Integrative Physiology |
From the Departments of Cardiology and Angiology (D.H.-K., A. Hilfiker, M.F., K.K., A. Schaefer, B.S., A. Hillmer, Edith Podewski, Eva Podewski, K.C.W., H.D.), Anatomy (A. Schmiedl), and Nephrology (J.-K.P.), Medical School Hannover, Germany; Institute for Cardiovascular Physiology (Z.D.), University of Düsseldorf, Germany; Department of Genetics, Biology, and Biochemistry (V.P.), University of Turin, Italy; Department of Medicine (M.D.S.), Baylor College of Medicine, Houston, Tex; Department of Pathophysiology (R.S.), Universitätsklinikum, Essen, Germany.
Correspondence to Helmut Drexler, MD, Abt Kardiologie und Angiologie, Medizinische Hochschule Hannover, Carl-Neuberg Str 1, 30625 Hannover, Germany. E-mail drexler.helmut{at}mh-hannover.de
| Abstract |
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Key Words: mouse signal transduction angiogenesis ischemia heart failure
| Introduction |
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To elucidate the potential role of STAT3 in cardiac muscle and, in particular, for cardiac protection against physiological and pathophysiological stress, we created mice with a cardiomyocyte-restricted STAT3 deletion.
| Materials and Methods |
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-myosin heavy chain (
MHC)-promoter/Cre-recombinase transgenic mice8 were bred to generate mice with a cardiomyocyte-restricted STAT3 deletion (online data supplement available at http://circres.ahajournals.org). Only male mice were studied. All animal studies were in compliance with the Guide for the Care and Use of Laboratory Animals as published by the US National Institutes of Health and approved by our local institutional review boards.
Animal Experiments
I/R injury and MI were performed in 12±2-week-old mice as described9,10 (online supplement). Control mice underwent a thoracotomy only (sham).
Transthoracic Echocardiography and Hemodynamics
Left ventricle (LV) hemodynamics were measured as described.10 The right jugular vein was cannulated for infusion of dobutamine (4 µg/kg per min up to 40 µg/kg per min; online supplement). Echocardiography was performed in sedated mice (ketamine, 100 mg/kg IP and xylazine, 1.25 mg/kg IP) as described11 (online supplement).
Langendorff Preparations
Langendorff preparations were performed as described.12
Metabolic Assay
Measurement of heart tissue levels of lactate, ATP, ADP, and AMP with high-performance liquid chromatography were described13,14 (online supplement).
Quantification of I/R and MI Injury
After I/R, area at risk and early infarct size were assessed by Evans blue and 2,3,5-triphenyltetrazolium chloride staining.10 After I/R (7 days), respectively after permanent coronary ligation, MI sizes were determined in myocardial sections fixed in situ and stained with hematoxylin/eosin (H&E).10
Treatment of Mice With an Agonistic Anti-Fas Antibody
Monoclonal anti-mouse Fas IgG (10 µg Jo2; Pharmingen) or antitrinitrophenol control IgG (Pharmingen) was injected intraperitoneally. After 24 hours, cryosections were prepared from the LV.15
Histological Analyses, Immunostaining, and Electron Microscopy
LV tissue slices were stained with Sirius red F3BA, van Gieson, or H&E, as described.10 Interstitial collagen volume fraction was determined in picro-Sirius red F3BA-stained sections (online supplement).10 Apoptotic nuclei were detected by TUNEL, and apoptotic morphology of nuclei was confirmed by counterstaining with Hoechst 33258 (Sigma; online supplement).15 Immunohistochemistry was performed with antibodies recognizing the C terminus of the STAT3 protein (Cell Signaling Technology), sarcomeric
-actinin (Sigma), platelet-endothelial cell adhesion molecule-1 (PECAM-1), tissue inhibitor of metalloproteinase-1 (TIMP1), or thrombospondin-1 (TSP1; Santa Cruz Biotechnology). Additional LV tissue samples were analyzed by electron microscopy.16
LV capillary density was determined either as the ratio of PECAM-1-positive cells to total nuclei (hematoxylin or Hoechst stain) or as capillary to cardiomyocytes ratio in van Gieson or PECAM-1/wheat germ agglutinin (WGA; Vector Laboratories)-stained LV sections (online supplement). Density of coronary resistance vessels (Ø 25 to 150 µm) was determined on H&E-stained LV sections (online supplement).
DNA Laddering
Genomic DNA was isolated from LV tissue and separated on a 1.4% agarose gel (35 µg per lane).
RT-PCR, Northern, and Immunoblot Analyses
Northern blotting and RT-PCR were performed according to standard procedures17 using primers, cDNA, and oligonucleotide probes described in the online supplement. Antibodies against vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1
(HIF1
), basic fibroblast growth factor (bFGF), TIMP1, TSP1 (Santa Cruz Biotechnology), IL-15 (R&D), STAT3, and tyrosine phosphorylation (phospho-Tyr)-STAT3 (Cell Signaling Technology) were used for immunoblots.
Cell Culture
Cardiomyocytes from 3- to 4-month-old wild-type (WT) and knockout (KO) mice were isolated using 0.04% collagenase (Worthington) and plated in DMEM/medium 199 (M199)/5% FCS/10% horse serum on culture dishes for 24 hours, and then the medium was changed to serum-free DMEM/M199 for an additional 12 hours. Cell proliferation was assessed in mouse embryonic fibroblasts (MEFs) and in primary cultures of adult mouse lung endothelial cells (MLECs) by determining [3H]-thymidine incorporation (online supplement).
cDNA Expression Microarray Analysis
Total LV RNA was isolated from 3- to 4-month-old WT and KO mice, reverse transcribed into Cy3-dCTP- and Cy5-dCTP-labeled cDNA probes, and hybridized to Parallel Identification and Quantification of RNA Immuno/Onco cDNA microarrays, containing cDNA sequences from 648 mouse genes (Biozym Diagnostik). To minimize intragroup variation, cDNA probes from 5 hearts per genotype were pooled.
Statistical Analyses
Data are presented as mean±SD. Differences between groups were analyzed by Mann-Whitney test, Student t test, or log-rank test, as appropriate. A 2-tailed P<0.05 was considered statistically significant.
| Results |
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MHC promoter (
MHC-Cretg/), which directs transgene expression to cardiomyocytes (Figure 1). The efficiency and specificity of
MHC-Cre-mediated deletion of floxed genes have been demonstrated previously.8,18
MHC-Cretg/; STAT3flox/flox (KO), STAT3flox/flox (WT), and heterozygous mice (
MHC-Cretg/; STAT3flox/+) were born according to Mendelian inheritance ratios, survived into adulthood, and were fertile. PCR analyses of genomic DNA confirmed complete Cre/loxP recombination in cardiomyocytes isolated from 3-month-old KO mice (Figure 1A). Shortly after birth, STAT3 protein levels were reduced by
40% in isolated cardiomyocytes from KO compared with WT mice; LV STAT3 protein levels then gradually decreased in KO mice, resulting in a virtually complete, cardiomyocyte-restricted STAT3 deletion at the age of 3 months (Figure 1B). The faint band of STAT3 protein observed in LV extracts from KO mice represents expression in noncardiomyocytes, as shown by immunohistochemistry (Figure 1C through 1E). Heterozygotes, expressed slightly lower amounts of STAT3 protein compared with WT mice but were not significantly different from WT in terms of survival and cardiac performance in all experiments (data not shown).
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Reduced Myocardial Capillarization and Enhanced Fibrosis in STAT3 KO Mice
LV to body weight ratios and cardiac dimensions (Table), cardiomyocyte ultrastructure (Figure 2A), and expression levels of
MHC, ßMHC, skeletal muscle (SkM)
-actin, sarco(endo)plasmic reticulum Ca2+-ATPase-2a (SERCA2a), and atrial natriuretic peptide (ANP) mRNA (Figure 2B) did not differ in 3- to 4-month-old WT and KO mice. However, STAT3 deletion in cardiomyocytes resulted in reduced LV capillarization, as shown by anti-PECAM-1/WGA immunohistochemistry (Figure 2C), and confirmed in van Gieson-stained sections (capillary to cardiomyocyte ratio 1.6±0.2 versus 1.0±0.2 in WT and KO; n=6 each; P<0.01). Reduced myocardial capillary density in KO mice was slowly evolving after birth and was significant at the age of 8 to 12 weeks (Figure 2D), indicating that STAT3 in cardiomyocytes is required postnatally for myocardial capillary growth. Average density of coronary resistance vessels (Ø 25 to 150 µm) was similar in WT and KO mice (online supplement). Basal and peak coronary flow, known to be regulated primarily by coronary resistance vessels rather than capillaries,19 were not altered in Langendorff-perfused KO hearts (data not shown). However, KO hearts displayed lower heart rates (KO 506±17 versus WT 565±17 bpm; P<0.01) and lower dP/dt(max) (KO 6662±1075 versus WT 8033±509 mm Hg per sec; P=0.08) after administration of dobutamine, a ß-adrenergic agonist (n=6 each; online supplement). The lactate concentration was significantly increased (KO 5.6±0.7 versus WT 3.4±1.3 µmol/g; P<0.02) and the ratio of high-energy phosphate ATP to ADP tended to be lower (KO 0.75±0.15 versus WT 0.98±0.22, P=0.06) in KO versus WT LVs after dobutamine infusion (KO n=4; WT n=5; online supplement). Interstitial collagen volume fraction was increased moderately in 3- to 4-month-old KO mice (Figure 2E; WT 0.15±0.08% versus KO 0.33±0.10%; n=5 each; P<0.05) and strongly augmented in 6-month-old KO mice (0.18±0.05% versus 0.82±0.20% in WT and KO; n=4 each; P<0.01).
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Reduced capillary density and enhanced interstitial fibrosis in KO suggested that STAT3 controls expression of downstream genes in cardiomyocytes that may be involved in regulation of myocardial capillarization or fibrosis. However, protein expression levels of HIF-1
, VEGF, and bFGF (genes known to be critically involved in angiogenesis) were not different in LVs from KO and WT (Figure 3A). To identify additional candidate genes, cDNA probes derived from LVs of 3- to 4-month-old WT and KO were hybridized to custom-made cDNA microarrays containing 648 genes, including genes putatively involved in angiogenesis or control of interstitial matrix composition (online supplement). Genes differing in their expression levels >1.8-fold were considered differently expressed (Figure 3B). For some of these genes, differential expression was confirmed independently by RT-PCR, Northern blotting, immunoblotting, or immunohistochemistry in at least n=4 mice of each genotype (Figure 3A through 3D; data not shown). Overall, the cDNA microarray analyses revealed a shift in gene expression pattern consistent with a profibrotic and antiangiogenic state, with increased expression levels of collagen-1
(COL1A1), COL8A1, osteopontin (OPN), biglycan, tenascin-C (TNC), plasminogen activator inhibitor-1 (PAI-1), connective tissue growth factor (CTGF), TSP1, and TIMP12024 (Figure 3B), whereas expression of matrix metalloproteinase type 2 (MMP-2) and MMP-9 was unchanged (data not shown). Several of these factors, including CTGF, TSP1, and TIMP1, are known to be potent antiangiogenic factors.2527 For example, CTGF and TSP1 have been shown previously to inhibit VEGF angiogenic activity.25,26 Immunohistochemistry demonstrated enhanced TIMP1 and TSP1 levels associated with the extracellular matrix in KO (Figure 3C and 3D). Furthermore, isolated cardiomyocytes from KO mice displayed increased protein abundance of TIMP1 and TSP1 (data not shown). Because the antiangiogenic activity of TSP1 depends, in part, on Fas-mediated endothelial cell apoptosis,28 we injected an agonistic anti-Fas antibody into KO and WT mice (n=5 each). As determined by TUNEL/Hoechst-staining and anti-PECAM-1 immunohistochemistry, stimulation of the Fas receptor promoted significantly more endothelial cell apoptosis in KO LVs (0.033
versus 0.013
; P<0.05).
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The presence of paracrine factors released by KO cardiomyocytes was confirmed by the finding that KO cardiomyocytes supernatant induced a higher proliferation rate in fibroblasts (MEFs) and a lower proliferation rate in endothelial cells (MLECs) compared with WT cardiomyocytes supernatant (Figure 3E).
Cardiomyopathy With Massive Fibrosis and High Mortality During Long-Term Follow-Up in STAT3 KO Mice
Beyond 9 months, KO mice displayed an enhanced mortality rate (Figure 4A). Decreased survival in KO mice was associated with heart failure symptoms such as labored breathing and generalized edema (data not shown). At 12 months, the hearts of KO mice were dilated (Figure 4B and 4F) with massive interstitial fibrosis (Figure 4C) and increased rate of apoptosis (mainly nonmyocytes because most TUNEL-positive nuclei were located in cells negative for sarcomeric
-actinin; data not shown; Figure 4D), along with substantially enhanced expression levels of ANP (+297%; P<0.01), SkM
-actin (+85%; P<0.05), TIMP1 (+900%; P<0.01), TSP1 (+317%; P<0.01), and the proapoptotic Bcl-2 family member Bcl-2/adenovirus EIB 19kD-interacting protein 3 (BNIP3;29 +78%; P<0.05), whereas ßMHC and SERCA2a expression were not significantly altered compared with WT (Figure 4E). Furthermore, KO mice showed impaired cardiac contractile function (Figure 4F). Heart rate was similar in both genotypes (data not shown).
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Increased Susceptibility to I/R Injury in STAT3 KO Mice
In WT mice, ischemia for 1 hour, followed by reperfusion for 1 hour or 24 hours, induced rapid phospho-Tyr of STAT3 in the remote (nonischemic) and reperfused (I/R) LV. In contrast, only faint STAT3-phosphorylation was observed in KO mice, presumably because of STAT3 activation in noncardiomyocytes (Figure 5A). The area at risk during left anterior descending (LAD) occlusion was similar in WT and KO mice (Figure 5B). However, infarct sizes after 24 hours of reperfusion were significantly greater in KO LVs (Figure 5B), and this was associated with enhanced apoptosis in cardiomyocytes in the reperfused area (Figure 5C and 5D; immunohistochemistry for caspase-3 in online supplement). No increases in apoptotic nuclei were observed in the remote myocardium after I/R (24 hours) in either genotype (data not shown). After I/R (24 hours), both genotypes displayed similar increases in transcript levels of VEGF (452±240% versus 451±102% in WT and KO), Bcl-2 (349±130% versus 447±128% in WT and KO), and Bcl-X (354±92% versus 396±160% in WT and KO). In contrast, BNIP3 induction in KO was more pronounced (2.2±0.9-fold versus WT; P<0.05; Figure 5D), whereas induction of cardioprotective heat shock protein 70 (HSP70)30 was lower (KO 333±86% versus WT 648±13%; P<0.05; Figure 5D). Moreover, fractional shortening after I/R (7 days) was significantly impaired in KO mice (Table).
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Reduced Cardiac Function and Increased Mortality After MI in STAT3 KO Mice
Permanent LAD ligation caused a striking increase in mortality in KO mice; After 6 months, 21 of 21 KO mice (100%) but only 6 of 19 WT mice (32%) had died (Kaplan-Meier curve; online supplement). All sham-operated KO and WT mice (n=6 each) survived 6 months. Two weeks after MI, KO mice showed larger infarct sizes (WT, n=6 37±9% versus KO, n=7 51±12%; P<0.05), a more pronounced deterioration in LV systolic function (Table), an attenuated increase in cardiomyocyte cross-sectional area (Table), and more interstitial fibrosis in the remote LV (KO 0.80±0.24% versus WT 0.36±0.16%; P<0.05).
| Discussion |
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Previous studies have indicated that overexpression or activation of STAT3 in cardiomyocytes enhances VEGF expression, which, in turn, promotes myocardial capillary formation,33 whereas cardiomyocyte-specific deletion of VEGF reduces coronary microvascularization,34 suggesting that VEGF may be an important target gene mediating proangiogenic effects of STAT3. However, VEGF expression was not reduced in KO hearts, indicating that STAT3-dependent postnatal capillary growth does not involve VEGF expression regulation. Moreover, I/R-induced VEGF upregulation was similar in WT and KO mice, indicating that STAT3 in cardiomyocytes is not required for VEGF upregulation after I/R injury. Remarkably, KO mice displayed an upregulation of CTGF, an endogenous inhibitor of VEGF activity,26 and increased expression levels of TSP1, which suppresses capillary formation by inhibiting VEGF release and inducing endothelial cell apoptosis.25,28,35 Moreover, expression of the potent antiangiogenic factor TIMP127 was augmented in KO hearts.
Several antiangiogenic factors are also involved in the formation of interstitial matrix (eg, CTGF, TSP1, TIMP1, OPN, TNC, and PAI-1).2024 All of these genes are upregulated in KO mice, which is consistent with the profibrotic state in these animals. Vice versa, there is evidence that enhanced interstitial matrix formation inhibits angiogenesis,36 supporting the concept that the antiangiogenic and profibrotic cardiac phenotypes of KO mice are mediated, at least in part, by overlapping paracrine mechanisms. A potential cardiomyocyte-dependent paracrine mechanism promoting fibrosis and inhibiting angiogenesis in KO mice is supported by the observation that supernatant of cultured KO cardiomyocytes inhibited proliferation in endothelial cells and enhanced fibroblast proliferation. Although the cell type(s) expressing antiangiogenic or profibrotic factors and the precise molecular pathways controlling capillarization and interstitial matrix composition in KO mice have not been addressed in this study, our in vitro data suggest that STAT3 controls release of paracrine factors by cardiomyocytes that directly affect the phenotype of endothelial cells and fibroblasts. In addition, our microarray analyses have uncovered several candidate mechanisms whereby STAT3 ablation in cardiomyocytes inhibits capillary formation and promotes interstitial fibrosis in the myocardium. It is considerable that the STAT3 impact on angiogenesis and interstitial matrix is not related to altered expression of a single gene, but rather to differential expression of an array of genes involved in the fine tuning of interstitial matrix deposition and angiogenesis.
There could be alternative explanations for the enhanced fibrosis in KO myocardium, such as replacement fibrosis caused by myocyte loss as a result of decreased myocardial blood supply or oxidative stress secondary to tissue hypoxia/ischemia driving fibrosis. Although we did not observe enhanced apoptosis or upregulation of hypoxia markers such as HIF1
or BNIP3 in young KO mice under normal conditions, impaired cardiac perfusion and metabolism became obvious during dobutamine stress and resulted in increased lactate production. In addition, a recent report by Jacoby et al37 suggested that the STAT3 pathway is required to suppress inflammation, and thus reactive fibrosis may arise in response to low levels of inflammation. In this context, there is evidence that STAT3 can act as a direct transcriptional inhibitor for nuclear factor
B-mediated gene expression.38
Previous studies using cell-specific overexpression of STAT3 have identified several target genes for STAT3, including VEGF and the Bcl-2 family of antiapoptotic proteins.39 However, endogenous STAT3 does not appear to be required for expression of VEGF and Bcl-2 family members (Bcl-2, Bcl-X) in the heart. In this regard, it should be noted that the expression levels of the proapoptotic Bcl-2 family member BNIP3 were not altered in young KO mice but were significantly increased only in aged KO mice (presenting with heart failure) or in KO mice after I/R injury. Thus, it appears that the upregulation of BNIP3 is secondary, possibly related to myocardial hypoxia, resulting from reduced capillary density and increased interstitial fibrosis.29
Development of severe cardiac fibrosis in aging KO mice was associated with impaired cardiac function, increased apoptosis, ventricular remodeling, and heart failure with generalized edema and enhanced mortality (ie, typical features noted in patients with dilated cardiomyopathy and end-stage heart failure). We have shown previously that STAT3 protein abundance and activation are markedly reduced in failing human hearts.6 Therefore, it is conceivable that reduced STAT3 expression and activation in patients with heart failure adversely affect fundamental cardioprotective mechanisms (in analogy to what is observed in our KO mice), thereby contributing to the progression of heart failure.
As revealed by morphometric, molecular, and functional analyses of KO mice, STAT3 is critically involved in myocardium response to I/R. Infarct sizes after I/R injury were increased in KO hearts, and this was associated with a significant increase in myocardial apoptosis. Previous studies have shown that STAT3 exerts direct cytoprotective effects in cardiomyocytes subjected to ischemia or toxic stress.5,40 The attenuated HSP70 induction in KO myocardium after I/R, a gene known to be transcriptionally regulated by STAT341 and to be involved in protection of the heart from I/R injury,30 suggests that STAT3 exerts at least some of its cardioprotective effects via induction of HSPs. In addition, impaired myocardial capillarization in KO mice may contribute to larger infarcts and enhanced cardiomyocytes apoptosis (eg, by impeding tissue oxygenation during reperfusion). In support of such a concept, induction of BNIP3, which has been identified recently as a hypoxia-regulated inducer of cardiomyocyte death,29 was more pronounced in KO compared with WT mice after I/R injury. In addition, impaired metabolic capacity resulting from reduced perfusion and lower tissue oxygenation during sympathetic stress, as simulated by dobutamine infusion, together with larger infarct size, more interstitial fibrosis, and attenuated hypertrophy, may contribute to the impaired cardiac function and heart failure observed in KO mice after MI. Thus, these data support the concept that STAT3 in cardiomyocytes is providing cardioprotection and cardiac adaptation to I/R and ischemic injury.
In conclusion, STAT3 in cardiomyocytes plays a critical role in myocardium adaptation to stress by maintaining myocardial capillarization, controlling interstitial collagen metabolism, protecting from apoptosis, and preserving cardiac function. STAT3 activation (eg, by IL-6 family members) may therefore represent a beneficial mechanism that should be maintained rather than blocked in patients with heart failure.
| Acknowledgments |
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| Footnotes |
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| References |
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