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Data Supplement for Circulation Research: Volume 86, Issue 7 -- Page 745

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Data Files

Movie1.mpg

Movie 1. Time-lapse movie of deconvolved optical section demonstrating intracellular GFP-vimentin IF movement within the first few minutes of flow onset. IF motion in 90-sec intervals (green) before and after the start of flow was superimposed on stationary images (red) representing the IF distribution at the start of the no-flow and flow periods, respectively. Separation of green and red illustrates IF movement; yellow represents zero displacement compared with the beginning of the period. Flow direction, left to right; shear stress, 12 dyn/cm². Scale bars, 2 µm. Elapsed time, mm:ss.

Movie2.mpg

Movie 2. Time-lapse movie of deconvolved optical section showing GFP-vimentin IF near the apical cell surface above the nucleus, corresponding to the region shown in Figure 2B. IF motion in 90-sec intervals (green) before and after the start of flow was superimposed on stationary images (red) representing the IF distribution at the start of the no-flow and flow periods, respectively. Separation of green and red illustrates IF movement; yellow represents zero displacement compared with the beginning of the period. Flow direction, left to right; shear stress, 12 dyn/cm². Scale bars, 2 µm. Elapsed time, mm:ss.

Prepared by: the Data Supplement Manager

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