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Data Supplement for Circulation Research: Volume 91, Issue 6 -- Page 454

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Confocal video sequence of an evoked junctional calcium transient (jCaT) and nerve fiber calcium transients in a pressurized rat mesenteric small artery during subthreshold electrical field stimulation (0.5 Hz). Video images were obtained at the rate of 30 frames per second with the real-time confocal microscope. The images in Figure 1 (A and B) of the accompanying article were taken from this video sequence. In the video, nerve fiber Ca2+ transients are seen as periodic flashes in the nerve fiber bundles. A jCaT, occurring at the time of a nerve fiber Ca2+ transient, is seen in a smooth muscle cell at 4.8 seconds. (Note: Seeing the jCaT in the movie may be aided by referring to Figure 1A of the accompanying article, where a white arrow points to the spot where the jCaT arises.)

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