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Circulation Research. 2000;86:255-263

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(Circulation Research. 2000;86:255.)
© 2000 American Heart Association, Inc.


Integrative Physiology

Calcineurin-Mediated Hypertrophy Protects Cardiomyocytes From Apoptosis In Vitro and In Vivo

An Apoptosis-Independent Model of Dilated Heart Failure

Leon J. De Windt, Hae W. Lim, Tyler Taigen, Detlef Wencker, Gianluigi Condorelli, Gerald W. Dorn, II, Richard N. Kitsis, Jeffery D. Molkentin

From the Departments of Pediatrics (L.J.D.W., H.W.L., T.T., J.D.M.) and Cardiology (G.W.D.), University of Cincinnati, and Division of Molecular Cardiovascular Biology (L.J.D.W., H.W.L., T.T., J.D.M.), Children’s Hospital Medical Center, Cincinnati, Ohio; Departments of Medicine and Cell Biology (D.W., R.N.K.), Albert Einstein College of Medicine, The Bronx, NY; and Kimmel Cancer Center and Department of Microbiology and Immunology (G.C.), Thomas Jefferson University, Philadelphia, Pa.

Correspondence to Jeffery D. Molkentin, Department of Pediatrics, Division of Molecular Cardiovascular Biology, Children’s Hospital Medical Center, 3333 Burnet Ave, Cincinnati, OH 45229-3039. E-mail molkj0{at}chmcc.org


*    Abstract
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*Abstract
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Abstract—We have previously shown that the calcium-calmodulin–regulated phosphatase calcineurin (PP2B) is sufficient to induce cardiac hypertrophy that transitions to heart failure in transgenic mice. Given the rapid onset of heart failure in these mice, we hypothesized that calcineurin signaling would stimulate myocardial cell apoptosis. However, utilizing multiple approaches, we determined that calcineurin-mediated hypertrophy protected cardiac myocytes from apoptosis, suggesting a model of heart failure that is independent of apoptosis. Adenovirally mediated gene transfer of a constitutively active calcineurin cDNA (AdCnA) was performed in cultured neonatal rat cardiomyocytes to elucidate the mechanism whereby calcineurin affected myocardial cell viability. AdCnA infection, which induced myocyte hypertrophy and atrial natriuretic factor expression, protected against apoptosis induced by 2-deoxyglucose or staurosporine, as assessed by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) labeling, caspase-3 activation, DNA laddering, and cellular morphology. The level of protection conferred by AdCnA was similar to that of adenoviral Bcl-xL gene transfer or hypertrophy induced by phenylephrine. In vivo, failing hearts from calcineurin-transgenic mice did not demonstrate increased TUNEL labeling and, in fact, demonstrated a resistance to ischemia/reperfusion–induced apoptosis. We determined that the mechanism whereby calcineurin afforded protection from apoptosis was partially mediated by nuclear factor of activated T cells (NFAT3) signaling and partially by Akt/protein kinase B (PKB) signaling. Although calcineurin activation protected myocytes from apoptosis, inhibition of calcineurin with cyclosporine was not sufficient to induce TUNEL labeling in Gq{alpha}-transgenic mice or in cultured cardiomyocytes. Collectively, these data identify a calcineurin-dependent mouse model of dilated heart failure that is independent of apoptosis.


Key Words: calcineurin • apoptosis • cardiac hypertrophy • phenylephrine • caspase-3


*    Introduction
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*Introduction
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Heart failure can result from a wide range of pathological conditions such as infarction, chronic left ventricular hypertrophy, ischemic cardiomyopathy, idiopathic dilated cardiomyopathy, hypertrophic cardiomyopathy, valvular heart disease, and viral infection. In response to these pathologic conditions, cardiomyocytes may undergo apoptosis, or programmed cell death, which has been postulated to contribute to the progressive pathology associated with heart failure.1 2 3 4 Apoptosis is an energy-requiring process that results in cellular shrinkage, nuclear condensation, and DNA fragmentation in response to developmental cues or to activation/inhibition of specific reactive signaling cascades (reviewed in References 5 and 65 6 ).

Increased hypertrophic signaling via either of the heterotrimeric G proteins, Gq{alpha} or Gs{alpha}, in transgenic mouse hearts was shown to promote cardiomyocyte apoptosis and cardiomyopathy, suggesting that overstimulation of G protein–coupled receptors might promote cell death.7 8 Consistent with this interpretation, pharmacological stimulation of ß-adrenergic receptors in vivo caused apoptosis independent of left ventricular hypertrophy or tachycardia.9 In vitro studies in cultured primary cardiomyocytes also implicate certain signaling pathways in apoptosis. Stretch-mediated release of angiotensin II is associated with p53 activation and cardiomyocyte apoptosis.10 11 Similarly, atrial natriuretic factor (ANF) and tumor necrosis factor-{alpha} likely promote cardiomyocyte apoptosis.12 13 Mitogen-activated protein kinase (MAPK) activation is also thought to be a regulator of apoptosis. Specifically, activation of p38{alpha} may promote cardiomyocyte apoptosis.14 15

In contrast, numerous studies have demonstrated protection from apoptosis through activation of certain intracellular signaling pathways. Cardiotrophin-1 signaling through the gp130 receptor was shown to protect cardiomyocytes from apoptosis.16 Induction of cardiac hypertrophy through MKK6 and P38ß has also been associated with protection from apoptosis.14 15 Insulin-like growth factor-1 (IGF-1) stimulation protects cardiomyocytes from apoptosis through phosphatidylinositol 3-kinase and MAPK-dependent signaling pathways.17 18 19 Hypertrophic agonists such as phenylephrine (PE) and endothelin-1 are also associated with protection from apoptosis, suggesting that hypertrophy itself may confer protection.20

We recently described a novel mediator of cardiac hypertrophy through the calcium-calmodulin–regulated intracellular phosphatase calcineurin (PP2B) and the transcriptional regulator nuclear factor of activated T cells (NFAT3).21 Expression of a constitutively active form of calcineurin in transgenic mouse hearts resulted in profound cardiac hypertrophy that progressed to dilated heart failure within 8 to 12 weeks.21 It was of interest to determine whether hypertrophic signaling mediated by calcineurin induced apoptosis, which might explain the rapid progression to heart failure in these mice. However, we determined that cardiomyocyte terminal deoxynucleotidyltransferase–mediated dUTP nick end labeling (TUNEL) in calcineurin-transgenic hearts is not significantly different from that in wild-type hearts. In fact, calcineurin-transgenic hearts were significantly less susceptible to ischemia/reperfusion–induced apoptosis than were wild-type hearts. To define the mechanism whereby calcineurin signaling might protect cardiac myocytes from apoptosis, we generated a calcineurin adenovirus (AdCnA) to infect cultured rat neonatal cardiomyocytes. AdCnA infection induced a hypertrophic response that was protective against staurosporine- and 2-deoxyglucose–induced apoptosis. The likely mechanism whereby calcineurin protects myocytes from apoptosis is associated with NFAT3 and Akt/protein kinase B (PKB) activation.


*    Materials and Methods
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*Materials and Methods
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The mouse model of ischemia/reperfusion by ligation of the left descending coronary artery was performed as described previously.22 Primary cultures of neonatal rat cardiomyocytes were obtained as described previously.23 E1a-deleted, replication-deficient adenovirus expressing a constitutively activated form of mouse calcineurin A{alpha} amino acids 1 to 398 (AdCnA), full-length human Bcl-xL (AdBcl-xL), or a constitutively nuclear form of NFAT3 (AdNFAT3) was initially constructed in pACCMV-pLpA and cotransfected into HEK293 cells with pJM17 as described before.24 For the generation of the adenovirus expressing a calcineurin-inhibitory peptide (Adcain), a 582-bp fragment corresponding to amino acids 1989 to 2182 of cain25 26 was generated by polymerase chain reaction and subcloned with a flag epitope as a HindIII fragment into pACCMVpLpA. All initial recombinants were plaque-purified, expanded, and titered by duplicate plaque assays in monolayers of HEK293 using the agarose gel overlay method.27 Adenoviral infection of cardiomyocytes was performed at a multiplicity of infection of 100 plaque-forming units in 2 mL (6-cm culture dishes) of DMEM supplemented with 2% FBS for 2 hours at 37°C in a humidified, 5% CO2 incubator. Under these infection conditions, {approx}99% of the cells were positive for protein expression by immunocytochemistry or stained ß-galactosidase positive after 24 hours. TUNEL labeling of cultured cardiomyocytes or tissue sections was performed with the CardioTACS kit (Trevigen) as recommended by the manufacturer. Cardiomyocytes were prepared for immunocytochemistry as described previously.21 Data are expressed as mean±SEM, and differences were evaluated for significance using the Student t test for unpaired data or 1-way ANOVA followed by the Bonferroni post test when appropriate.

An expanded Materials and Methods section is available online at http://www.circresaha.org.


*    Results
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up arrowMaterials and Methods
*Results
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Calcineurin Adenovirus Drives Neonatal Cardiomyocyte Hypertrophy In Vitro
To investigate the importance of calcineurin signaling in vitro, we generated a replication-deficient adenovirus expressing the constitutively active form of mouse calcineurin A{alpha} (AdCnA) (amino acids 1 to 398) to perform gene transfer in cultured cardiac myocytes. Consistent with the effect of activated calcineurin expression in vivo, AdCnA induced morphological hypertrophy, whereas control infection with a ß-galactosidase adenovirus (Adßgal) had no effect (Figure 1ADown through 1H). The hypertrophic response was independent of cellular density and produced a quantitative increase in cell surface area 96 hours after infection (Figure 1IDown). AdCnA-infected cardiomyocytes also demonstrated greater sarcomeric organization (Figure 1EDown and 1FDown), rhythmic beating 24 hours after infection, and ANF protein expression (Figure 1KDown). Quantification of ANF expression in a representative experiment revealed that among cardiomyocytes, 9.2% (±2.4%) of those infected with Adßgal, 39% (±2.5%) of those infected with AdCnA, and 54% (±2.7%) of those treated with PE were positive (P<0.05). These data indicate that expression of activated calcineurin is sufficient to drive the hypertrophic program in cultured neonatal cardiomyocytes.



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Figure 1. AdCnA infection of either low- or high-density neonatal cardiomyocytes induces hypertrophy (E and F), similarly to PE treatment (G and H), whereas infection with Adßgal had no hypertrophic effect (C and D) compared with uninfected (A and B). Cells were stained with {alpha}-actinin antibody (red), and nuclei were stained with bis-benzamide (white). I, Quantification of AdCnA-mediated hypertrophy demonstrated a significant increase in cell surface area compared with Adßgal infection. Data are from at least 3 independent experiments performed in duplicate (*P<0.05 vs Adßgal). J through L, Cardiomyocytes were also stained for ANF protein expression 48 hours after infection, demonstrating that AdCnA and PE induce ANF expression, whereas Adßgal had no effect.

Calcineurin Adenoviral Infection Protects Cardiomyocytes From Apoptosis
Activation of certain intracellular signaling pathways in cardiomyocytes has been shown to profoundly affect myocardial cell viability. It was of interest to determine whether constitutive calcineurin activation would promote or protect cardiomyocytes from cell death. To this end, we determined that AdCnA infection of cultured neonatal cardiomyocytes did not induce apoptosis over any time period compared with Adßgal infection or uninfected cells (data not shown). In contrast, AdCnA infection was found to protect cultured cardiomyocytes from apoptosis induced by 2 different pharmacological agents. Cells were cultured for 24 hours after adenoviral infection and treated with either staurosporine (1.0 µmol/L) or 2-deoxyglucose (2 mmol/L) in glucose- and serum-free medium. Staurosporine was previously shown to induce cardiomyocyte apoptosis in a caspase-dependent manner.28 Similarly, metabolic inhibition with 2-deoxyglucose was also shown to induce apoptosis in cultured cardiomyocytes.29 30 The data show that AdCnA infection protected cardiomyocytes from morphological features associated with cell death, whereas Adßgal infection had no protective effect (Figure 2CDown, 2DDown, 2GDown, and 2HDown). As a positive control for these assays, we generated an adenovirus expressing the antiapoptotic gene Bcl-xL. AdBcl-xL infection also protected cardiomyocytes from both staurosporine- and 2-deoxyglucose–mediated effects (Figure 2EDown and 2FDown). As a further control, the calcineurin-inhibitory drug cyclosporine was added to AdCnA-infected cardiomyocytes, resulting in a reversal of protection. However, cyclosporine alone did not induce apoptosis in untreated cardiomyocytes in serum-free medium (data not shown).



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Figure 2. AdCnA or AdBcl-xL infection protects neonatal cardiomyocytes from morphological changes associated with 2-deoxyglucose– and staurosporine-mediated cell death (E through H), whereas Adßgal infection was not protective (C and D) similar to uninfected (A and B). Neonatal cardiomyocytes were infected with adenovirus for 2 hours, cultured for 24 additional hours, and then treated with either 2 mmol/L 2-deoxyglucose in glucose and serum-free medium (24 hours) or with 0.5 µmol/L staurosporine in serum-free medium (18 hours). Cyclosporine cotreatment (500 ng/mL) reversed the protective effect of AdCnA infection (I and J). Cells were stained with {alpha}-actinin antibody (red), and nuclei were stained with bis-benzamide (white).

TUNEL assays were performed to quantify the induction of cell death by staurosporine and 2-deoxyglucose. Only 2% to 4% of nontreated cardiomyocytes were TUNEL-positive, whereas staurosporine or 2-deoxyglucose treatment induced 17% and 26% TUNEL-positive staining, respectively (P<0.05) (Figure 3Down). Consistent with cellular morphology shown above, AdCnA and AdBcl-xL infection significantly reduced staurosporine- and 2-deoxyglucose–induced cell death, whereas control Adßgal infection had no protective effect (P<0.05) (Figure 3Down). Adenoviral delivery routinely resulted in 98% to 99% cardiomyocyte infection, so that uninfected cells did not appreciably influence quantification. The protective effect of AdCnA was only partially reversed by cyclosporine (P<0.05), likely because this agent is not a complete inhibitor of calcineurin. This interpretation is consistent with the known profile of only partial immune suppression in vivo, even at a high dosage of cyclosporine.31 AdCnA infection also significantly protected cardiomyocytes from staurosporine- or 2-deoxyglucose–induced caspase-3 activation, poly(ADP-ribose) polymerase and protein kinase C{delta} degradation, and DNA laddering compared with Adßgal infection (data not shown). We and others have previously characterized that 2-deoxyglucose or staurosporine treatment of cultured cardiomyocytes results in caspase activation and cytochrome c release.28 29 30



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Figure 3. AdCnA infection protects cardiomyocytes from TUNEL labeling induced by 2-deoxyglucose (A) and staurosporine (B). Under both cell death–promoting treatments, AdCnA infection, AdBcl-xL infection, and PE stimulation significantly reversed the number of TUNEL-positive cardiomyocytes, whereas cotreatment with cyclosporine (CsA) partially blocked AdCnA-mediated protection. Data represent 4 independent experiments performed in duplicate. {dagger}P<0.05 vs Adßgal, *P<0.05 vs control, #P<0.05 vs AdCnA.

Activation of Endogenous Calcineurin Protects Myocytes From Apoptosis
It was previously reported that {alpha}-adrenergic stimulation (PE) protected cultured cardiomyocytes from apoptosis.20 We have determined that PE stimulation leads to calcineurin activation in cultured cardiomyocytes.31a To investigate the potential involvement of calcineurin in PE-mediated protection from apoptosis, we treated cardiomyocytes with cyclosporine or a calcineurin-inhibitory adenovirus. As previously reported, we also found that PE significantly protected cardiomyocytes from staurosporine- and 2-deoxyglucose–induced cell death (Figure 3Up). Remarkably, inhibition of calcineurin with cyclosporine partially reversed the antiapoptotic effects of PE to 2-deoxyglucose (Figure 4Down). Because cyclosporine is known to affect proteins other than calcineurin, we also specifically targeted calcineurin by adenoviral expression of a 194–amino acid inhibitor peptide from the cain protein.25 26 Adcain infection significantly reversed the antiapoptotic effects of PE hypertrophy to 2-deoxyglucose (Figure 4Down), whereas Adßgal had no effect. Adcain infection alone in serum-free medium did not induce cell death (data not shown). These data indicate that calcineurin is a necessary component of PE-mediated protection from cardiomyocyte cell death in vitro. These data also indicate that physiological activation of endogenous calcineurin affords protection from cell death.



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Figure 4. Significant reversal of PE-mediated protection from 2-deoxyglucose–induced TUNEL labeling by cyclosporine (CsA), Adcain, and PD98059 (MEK1 inhibitor), whereas SB202190 (p38 inhibitor) did not relieve protection. These data suggest that calcineurin and ERK activation protect cardiomyocytes from cell death. *P<0.05 vs no adenovirus (No Ad); {dagger}P<0.05 vs PE. Data are from 4 independent experiments performed in duplicate.

It was also of interest to determine whether MAPK signaling factors played a role in PE-mediated protection from apoptosis. Inhibition of p38-reactive signaling with SB202190 (20 µmol/L) did not reverse the protective effect of PE, whereas inhibition of extracellular signal–regulated kinase (ERK) signaling with the mitogen-activated protein/ERK (MEK)–1 inhibitor PD98059 (25 µmol/L) partially reversed the protective effect of PE (Figure 4Up). These data indicate that ERKs, but not p38, are components of PE-mediated protection from cardiomyocyte apoptosis. However, the hierarchy between calcineurin and MAPK signaling pathways in regulating the hypertrophic program or protection from apoptosis is currently unknown.

Because NFAT3 acts downstream of calcineurin in mediating cardiac hypertrophy,21 we investigated the ability of NFAT3 to protect cardiomyocytes from apoptosis induced by 2-deoxyglucose using an adenovirus expressing the same truncated NFAT3 cDNA that promoted cardiac hypertrophy in transgenic mice.21 AdNFAT3 infection partially protected cardiomyocytes from 2-deoxyglucose–induced apoptosis (Figure 5Down). These data suggest that NFAT3 is partially responsible for mediating the antiapoptotic effects of calcineurin activation in cardiomyocytes. However, AdNFAT3 infection was not as protective as AdCnA infection, suggesting that calcineurin provides protection by additional mechanisms.



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Figure 5. AdNFAT3 infection imparts partial protection from 2-deoxyglucose–induced TUNEL labeling of cardiomyocytes. Data are from 3 independent experiments performed in duplicate. *P<0.05 vs Adßgal; {dagger}P<0.05 vs AdCnA.

To this end, we examined the levels or activation state of multiple proteins known to promote cell survival. Although the phosphorylation state of BAD and the protein amounts of Bcl-2, Bcl-xL, and Bax proteins were unchanged, the Akt/PKB phosphorylation state was found to be enhanced. Akt was previously shown to directly promote cell viability through multiple mechanisms.32 33 34 We observed that PE stimulation for 3 hours was sufficient to induce Akt phosphorylation, but that inhibition of calcineurin with cyclosporine blocked Akt phosphorylation (Figure 6ADown). Augmentation of intracellular calcium levels with the ionophore A23187, a known inducer of calcineurin, stimulated Akt phosphorylation after 6 hours (Figure 6BDown). This induction of Akt after 6 hours by A23187 was blocked by cyclosporine, indicating that calcineurin was partially regulating Akt activation. Finally, transgenic mice expressing the activated calcineurin cDNA were analyzed for Akt activation. We observed a significant increase in Akt phosphorylation in calcineurin-transgenic hearts at 8 weeks, but not 8 days (Figure 6CDown). The increase in Akt phosphorylation at 8 weeks is associated with the onset of heart failure in these mice, whereas day 8 is prehypertrophic. Collectively, these data indicate that Akt is partially responsible for mediating the antiapoptotic effects of calcineurin in vitro and in vivo.



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Figure 6. Akt phosphorylation is associated with calcineurin activation. A, Western blot showing phosphorylated and nascent Akt protein from PE-treated cardiomyocytes. At 3 hours, a significant increase in Akt phosphorylation is noted, which is prevented with cyclosporine. B, Western blot for Akt from A23187-treated cardiomyocytes shows specific phosphorylation after 6 hours, which is sensitive to cyclosporine. C, Western blot of protein extracts from 8-week-old, failing calcineurin-transgenic hearts shows increased Akt phosphorylation, without a change in total Akt protein. CsA indicates cyclosporine; TG, transgenic; NTG, nontransgenic.

Calcineurin-Transgenic Mice Show Dilated Heart Failure
We have previously reported that transgenic mice expressing the constitutively active calcineurin cDNA in the heart develop profound hypertrophy characterized by a 2- to 3-fold increase in heart-to-body-weight ratio, which rapidly progresses to dilated heart failure.21 To analyze this effect in more detail, we performed echocardiography on 8-week-old transgenic mice (mouse line 37). A representative M-mode tracing demonstrates the severity of heart failure in these mice (Figure 7Down). Fractional shortening was consistently reduced by 70% to 80% by 8 weeks of age compared with nontransgenic littermate controls (S.A. Witt, T.R. Kimball, and J.D. Molkentin, unpublished results, 1998).



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Figure 7. Echocardiography of 8-week-old wild-type (left) and calcineurin-transgenic (right) mice. An M-mode representation is shown that clearly demonstrates the failed phenotype of calcineurin-transgenic hearts (reduced fractional shortening and dilation of the left ventricle). Approximately 1.5 seconds of recording time is shown. LV indicates left ventricle; RV, right ventricle; and TG, transgenic.

Absence of Increased Cardiomyocyte TUNEL Staining in Calcineurin-Transgenic Hearts
Because dilated heart failure is associated with increased cardiomyocyte apoptosis, we reasoned that failing calcineurin-transgenic hearts would show enhanced cell death. However, an exhaustive histologic analysis of TUNEL-stained 8-week-old hearts failed to reveal a significant difference in cardiomyocyte TUNEL reactivity between transgenic and wild-type mice (Figure 8ADown). In contrast, calcineurin-transgenic hearts showed a statistically significant increase in nonmyocyte TUNEL staining, likely because of the increase in interstitial space associated with mild edema, immune cell infiltration, and heart failure (P<0.05) (Figure 8BDown). A typical TUNEL-stained histological section from a calcineurin-transgenic and a nontransgenic heart demonstrates predominantly interstitial cell reactivity (blue) (Figures 8CDown and 8DDown).



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Figure 8. Quantification of TUNEL labeling of histological sections from 8-week-old calcineurin-transgenic or nontransgenic hearts. Four hearts of each genotype were sectioned (6 µmol/L) for TUNEL labeling and quantification. A, Number of TUNEL-positive cells per 10 000 cells. B, Number of TUNEL-positive interstitial cells per entire heart cross-section without atria. C and D, Representative histological sections from a nontransgenic (C) and a calcineurin-transgenic (D) heart processed for TUNEL staining (blue areas, arrowheads). CnA indicates calcineurin; TG, transgenic. *P<0.05 vs nontransgenic.

Calcineurin Protects Against Apoptosis Occurring After Ischemia/Reperfusion
To test the hypothesis that activated calcineurin might protect cardiomyocytes from apoptosis in vivo, calcineurin-transgenic and wild-type hearts were subjected to ischemia/reperfusion–induced cell death. Temporary occlusion of the left coronary artery followed by reperfusion is known to potently induce cardiomyocyte apoptosis.17 35 Cardiac ischemia followed by reperfusion in nontransgenic control mice produced substantial DNA laddering, indicating activation of apoptosis (Figure 9Down). However, ischemia/reperfusion of calcineurin-transgenic hearts demonstrated substantial protection against typical DNA fragmentation, indicating that calcineurin-mediated hypertrophy protects cardiomyocytes from apoptotic stimuli in vivo.



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Figure 9. Ischemia/reperfusion (I/R) in vivo induces significant DNA laddering in wild-type (WT) but not calcineurin-transgenic (TG) mouse hearts. Wild-type and calcineurin-transgenic mice were subjected to sham operation or 45 minutes of cardiac ischemia followed by 4 hours of reperfusion (I/R). Genomic DNA (40 µg) isolated from each heart was size-fractionated on a 1.4% agarose gel containing ethidium bromide. Total number of mice studied was as follows: WT-Sham, n=4; WT-I/R, n=6; TG-Sham, n=4; and TG-I/R, n=7.

Cyclosporine Administration Does Not Induce Apoptosis in Gq{alpha}-transgenic Mice
The observation that calcineurin protects cardiomyocytes from apoptosis in vivo might suggest that cyclosporine would be proapoptotic. To investigate this possibility in vivo, we treated Gq{alpha}-transgenic mice with cyclosporine for 8 weeks. Gq{alpha}-transgenic mice were chosen because they are uniquely sensitized to apoptotic stimulation such that increased transgene number or the postpartum period induces substantial apoptosis.7 However, hearts from cyclosporine-treated Gq{alpha}-transgenic mice, or wild-type hearts, did not demonstrate increased TUNEL labeling (Figure 10Down). The quantification of TUNEL labeling was performed in a blinded manner. As a positive control for increased TUNEL labeling, reactivity was quantified in a single Gq{alpha} heart from an aortic-banded animal. Aortic banding is a potent stimulus for apoptosis in Gq{alpha}-transgenic mice (Figure 10Down) (J. Ross and G.W. Dorn II, unpublished data, 1999). Collectively, these data indicate that cyclosporine is not sufficient to induce apoptosis in vivo or in vitro. This suggests that, whereas calcineurin activation protects myocytes from cell death, its inhibition is not sufficient to induce apoptosis in vivo.



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Figure 10. Cyclosporine administration to Gq{alpha}-transgenic mice does not induce TUNEL labeling. Gq{alpha}-transgenic mice were treated with cyclosporine at 20 mg/kg per day for 8 weeks, after which the hearts were collected for histologic TUNEL labeling. Cyclosporine did not induce TUNEL labeling in transgenic or wild-type hearts, whereas aortic banding (positive control) induced abundant labeling. Three animals were characterized in each treatment group, whereas only 1 aortic-banded control was examined. NTG indicates nontransgenic. *P<0.05 vs Gq{alpha} saline.


*    Discussion
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*Discussion
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In this study, we provide multiple lines of evidence that calcineurin protects cardiomyocytes from apoptosis both in vivo and in vitro. First, apoptosis induced by ischemia/reperfusion injury was largely rescued in calcineurin-transgenic hearts compared with nontransgenic hearts. Second, AdCnA infection protected cardiomyocytes in culture from TUNEL labeling, caspase activation, and DNA laddering. Third, direct inhibition of endogenous calcineurin either with cyclosporine or with a noncompetitive peptide inhibitor reversed the antiapoptotic effects of PE stimulation. We demonstrate calcineurin-dependent protection from apoptosis both in vivo and in vitro either through expression of an activated calcineurin mutant or by activation of endogenous calcineurin. Taken together, these data establish a role for calcineurin signaling in protecting myocardial cells from apoptotic insults. These results also establish a mouse model of dilated heart failure that is independent of apoptosis.

Inspection of the literature suggests that humans in dilated heart failure and many animal models that mimic human disease have increased numbers of apoptotic myocytes (reviewed in Reference 3636 ). These cumulative studies have suggested a causal relationship between the onset of apoptosis and progression of heart failure. Calcineurin-transgenic mice develop dramatic concentric hypertrophy (3-fold increase in heart size) that transitions to dilated heart failure by 8 weeks of life. Despite this phenotype, dilated and failing calcineurin-transgenic hearts do not show increased TUNEL labeling, but instead show resistance to ischemia/reperfusion–induced DNA laddering. This predicts that calcineurin-transgenic mice are a nonapoptotic model of heart failure. However, the causality of heart failure in calcineurin-transgenic mice is likely linked to other pathologies. First, the extreme degree of cardiac hypertrophy in calcineurin-transgenic mice is predicted to negatively impact function based on geometric constraints. Second, the profound hypertrophy in calcineurin mice is also associated with bradycardia and arrhythmia (Figure 7Up, data not shown). Third, calcineurin-transgenic hearts show considerable interstitial cell fibrosis.21 Finally, the default response of the myocardium when unable to hypertrophy further may simply be to dilate. It is likely that these factors, in the absence of apoptosis, are sufficient to induce heart failure.

The relationship between hypertrophic signaling pathways and the regulation of apoptosis is rather complex. Whereas some studies have shown antiapoptotic effects associated with various hypertrophic stimuli, other studies have shown induction of apoptosis by certain reactive signaling pathways. Among these proapoptotic pathways are the heterotrimeric GTP-binding proteins Gq{alpha} and Gs{alpha}.7 8 Similarly, cardiomyocyte apoptosis has been shown to be augmented by p38{alpha}, ß-adrenergic receptors, NO synthase activity, atrial natriuretic peptide, tumor necrosis factor-{alpha}, angiotensin II, overexpression of the transcription factor Id, and p53.9 10 11 12 13 15 37 38 39 Taken together, it appears that the regulation of cardiac myocyte apoptosis in the hypertrophied myocardium is complex and depends on the relative activation pattern of distinct signaling pathways, with Gq{alpha}, Gs{alpha}, p53, and p38{alpha} stimulation promoting cardiomyocyte death and calcineurin, NFAT3, p38ß, IGF-1/phosphatidylinositol 3-kinase/Akt, and ERK activation promoting cardiomyocyte survival.

It is difficult to dissect the direct mechanisms whereby calcineurin protects myocytes from apoptosis, because calcineurin activation also induces significant hypertrophy, which itself may afford protection. Previous studies have demonstrated that certain hypertrophic agonists or activation of certain reactive signaling pathways provides protection from apoptosis.14 15 16 17 18 19 20 Although PE, endothelin-1, cardiotrophin-1 (gp130), IGF-1, ERK activation, and p38ß activation have all been shown to be protective, a correlation with cell death–regulatory pathways (caspases or death receptors) or with proteins that regulate mitochondrial integrity (Bcl-2) has not been described.14 15 16 17 18 19 20 The direct mechanisms whereby certain reactive signaling pathways protect cardiomyocytes from apoptosis are largely uncharacterized. Similarly, calcineurin-transgenic hearts and AdCnA-infected cardiomyocytes did not demonstrate a perturbation in the Bcl-2–to–Bax ratio or the Bcl-xL–to–Bax ratio. However, one mechanism whereby calcineurin signaling directly protects cardiac myocytes is through activation of PKB/Akt. Akt is known to downregulate caspase-9 activation, phosphorylate BAD, and directly activate nuclear factor {kappa}B to promote cell survival.32 33 34 Although we have demonstrated that Akt activation is associated with calcineurin-induced hypertrophy, it is not clear how this activation occurs. However, it is likely that calcineurin signaling first activates the hypertrophic program, which then indirectly leads to Akt activation, providing protection from cell death. NFAT3 adenoviral gene transfer also provided partial protection from 2-deoxyglucose–induced TUNEL labeling, suggesting an additional mechanism whereby calcineurin provides protection. However, the direct mechanism whereby NFAT3 provides protection from cell death is unknown, but it can be speculated that protection is linked to the hypertrophic response itself.

The observation that calcineurin protects cardiomyocytes from apoptosis might also suggest that cyclosporine treatment could promote myocardial cell apoptosis in vivo. However, patients on chronic cyclosporine therapy have not been reported to be at increased risk for heart failure, although an increased propensity toward hypertrophy has been described.40 We directly addressed this issue experimentally by treating a mouse model that is prone to apoptosis (Gq{alpha}) with cyclosporine at 20 mg/kg per day for 8 weeks.7 Characterization of TUNEL labeling did not reveal an induction of cell death in wild-type or Gq{alpha}-transgenic mice with cyclosporine, yet aortic banding induced a profound increase (Figure 10Up, J. Ross and G.W. Dorn, unpublished data, 1999). In other cell types, cyclosporine has been shown to inhibit apoptosis by preventing mitochondrial pore transition, which is regulated by cyclophilin-D (reviewed in Reference 4141 ). These data suggests that cyclosporine has diverse intracellular effects, some of which protect cells and others of which may be proapoptotic. Indeed, calcineurin was recently shown to either protect cells from apoptosis or to directly induce apoptosis depending on cross talk between other intracellular signaling pathways.42

Calcineurin has been shown to promote viability in multiple cell types by suppressing apoptosis,43 44 45 46 whereas other studies have shown that calcineurin can activate apoptosis in disparate cell types.47 48 49 50 These differing accounts suggest that the role of calcineurin in controlling cell viability is complex and integrated with other signaling pathways. In cardiomyocytes, it is likely that calcineurin functions in concert with other reactive signaling pathways to achieve a balanced activation of cellular hypertrophy, which is beneficial to cellular viability.


*    Acknowledgments
 
This work was supported by NIH Grants HL-69562 and HL-62927, a Scholar Award from the Pew Foundation, a local American Heart Association affiliate grant-in-aid (to J.D.M.), and NIH Grants HL60665 and HL61550 (to R.N.K.). H.W.L. was supported by NIH Training Grant T32 ES07051. We thank the veterinary staff at Children’s Hospital Medical Center for excellent technical assistance.

Received July 6, 1999; accepted November 17, 1999.


*    References
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*References
 
1. Itoh G, Tamura J, Suzuki M, Suzuki Y, Ikeda H, Koike M, Nomura M, Jie T, Ito K. DNA fragmentation of human infarcted myocardial cells demonstrated by the nick end labeling method and DNA agarose gel electrophoresis. Am J Pathol. 1995;146:1325–1331.[Abstract]

2. Saraste A, Pulkki K, Kallajoki M, Henriksen K, Parvinen KM, Voipio-Pulkki LM. Apoptosis in human acute myocardial infarction. Circulation. 1997;95:320–323.[Abstract/Free Full Text]

3. Narula J, Haider N, Virmani R, DiSalvo TG, Kolodgie FD, Hajjar RJ, Schmidt U, Semigran MJ, Dec GW, Khaw BA. Apoptosis in myocytes in end-stage heart failure. N Engl J Med. 1996;335:1182–1189.[Abstract/Free Full Text]

4. Olivetti G, Abbi R, Quaini F, Kajstura J, Cheng W, Nitahara JA, Quaini E, Di Loreto C, Beltrami CA, Krajewski S, Reed JC, Anversa P. Apoptosis in the failing human heart. N Engl J Med. 1997;336:1131–1141.[Abstract/Free Full Text]

5. MacLellan WR, Schneider MD. Death by design: programmed cell death in cardiovascular biology and disease. Circ Res. 1997;81:137–144.[Abstract/Free Full Text]

6. Haunstetter A, Izumo S. Apoptosis: basic mechanisms and implications for cardiovascular disease. Circ Res. 1998;82:1111–1129.[Free Full Text]

7. Adams JW, Sakata Y, Davis MG, Sah VP, Wang Y, Liggett SB, Chien KR, Brown JH, Dorn GW II. Enhanced G{alpha}q signaling: a common pathway mediates cardiac hypertrophy and apoptotic heart failure. Proc Natl Acad Sci U S A.. 1998;95:10140–10145.[Abstract/Free Full Text]

8. Geng YJ, Ishikawa Y, Vatner DE, Wagner TE, Bishop SP, Vatner SF, Homcy CJ. Apoptosis of cardiac myocytes in Gs{alpha} transgenic mice. Circ Res. 1999;84:34–42.[Abstract/Free Full Text]

9. Shizukida Y, Buttrick PM, Geenen DL, Borczuk AC, Kitsis RN, Sonnenblick EH. ß-Adrenergic stimulation causes cardiocyte apoptosis: influence of tachycardia and hypertrophy. Am J Physiol. 1998;275:H961–H968.[Abstract/Free Full Text]

10. Leri A, Claudio PP, Li Q, Wang X, Reiss K, Wang S, Malhotra A, Kajstura J, Anversa P. Stretch-mediated release of angiotensin II induces myocytes apoptosis by activating p53 that enhances the local renin-angiotensin system and decreases the Bcl-2-to-Bax protein ratio in the cell. J Clin Invest. 1998;101:1326–1342.[Medline] [Order article via Infotrieve]

11. Leri A, Liu Y, Claudio PP, Kajstura J, Wang X, Wang S, Kang P, Malhotra A, Anversa P. Insulin-like growth factor-1 induces Mdm2 and down-regulates p53, attenuating the myocyte renin-angiotensin system and stretch-mediated apoptosis. Am J Pathol. 1999;154:567–580.[Abstract/Free Full Text]

12. Wu CF, Bishopric NH, Pratt RE. Atrial natriuretic peptide induces apoptosis in neonatal rat cardiac myocytes. J Biol Chem. 1997;272:14860–14866.[Abstract/Free Full Text]

13. Kubota T, McTiernan CF, Frye CS, Slawson SE, Lemster BH, Koretsky AP, Demetris AJ, Feldman AM. Dilated cardiomyopathy in transgenic mice with cardiac-specific overexpression of tumor necrosis factor-alpha. Circ Res. 1997;81:627–635.[Abstract/Free Full Text]

14. Zechner D, Craig R, Hanford DS, McDonough PM, Sabbadini RA, Glembotski CC. MKK6 activates myocardial cell NF-{kappa}B and inhibits apoptosis in a p38 mitogen-activated protein kinase-dependent manner. J Biol Chem. 1998;273:8232–8239.[Abstract/Free Full Text]

15. Wang Y, Huang S, Sah VP, Ross J Jr, Heller Brown J, Han J, Chien KR. Cardiac muscle cell hypertrophy and apoptosis induced by distinct members of the p38 mitogen-activated protein kinase family. J Biol Chem. 1998;273:2161–2168.[Abstract/Free Full Text]

16. Sheng Z, Knowlton K, Chen J, Hoshijima M, Brown JH, Chien KR. Cardiotrophin 1 (CT-1) inhibition of cardiac myocyte apoptosis via a mitogen-activated protein kinase-dependent pathway: divergence from downstream CT-1 signals for myocardial cell hypertrophy. J Biol Chem. 1997;272:5783–5791.[Abstract/Free Full Text]

17. Li Q, Li B, Wang X, Leri A, Jana KP, Liu Y, Kajstura J, Baserga R, Anversa P. Overexpression of insulin-like growth factor-1 in mice protects from myocyte death after infarction, attenuating ventricular dilatation, wall stress, and cardiac hypertrophy. J Clin Invest. 1997;100:1991–1999.[Medline] [Order article via Infotrieve]

18. Parrizas M, Saltiel AR, LeRoith D. Insulin-like growth factor 1 inhibits apoptosis using the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways. J Biol Chem. 1997;272:154–161.[Abstract/Free Full Text]

19. Wang L, Ma W, Markovich R, Chen J-W, Wang PH. Regulation of cardiomyocyte apoptotic signaling by insulin-like growth factor-1. Circ Res. 1998;83:516–522.[Abstract/Free Full Text]

20. Zhu H, Qi M, McElwee-Witmer SA, Merkel-Jordan L, Perrone MH, Clark KL, Zilberstein A. The relationship between hypertrophy and apoptosis in cultured neonatal ventricular cardiomyocytes. Circulation. 1998;96(suppl I):I-347. Abstract.

21. Molkentin JD, Lu JR, Antos CL, Markham B, Richardson J, Robbins J, Grant SR, Olson EN. A calcineurin-dependent transcriptional pathway for cardiac hypertrophy. Cell. 1998;93:215–228.[Medline] [Order article via Infotrieve]

22. Bialik S, Geenen DL, Sasson IE, Cheng R, Horner JW, Evans SM, Lord EM, Koch CJ, Kitsis RN. Myocyte apoptosis during acute myocardial infarction in the mouse localizes to hypoxic regions but occurs independently of p53. J Clin Invest. 1997;100:1363–1372.[Medline] [Order article via Infotrieve]

23. De Windt LJ, Willemsen PHM, Pöpping S, Van der Vusse GJ, Reneman RS, Van Bilsen M. Cloning and cellular distribution of a group II phospholipase A2 expressed in the heart. J Mol Cell Cardiol. 1997;8:2095–2106.

24. Gomez-Foix AM, Coats WS, Baque S, Alam T, Gerard RD, Newgard CB. Adenovirus-mediated transfer of the muscle glycogen phosphorylase gene into hepatocytes confers altered regulation of glycogen metabolism. J Biol Chem. 1992;267:25129–25134.[Abstract/Free Full Text]

25. Sun L, Youn HD., Loh C, Stolow M, He W, Liu JO. Cabin 1, a negative regulator for calcineurin signaling in T lymphocytes. Immunity. 1998;8:703–711.[Medline] [Order article via Infotrieve]

26. Lai MM, Burnett PE, Wolosker H, Blackshaw S, Snyder SH. Cain, a novel physiologic protein inhibitor of calcineurin. J Biol Chem. 1998;273:18325–18331.[Abstract/Free Full Text]

27. Mittereder N, March KL, Trapnell BC. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J Virol. 1996;70:7498–7509.[Abstract]

28. Yue TL, Wang C, Romanic AM, Kikly K, Keller P, DeWolf WE Jr, Hart TK, Thomas HC, Storer B, Gu JL, Wang X, Feuerstein GZ. Staurosporine-induced apoptosis in cardiomyocytes: a potential role of caspase-3. J Mol Cell Cardiol. 1998;30:495–507.[Medline] [Order article via Infotrieve]

29. Malhotra R, Brosius FC III. Glucose uptake and glycolysis reduce hypoxia-induced apoptosis in cultured neonatal rat cardiac myocytes. J Biol Chem. 1999;274:12567–12575.[Abstract/Free Full Text]

30. Bialik S, Cryns VL, Drincic A, Miyata S, Wollowick AL, Srinivasan A, Kitsis RN. The mitochondrial apoptotic pathway is activated by serum and glucose deprivation in cardiac myocytes. Circ Res. 1999;85:403–414.

31. Batiuk TD, Urmson J, Vincent D, Yatscoff RW, Halloran PF. Quantitating immunosuppression. Transplantation. 1996;61:1618–1624.[Medline] [Order article via Infotrieve]

31. Taigen T, De Windt LJ, Lim HW, Molkentin JD. Targeted inhibition of calcineurin prevents agonist-induced cardiomyocyte hypertrophy. Proc Natl Acad Sci U S A. In press.

32. del Peso L, Gonzalez-Garcia M, Herrera R, Nunez G. Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science. 1997;278:687–689.[Abstract/Free Full Text]

33. Cardone MH, Roy N, Stennicke HR, Salvesen GS, Franke TF, Stanbridge E, Frisch S, Reed JC. Regulation of cell death protease caspase-9 by phosphorylation. Science. 1998;282:1381–1321.

34. Romashkova JA, Makarov SS. NF-kappaB is a target of AKT in anti-apoptotic PDGF signaling. Nature. 1999;401:86–90.[Medline] [Order article via Infotrieve]

35. Reiss K, Kajstura J, Zhang X, Li P, Olivetti G, Anversa P. Acute myocardial infarction leads to upregulation of the IGF-1 autocrine system, DNA replication, and nuclear mitotic division in the remaining viable cardiac myocytes. Exp Cell Res. 1994;213:463–472.[Medline] [Order article via Infotrieve]

36. Haunstetter A, Izumo S. Apoptosis: basic mechanisms and implications for cardiovascular disease. Circ Res. 1998;82:1111–1129.

37. Ing DJ, Dzau VJ, Webster KA, Bishopric NH. Modulation of cytokine-induced cardiac myocyte apoptosis by nitric oxide, Bax, and Bcl-x. Circ Res. 1999;84:21–33.[Abstract/Free Full Text]

38. Koglin J, Granville DJ, Glysing-Jensen T, Mudgett JS, Carthy CM, McManus BM, Russell ME. Attenuated acute cardiac rejection in NOS2–/– recipients correlates with reduced apoptosis. Circulation. 1999;99:836–842.[Abstract/Free Full Text]

39. Bryant D, Becker L, Richardson J, Shelton J, Franco F, Peshock R, Thompson M, Giroir B. Cardiac failure in transgenic mice with myocardial expression of tumor necrosis factor-alpha. Circulation. 1998;97:1375–1381.[Abstract/Free Full Text]

40. Ventura HO, Malik FS, Mehra MR, Stapelton DD, Smart FW. Mechanisms of hypertension in cardiac transplantation and the role of cyclosporine. Curr Opin Cardiol. 1997;12:375–381.[Medline] [Order article via Infotrieve]

41. Crompton M. The mitochondrial permeability pore and its role in cell death. Biochem J. 1999;341:233–249.

42. Lotem J, Kama R, Sachs L. Suppression or induction of apoptosis by opposing pathways downstream from calcium-activated calcineurin. Proc Natl Acad Sci U S A.. 1999;96:12016–12020.[Abstract/Free Full Text]

43. Asada A, Zhao Y, Kondo S, Iwata M. Induction of thymocyte apoptosis by Ca2+-independent protein kinase C (nPKC) activation and its regulation by calcineurin activation. J Biol Chem. 1998;273:28392–28398.[Abstract/Free Full Text]

44. Lotem J, Sachs L. Different mechanisms for suppression of apoptosis by cytokines and calcium mobilizing compounds. Proc Natl Acad Sci U S A.. 1998;95:4601–4606.[Abstract/Free Full Text]

45. Davis PK, Dudek SM, Johnson GV. Select alterations in protein kinases and phosphatases during apoptosis of differentiated PC12 cells. J Neurochem. 1997;68:2338–2347.[Medline] [Order article via Infotrieve]

46. Zhao Y, Tozawa Y, Iseki R, Mukai M, Iwata M. Calcineurin activation protects T cells from glucocorticoid-induced apoptosis. J Immunol. 1995;154:6346–6354.[Abstract]

47. Shibasaki F, McKeon F. Calcineurin functions in Ca(2+)-activated cell death in mammalian cells. J Cell Biol. 1995;131:735–743.[Abstract/Free Full Text]

48. Ankarcrona M, Dypbukt JM, Orrenius S, Nicotera P. Calcineurin and mitochondrial function in glutamate-induced neuronal cell death. FEBS Lett. 1996;394:321–324.[Medline] [Order article via Infotrieve]

49. Toth R, Szegezdi E, Molnar G, Lord JM, Fesus L, Szondy Z. Regulation of cell surface expression of Fas (CD95) ligand and susceptibility to Fas (CD95)-mediated apoptosis in activation-induced T cell death involves calcineurin and protein kinase C, respectively. Eur J Immunol. 1999;29:383–393.[Medline] [Order article via Infotrieve]

50. Denecker G, Vandenabeele P, Grooten J, Penning LC, Declercq W, Beyaert R, Buurman WA, Fiers W. Differential role of calcium in tumour necrosis factor-mediated apoptosis and secretion of granulocyte-macrophage colony-stimulating factor in a T cell hybridoma. Cytokine. 1997;9:631–638.[Medline] [Order article via Infotrieve]




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Caspase Inhibition Protects against Reovirus-Induced Myocardial Injury In Vitro and In Vivo
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Myocyte hypertrophy and apoptosis: a balancing act
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Cardiovasc ResHome page
J. Tongers, B. Fiedler, D. Konig, T. Kempf, G. Klein, J. Heineke, T. Kraft, S. Gambaryan, S. M Lohmann, H. Drexler, et al.
Heme oxygenase-1 inhibition of MAP kinases, calcineurin/NFAT signaling, and hypertrophy in cardiac myocytes
Cardiovasc Res, August 15, 2004; 63(3): 545 - 552.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
P. M. Kang, P. Yue, Z. Liu, O. Tarnavski, N. Bodyak, and S. Izumo
Alterations in apoptosis regulatory factors during hypertrophy and heart failure
Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H72 - H80.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
T. Kawamura, K. Ono, T. Morimoto, M. Akao, E. Iwai-Kanai, H. Wada, N. Sowa, T. Kita, and K. Hasegawa
Endothelin-1-Dependent Nuclear Factor of Activated T Lymphocyte Signaling Associates With Transcriptional Coactivator p300 in the Activation of the B Cell Leukemia-2 Promoter in Cardiac Myocytes
Circ. Res., June 11, 2004; 94(11): 1492 - 1499.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
D. J. Lips, O. F. Bueno, B. J. Wilkins, N. H. Purcell, R. A. Kaiser, J. N. Lorenz, L. Voisin, M. K. Saba-El-Leil, S. Meloche, J. Pouyssegur, et al.
MEK1-ERK2 Signaling Pathway Protects Myocardium From Ischemic Injury In Vivo
Circulation, April 27, 2004; 109(16): 1938 - 1941.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. A. Kaiser, O. F. Bueno, D. J. Lips, P. A. Doevendans, F. Jones, T. F. Kimball, and J. D. Molkentin
Targeted Inhibition of p38 Mitogen-activated Protein Kinase Antagonizes Cardiac Injury and Cell Death Following Ischemia-Reperfusion in Vivo
J. Biol. Chem., April 9, 2004; 279(15): 15524 - 15530.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
O. F. Bueno, D. J. Lips, R. A. Kaiser, B. J. Wilkins, Y.-S. Dai, B. J. Glascock, R. Klevitsky, T. E. Hewett, T. R. Kimball, B. J. Aronow, et al.
Calcineurin A{beta} Gene Targeting Predisposes the Myocardium to Acute Ischemia-Induced Apoptosis and Dysfunction
Circ. Res., January 9, 2004; 94(1): 91 - 99.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
B. J. Wilkins, Y.-S. Dai, O. F. Bueno, S. A. Parsons, J. Xu, D. M. Plank, F. Jones, T. R. Kimball, and J. D. Molkentin
Calcineurin/NFAT Coupling Participates in Pathological, but not Physiological, Cardiac Hypertrophy
Circ. Res., January 9, 2004; 94(1): 110 - 118.
[Abstract] [Full Text] [PDF]


Home page
Recent Prog Horm ResHome page
T. Zhang, S. Miyamoto, and J. H. Brown
Cardiomyocyte Calcium and Calcium/Calmodulin-dependent Protein Kinase II: Friends or Foes?
Recent Prog. Horm. Res., January 1, 2004; 59(1): 141 - 168.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
J. W. Ryder, R. Bassel-Duby, E. N. Olson, and J. R. Zierath
Skeletal Muscle Reprogramming by Activation of Calcineurin Improves Insulin Action on Metabolic Pathways
J. Biol. Chem., November 7, 2003; 278(45): 44298 - 44304.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. B. Vega, R. Bassel-Duby, and E. N. Olson
Control of Cardiac Growth and Function by Calcineurin Signaling
J. Biol. Chem., September 26, 2003; 278(39): 36981 - 36984.
[Full Text] [PDF]


Home page
J. Virol.Home page
S. Pati, J. S. Foulke Jr., O. Barabitskaya, J. Kim, B. C. Nair, D. Hone, J. Smart, R. A. Feldman, and M. Reitz
Human Herpesvirus 8-Encoded vGPCR Activates Nuclear Factor of Activated T Cells and Collaborates with Human Immunodeficiency Virus Type 1 Tat
J. Virol., May 15, 2003; 77(10): 5759 - 5773.
[Abstract] [Full Text] [PDF]


Home page
Eur Heart JHome page
D. J. Lips, L. J. deWindt, D. J.W. van Kraaij, and P. A. Doevendans
Molecular determinants of myocardial hypertrophy and failure: alternative pathways for beneficial and maladaptive hypertrophy
Eur. Heart J., May 2, 2003; 24(10): 883 - 896.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
W. T. Pu, Q. Ma, and S. Izumo
NFAT Transcription Factors Are Critical Survival Factors That Inhibit Cardiomyocyte Apoptosis During Phenylephrine Stimulation In Vitro
Circ. Res., April 18, 2003; 92(7): 725 - 731.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
M. R. Sayen, A. B. Gustafsson, M. A. Sussman, J. D. Molkentin, and R. A. Gottlieb
Calcineurin transgenic mice have mitochondrial dysfunction and elevated superoxide production
Am J Physiol Cell Physiol, February 1, 2003; 284(2): C562 - C570.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
J. R. Gonzalez-Juanatey, M. J. Iglesias, C. Alcaide, R. Pineiro, and F. Lago
Doxazosin Induces Apoptosis in Cardiomyocytes Cultured In Vitro by a Mechanism That Is Independent of {alpha}1-Adrenergic Blockade
Circulation, January 7, 2003; 107(1): 127 - 131.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Renal Physiol.Home page
J. L. Gooch, J. L. Barnes, S. Garcia, and H. E. Abboud
Calcineurin is activated in diabetes and is required for glomerular hypertrophy and ECM accumulation
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F144 - F154.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
E. van Rooij, P. A. Doevendans, C. C. de Theije, F. A. Babiker, J. D. Molkentin, and L. J. De Windt
Requirement of Nuclear Factor of Activated T-cells in Calcineurin-mediated Cardiomyocyte Hypertrophy
J. Biol. Chem., December 6, 2002; 277(50): 48617 - 48626.
[Abstract] [Full Text] [PDF]


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DiabetesHome page
Y. Pang, D. L. Hunton, P. Bounelis, and R. B. Marchase
Hyperglycemia Inhibits Capacitative Calcium Entry and Hypertrophy in Neonatal Cardiomyocytes
Diabetes, December 1, 2002; 51(12): 3461 - 3467.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
M. A. Sussman, A. McCulloch, and T. K. Borg
Dance Band on the Titanic: Biomechanical Signaling in Cardiac Hypertrophy
Circ. Res., November 15, 2002; 91(10): 888 - 898.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
O. F. Bueno and J. D. Molkentin
Involvement of Extracellular Signal-Regulated Kinases 1/2 in Cardiac Hypertrophy and Cell Death
Circ. Res., November 1, 2002; 91(9): 776 - 781.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Pathol.Home page
P A J Krijnen, R Nijmeijer, C J L M Meijer, C A Visser, C E Hack, and H W M Niessen
Apoptosis in myocardial ischaemia and infarction
J. Clin. Pathol., November 1, 2002; 55(11): 801 - 811.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
B. Fiedler, S. M. Lohmann, A. Smolenski, S. Linnemuller, B. Pieske, F. Schroder, J. D. Molkentin, H. Drexler, and K. C. Wollert
Inhibition of calcineurin-NFAT hypertrophy signaling by cGMP-dependent protein kinase type I in cardiac myocytes
PNAS, August 20, 2002; 99(17): 11363 - 11368.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. J. Donelan, G. Morfini, R. Julyan, S. Sommers, L. Hays, H. Kajio, I. Briaud, R. A. Easom, J. D. Molkentin, S. T. Brady, et al.
Ca2+-dependent Dephosphorylation of Kinesin Heavy Chain on beta -Granules in Pancreatic beta -Cells. IMPLICATIONS FOR REGULATED beta -GRANULE TRANSPORT AND INSULIN EXOCYTOSIS
J. Biol. Chem., June 28, 2002; 277(27): 24232 - 24242.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
S. E. Hardt and J. Sadoshima
Glycogen Synthase Kinase-3{beta}: A Novel Regulator of Cardiac Hypertrophy and Development
Circ. Res., May 31, 2002; 90(10): 1055 - 1063.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
B. J Wilkins and J. D Molkentin
Calcineurin and cardiac hypertrophy: Where have we been? Where are we going?
J. Physiol., May 15, 2002; 541(1): 1 - 8.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
O. F Bueno, E. van Rooij, J. D Molkentin, P. A Doevendans, and L. J De Windt
Calcineurin and hypertrophic heart disease: novel insights and remaining questions
Cardiovasc Res, March 1, 2002; 53(4): 806 - 821.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
H. FAUVEL, P. MARCHETTI, G. OBERT, O. JOULAIN, C. CHOPIN, P. FORMSTECHER, and R. NEVIERE
Protective Effects of Cyclosporin A from Endotoxin-induced Myocardial Dysfunction and Apoptosis in Rats
Am. J. Respir. Crit. Care Med., February 15, 2002; 165(4): 449 - 455.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
C. GILL, R. MESTRIL, and A. SAMALI
Losing heart: the role of apoptosis in heart disease--a novel therapeutic target?
FASEB J, February 1, 2002; 16(2): 135 - 146.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. L. Antos, T. A. McKinsey, N. Frey, W. Kutschke, J. McAnally, J. M. Shelton, J. A. Richardson, J. A. Hill, and E. N. Olson
Activated glycogen synthase-3beta suppresses cardiac hypertrophy in vivo
PNAS, January 7, 2002; (2002) 231619298.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Shizukuda and P. M. Buttrick
Protein kinase C-zeta modulates thromboxane A2-mediated apoptosis in adult ventricular myocytes via Akt
Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H320 - H327.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. L. Gooch, Y. Tang, J. M. Ricono, and H. E. Abboud
Insulin-like Growth Factor-I Induces Renal Cell Hypertrophy via a Calcineurin-dependent Mechanism
J. Biol. Chem., November 2, 2001; 276(45): 42492 - 42500.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
J. D. Molkentin
Calcineurin, Mitochondrial Membrane Potential, and Cardiomyocyte Apoptosis
Circ. Res., June 22, 2001; 88(12): 1220 - 1222.
[Full Text] [PDF]


Home page
Physiol. GenomicsHome page
B. J. ARONOW, T. TOYOKAWA, A. CANNING, K. HAGHIGHI, U. DELLING, E. KRANIAS, J. D. MOLKENTIN, and G. W. DORN II
Divergent transcriptional responses to independent genetic causes of cardiac hypertrophy
Physiol Genomics, June 6, 2001; 6(1): 19 - 28.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
L. J. De Windt, H. W. Lim, O. F. Bueno, Q. Liang, U. Delling, J. C. Braz, B. J. Glascock, T. F. Kimball, F. del Monte, R. J. Hajjar, et al.
Targeted inhibition of calcineurin attenuates cardiac hypertrophy invivo
PNAS, March 13, 2001; 98(6): 3322 - 3327.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
O. F. Bueno, L. J. De Windt, H. W. Lim, K. M. Tymitz, S. A. Witt, T. R. Kimball, and J. D. Molkentin
The Dual-Specificity Phosphatase MKP-1 Limits the Cardiac Hypertrophic Response In Vitro and In Vivo
Circ. Res., January 19, 2001; 88(1): 88 - 96.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
J. N. Muth, I. Bodi, W. Lewis, G. Varadi, and A. Schwartz
A Ca2+-Dependent Transgenic Model of Cardiac Hypertrophy : A Role for Protein Kinase C{{alpha}}
Circulation, January 2, 2001; 103(1): 140 - 147.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
S. F. Steinberg
The Cellular Actions of {beta}-Adrenergic Receptor Agonists : Looking Beyond cAMP
Circ. Res., December 8, 2000; 87(12): 1079 - 1082.
[Full Text] [PDF]


Home page
Circ. Res.Home page
J. D. Molkentin
Calcineurin and Beyond : Cardiac Hypertrophic Signaling
Circ. Res., October 27, 2000; 87(9): 731 - 738.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
P. M. Kang and S. Izumo
Apoptosis and Heart Failure : A Critical Review of the Literature
Circ. Res., June 9, 2000; 86(11): 1107 - 1113.
[Full Text] [PDF]


Home page
J. Biol. Chem.Home page
L. J. De Windt, H. W. Lim, S. Haq, T. Force, and J. D. Molkentin
Calcineurin Promotes Protein Kinase C and c-Jun NH2-terminal Kinase Activation in the Heart. CROSS-TALK BETWEEN CARDIAC HYPERTROPHIC SIGNALING PATHWAYS
J. Biol. Chem., April 28, 2000; 275(18): 13571 - 13579.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Saito, Y. Hiroi, Y. Zou, R. Aikawa, H. Toko, F. Shibasaki, Y. Yazaki, R. Nagai, and I. Komuro
beta -Adrenergic Pathway Induces Apoptosis through Calcineurin Activation in Cardiac Myocytes
J. Biol. Chem., October 27, 2000; 275(44): 34528 - 34533.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
H. W. Lim, L. New, J. Han, and J. D. Molkentin
Calcineurin Enhances MAPK Phosphatase-1 Expression and p38 MAPK Inactivation in Cardiac Myocytes
J. Biol. Chem., May 4, 2001; 276(19): 15913 - 15919.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. L. Antos, T. A. McKinsey, N. Frey, W. Kutschke, J. McAnally, J. M. Shelton, J. A. Richardson, J. A. Hill, and E. N. Olson
Activated glycogen synthase-3beta suppresses cardiac hypertrophy in vivo
PNAS, January 22, 2002; 99(2): 907 - 912.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
T. Kakita, K. Hasegawa, E. Iwai-Kanai, S. Adachi, T. Morimoto, H. Wada, T. Kawamura, T. Yanazume, and S. Sasayama
Calcineurin Pathway Is Required for Endothelin-1-Mediated Protection Against Oxidant Stress-Induced Apoptosis in Cardiac Myocytes
Circ. Res., June 22, 2001; 88(12): 1239 - 1246.
[Abstract] [Full Text] [PDF]


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