Published online before print October 24, 2002, doi: 10.1161/01.RES.0000042703.39845.B4
© 2002 American Heart Association, Inc.
Molecular Medicine |
Phorbol Ester Induction of Angiotensin-Converting Enzyme Transcription Is Mediated by Egr-1 and AP-1 in Human Endothelial Cells via ERK1/2 Pathway
From the Institut National de la Santé et de la Recherche Médicale, Unit 525, Faculté de Médecine Pitié-Sapétrière, Paris, France.
Correspondence to Florent Soubrier, INSERM U525, Faculté de Médecine Pitié-Salpétrière-6e Étage, 91, Boulevard de l’Hôpital, 75013 Paris, France. E-mail florent.soubrier{at}chups.jussieu.fr
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Abstract |
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Angiotensin-converting enzyme (ACE) is an enzyme that plays a major role in vasoactive peptide metabolism, and it has been implicated in various cardiovascular diseases. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, has been shown to increase ACE mRNA at the transcriptional level in human umbilical vein endothelial cells. We have investigated the transcriptional mechanism involved in protein kinase C induction of the ACE gene. Deletion and transfection analyses have revealed that two regions are required for PMA-inducible gene expression. The first is a G+C-rich region located in the proximal ACE promoter bearing overlapping consensus recognition sequences for stimulatory protein-1 (Sp1) and early growth response gene 1 (Egr-1). Electrophoretic mobility shift assay and supershift experiments have shown that Egr-1 is present in the specific nucleoprotein complex induced by PMA in human umbilical vein endothelial cells. The second region is located in the distal ACE promoter. DNase I footprinting analysis restricted this region to a 21-bp element containing a cAMP-responsive element/12-O-tetradecanoylphorbol 13-acetate–responsive element sequence. Electrophoretic mobility shift assays and supershift analyses have revealed that activating protein 1 (AP-1) is the transcription factor binding the cAMP-responsive element/12-O-tetradecanoylphorbol 13-acetate–responsive element located in the ACE promoter after PMA stimulation. Mutations of either Egr-1 or AP-1 binding sites partially abrogate ACE expression induced by PMA, whereas mutation of both sites totally abrogates PMA-induced ACE expression. Treatment of cells with PD98059, a mitogen-activated protein kinase kinase-1–specific inhibitor, inhibited PMA-induced ACE expression. Our results demonstrate that the two transcription factors, Egr-1 and AP-1, are involved in the PMA-induced ACE transcriptional activation in human endothelial cells via the activation of the extracellular signal–regulated kinase 1/2 signaling pathway.
Key Words: mitogen-activated protein kinase • angiotensin-converting enzyme • transcriptional regulation • site-directed mutagenesis
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Introduction |
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Angiotensin I–converting enzyme1 (ACE; DCP1, EC 3.4.15.1) is a dipeptidyl carboxypeptidase that plays an important role in the renin-angiotensin system and kallikrein-kinin system. It is a zinc metallopeptidase that generates the vasoconstrictor peptide and growth factor angiotensin II from angiotensin I, inactivates the vasodilator peptide bradykinin, and is able to cleave several other peptides. Two isoenzymes of ACE are found in mammalian tissues, transcribed from a single gene by two alternate promoters.1 Somatic ACE (170 kDa) is synthesized by vascular endothelial cells and several epithelial cells, whereas testis ACE (110 kDa) is expressed exclusively by male germinal cells.2,3
Modulation of ACE expression has been observed in response to various stimuli. ACE expression is induced by steroid hormones in macrophages and bovine pulmonary artery endothelial cells4,5 or by vascular endothelial growth factor (VEGF) in human umbilical vein endothelial cells (HUVECs).6 ACE induction is also observed in several pathophysiological processes in different cell types. ACE expression is increased in early cardiac hypertrophy in rats.7 ACE was also shown to be induced in the aortic wall in different models of rat hypertension, such as the 2-kidney 1-clip hypertensive rat model,8 or in hypertension induced by chronic administration of the NO synthesis inhibitor N
-nitro-L-arginine methyl ester.9
However, only a few studies elucidating the molecular basis of ACE regulation have been performed. In a previous study, we have shown that phorbol 12-myristate 13-acetate (PMA)-activated protein kinase C (PKC) strongly increases ACE activity and ACE mRNA levels. Run-on experiments have demonstrated that PMA induction of ACE mRNA acts at the transcriptional level.10 Because PKC activation represents a common pathway for several factors able to activate endothelial cells and because some of them are known to induce ACE expression, elucidation of the transcriptional mechanism involved in PKC induction of the ACE gene is of particular interest.
In the present study, we characterized the specific nuclear transcription factors that interact with functional cis elements in the ACE promoter in HUVECs stimulated with PMA. Our results demonstrate that an additive effect of two transcription factors, early growth response gene 1 (Egr-1) and activating protein 1 (AP-1), is involved in the PMA-induced ACE transcriptional activation via the Ras–mitogen-activated protein kinase kinase (MEK)–extracellular signal–regulated kinase (ERK) signal transduction pathway.
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Materials and Methods |
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Cell Culture
HUVECs were isolated as described by Jaffe et al.11 Cells were grown in MCDB 131 medium (Sigma Chemical Co) supplemented with 20% heat-inactivated FCS, 2 mmol/L L-glutamine, 100 U/mL penicillin-streptomycin, 10 ng/mL human epidermal growth factor (hEGF), and 1 µg/mL hydrocortisone. Cells were used between passages 2 and 3. Cells were maintained at 37°C and 5% CO2 in a humidified incubator.
DNA Constructs
The ACE promoter–luciferase reporter genes were constructed by cloning a human genomic DNA fragment corresponding to -4335 to 1, which is relative to the transcriptional start site (GenBank accession No. AF229986), into the firefly luciferase reporter gene vector pGL3-basic (Promega). This construct was designated pACE-4335 and was digested by convenient restriction endonucleases for construction of deletion mutants as previously described by Testut et al.12 Restricted fragments were subcloned into pGL3-basic. The plasmids thus obtained were designated pACE-132, pACE-754, pACE-2033, pACE-2795, and pACE-1214. The pACE-1378 construct containing a fragment from -1378 to +1 of the ACE promoter was obtained by partially digesting pACE-2033 by exonuclease III/S1 nuclease using a commercial kit (Erase-a-Base system, Promega).
Expression Vectors
pSCTKr24, the expression vector for Egr-1, was a gift from Dr P. Charnay (Ecole Normale Supérieure, Paris, France). Expression vectors for c-fos and c-jun were kindly provided by Dr M. Yaniv (Institut Pasteur, Paris, France).
Transient Transfection Assays
Cell transfection of plasmid DNA was carried out using polyethyleneimine suspension in a commercially available solution (EXGEN 500, Euromedex). HUVECs (250x103/well) were plated on a 6-well plate, incubated for 24 hours, and then treated with a buffer (150 mmol/L NaCl) containing 0.37 pmol of the relevant reporter gene vector, 0.37 pmol of pRenilla luciferase gene vector (Promega) as the transfection-standardizing control, and 20 µL of EXGEN 500 in a final volume of 100 µL. Cells were incubated overnight with the transfection mix, depleted of serum (0.4%) and of hydrocortisone/hEGF, and treated or not with PMA (100 nmol/L) for 24 hours. Luciferase activity was measured by luminometry using a Dual-Luciferase revelation system (Promega) as described by the manufacturer.
Site-Directed Mutagenesis
Several mutations were introduced in the pACE-1378 construct using an oligonucleotide-directed mutagenesis system (QuickChange site-directed mutagenesis kit, Stratagene) according to the manufacturer’s recommendations. The following oligonucleotides were used to create the desired mutations: Egr1a-mut, 5'-GCGCGGCGGGGCGTTTTCGGGGGTGTGTCG-3'; Egr1b-mut, 5'-GCGCGGCGGGGCGGGGGCGACAGTGTGTCG-3'; and CRE/TRE-mut, 5'-CCCTCTCCCCTGCAACACACTCTCTGACA- AGTCAGTGCG-3'. Mutant clones were screened by DNA sequencing.
Preparation of Nuclear Protein Extracts
HUVECs were grown to subconfluence and then depleted of serum (0.4%) and of hydrocortisone/hEGF for 16 hours before PMA activation. The nuclear extracts used to study Egr-1 binding and AP-1 binding were prepared as previously described by Khachigian et al13 and by Tamaru et al,14 respectively.
Electrophoretic Mobility Shift Assay
Nuclear extracts (5 µg) were incubated with 
0.15 pmol of [
-32P]ATP end-labeled double-stranded oligonucleotides in the binding buffer containing 50 mmol/L NaCl, 5 mmol/L MgCl2, 5% glycerol, 2.5 mmol/L HEPES, 1 µg poly(dI-dC), 20 µg BSA, 0.01% NP 40, 1 mmol/L ZnCl2, 0.5 mmol/L phenylmethylsulfonyl fluoride, and 1 mmol/L dithiothreitol. Complexes were resolved by electrophoresis on prerun acrylamide/bisacrylamide (29:1) native gels. Egr-1, stimulatory protein-1 (Sp1), ATF/CRE binding protein (CREB), and c-fos and c-jun antibodies were from Santa Cruz Biotechnology.
DNase I Footprinting Analysis
A probe corresponding to a fragment of the ACE gene promoter region (-1435 to -1176) was obtained by polymerase chain reaction (PCR) amplification using oligonucleotides Sc10181 (sense, 5'-GACCCCAGCTCTCCTCTCCC-3') and Br12B (antisense, 5'-TGAGTGGGGGATGGTGTCTG-3'). For radioactive labeling, oligonucleotide Br12B (1.5 pmol) was end-labeled with [-32P]ATP by T4 DNA polynucleotide kinase (Life Technologies) before a 20-cycle PCR amplification using 10 pmol of the second primer, Sc10181. DNase I footprinting analysis was performed by incubating 5000 cpm of probe with 50 µg of nuclear extracts for 20 minutes at 20°C, as previously described by Laumonnier et al.15
Computer and Statistical Analysis
ACE gene promoter regions were checked for transcription factor binding sites using Transfac database and MatInspector 2.0 software.16 All transfection data were analyzed by ANOVA.
An expanded Materials and Methods section can be found in the online data supplement available at http://www.circresaha.org.
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Results |
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Identification of Regions in ACE Promoter Required for PMA-Induced Gene Expression in Endothelial Cells
In previous investigations, ACE mRNA levels have been shown to be increased by activation of the PKC signaling system with the phorbol ester PMA.10
To identify cis-acting elements responsible for PMA-induced transcription of the ACE gene, a series of 5'-deletions of a 4335-bp promoter fragment were subcloned into the pGl3-basic vector containing the coding sequence of the firefly luciferase gene. These constructs were transiently transfected in HUVECs, and the promoter activities were measured by luciferase assay. As shown in Figure 1, cells transfected with pACE-4335 induced luciferase activity by 3-fold after treatment with PMA (100 ng/mL). Progressive deletions from -4335 to -1378 of promoter sequence (relative to transcriptional start site) did not significantly affect PMA-induced luciferase activity. Further deletion between -1378 and -1214 resulted in a significant attenuation of the induction by PMA from 3.9 to 2, respectively. A 2-fold induction was maintained with the smallest construct pACE-132 bearing the first 132 bp of the ACE promoter. These results suggest that two PMA-responsive regions exist in the ACE promoter region in HUVECs. The first is a G+C-rich region localized between -132 and 1, and the second region is situated between -1378 and -1214.
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Protein Binding to G+C-Rich ACE Gene Promoter Region in Endothelial Nuclear Extracts
Computer analysis of the G+C-rich ACE gene promoter region (-132/+1) revealed the presence of two overlapping sites for Egr-1/Sp1 transcription factors at -59 bp and -53 bp (Figure 2A). The binding activity of these sites in endothelial nuclear extracts was examined by electrophoretic mobility shift assay (EMSA). EMSA was performed with the use of a 32P-labeled double-stranded oligonucleotide corresponding to the -75 to -46 region of the ACE promoter (Oligo Ra, Figure 2A). A single PMA-induced nucleoprotein complex was observed when 32P-labeled oligonucleotide Ra was incubated with nuclear extracts from HUVECs exposed to 100 ng/mL PMA (Figure 2B, lanes 3 through 7). This complex is specifically competed by the presence of 50- to 100-fold molar excess of the unlabeled probe Ra (Figure 2B, lanes 8 and 9). The addition of a molar excess of mutant competitor (Oligo Ra-m, Figure 2A), in which Egr-1/Sp1 binding sites were disrupted, did not interfere with the formation of the PMA-inducible complex (Figure 2B, lanes 10 and 11). The addition of an unlabeled oligonucleotide containing the Sp1 consensus motif (Figure 2A) did not compete for the formation of this complex (Figure 2B, lanes 12 and 13).
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To identify the nuclear proteins present in the PMA-specific complex, antibody supershift experiments were performed. The PMA-inducible complex was no more detectable when the nuclear extracts were incubated with anti–Egr-1 antibody (Figure 2B, lane 18). The addition of an anti-Sp1 antibody did not affect the formation of any complexes (Figure 2B, lane 15).
All these results indicate that Egr-1 binds the -75 to -46 sequence of the ACE promoter after PMA exposure.
DNase I Footprinting Analysis of ACE Gene Promoter Region (-1378/-1214)
Computer analysis revealed the presence of a large number of putative binding sites for known transcription factors within the (-1378/-1214) region of the ACE promoter. DNase I footprint experiments were performed to determine which sequences in this region are able to bind nuclear proteins. An end-labeled PCR product corresponding to the (-1378/-1254) region of the ACE promoter was used as a probe in footprint experiments with HUVEC nuclear extracts stimulated or not with PMA. As shown in Figure 3A, both nuclear extracts from PMA-treated HUVECs and unstimulated cells protected only one element from DNase I degradation. This element spans 21 bp between -1322 and -1302 and includes a nonconsensus cAMP-responsive element/TPA-responsive element (CRE/TRE) between -1308 to -1315 (TGACGTGT).
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Protein Binding to the ACE Gene Promoter Region (-1322/-1302) in Endothelial Nuclear Extracts
To determine whether the CRE/TRE is able to bind nuclear proteins from PMA-treated HUVECs, we performed a series of competition band-shift and supershift experiments.
The 32P-labeled double-stranded oligonucleotide (Oligo Rb, Figure 3B) bearing the 21-bp element protected in footprinting analysis binding activity of the CRE/TRE was analyzed. A single PMA-induced nucleoprotein complex was observed when 32P-labeled oligonucleotide Rb was incubated with nuclear extracts from HUVECs exposed to 100 ng/mL PMA (Figure 3B, lanes 4 through 7). The addition of a molar excess of the unlabeled probe Rb led to the disappearance of this complex, whereas a molar excess of a mutant competitor with a disrupted CRE/TRE (Oligo Rb-m) did not compete for the formation of this complex (Figure 3B, lanes 8 through 11). Furthermore, the PMA-inducible complex did not appear when a 32P-labeled oligonucleotide Rb-m was incubated with nuclear extracts from HUVECs exposed to PMA (Figure 3B, lanes 12 through 14).
To identify the transcription factors binding the CRE/TRE sequence in nuclear extracts of PMA-treated HUVECs, supershift experiments were performed using a CREB/ATF antibody, reactive with activating transcription factor-1 (ATF-1), CREB-1, and CRE-modulator protein-1 (CREM-1), and a c-fos and a c-jun antibody. Only the addition of a c-jun antibody completely blocked the formation of the PMA-inducible complex (Figure 3B, lanes 15 through 20). These results suggest that an AP-1 complex containing a c-jun homodimer is able to bind the CRE/TRE sequence of the ACE promoter in response to PMA.
Functional Analysis of Egr-1 and AP-1 Binding Sites of the ACE Gene Promoter
To test the functionality of Egr-1 and AP-1 binding sites, eucaryotic expression vectors encoding Egr-1, c-jun, and c-fos or an empty control vector (pcDNA3.1) were cotransfected with pACE-1378 in HUVECs, and the promoter activity was assessed. Coexpression of the expression vector encoding for Egr-1 (Figure 4A) or c-jun alone (Figure 4B) dose-dependently activated the pACE-1378 transcriptional activity, whereas coexpression of c-jun and c-fos (Figure 4B) had no effect on the transcriptional activation of pACE-1378. These results suggest that both Egr-1 and c-jun functionally interact with the ACE promoter to induce ACE expression but that c-fos is not involved.
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We prepared a set of site-directed mutants with sequence variations in either or both the Egr-1 and the AP-1 binding sites. The mutations were introduced in the context of the p1378 construct that had a full functional activity in response to PMA by use of a PCR-based mutagenesis with mutant oligonucleotides. These constructs were transfected in HUVECs, and PMA-induced promoter activity was determined (Figure 5). PMA-stimulated luciferase activity of the pACE-1378m1 construct, in which the Egr-1 binding site in position -59 was disrupted, was 1.85-fold. Interestingly, disruption of the second Egr-1 binding site in position -53 (pACE-1378 m2) had no effect on ACE promoter activity in response to PMA. Indeed, transfection with pACE-1378 m2 resulted in a 2.63-fold activation of luciferase activity in response to PMA, which was not significantly different from the 3-fold increase observed with the wild-type construct. HUVECs transfected with the pACE-1378 m3 construct, with a mutated CRE/TRE sequence, expressed a luciferase activity that was 1.8 higher when exposed to PMA. To determine whether only the Egr1(-59) binding site and the CRE/TRE participate in the PMA-inducible expression of the ACE gene, we made a reporter construct in which both sites were disrupted, pACE-1378 m4. These mutations totally abolished the PMA-promoted induction.
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These findings demonstrate the requirement of both intact Egr-1 (in position -59) and CRE/TRE (in position -1308 to -1315) binding sites in the ACE promoter sequence for PMA-inducible expression.
ERK1/2 Pathway Is Involved in PMA-Induced ACE Expression
The contribution of ERK1/2 activation to the PMA-mediated transcriptional activity of the ACE gene promoter was assessed in cells transfected with the pACE-1378 construct and pretreated for 30 minutes with PD98059, a MEK-1–specific inhibitor (5 µmol/L in 0.1% dimethyl sulfoxide [DMSO]), or with 0.1% DMSO alone before being subjected or not to PMA treatment (100 ng/mL) for 24 hours. The data in Figure 6A show that under these experimental conditions, PMA increased luciferase activity by 3-fold. PD98059 totally inhibited this effect, suggesting that PMA signal transduction pathway in HUVECs requires activated MEK-1. The inhibitory effect of PD98059 on PMA-induced luciferase activity was observed when cells were transfected by either pACE-1378m1, whose Egr-1 (-59) binding site is disrupted, and pACE-1378m3, whose AP-1 binding site is disrupted. These results suggest that the ERK1/2 pathway is involved in the activation of both transcription factors (Egr-1 and AP-1), thus leading to ACE transcriptional induction in response to PMA in HUVECs.
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We determined the effects of PD98059 on PMA-induced interaction between Egr-1 and AP-1 proteins with the ACE promoter. Nuclear extracts from HUVECs treated with 0.1% DMSO, PD98059 (5 µmol/L in 0.1% DMSO), PMA (100 ng/mL in 0.1% DMSO), or both PD98059 and PMA were incubated with the 32P-labeled oligonucleotide Ra (Figure 6B, lanes 1 through 8) or with the 32P-labeled oligonucleotide Rb (Figure 6B, lanes 9 through 14). In both cases, PMA treatment induced the formation of a specific nucleoprotein complex that was no more visible in the presence of nuclear extracts treated by both PMA and PD98059. Supershift experiments confirmed that the nucleoprotein complex formed in the presence of the 32P-labeled oligonucleotide Ra contains Egr-1, whereas the nucleoprotein complex formed in the presence of the 32P-labeled oligonucleotide Rb contains c-jun. These results indicate that ERK1/2 is directly involved in the binding of the Egr-1 and AP-1 transcription factors to the ACE promoter after PMA stimulation.
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Discussion |
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Our previous study showed that PMA treatment was responsible for increasing ACE transcription in HUVECs. In the present study, we investigated the molecular mechanisms of the PMA-regulated ACE expression in endothelial cells.
A 4335-bp fragment of the ACE promoter was analyzed for the presence of PMA-responsive elements. 5'-Deletion analysis of the ACE promoter indicates that two distinct regions are required for PMA-induced ACE expression. The first is a G+C-rich region located in the proximal promoter (-132/+1). This region includes two putative binding sites for the Egr-1 transcription factor in positions -53 and -59, overlapping with two Sp1 potential binding sites. EMSA using PMA-treated endothelial cell nuclear extracts demonstrated that the formation of a nucleoprotein complex was induced. Supershift analysis revealed that Egr-1 is the nuclear factor that binds specifically to this region of the ACE promoter. The zinc-finger transcription factor Egr-1 is an "immediate-early response protein" that is rapidly and transiently induced by a large number of growth factors, cytokines, and injurious stimuli.17 A model for the cascade of molecular events underlying the inducible expression of Egr-1–dependent genes in vascular endothelial cells has been proposed: multiple extracellular stimuli activate phosphorylation-dependent signaling pathways, which stimulate Egr-1 expression and activation. In the nucleus, Egr-1 binds to an element overlapping the Sp1 site, displaces the prebound Sp1, occupies the GC box, and stimulates transcription of the target gene.18 This mechanism has been proposed for PMA-stimulated expression of platelet-derived growth factor-B or tissue factor gene, but other genes, such as the basic transcription regulatory element binding protein 2, have been shown to be regulated by Egr-1 in response to PMA stimulation in vascular smooth muscle cells, without Sp1 displacing.19 In the case of the ACE promoter, Egr-1 seems not to displace prebound Sp1 because appearance of the Egr-1 complex is not accompanied by displacement of another complex and because none of the observed complexes are affected by the presence of a molar excess of a consensus sequence for the Sp1 binding site or an Sp1 antibody.
EMSA experiments showed that PMA-treated nuclear extracts bind the two overlapping Egr-1 binding sites found at positions -53 and -59. In contrast, only the -59 binding site is functional because the mutation of this site decreased by 40% the induction of ACE in response to PMA, whereas mutation of the putative binding site in position -53 did not affect this response.
Footprinting analysis of the region (-1378 to -1214) implicated in PMA regulation revealed a protected element between -1322 and -1302, including a nonconsensus CRE/TRE sequence. Consensus sequences of CRE (TGACGTCA) and TRE (TGAGTCA) are closely related, inasmuch as they differ by a single nucleotide. The sequence of the CRE/TRE-like element within the ACE gene promoter (TGACGTGT) differs by two nucleotides from the CRE consensus and by three from the TRE consensus sequence. The consensus CRE sequence is known to bind CREB, whereas the consensus TRE sequence preferentially binds AP-1. However, the similarity between the two regulatory elements may allow cross talk between components of these two major signal transduction pathways.20 EMSA revealed that PMA induces the formation of a nucleoprotein complex on the CRE/TRE sequence. This EMSA complex is not visible with nuclear extracts not treated with PMA, whereas the protected element detected by footprinting experiments was visible with nuclear extracts that were treated or not with PMA. One explanation could be that other proteins could bind this region of the ACE promoter independently of the presence of PMA; this is consistent with the fact that two nucleoprotein complexes are visible in EMSA with nuclear extracts not submitted to PMA treatment. The nature of the proteins involved in these complexes was not determined. To identify the nuclear factor involved in the formation of the PMA-induced complex, supershift analysis was performed. A CREB/ATF antibody that reacts with ATF-1, CREB-1, and CREM-1 had no effect on the mobility of the PMA-induced complex. A c-jun antibody is able to block the formation of this complex, whereas a c-fos antibody has no effect. This result indicates that an AP-1 complex transcription factor formed by a jun homodimer binds the ACE promoter on the CRE/TRE in position -1308 on PMA exposure. Treatment by phorbol ester is one of the extracellular stimuli known to activate the mitogen-activated protein kinase signaling pathway.21 Furthermore, AP-1 and Egr-1 are known to be activated by the ERK1/2 or the c-Jun NH2-terminal kinase (JNK) pathway in different cell types.22,23 We found that transfection of a dominant-negative form of JNK has no effect on the transcriptional activation of ACE in response to PMA (please see online expanded Materials and Methods section), whereas the MEK-1–specific inhibitor PD98059 totally abolishes the response of the ACE promoter to PMA in a reporter construct, suggesting that signal transduction via ERK1/2 is predominantly involved in the PMA activation of ACE transcription through both the AP-1 and Egr-1 response elements present in the 1378-bp promoter fragment. In HUVECs, PD98059 at the same concentration (5 µmol/L) does not completely inhibit the induction of ACE mRNA expression (please see online data supplement). The cascade of events leading to transcriptional ACE upregulation in response to phorbol ester stimulation is summarized in Figure 7.
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The PKC signaling is a convergent path for various extracellular stimuli; thus, the new transcription induction pathway of the ACE gene described in the present study might represent a mechanism of ACE gene activation in various pathophysiological processes that involve PKC activation. A direct link between growth factor stimulation, PKC activation, and ACE upregulation has recently been established in endothelial cells. VEGF was shown to induce ACE mRNA levels in HUVECs. This effect was inhibited in the presence of a selective inhibitor of PKC, suggesting that VEGF induction of ACE is PKC dependent.6 However, we did not find any significant difference in reporter transcription activity between cells submitted to VEGF treatment or not (data not shown) by transfection with the pACE-1378 construct, which had a full functional activity in response to PMA, in HUVECs cultured in medium in the presence or absence of VEGF (50 ng/mL). Thus, ACE upregulation induced by VEGF and mediated by PKC seems not to act through a transcriptional mechanism involving the first 1378 bp of the ACE promoter in HUVECs. Shear stress is another pathophysiological stimulus candidate for being involved in ACE upregulation through the molecular pathway described in the present study. Various arguments are in favor of this hypothesis. First, ACE expression is upregulated by shear stress in the vascular cells of rats.24 Second, PKC is one of the intracellular mediators of the shear stress signal transduction pathway. Furthermore, the molecular cascade of signalization that we described shares many similarities with transduction pathways known to mediate the transcriptional regulation of various genes in response to shear stress. Thus, the ERK1/2 pathway is activated by shear stress in vascular endothelial cells, leading to the activation of Egr-1 and AP-1 transcription factors.25,26 In summary, we demonstrated the functional role of two major elements for transcriptional regulation of ACE. These elements could participate in the modulation of ACE expression observed in major physiological and pathological conditions.
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Acknowledgments |
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This study was supported by INSERM, by a grant of the French Ministry of Research, and by an unrestricted grant from Bristol-Myers-Squibb. Dr Eyries was supported by a PhD grant from the Société Francaise d’Athérosclérose, the Fondation de France, and the Fondation pour la Recherche Médicale. We thank Dr P. Charnay for the gift of the Egr-1 expression construct and Dr M. Yaniv for the gift of the c-fos and c-jun expression vectors. We are grateful to Dr B. Derijard for kindly providing the wild-type JNK and the dominant-negative JNK expression vectors.
Received March 14, 2002; revision received September 19, 2002; accepted October 10, 2002.
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References |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
|---|
- Hubert C, Houot AM, Corvol P, Soubrier F. Structure of the angiotensin I-converting enzyme gene: two alternate promoters correspond to evolutionary steps of a duplicated gene. J Biol Chem. 1991; 266: 15377–15383.
[Abstract/Free Full Text] - Erdos EG, Skidgel RA. The angiotensin I-converting enzyme. Lab Invest. 1987; 56: 345–348.[Medline] [Order article via Infotrieve]
- Soubrier F, Alhenc-Gelas F, Hubert C, Allegrini J, John M, Tregear G, Corvol P. Two putative active centers in human angiotensin I-converting enzyme revealed by molecular cloning. Proc Natl Acad Sci U S A. 1988; 85: 9386–9390.
[Abstract/Free Full Text] - Friedland J, Setton C, Silverstein E. Angiotensin converting enzyme: induction by steroids in rabbit alveolar macrophages in culture. Science. 1977; 197: 64–65.
[Abstract/Free Full Text] - Dasarathy Y, Lanzillo JJ, Fanburg BL. Stimulation of bovine pulmonary artery endothelial cell ACE by dexamethasone: involvement of steroid receptors. Am J Physiol. 1992; 263: L645–L649.[Medline] [Order article via Infotrieve]
- Saijonmaa O, Nyman T, Kosonen R, Fyhrquist F. Upregulation of angiotensin-converting enzyme by vascular endothelial growth factor. Am J Physiol. 2001; 280: H885–H891.
- Schunkert H, Dzau VJ, Tang SS, Hirsch AT, Apstein CS, Lorell BH. Increased rat cardiac angiotensin converting enzyme activity and mRNA expression in pressure overload left ventricular hypertrophy: effects on coronary resistance, contractility, and relaxation. J Clin Invest. 1990; 86: 1913–1920.[Medline] [Order article via Infotrieve]
- Arnal JF, Battle T, Rasetti C, Challah M, Costerousse O, Vicaut E, Michel JB, Alhenc-Gelas F. ACE in three tunicae of rat aorta: expression in smooth muscle and effect of renovascular hypertension. Am J Physiol. 1994; 267: H1777–H1784.[Medline] [Order article via Infotrieve]
- Takemoto M, Egashira K, Usui M, Numaguchi K, Tomita H, Tsutsui H, Shimokawa H, Sueishi K, Takeshita A. Important role of tissue angiotensin-converting enzyme activity in the pathogenesis of coronary vascular and myocardial structural changes induced by long-term blockade of nitric oxide synthesis in rats. J Clin Invest. 1997; 99: 278–287.[Medline] [Order article via Infotrieve]
- Villard E, Alonso A, Agrapart M, Challah M, Soubrier F. Induction of angiotensin I-converting enzyme transcription by a protein kinase C-dependent mechanism in human endothelial cells. J Biol Chem. 1998; 273: 25191–25197.
[Abstract/Free Full Text] - Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins: identification by morphologic and immunologic criteria. J Clin Invest. 1973; 52: 2745–2756.[Medline] [Order article via Infotrieve]
- Testut P, Soubrier F, Corvol P, Hubert C. Functional analysis of the human somatic angiotensin I-converting enzyme gene promoter. Biochem J. 1993; 293: 843–848.[Medline] [Order article via Infotrieve]
- Khachigian LM, Williams AJ, Collins T. Interplay of Sp1 and Egr-1 in the proximal platelet-derived growth factor A-chain promoter in cultured vascular endothelial cells. J Biol Chem. 1995; 270: 27679–27686.
[Abstract/Free Full Text] - Tamaru M, Narumi S. E-selectin gene expression is induced synergistically with the coexistence of activated classic protein kinase C and signals elicited by interleukin-1ß but not tumor necrosis factor-
. J Biol Chem. 1999; 274: 3753–3763.[Abstract/Free Full Text] - Laumonnier Y, Nadaud S, Agrapart M, Soubrier F. Characterization of an upstream enhancer region in the promoter of the human endothelial nitric-oxide synthase gene. J Biol Chem. 2000; 275: 40732–40741.
[Abstract/Free Full Text] - Quandt K, Frech K, Karas H, Wingender E, Werner T. MatInd and MatInspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucleic Acids Res. 1995; 23: 4878–4884.
[Abstract/Free Full Text] - Gashler A, Sukhatme VP. Early growth response protein 1 (Egr-1): prototype of a zinc-finger family of transcription factors. Prog Nucleic Acid Res Mol Biol. 1995; 50: 191–224.[Medline] [Order article via Infotrieve]
- Khachigian LM, Lindner V, Williams AJ, Collins T. Egr-1-induced endothelial gene expression: a common theme in vascular injury. Science. 1996; 271: 1427–1431.[Abstract]
- Kawai-Kowase K, Kurabayashi M, Hoshino Y, Ohyama Y, Nagai R. Transcriptional activation of the zinc finger transcription factor BTEB2 gene by Egr-1 through mitogen-activated protein kinase pathways in vascular smooth muscle cells. Circ Res. 1999; 85: 787–795.
[Abstract/Free Full Text] - Busch SJ, Sassone-Corsi P. Fos, Jun, and CREB basic-domain peptides have intrinsic DNA-binding activity enhanced by a novel stabilizing factor. Oncogene. 1990; 5: 1549–1556.[Medline] [Order article via Infotrieve]
- Kazlauskas A, Cooper JA. Protein kinase C mediates platelet-derived growth factor-induced tyrosine phosphorylation of p42. J Cell Biol. 1988; 106: 1395–1402.
[Abstract/Free Full Text] - Dieckgraefe BK, Weems DM. Epithelial injury induces egr-1 and fos expression by a pathway involving protein kinase C and ERK. Am J Physiol. 1999; 276: G322–G330.[Medline] [Order article via Infotrieve]
- Karin M. The regulation of AP-1 activity by mitogen-activated protein kinases. J Biol Chem. 1995; 270: 16483–16486.
[Free Full Text] - Gosgnach W, Challah M, Coulet F, Michel JB, Battle T. Shear stress induces angiotensin converting enzyme expression in cultured smooth muscle cells: possible involvement of bFGF. Cardiovasc Res. 2000; 45: 486–492.
[Abstract/Free Full Text] - Schwachtgen JL, Houston P, Campbell C, Sukhatme V, Braddock M. Fluid shear stress activation of egr-1 transcription in cultured human endothelial and epithelial cells is mediated via the extracellular signal-related kinase 1/2 mitogen-activated protein kinase pathway. J Clin Invest. 1998; 101: 2540–2549.[Medline] [Order article via Infotrieve]
- Jalali S, Li YS, Sotoudeh M, Yuan S, Li S, Chien S, Shyy JY. Shear stress activates p60src-Ras-MAPK signaling pathways in vascular endothelial cells. Arterioscler Thromb Vasc Biol. 1998; 18: 227–234.
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