffer T, Martin S, van den Heuvel J, Weich H, Fernandez B, Golomb G, Carmeliet P, Schaper W, Clauss M. VEGFR-1-selective VEGF homologue PlGF is arteriogenic: evidence for a monocyte-mediated mechanism. Circ Res. 2003; 92: 378385.
36. Heil M, Ziegelhoeffer T, Pipp F, Kostin S, Martin S, Clauss M, Schaper W. Blood monocyte concentration is critical for enhancement of collateral artery growth. Am J Physiol Heart Circ Physiol. 2002; 283: H2411H2419.
37. Crosby JR, Kaminski WE, Schatteman G, Martin PJ, Raines EW, Seifert RA, Bowen-Pope DF. Endothelial cells of hematopoietic origin make a significant contribution to adult blood vessel formation. Circ Res. 2000; 87: 728730.
38. Llevadot J, Murasawa S, Kureishi Y, Uchida S, Masuda H, Kawamoto A, Walsh K, Isner JM, Asahara T. HMG-CoA reductase inhibitor mobilizes bone marrowderived endothelial progenitor cells. J Clin Invest. 2001; 108: 399405.[CrossRef][Medline] [Order article via Infotrieve]
39. Murayama T, Tepper OM, Silver M, Ma H, Losordo DW, Isner JM, Asahara T, Kalka C. Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo. Exp Hematol. 2002; 30: 967972.[CrossRef][Medline] [Order article via Infotrieve]
40. Garcia-Barros M, Paris F, Cordon-Cardo C, Lyden D, Rafii S, Haimovitz-Friedman A, Fuks Z, Kolesnick R. Tumor response to radiotherapy regulated by endothelial cell apoptosis. Science. 2003; 300: 11551159.
41. De Palma M, Venneri MA, Roca C, Naldini L. Targeting exogenous genes to tumor angiogenesis by transplantation of genetically modified hematopoietic stem cells. Nat Med. 2003; 9: 789795.[CrossRef][Medline] [Order article via Infotrieve]
42. Zhang ZG, Zhang L, Jiang Q, Chopp M. Bone marrow-derived endothelial progenitor cells participate in cerebral neovascularization after focal cerebral ischemia in the adult mouse. Circ Res. 2002; 90: 284288.
43. Hess DC, Hill WD, Martin-Studdard A, Carroll J, Brailer J, Carothers J. Bone marrow as a source of endothelial cells and NeuN-expressing cells After stroke. Stroke. 2002; 33: 13621368.
44. Machein MR, Renninger S, de Lima-Hahn E, Plate KH. Minor contribution of bone marrow-derived endothelial progenitors to the vascularization of murine gliomas. Brain Pathol. 2003; 13: 582597.[Medline] [Order article via Infotrieve]
45. Ziegelhoeffer T, Fernandez B, Kostin S, Heil M, Voswinckel R, Helisch A, Schaper W. Bone marrow-derived cells do not incorporate into the adult growing vasculature. Circ Res. 2004; 94: 230238.
46. Gill M, Dias S, Hattori K, Rivera ML, Hicklin D, Witte L, Girardi L, Yurt R, Himel H, Rafii S. Vascular trauma induces rapid but transient mobilization of VEGFR2(+)AC133(+) endothelial precursor cells. Circ Res. 2001; 88: 167174.
47. Aicher A, Heeschen C, Mildner-Rihm C, Urbich C, Ihling C, Technau-Ihling K, Zeiher AM, Dimmeler S. Essential role of endothelial nitric oxide synthase for mobilization of stem and progenitor cells. Nat Med. 2003; 9: 13701376.[CrossRef][Medline] [Order article via Infotrieve]
48. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med. 1995; 1: 2731.[CrossRef][Medline] [Order article via Infotrieve]
49. Polverini PJ, Cotran PS, Gimbrone MA Jr, Unanue ER. Activated macrophages induce vascular proliferation. Nature. 1977; 269: 804806.[CrossRef][Medline] [Order article via Infotrieve]
50. Berse B, Brown LF, Van de Water L, Dvorak HF, Senger DR. Vascular permeability factor (vascular endothelial growth factor) gene is expressed differentially in normal tissues, macrophages, and tumors. Mol Biol Cell. 1992; 3: 211220.[Abstract]
51. Walter DH, Rittig K, Bahlmann FH, Kirchmair R, Silver M, Murayama T, Nishimura H, Losordo DW, Asahara T, Isner JM. Statin therapy accelerates reendothelialization: a novel effect involving mobilization and incorporation of bone marrow-derived endothelial progenitor cells. Circulation. 2002; 105: 30173024.
52. Werner N, Junk S, Laufs U, Link A, Walenta K, Bohm M, Nickenig G. Intravenous transfusion of endothelial progenitor cells reduces neointima formation after vascular injury. Circ Res. 2003; 93: e17e24.[CrossRef][Medline] [Order article via Infotrieve]
53. Kaushal S, Amiel GE, Guleserian KJ, Shapira OM, Perry T, Sutherland FW, Rabkin E, Moran AM, Schoen FJ, Atala A, Soker S, Bischoff J, Mayer JE, Jr. Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. Nat Med. 2001; 7: 10351040.[CrossRef][Medline] [Order article via Infotrieve]
54. Griese DP, Ehsan A, Melo LG, Kong D, Zhang L, Mann MJ, Pratt RE, Mulligan RC, Dzau VJ. Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy. Circulation. 2003; 108: 27102715.
55. Hu Y, Davison F, Zhang Z, Xu Q. Endothelial replacement and angiogenesis in arteriosclerotic lesions of allografts are contributed by circulating progenitor cells. Circulation. 2003; 108: 31223127.
56. Silvestre JS, Gojova A, Brun V, Potteaux S, Esposito B, Duriez M, Clergue M, Le Ricousse-Roussanne S, Barateau V, Merval R, Groux H, Tobelem G, Levy B, Tedgui A, Mallat Z. Transplantation of bone marrow-derived mononuclear cells in ischemic apolipoprotein E-knockout mice accelerates atherosclerosis without altering plaque composition. Circulation. 2003; 108: 28392842.
57. Rossig L, Dimmeler S, Zeiher AM. Apoptosis in the vascular wall and atherosclerosis. Basic Res Cardiol. 2001; 96: 1122.[CrossRef][Medline] [Order article via Infotrieve]
58. Rauscher FM, Goldschmidt-Clermont PJ, Davis BH, Wang T, Gregg D, Ramaswami P, Pippen AM, Annex BH, Dong C, Taylor DA. Aging, progenitor cell exhaustion, and atherosclerosis. Circulation. 2003; 108: 457463.
59. Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyumi AA, Finkel T. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med. 2003; 348: 593600.
60. Vasa M, Fichtlscherer S, Aicher A, Adler K, Urbich C, Martin H, Zeiher AM, Dimmeler S. Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease. Circ Res. 2001; 89: e1e7.[CrossRef][Medline] [Order article via Infotrieve]
61. Tepper OM, Galiano RD, Capla JM, Kalka C, Gagne PJ, Jacobowitz GR, Levine JP, Gurtner GC. Human endothelial progenitor cells from type II diabetics exhibit impaired proliferation, adhesion, and incorporation into vascular structures. Circulation. 2002; 106: 27812786.
62. Loomans CJ, de Koning EJ, Staal FJ, Rookmaaker MB, Verseyden C, de Boer HC, Verhaar MC, Braam B, Rabelink TJ, van Zonneveld AJ. Endothelial progenitor cell dysfunction: a novel concept in the pathogenesis of vascular complications of type 1 diabetes. Diabetes. 2004; 53: 195199.
63. Tamarat R, Silvestre JS, Le Ricousse-Roussanne S, Barateau V, Lecomte-Raclet L, Clergue M, Duriez M, Tobelem G, Levy BI. Impairment in ischemia-induced neovascularization in diabetes: bone marrow mononuclear cell dysfunction and therapeutic potential of placenta growth factor treatment. Am J Pathol. 2004; 164: 457466.
64. Vasa M, Fichtlscherer S, Adler K, Aicher A, Martin H, Zeiher AM, Dimmeler S. Increase in circulating endothelial progenitor cells by statin therapy in patients with stable coronary artery disease. Circulation. 2001; 103: 28852890.
65. Laufs U, Werner N, Link A, Endres M, Wassmann S, Jurgens K, Miche E, Bohm M, Nickenig G. Physical Training Increases Endothelial Progenitor Cells, Inhibits Neointima Formation, and Enhances Angiogenesis. Circulation. 2004; 109: 220226.
66. Papayannopoulou T. Current mechanistic scenarios in hematopoietic stem/progenitor cell mobilization. Blood. 2004; 103: 15801585.
67. Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell. 2002; 109: 625637.[CrossRef][Medline] [Order article via Infotrieve]
68. Lee SH, Wolf PL, Escudero R, Deutsch R, Jamieson SW, Thistlethwaite PA. Early expression of angiogenesis factors in acute myocardial ischemia and infarction. N Engl J Med. 2000; 342: 626633.
69. Pillarisetti K, Gupta SK. Cloning and relative expression analysis of rat stromal cell derived factor-1 (SDF-1)1: SDF-1 alpha mRNA is selectively induced in rat model of myocardial infarction. Inflammation. 2001; 25: 293300.[CrossRef][Medline] [Order article via Infotrieve]
70. Shintani S, Murohara T, Ikeda H, Ueno T, Honma T, Katoh A, Sasaki K, Shimada T, Oike Y, Imaizumi T. Mobilization of endothelial progenitor cells in patients with acute myocardial infarction. Circulation. 2001; 103: 27762779.
71. Kalka C, Masuda H, Takahashi T, Gordon R, Tepper O, Gravereaux E, Pieczek A, Iwaguro H, Hayashi SI, Isner JM, Asahara T. Vascular endothelial growth factor (165) gene transfer augments circulating endothelial progenitor cells in human subjects. Circ Res. 2000; 86: 11981202.
72. Heeschen C, Aicher A, Lehmann R, Fichtlscherer S, Vasa M, Urbich C, Mildner-Rihm C, Martin H, Zeiher AM, Dimmeler S. Erythropoietin is a potent physiological stimulus for endothelial progenitor cell mobilization. Blood. 2003; 17: 17.
73. Bahlmann FH, De Groot K, Spandau JM, Landry AL, Hertel B, Duckert T, Boehm SM, Menne J, Haller H, Fliser D. Erythropoietin regulates endothelial progenitor cells. Blood. 2004; 103: 921926.
74. Moore MA, Hattori K, Heissig B, Shieh JH, Dias S, Crystal RG, Rafii S. Mobilization of endothelial and hematopoietic stem and progenitor cells by adenovector-mediated elevation of serum levels of SDF-1, VEGF, and angiopoietin-1. Ann N| Y Acad Sci. 2001; 938: 3645;discussion 4537.
75. Hattori K, Dias S, Heissig B, Hackett NR, Lyden D, Tateno M, Hicklin DJ, Zhu Z, Witte L, Crystal RG, Moore MA, Rafii S. Vascular endothelial growth factor and angiopoietin-1 stimulate postnatal hematopoiesis by recruitment of vasculogenic and hematopoietic stem cells. J Exp Med. 2001; 193: 10051014.
76. Dimmeler S, Aicher A, Vasa M, Mildner-Rihm C, Adler K, Tiemann M, Rutten H, Fichtlscherer S, Martin H, Zeiher AM. HMG-CoA reductase inhibitors (statins) increase endothelial progenitor cells via the PI 3-kinase/Akt pathway. J Clin Invest. 2001; 108: 391397.[CrossRef][Medline] [Order article via Infotrieve]
77. Iwakura A, Luedemann C, Shastry S, Hanley A, Kearney M, Aikawa R, Isner JM, Asahara T, Losordo DW. Estrogen-mediated, endothelial nitric oxide synthase-dependent mobilization of bone marrow-derived endothelial progenitor cells contributes to reendothelialization after arterial injury. Circulation. 2003; 108: 31153121.
78. Strehlow K, Werner N, Berweiler J, Link A, Dirnagl U, Priller J, Laufs K, Ghaeni L, Milosevic M, Bohm M, Nickenig G. Estrogen increases bone marrow-derived endothelial progenitor cell production and diminishes neointima formation. Circulation. 2003; 107: 30593065.
79. Kureishi Y, Luo Z, Shiojima I, Bialik A, Fulton D, Lefer DJ, Sessa WC, Walsh K. The HMG-CoA reductase inhibitor simvastatin activates the protein kinase Akt and promotes angiogenesis in normocholesterolemic animals. Nat Med. 2000; 6: 10041010.[CrossRef][Medline] [Order article via Infotrieve]
80. Vajkoczy P, Blum S, Lamparter M, Mailhammer R, Erber R, Engelhardt B, Vestweber D, Hatzopoulos AK. Multistep nature of microvascular recruitment of ex vivo-expanded embryonic endothelial progenitor cells during tumor angiogenesis. J Exp Med. 2003; 197: 17551765.
81. Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76: 301314.[CrossRef][Medline] [Order article via Infotrieve]
82. Carlos TM, Harlan JM. Leukocyte-endothelial adhesion molecules. Blood. 1994; 84: 20682101.
83. Muller WA. Leukocyte-endothelial cell interactions in the inflammatory response. Lab Invest. 2002; 82: 521533.[CrossRef][Medline] [Order article via Infotrieve]
84. Soligo D, Schiro R, Luksch R, Manara G, Quirici N, Parravicini C, Lambertenghi Deliliers G. Expression of integrins in human bone marrow. Br J Haematol. 1990; 76: 323332.[Medline] [Order article via Infotrieve]
85. Kollet O, Spiegel A, Peled A, Petit I, Byk T, Hershkoviz R, Guetta E, Barkai G, Nagler A, Lapidot T. Rapid and efficient homing of human CD34(+)CD38(-/low)CXCR4(+) stem and progenitor cells to the bone marrow and spleen of NOD/SCID and NOD/SCID/B2m(null) mice. Blood. 2001; 97: 32833291.
86. Peled A, Grabovsky V, Habler L, Sandbank J, Arenzana-Seisdedos F, Petit I, Ben-Hur H, Lapidot T, Alon R. The chemokine SDF-1 stimulates integrin-mediated arrest of CD34(+) cells on vascular endothelium under shear flow. J Clin Invest. 1999; 104: 11991211.[Medline] [Order article via Infotrieve]
87. Bowden RA, Ding ZM, Donnachie EM, Petersen TK, Michael LH, Ballantyne CM, Burns AR. Role of alpha4 integrin and VCAM-1 in CD18-independent neutrophil migration across mouse cardiac endothelium. Circ Res. 2002; 90: 562569.
88. Tasaka S, Richer SE, Mizgerd JP, Doerschuk CM. Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice. Am J Respir Crit Care Med. 2002; 166: 5360.
89. Scott LM, Priestley GV, Papayannopoulou T. Deletion of alpha4 integrins from adult hematopoietic cells reveals roles in homeostasis, regeneration, and homing. Mol Cell Biol. 2003; 23: 93499360.
90. Lapidot T. Mechanism of human stem cell migration and repopulation of NOD/SCID and B2mnull NOD/SCID mice. The role of SDF-1/CXCR4 interactions. Ann N Y Acad Sci. 2001; 938: 8395.[Medline] [Order article via Infotrieve]
91. Wright DE, Bowman EP, Wagers AJ, Butcher EC, Weissman IL. Hematopoietic stem cells are uniquely selective in their migratory response to chemokines. J Exp Med. 2002; 195: 11451154.
92. Kimura T, Boehmler AM, Seitz G, Kuci S, Wiesner T, Brinkmann V, Kanz L, Mohle R. The sphingosine 1-phosphate (S1P) receptor agonist FTY720 supports CXCR4-dependent migration and bone marrow homing of human CD34+ progenitor cells. Blood. 2004; 26: 26.
93. Adams GB, Chabner KT, Foxall RB, Weibrecht KW, Rodrigues NP, Dombkowski D, Fallon R, Poznansky MC, Scadden DT. Heterologous cells cooperate to augment stem cell migration, homing, and engraftment. Blood. 2003; 101: 4551.
94. Askari AT, Unzek S, Popovic ZB, Goldman CK, Forudi F, Kiedrowski M, Rovner A, Ellis SG, Thomas JD, DiCorleto PE, Topol EJ, Penn MS. Effect of stromal-cell-derived factor 1 on stem-cell homing and tissue regeneration in ischaemic cardiomyopathy. Lancet. 2003; 362: 697703.[CrossRef][Medline] [Order article via Infotrieve]
95. Britten MB, Abolmaali ND, Assmus B, Lehmann R, Honold J, Schmitt J, Vogl TJ, Martin H, Schachinger V, Dimmeler S, Zeiher AM. Infarct remodeling after intracoronary progenitor cell treatment in patients with acute myocardial infarction (TOPCARE-AMI): mechanistic insights from serial contrast-enhanced magnetic resonance imaging. Circulation. 2003; 108: 22122218.
96. Ferrara N, Carver-Moore K, Chen H, Dowd M, Lu L, OShea KS, Powell-Braxton L, Hillan KJ, Moore MW. Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene. Nature. 1996; 380: 439442.[CrossRef][Medline] [Order article via Infotrieve]
97. Fong GH, Rossant J, Gertsenstein M, Breitman ML. Role of the Flt-1 receptor tyrosine kinase in regulating the assembly of vascular endothelium. Nature. 1995; 376: 6670.[CrossRef][Medline] [Order article via Infotrieve]
98. Shalaby F, Rossant J, Yamaguchi TP, Gertsenstein M, Wu XF, Breitman ML, Schuh AC. Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature. 1995; 376: 6266.[CrossRef][Medline] [Order article via Infotrieve]
99. Guo Y, Chan R, Ramsey H, Li W, Xie X, Shelley WC, Martinez-Barbera JP, Bort B, Zaret K, Yoder M, Hromas R. The homeoprotein Hex is required for hemangioblast differentiation. Blood. 2003; 102: 24282435.
100. Zippo A, De Robertis A, Bardelli M, Galvagni F, Oliviero S. Identification of Flk-1-target genes in vasculogenesis: Pim-1 is required for endothelial and mural cell differentiation in vitro. Blood. 2004; 24: 24.
101. Bompais H, Chagraoui J, Canron X, Crisan M, Liu XH, Anjo A, Tolla-Le Port C, Leboeuf M, Charbord P, Bikfalvi A, Uzan G. Human endothelial cells derived from circulating progenitors display specific functional properties as compared to mature vessel wall endothelial cells. Blood. 2003; 20: 20.
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||||
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X. Li, Y. Han, W. Pang, C. Li, X. Xie, J. Y.-J. Shyy, and Y. Zhu AMP-Activated Protein Kinase Promotes the Differentiation of Endothelial Progenitor Cells Arterioscler Thromb Vasc Biol, October 1, 2008; 28(10): 1789 - 1795. [Abstract] [Full Text] [PDF] |
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J Avouac, F Juin, J Wipff, P O Couraud, G Chiocchia, A Kahan, C Boileau, G Uzan, and Y Allanore Circulating endothelial progenitor cells in systemic sclerosis: association with disease severity Ann Rheum Dis, October 1, 2008; 67(10): 1455 - 1460. [Abstract] [Full Text] [PDF] |
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C. G. Egan, F. Caporali, E. Garcia-Gonzalez, M. Galeazzi, and V. Sorrentino Endothelial progenitor cells and colony-forming units in rheumatoid arthritis: association with clinical characteristics Rheumatology, October 1, 2008; 47(10): 1484 - 1488. [Abstract] [Full Text] [PDF] |
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K. K. Hirschi, D. A. Ingram, and M. C. Yoder Assessing Identity, Phenotype, and Fate of Endothelial Progenitor Cells Arterioscler Thromb Vasc Biol, September 1, 2008; 28(9): 1584 - 1595. [Full Text] [PDF] |
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M. R. Schroeter, M. Leifheit, P. Sudholt, N.-M. Heida, C. Dellas, I. Rohm, F. Alves, M. Zientkowska, S. Rafail, M. Puls, et al. Leptin Enhances the Recruitment of Endothelial Progenitor Cells Into Neointimal Lesions After Vascular Injury by Promoting Integrin-Mediated Adhesion Circ. Res., August 29, 2008; 103(5): 536 - 544. [Abstract] [Full Text] [PDF] |
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A. A van der Klaauw, A. M Pereira, T. J Rabelink, E. P M Corssmit, A.-J. Zonneveld, H. Pijl, H. C de Boer, J. W A Smit, J. A Romijn, and E. J P de Koning Recombinant human GH replacement increases CD34+ cells and improves endothelial function in adults with GH deficiency Eur. J. Endocrinol., August 1, 2008; 159(2): 105 - 111. [Abstract] [Full Text] [PDF] |
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P. Salvatore, A. Casamassimi, L. Sommese, C. Fiorito, A. Ciccodicola, R. Rossiello, B. Avallone, V. Grimaldi, V. Costa, M. Rienzo, et al. Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells PNAS, July 8, 2008; 105(27): 9427 - 9432. [Abstract] [Full Text] [PDF] |
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R. P.W. Rouhl, R. J. van Oostenbrugge, J. Damoiseaux, J.-W. C. Tervaert, and J. Lodder Endothelial Progenitor Cell Research in Stroke: A Potential Shift in Pathophysiological and Therapeutical Concepts Stroke, July 1, 2008; 39(7): 2158 - 2165. [Abstract] [Full Text] [PDF] |
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W. Zhang, X.-H. Wang, S.-F. Chen, G.-P. Zhang, N. Lu, R.-M. Hu, and H.-M. Jin Biphasic response of endothelial progenitor cell proliferation induced by high glucose and its relationship with reactive oxygen species J. Endocrinol., June 1, 2008; 197(3): 463 - 470. [Abstract] [Full Text] [PDF] |
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N. Smart and P. R. Riley The Stem Cell Movement Circ. Res., May 23, 2008; 102(10): 1155 - 1168. [Abstract] [Full Text] [PDF] |
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P. van der Harst, D. J. van Veldhuisen, and N. J. Samani Expanding the Concept of Telomere Dysfunction in Cardiovascular Disease Arterioscler Thromb Vasc Biol, May 1, 2008; 28(5): 807 - 808. [Full Text] [PDF] |
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G.-R. Dou, Y.-C. Wang, X.-B. Hu, L.-H. Hou, C.-M. Wang, J.-F. Xu, Y.-S. Wang, Y.-M. Liang, L.-B. Yao, A.-G. Yang, et al. RBP-J, the transcription factor downstream of Notch receptors, is essential for the maintenance of vascular homeostasis in adult mice FASEB J, May 1, 2008; 22(5): 1606 - 1617. [Abstract] [Full Text] [PDF] |
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G. P. Fadini, S. de Kreutzenberg, M. Albiero, A. Coracina, E. Pagnin, I. Baesso, A. Cignarella, C. Bolego, M. Plebani, G. B. Nardelli, et al. Gender Differences in Endothelial Progenitor Cells and Cardiovascular Risk Profile: The Role of Female Estrogens Arterioscler Thromb Vasc Biol, May 1, 2008; 28(5): 997 - 1004. [Abstract] [Full Text] [PDF] |
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R. Vanholder, U. Baurmeister, P. Brunet, G. Cohen, G. Glorieux, J. Jankowski, and for the European Uremic Toxin Work Group A Bench to Bedside View of Uremic Toxins J. Am. Soc. Nephrol., May 1, 2008; 19(5): 863 - 870. [Abstract] [Full Text] [PDF] |
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V. W.M. van Hinsbergh and P. Koolwijk Endothelial sprouting and angiogenesis: matrix metalloproteinases in the lead Cardiovasc Res, May 1, 2008; 78(2): 203 - 212. [Abstract] [Full Text] [PDF] |
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J. Tongers, J.-M. Knapp, M. Korf, T. Kempf, A. Limbourg, F. P. Limbourg, Z. Li, D. Fraccarollo, J. Bauersachs, X. Han, et al. Haeme oxygenase promotes progenitor cell mobilization, neovascularization, and functional recovery after critical hindlimb ischaemia in mice Cardiovasc Res, May 1, 2008; 78(2): 294 - 300. [Abstract] [Full Text] [PDF] |
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G. Spinetti, N. Kraenkel, C. Emanueli, and P. Madeddu Diabetes and vessel wall remodelling: from mechanistic insights to regenerative therapies Cardiovasc Res, May 1, 2008; 78(2): 265 - 273. [Abstract] [Full Text] [PDF] |
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S. Jelic, M. Padeletti, S. M. Kawut, C. Higgins, S. M. Canfield, D. Onat, P. C. Colombo, R. C. Basner, P. Factor, and T. H. LeJemtel Inflammation, Oxidative Stress, and Repair Capacity of the Vascular Endothelium in Obstructive Sleep Apnea Circulation, April 29, 2008; 117(17): 2270 - 2278. [Abstract] [Full Text] [PDF] |
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E. Chavakis, G. Carmona, C. Urbich, S. Gottig, R. Henschler, J. M. Penninger, A. M. Zeiher, T. Chavakis, and S. Dimmeler Phosphatidylinositol-3-Kinase-{gamma} Is Integral to Homing Functions of Progenitor Cells Circ. Res., April 25, 2008; 102(8): 942 - 949. [Abstract] [Full Text] [PDF] |
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H. E. Thomas, R. Redgrave, M. S. Cunnington, P. Avery, B. D. Keavney, and H. M. Arthur Circulating Endothelial Progenitor Cells Exhibit Diurnal Variation Arterioscler Thromb Vasc Biol, March 1, 2008; 28(3): e21 - e22. [Full Text] [PDF] |
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D. F. Alvarez, L. Huang, J. A. King, M. K. ElZarrad, M. C. Yoder, and T. Stevens Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L419 - L430. [Abstract] [Full Text] [PDF] |
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K. Asosingh, M. A. Aldred, A. Vasanji, J. Drazba, J. Sharp, C. Farver, S. A.A. Comhair, W. Xu, L. Licina, L. Huang, et al. Circulating Angiogenic Precursors in Idiopathic Pulmonary Arterial Hypertension Am. J. Pathol., March 1, 2008; 172(3): 615 - 627. [Abstract] [Full Text] [PDF] |
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G. Carmona, E. Chavakis, U. Koehl, A. M. Zeiher, and S. Dimmeler Activation of Epac stimulates integrin-dependent homing of progenitor cells Blood, March 1, 2008; 111(5): 2640 - 2646. [Abstract] [Full Text] [PDF] |
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A. Whittaker, J. S. Moore, M. Vasa-Nicotera, S. Stevens, and N. J. Samani Evidence for genetic regulation of endothelial progenitor cells and their role as biological markers of atherosclerotic susceptibility Eur. Heart J., February 1, 2008; 29(3): 332 - 338. [Abstract] [Full Text] [PDF] |
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S. Dimmeler, J. Burchfield, and A. M. Zeiher Cell-Based Therapy of Myocardial Infarction Arterioscler Thromb Vasc Biol, February 1, 2008; 28(2): 208 - 216. [Abstract] [Full Text] [PDF] |
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M. L. Balestrieri, C. Schiano, F. Felice, A. Casamassimi, A. Balestrieri, L. Milone, L. Servillo, and C. Napoli Effect of Low Doses of Red Wine and Pure Resveratrol on Circulating Endothelial Progenitor Cells J. Biochem., February 1, 2008; 143(2): 179 - 186. [Abstract] [Full Text] [PDF] |
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S. Nogueras, A. Merino, R. Ojeda, J. Carracedo, M. Rodriguez, A. Martin-Malo, R. Ramirez, and P. Aljama Coupling of endothelial injury and repair: an analysis using an in vivo experimental model Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H708 - H713. [Abstract] [Full Text] [PDF] |
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J. Zoll, V. Fontaine, P. Gourdy, V. Barateau, J. Vilar, A. Leroyer, I. Lopes-Kam, Z. Mallat, J.-F. Arnal, P. Henry, et al. Role of human smooth muscle cell progenitors in atherosclerotic plaque development and composition Cardiovasc Res, February 1, 2008; 77(3): 471 - 480. [Abstract] [Full Text] [PDF] |
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D. Yang, M. Koupenova, D. J. McCrann, K. J. Kopeikina, H. M. Kagan, B. M. Schreiber, and K. Ravid The A2b adenosine receptor protects against vascular injury PNAS, January 15, 2008; 105(2): 792 - 796. [Abstract] [Full Text] [PDF] |
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K. Stellos, H. Langer, K. Daub, T. Schoenberger, A. Gauss, T. Geisler, B. Bigalke, I. Mueller, M. Schumm, I. Schaefer, et al. Platelet-Derived Stromal Cell-Derived Factor-1 Regulates Adhesion and Promotes Differentiation of Human CD34+ Cells to Endothelial Progenitor Cells Circulation, January 15, 2008; 117(2): 206 - 215. [Abstract] [Full Text] [PDF] |
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P. Madeddu, N. Kraenkel, L. S. Barcelos, M. Siragusa, P. Campagnolo, A. Oikawa, A. Caporali, A. Herman, O. Azzolino, L. Barberis, et al. Phosphoinositide 3-Kinase {gamma} Gene Knockout Impairs Postischemic Neovascularization and Endothelial Progenitor Cell Functions Arterioscler Thromb Vasc Biol, January 1, 2008; 28(1): 68 - 76. [Abstract] [Full Text] [PDF] |
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M. Mints, M. Jansson, B. Sadeghi, M. Westgren, M. Uzunel, M. Hassan, and J. Palmblad Endometrial endothelial cells are derived from donor stem cells in a bone marrow transplant recipient Hum. Reprod., January 1, 2008; 23(1): 139 - 143. [Abstract] [Full Text] [PDF] |
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W Wojakowski, M Kucia, M Kazmierski, M Z Ratajczak, and M Tendera Circulating progenitor cells in stable coronary heart disease and acute coronary syndromes: relevant reparatory mechanism? Heart, January 1, 2008; 94(1): 27 - 33. [Abstract] [Full Text] [PDF] |
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M. T. Kearney, E. R. Duncan, M. Kahn, and S. B. Wheatcroft Insulin resistance and endothelial cell dysfunction: studies in mammalian models Exp Physiol, January 1, 2008; 93(1): 158 - 163. [Abstract] [Full Text] [PDF] |
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M. Koyanagi, P. Bushoven, M. Iwasaki, C. Urbich, A. M. Zeiher, and S. Dimmeler Notch Signaling Contributes to the Expression of Cardiac Markers in Human Circulating Progenitor Cells Circ. Res., November 26, 2007; 101(11): 1139 - 1145. [Abstract] [Full Text] [PDF] |
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E. J. Suuronen, J. Price, J. P. Veinot, K. Ascah, V. Kapila, X.-W. Guo, S. Wong, T. G. Mesana, and M. Ruel Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac function. J. Thorac. Cardiovasc. Surg., November 1, 2007; 134(5): 1249 - 1258. [Abstract] [Full Text] [PDF] |
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M. Y.K. Lee, H.-F. Tse, C.-W. Siu, S.-G. Zhu, R. Y.K. Man, and P. M. Vanhoutte Genomic Changes in Regenerated Porcine Coronary Arterial Endothelial Cells Arterioscler Thromb Vasc Biol, November 1, 2007; 27(11): 2443 - 2449. [Abstract] [Full Text] [PDF] |
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J. Tongers and D. W. Losordo Frontiers in Nephrology: The Evolving Therapeutic Applications of Endothelial Progenitor Cells J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2843 - 2852. [Abstract] [Full Text] [PDF] |
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J. M. L. Ebos, C. R. Lee, J. G. Christensen, A. J. Mutsaers, and R. S. Kerbel Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy PNAS, October 23, 2007; 104(43): 17069 - 17074. [Abstract] [Full Text] [PDF] |
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J. Hur, H.-M. Yang, C.-H. Yoon, C.-S. Lee, K.-W. Park, J.-H. Kim, T.-Y. Kim, J.-Y. Kim, H.-J. Kang, I.-H. Chae, et al. Identification of a Novel Role of T Cells in Postnatal Vasculogenesis: Characterization of Endothelial Progenitor Cell Colonies Circulation, October 9, 2007; 116(15): 1671 - 1682. [Abstract] [Full Text] [PDF] |
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Z. Li, J. C. Wu, A. Y. Sheikh, D. Kraft, F. Cao, X. Xie, M. Patel, S. S. Gambhir, R. C. Robbins, J. P. Cooke, et al. Differentiation, Survival, and Function of Embryonic Stem Cell Derived Endothelial Cells for Ischemic Heart Disease Circulation, September 11, 2007; 116(11_suppl): I-46 - I-54. [Abstract] [Full Text] [PDF] |
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B. Mees, S. Wagner, E. Ninci, S. Tribulova, S. Martin, R. van Haperen, S. Kostin, M. Heil, R. de Crom, and W. Schaper Endothelial Nitric Oxide Synthase Activity Is Essential for Vasodilation During Blood Flow Recovery but not for Arteriogenesis Arterioscler Thromb Vasc Biol, September 1, 2007; 27(9): 1926 - 1933. [Abstract] [Full Text] [PDF] |
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H. Shmilovich, V. Deutsch, A. Roth, H. Miller, G. Keren, and J. George Circulating endothelial progenitor cells in patients with cardiac syndrome X Heart, September 1, 2007; 93(9): 1071 - 1076. [Abstract] [Full Text] [PDF] |
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W. Wojakowski, M. Kazmierski, B. Korzeniowska, and M. Tendera Link between erythropoietin release and mobilization of endothelial progenitor cells in acute myocardial infarction Eur. Heart J., August 1, 2007; 28(15): 1785 - 1786. [Full Text] [PDF] |
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H.-F. Tse, C.-W. Siu, S.-G. Zhu, L. Songyan, Q.-Y. Zhang, W.-H. Lai, Y.-L. Kwong, J. Nicholls, and C.-P. Lau Paracrine effects of direct intramyocardial implantation of bone marrow derived cells to enhance neovascularization in chronic ischaemic myocardium Eur J Heart Fail, August 1, 2007; 9(8): 747 - 753. [Abstract] [Full Text] [PDF] |
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S. Levenberg, J. Zoldan, Y. Basevitch, and R. Langer Endothelial potential of human embryonic stem cells Blood, August 1, 2007; 110(3): 806 - 814. [Abstract] [Full Text] [PDF] |
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P. E Westerweel, R. K M A C Luijten, I. E Hoefer, H. A Koomans, R. H W M Derksen, and M. C Verhaar Haematopoietic and endothelial progenitor cells are deficient in quiescent systemic lupus erythematosus Ann Rheum Dis, July 1, 2007; 66(7): 865 - 870. [Abstract] [Full Text] [PDF] |
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M. Nagano, T. Yamashita, H. Hamada, K. Ohneda, K.-i. Kimura, T. Nakagawa, M. Shibuya, H. Yoshikawa, and O. Ohneda Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood Blood, July 1, 2007; 110(1): 151 - 160. [Abstract] [Full Text] [PDF] |
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K.-H. Chang, T. Chan-Ling, E. L. McFarland, A. Afzal, H. Pan, L. C. Baxter, L. C. Shaw, S. Caballero, N. Sengupta, S. L. Calzi, et al. IGF binding protein-3 regulates hematopoietic stem cell and endothelial precursor cell function during vascular development PNAS, June 19, 2007; 104(25): 10595 - 10600. [Abstract] [Full Text] [PDF] |
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H.-K. Oh, J.-M. Ha, E. O, B. H. Lee, S. K. Lee, B.-S. Shim, Y.-K. Hong, and Y. A. Joe Tumor Angiogenesis Promoted by Ex vivo Differentiated Endothelial Progenitor Cells Is Effectively Inhibited by an Angiogenesis Inhibitor, TK1-2 Cancer Res., May 15, 2007; 67(10): 4851 - 4859. [Abstract] [Full Text] [PDF] |
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E. W. Raines Inflamed endothelium: an EPC adhesion kit Blood, May 15, 2007; 109(10): 4118 - 4118. [Full Text] [PDF] |
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R. R. Langley and I. J. Fidler Tumor Cell-Organ Microenvironment Interactions in the Pathogenesis of Cancer Metastasis Endocr. Rev., May 1, 2007; 28(3): 297 - 321. [Abstract] [Full Text] [PDF] |
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G. P. Fadini, S. Sartore, C. Agostini, and A. Avogaro Significance of Endothelial Progenitor Cells in Subjects With Diabetes Diabetes Care, May 1, 2007; 30(5): 1305 - 1313. [Full Text] [PDF] |
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T. Thum, D. Fraccarollo, S. Thum, M. Schultheiss, A. Daiber, P. Wenzel, T. Munzel, G. Ertl, and J. Bauersachs Differential Effects of Organic Nitrates on Endothelial Progenitor Cells Are Determined by Oxidative Stress Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 748 - 754. [Abstract] [Full Text] [PDF] |
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C. Murphy, G. S. Kanaganayagam, B. Jiang, P. J. Chowienczyk, R. Zbinden, M. Saha, S. Rahman, A. M. Shah, M. S. Marber, and M. T. Kearney Vascular Dysfunction and Reduced Circulating Endothelial Progenitor Cells in Young Healthy UK South Asian Men Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 936 - 942. [Abstract] [Full Text] [PDF] |
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