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(Circulation Research. 2009;104:514.)
© 2009 American Heart Association, Inc.

Cellular Biology

miR-1 Overexpression Enhances Ca²⁺ Release and Promotes Cardiac Arrhythmogenesis by Targeting PP2A Regulatory Subunit B56 and Causing CaMKII-Dependent Hyperphosphorylation of RyR2

Dmitry Terentyev, Andriy E. Belevych, Radmila Terentyeva, Mickey M. Martin, Geraldine E. Malana, Donald E. Kuhn, Maha Abdellatif, David S. Feldman, Terry S. Elton, Sandor Györke

From the Davis Heart and Lung Research Institute (D.T., A.E.B., R.T., M.M.M., G.E.M., D.E.K., D.S.F., T.S.E., S.G.), Ohio State University, Columbus; and Cardiovascular Research Institute (M.A.), Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, Newark.

Correspondence to Dmitry Terentyev, PhD, Department of Physiology and Cell Biology, Ohio State University, DHLRI 501, 473 West 12th Ave, Columbus, OH 43210. USA. Phone: +1 614 2923944. Fax:+1 614 247 7799. E-mail Dmitry.Terentyev{at}osumc.edu

	Abstract

Top Abstract Introduction Materials and Methods Results Discussion References

 
MicroRNAs are small endogenous noncoding RNAs that regulate protein expression by hybridization to imprecise complementary sequences of target mRNAs. Changes in abundance of muscle-specific microRNA, miR-1, have been implicated in cardiac disease, including arrhythmia and heart failure. However, the specific molecular targets and cellular mechanisms involved in the action of miR-1 in the heart are only beginning to emerge. In this study we investigated the effects of increased expression of miR-1 on excitation–contraction coupling and Ca²⁺ cycling in rat ventricular myocytes using methods of electrophysiology, Ca²⁺ imaging and quantitative immunoblotting. Adenoviral-mediated overexpression of miR-1 in myocytes resulted in a marked increase in the amplitude of the inward Ca²⁺ current, flattening of Ca²⁺ transients voltage dependence, and enhanced frequency of spontaneous Ca²⁺ sparks while reducing the sarcoplasmic reticulum Ca²⁺ content as compared with control. In the presence of isoproterenol, rhythmically paced, miR-1–overexpressing myocytes exhibited spontaneous arrhythmogenic oscillations of intracellular Ca²⁺, events that occurred rarely in control myocytes under the same conditions. The effects of miR-1 were completely reversed by the CaMKII inhibitor KN93. Although phosphorylation of phospholamban was not altered, miR-1 overexpression increased phosphorylation of the ryanodine receptor (RyR2) at S2814 (Ca²⁺/calmodulin-dependent protein kinase) but not at S2808 (protein kinase A). Overexpression of miR-1 was accompanied by a selective decrease in expression of the protein phosphatase PP2A regulatory subunit B56 involved in PP2A targeting to specialized subcellular domains. We conclude that miR-1 enhances cardiac excitation–contraction coupling by selectively increasing phosphorylation of the L-type and RyR2 channels via disrupting localization of PP2A activity to these channels.

Key Words: ryanodine receptor • miR-1 • CaMKII • PP2A • arrhythmia

	Introduction

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Cardiac contractility relies on release of Ca²⁺ from the sarcoplasmic reticulum (SR) and alterations in intracellular Ca²⁺ cycling have been implicated in different cardiac diseases, including arrhythmia and heart failure. Normally, SR Ca²⁺ release is activated by Ca²⁺ that enters the cell through voltage-dependent Ca²⁺ channels of the sarcolemma during the plateau phase of the cardiac action potential. This process, known as Ca²⁺-induced Ca²⁺ release (CICR),¹ involves the ryanodine receptor (RyR2) channels on the SR and is essential for activation of contractile filaments during myocardial contraction.² Relaxation occurs when Ca²⁺ released to the cytosol is resequestered to the SR by the phospholamban (PLB)-controlled SR Ca²⁺ ATPase (SERCA). Cardiac contractility is modulated by reversible phosphorylation of the components of SR Ca²⁺ release machinery, including the L-type Ca²⁺ channel (dihydropyridine receptor [DHPR]), RyR2, and PLB, by protein kinase (PKA),^2,3 Ca²⁺/calmodulin-dependent protein kinase (CaMKII),^4,5 and phosphatases PP1 and PP2A.^6–8 Although, in general, both PKA and CaMKII potentiate SR Ca²⁺ release and enhance contractility,² the underlying mechanisms of these effects and, in particular, the role of RyR2 phosphorylation/dephosphorylation remain highly controversial.^3,9–14

MicroRNAs are recently discovered molecules consisting of 22 nucleotides that regulate gene expression by annealing to target messenger RNAs inhibiting translation or promoting mRNA degradation.¹⁵ Of the 600 microRNAs identified in vertebrates, several, including miR-1, are muscle-specific.^16–18 miR-1 has been shown to be involved in cardiac development and apoptosis^17,18 and is reportedly upregulated in hearts from individuals with coronary artery disease¹⁹ and heart failure.²⁰ Several targets for miR-1 have been identified in the heart, including connexin 43 and Kir2.1 and miR-1–mediated reductions in expression of these proteins have been implicated in arrhythmogenesis.¹⁹ However, considering the inherent capacity of miRNAs to target a broad range of proteins, the link between miR-1 and heart failure and sudden cardiac death is far from being clear and more miR-1 targets involved in these disease states are likely remain to be identified. In the present study, we investigated the effects of increased expression of miR-1 on excitation–contraction (EC) coupling and Ca²⁺ cycling in rat ventricular myocytes using cellular electrophysiology and Ca²⁺ imaging. Our results identified a new potentially important target for miR-1 in the heart, namely the PP2A regulatory subunit B56. Through translational inhibition of this mRNA target, miR-1 causes CaMKII-dependent hyperphosphorylation of RyR2, enhances RyR2 activity, and promotes arrhythmogenic SR Ca²⁺ release.

	Materials and Methods

Top Abstract Introduction Materials and Methods Results Discussion References

 
The cellular, subcellular, and molecular effects of adenovirally mediated miR-1 overexpression were studied in isolated adult rat ventricular myocytes maintained in culture for 36 to 48 hours. Cytosolic Ca²⁺ changes were monitored using confocal microscopy, and whole cell currents and membrane potential were recorded with the patch-clamp technique. Changes in levels of RNA, protein expression and protein phosphorylation were studied using standard approaches.

An expanded Materials and Methods section can be found in the online data supplement, available at http://circres.ahajournals.org.

	Results

Top Abstract Introduction Materials and Methods Results Discussion References

 
miR-1 Stimulates I_Ca and SR Ca²⁺ Release in Cardiac Myocytes
Myocytes were infected with either an adenoviral construct for expression of miR-1 (Ad-miR-1)²¹ or a construct containing a nontranslatable DNA segment that served as control (Ad-control). As determined by RT PCR, miR-1 abundance was increased 2-fold in myocytes infected with Ad-miR-1 compared with control cells (Figure I in the online data supplement). First, we investigated the effects of overexpression of miR-1 on EC coupling in voltage clamped cardiac myocytes. Inward Ca²⁺ currents (I_Ca) and intracellular Ca²⁺ transients were simultaneously measured in cardiac myocytes depolarized to membrane potentials in the range between –40 and 60 mV (Figure 1). Overexpression of miR-1 resulted in a significant increase in the amplitude of I_Ca at membrane potentials from by –40 to 20 mV (Figure 1B). Additionally, the voltage dependence of I_Ca (I-V curve) was shifted to the left by 4 mV in miR-1 myocytes (supplemental Figure II). Despite increased I_Ca, the trigger for SR Ca²⁺ release, maximum Ca²⁺ transient amplitude was not changed by miR-1 overexpression (Figure 1A and 1B). The failure of enhanced I_Ca to increase maximum SR Ca²⁺ release is attributable to reduced SR Ca²⁺ content limiting Ca²⁺ release in miR-1 cells (see below). At the same time, the voltage dependence of Ca²⁺ transients was markedly broadened and flattened compared with control because of increased Ca²⁺ transient amplitude at small and large depolarizations (Figure 1B). Whereas the increase of Ca²⁺ transient amplitude at small depolarizing pulses can be accounted for by increased I_Ca, the increase of Ca²⁺ transients at large depolarizing steps occurred without changes in I_Ca and is indicative of enhanced responsiveness of Ca²⁺ release channels to I_Ca (ie, enhanced EC coupling gain).

View larger version (22K): [in this window] [in a new window]   Figure 1. miR-1 overexpression stimulates ICa and SR Ca2+ release in cardiac myocytes. A, Representative recordings of Ca2+ transients and ICa evoked by depolarizing steps from a holding potential of –50 to –30, 0, and +30 mV in a control myocyte and a myocyte overexpressing miR-1. The voltage steps were applied at intervals of 5 seconds. B and C, Voltage dependencies of ICa (bottom) and Ca2+ transient amplitude (top) in control (blue) and miR-1–overexpressing (red) myocytes at baseline conditions (B) and on application of 100 nmol/L ISO (C). *P<0.05, significantly different vs control (1-way ANOVA); N=5 to 7.

The effects of miR-1 overexpression on SR Ca²⁺ release were further studied by measuring spontaneous local Ca²⁺ release events, Ca²⁺ sparks, in intact quiescent myocytes loaded with Fluo-3 AM (Figure 2). Spark frequency in miR-1–overexpressing cells was significantly higher than in control cells (4.39±0.54 and 2.88±0.41 100 µm^–1 sec^–1, respectively; Figure 2A and 2C). We assessed the Ca²⁺-loading state of the SR in miR-1 versus control myocytes by application of caffeine. Judging from the amplitude of caffeine-induced Ca²⁺ transients, SR Ca²⁺ content was reduced to 75% of control in miR-1 myocytes (Figure 2B and 2D). The lowered SR Ca²⁺ content is a likely result of increased spark-mediated SR Ca²⁺ leak, and it provides an explanation for the lack of potentiation of maximum Ca²⁺ release by increased I_Ca in miR-1–overexpressing myocytes. Therefore, miR-1 caused profound changes in EC coupling, including increased I_Ca, enhanced RyR2 channel functional activity, and a reduction in the SR Ca²⁺ content.

View larger version (39K): [in this window] [in a new window]   Figure 2. miR-1 overexpression increases Ca2+ spark frequency and decreases SR Ca2+ content in intact cardiac myocytes. A and B, Representative line-scan images of Ca2+ sparks (A) and time-dependent profiles of global Ca2+ releases induced by application of 10 mmol/L caffeine (B) recorded in a control myocyte and a myocyte overexpressing miR-1 at reference conditions and after treatment with the CaMKII inhibitor KN93 (1 µmol/L). C, Averaged spark frequency for the 3 myocyte groups; n=33, 53, and 45 for control, miR-1, and miR-1 treated with KN93, respectively. D, Averaged amplitude of caffeine-induced Ca2+ transients; n=14, 14, and 8 for control, miR-1, and miR-1 treated with KN93, respectively. *P<0.05, significantly different vs control (1-way ANOVA).

The Stimulatory Effects of miR-1 on I_Ca and SR Ca²⁺ Release Are Caused by Phosphorylation of DHPRs and Ryanodine Receptors
The effects of miR-1 overexpression on I_Ca in cardiomyocytes, namely the increased I_Ca amplitude and leftward shift of I_Ca voltage dependence (Figure 1B and 1C and supplemental Figure II), are similar to the effects of β-adrenergic stimulation. To examine whether the action of miR-1 involves the same mechanisms that mediate the response to β-adrenergic stimulation, we investigated the effects of the β-adrenergic agonist isoproterenol (ISO) on I_Ca and Ca²⁺ transients in miR-1 versus control myocytes. As shown in Figure 1B and 1C, ISO increased I_Ca amplitude 2-fold in control but was virtually ineffective in miR-1 myocytes. Similarly, whereas ISO resulted in a 2-fold increase in Ca²⁺ transient amplitude in control myocytes, ISO failed to cause significant changes in the amplitude and voltage dependence of Ca²⁺ transients in miR-1 myocytes. These results could be explained by miR-1 and ISO acting through the same intracellular signaling mechanisms resulting in phosphorylation of target proteins including DHPR and RyR2.

RyR2 is phosphorylated at least at 3 different sites: at S2808 by PKA²² and possibly CaMKII²³; at S2814 by CaMKII,²² and at S2030 by PKA.¹¹ To test directly the hypothesis that miR-1 overexpression results in increased phosphorylation of RyR2 at these sites, we quantified RyR2 phosphorylation using phospho-specific antibodies. miR-1 overexpression caused a marked increase in phosphorylation at the CaMKII site S2814 while leaving phosphorylation of the PKA site S2808 unaltered. The involvement of CaMKII in RyR2 phosphorylation at S2814 was further confirmed by the ability of KN93, a CaMKII inhibitor, to prevent phosphorylation at this site in miR-1 myocytes (Figure 3A through 3C). Interestingly, ryanodine receptors were not detectibly phosphorylated at S2030 either in the control or miR-1 groups even when the myocytes were exposed to 100 nmol/L ISO (supplemental Figure III). miR-1 did not change phosphorylation of PLB at its PKA or CaMKII sites, S16 and T17, respectively (Figure 3A and 3D), but increased phosphorylation of DHPRs (Figure 3A and 3E).

View larger version (28K): [in this window] [in a new window]   Figure 3. miR-1 overexpression increases RyR2 and DHPR phosphorylation and does not affect phosphorylation of PLB. A, Representative Western blots. RyR2 phosphorylation at sites S2814 and S2808 and PLB phosphorylation at S16 and T17 was measured with phospho-specific antibodies. DHPR phosphorylation level was assessed using the Pro-Q Diamond phosphoprotein gel stain technology (Invitrogen). Total protein content (ie, RyR2, PLB, or DHPR) was measured in the same samples on a different blot and used as a control for loading. Ventricular cells isolated from 1 heart were split into 5 groups, infected with the control adenovirus or the adenovirus encoding miR-1, and kept in culture for 48 hours. Subsequently, myocytes were exposed to 100 nmol/L ISO (3 minutes) and 1 µmol/L KN93 (30 minutes), paced at 1 Hz for 1 minute, and flash frozen. B, Data pooled from 6 experiments for S2808. C, Data pooled from 7 experiments for S2814. D, Data pooled from 7 independent experiments for T17. E, Data pooled from 5 independent experiments for phospho-DHPR. *P<0.05 vs control, P<0.05 vs control+ISO (paired Student’s t test).

To test the involvement of CaMKII in the functional effects of miR-1 on SR Ca²⁺ release in myocytes, we examined the impact of KN93 on miR-1–dependent changes in Ca²⁺ cycling. KN93 reversed the effects of miR-1 on frequency of Ca²⁺ sparks and on the SR Ca²⁺ content of myocytes (Figure 2), as well as on Ca²⁺ currents and Ca²⁺ transients in voltage-clamped cells (supplemental Figure IV). Collectively, these results suggest that miR-1 overexpression results in augmented phosphorylation of DHPR and RyR2 by CaMKII, whereas the phosphorylation of PLB is unaltered.

miR-1 Inhibits Expression of the PP2A Regulatory Subunit B56
The phosphorylation-dependent effects of miR-1 on Ca²⁺ handling point to the possibility that miR-1 targets components of the phosphorylation–dephosphorylation system in myocytes. Bioinformatic sequence analysis revealed that the PP2A regulatory subunit, B56, is a potential target of miR-1 because it harbors 7-bp-long complimentary seed sequence in its 3' untranslated region (UTR) (Figure 4A). Binding of miR-1 to B56 encoding RNA was confirmed by a luciferase reporter assay (Figure 4B). Moreover, the miR-1 targeting of B56 in cardiac myocytes was confirmed by quantitative immunoblot analysis using an anti-B56 antibody (Figure 5B and 5C). Importantly, miR-1 overexpression did not appreciably change expression levels of a number of relevant Ca²⁺ and phosphorylation handling proteins, including DHPR, RyR2, SERCA2a, PLB, sodium/calcium exchanger (NCX1), calsequestrin (CASQ2), catalytic subunits of PP1 and PP2A, and CaMKII, as well as phosphodiesterases 3A and 4D. As shown in Figure 5A, expression of all of these proteins was similar in miR-1 versus control myocytes. Of note, miR-1 overexpression resulted in a decrease in expression of PKA β catalytic subunit, which is also a predicted target of miR-1. However, clearly downregulation of this kinase cannot account for the increase in phosphorylation of target proteins by miR-1 in our experiments.

View larger version (48K): [in this window] [in a new window]   Figure 4. PP2A regulatory subunit B56 is a target for silencing by miR-1. A, Complementarity between miR-1 and the putative B56 3'-UTR site targeted (985 to 991 bp downstream from the human B56 stop codon; www.targetscan.org). The putative miR-1 binding site harbored in the B56 3'-UTR is conserved across species (ie, human, mouse, rat, dog, and chicken). The binding of miR-1 to the B56 3'-UTR target site fulfills the requirement of a 7-bp seed sequence of complementarity at the miRNA end. B, CHO cells were transfected with psiCHECK or the psiCHECK-B56 luciferase reporter construct and either miR-1 or scrambled miRNA at the concentrations indicated. Twenty-four hours following transfection, luciferase activities were measured. Renilla luciferase activity was normalized to firefly luciferase activity and mean activities±SE from 5 independent experiments are shown (P<0.01 vs CHO cells transfected with psiCHECK-B56 at each concentration shown).

View larger version (53K): [in this window] [in a new window]   Figure 5. miR-1 overexpression decreases amount of PP2A regulatory subunit B56 in myocytes. A, Representative immunoblots of lysates from ventricular myocytes infected with control adenoviruses or adenoviruses encoding miR-1 prepared from 2 hearts. PP2AC indicates PP2A catalytic subunit; PP1C, PP1 catalytic subunit; PKAβC, PKA catalytic subunit β isoform; CaMKIIC, CaMKII catalytic subunit isoform; CaMKII T287, CaMKII phosphorylated at threonine 287 probed with phospho-specific antibody; PDE3A and PDE4D, phosphodiesterases types 3A and 4D, respectively; Cav1.2, 1C subunit of L-type Ca2+ channel; CASQ2, cardiac isoform of calsequestrin; SERCA2a, cardiac isoform of SR Ca2+-ATPase; NCX1, Na+/Ca2+ exchanger type 1. Forty micrograms of protein per lane was used for analysis of all proteins. There is no significant difference in the density of these proteins between the 2 groups except PKAβC. B and C, Representative immunoblot (B) and pooled data for PP2A regulatory subunit B56 in control myocytes and myocytes overexpressing miR-1 (C). Data were obtained from 10 independent experiments. *P<0.05, significantly different vs control (paired Student’s t test). D, Control (top) and miR-1–overexpressing (bottom) cardiomyocytes fixed after 48 hours in culture coimmunolabeled with B56- (left, green) and RyR2-specific antibodies (middle, red).

B56 must be in a close proximity to DHPRs and RyR2s to influence their phosphorylation state. To examine the cellular distribution of B56 with respect to RyR2 in cardiac myocytes, we performed immunostaining experiments. As demonstrated in Figure 5D, B56 shows a substantial degree of colocalization with RyR2 at the Z-lines. Thus, B56 and RyR2 (and hence DHPR) appear to coexist in specific subcellular compartments of ventricular myocytes. miR-1 overexpression reduced B56 abundance uniformly without a change in the overall distribution pattern across the cells. In accordance with the targeting role of B56, the distribution of PP2A catalytic subunit changed from localized preferentially at the Z-lines to more diffused following overexpression of miR-1 (supplemental Figure V).

β-Adrenergic Stimulation Promotes Arrhythmia in miR-1–Overexpressing Myocytes
RyR2 hyperphosphorylation by either PKA or CaMKII has been suggested to present a possible mechanism for Ca²⁺-dependent arrhythmia, including catecholaminergic polymorphic ventricular tachycardia.^24,25 To determine whether miR-1 overexpression renders myocytes prone to arrhythmogenic alterations in Ca²⁺ cycling, we examined miR-1 and control myocytes as to the occurrence of spontaneous, extrasystolic Ca²⁺ transients and associated delayed afterdepolarizations (DADs) (Figure 6 and supplemental Figure VI).^26,27 Control and miR-1 myocytes were paced in the presence or absence of ISO (100 nmol/L). In the presence of ISO, the 2 groups showed no significant differences in the amplitude of Ca²⁺ transients and SR Ca²⁺ load when paced at 1 Hz (Figure 6A and 6B). Control myocytes exposed to ISO exhibited relatively few Ca²⁺ waves and DADs. However, the number of spontaneous extrasystolic Ca²⁺ waves per second increased more than 10-fold in miR-1–overexpressing cells compared with controls (Figure 6C). Simultaneously with Ca²⁺ waves, these cells exhibited DADs (supplemental Figure VI). Remarkably, incubation of miR-1 overexpressing myocytes with 1 µmol/L KN93 completely abolished the miR-1-mediated boost in arrhythmogenic activity (Figure 6A and 6C). In the absence of ISO, spontaneous Ca²⁺ waves were only rarely observed in paced miR-1 myocytes. However, the properties of SR Ca²⁺ release in these cells were markedly altered compared to control. The amplitude of Ca²⁺ transients in miR-1 myocytes was 2-fold higher than in control (supplemental Figure VII). Additionally, miR-1 myocytes exhibited substantial spontaneous diastolic SR Ca²⁺ release, seen as Ca²⁺ sparks, much less pronounced in control cells (see insets in supplemental Figure VII). Collectively, these results suggest that miR-1 overexpression renders ryanodine receptors hyperactive even in the absence of PKA phosphorylation and that arrhythmogenic spontaneous Ca²⁺ release is linked to RyR2 phosphorylation by CaMKII rather by PKA.

View larger version (54K): [in this window] [in a new window]   Figure 6. miR-1 overexpression increases arrhythmogenic potential of myocytes undergoing repetitive stimulation in the presence of ISO. A, Representative line-scan images and corresponding time-dependent profiles of Fluo-3 fluorescence in rat ventricular myocytes infected with Ad-control and Ad-miR-1 adenoviruses. Ca transients were evoked by electric field stimulation at 1 Hz in the presence of 100 nmol/L ISO. miR-1–overexpressing myocytes displayed increased incidence of arrhythmogenic spontaneous diastolic Ca2+ waves. Incubation of miR-1–overexpressing myocytes with KN93 (1 µmol/L for 30 minutes) restored normal rhythmic activity. B, Summarized data for Ca2+ transient amplitude and SR Ca2+ content for control, miR-1–overexpressing myocytes and miR-1 myocytes treated with KN93 in the presence of 100 nmol/L ISO. The myocytes were field-stimulated at 0.5 and 1 Hz for 1 minute each then exposed 10 mmol/L caffeine. C, Averaged number of spontaneous Ca2+ waves per second in field-stimulated myocytes at 1 Hz in the presence of 100 nmol/L ISO. *P<0.05, significantly different (1-way ANOVA); number of cells studied, n=8 to 16.

	Discussion

Top Abstract Introduction Materials and Methods Results Discussion References

 
In the present study, we investigated the effects of the muscle-specific microRNA, miR-1, on EC coupling and regulation of SR Ca²⁺ release in cardiac myocytes. Our major findings are that miR-1 enhanced the functional activity of RyR2 channels and thus resulted in increased EC coupling gain, elevated diastolic SR Ca²⁺ leak, and reduced SR Ca²⁺ content and promoted arrhythmogenic disturbances in myocyte Ca²⁺ cycling. These effects were attributable to translational inhibition by miR-1 of the B56 regulatory subunit of protein phosphatase PP2A, in turn, causing hyperphosphorylation of RyR2 by CaMKII. These findings have important implications for the functional role of microRNAs and mechanisms of arrhythmogenesis in cardiac muscle.

Our conclusion that miR-1 altered myocyte Ca²⁺ signaling through disruption of site-specific PP2A phosphatase activity was supported by the following results: (1) miR-1 expression in myocytes resulted in reduced expression of B56 involved in subcellular localization of PP2A activity; (2) miR-1 increased phosphorylation of the DHPR and RyR2 without affecting phosphorylation of PLB localized in a separate cellular domain; (3) B56 colocalized with RyR2 in myocytes; and (4) The functional effects of miR-1 on EC coupling and intracellular Ca²⁺ cycling were reversed by the inhibitor of CaMKII KN93. The PP2A catalytic subunit has been shown to complex with DHPR⁷ and RyR2⁸ and is critical to dephosphorylation of these proteins following their phosphorylation by PKA and/or CaMKII.^7,8 Consistent with the role of B56 in conveying PP2A phosphatase activity to DHPR, B56 has been shown to coimmunoprecipitate with the subunit of the cardiac L-type Ca²⁺ channel (Ca_v1.2).²⁸ As for RyR2, it has been previously reported that the PP2A catalytic subunit associates with the RyR2 complex through the regulatory subunit PR130, which binds directly to RyR2 by a leucine zipper motif.²⁹ However, because PR130 does not present a target for miR-1, it is unlikely to play a role in the effects of miR-1 in our experiments. In support of the involvement of B56, it has been shown to interact with the anchoring protein, ankyrin-B,³⁰ which in turn binds to RyR2,³¹ thereby linking PP2A activity to the RyR2. Of note, myocytes from genetically altered mice lacking ankyrin-B exhibit increased propensity for spontaneous Ca²⁺ release and DADs when challenged with ISO similar to the effects of miR-1 in our study (see below for discussion). Moreover, human mutations in ankyrin-B have been linked to catecholaminergic polymorphic ventricular tachycardia: an arrhythmic disorder associated with abnormal Ca²⁺ handling and DADs.^32,33 Based on our findings, disruption of PP2A activity may contribute to, or account for, those abnormalities.

The RyR2 is phosphorylated by PKA and CaMKII at a number of different sites.^11,22,23 In our study, the alterations of RyR2 function and SR Ca release in miR-1–overexpressing myocytes appear to be caused by CaMKII rather than PKA for the following reasons. First, the potentiation of RyR2 function was associated with increased phosphorylation of RyR2 at the CaMKII site S2814 but not at the PKA site S2808, as determined by phospho-specific antibodies directed to these sites. Furthermore, the effects of miR-1 could be prevented by the CaMKII inhibitor KN93. The observed changes in myocyte Ca²⁺ handling, including increased EC coupling gain, reduced SR Ca²⁺ content, and increased SR RyR2-mediated Ca²⁺ leak are similar to those observed by others in cells overexpressing CaMKII²⁵ and are consistent with miR-1 effects being mediated by CaMKII. Thus, collectively, these results suggest that miR-1 influences myocyte Ca²⁺ signaling by regulating the spatial distribution of phosphatase PP2A activity and hence promoting RyR2 phosphorylation by CaMKII.

Additionally, phosphorylation of the L-type Ca²⁺ channel (1C and/or β subunits of DHPR) by PKA, PKC, and CaMKII is known to occur at a number of sites and cause potentiation of the L-type Ca²⁺ current.^4,7,28,34 At the present time, we do not know which of the specific phosphorylation pathways and sites are involved in modulation of I_Ca by miR-1 in our study. In principle, by targeting PP2A activity in the vicinity of the L-type Ca²⁺ channels, miR-1 would be expected to enhance phosphorylation at all sites. The failure of ISO to further stimulate the enlarged I_Ca in miR-1 cells (Figure 1) supports the possibility that PKA plays a role in mediating the effects of miR-1 on I_Ca. At the same time, the fact that CaMKII inhibition attenuates the miR-1-induced increase in I_Ca (supplemental Figure IV) points to the possibility that CaMKII might be also involved. Future studies using genetic mouse models with targeted mutations of the phosphorylation sites on the L-type Ca²⁺ channel may help to resolve this issue.

An important finding of this work is that miR-1 resulted in profound disturbances in myocyte Ca²⁺ cycling manifested as extrasystolic spontaneous Ca²⁺ releases. Spontaneous Ca²⁺ releases are arrhythmogenic as they results in DADs and early afterdepolarizations.^26,27 DADs and early afterdepolarizations in turn present triggering events for arrhythmia. Interestingly, these abnormal Ca²⁺ releases typically occurred after stimulation of the miR-1–overexpressing cells with ISO and were only rarely observed in ISO-treated control myocytes or untreated miR-1–overexpressing cells. Correlating these functional effects with the state of RyR2 phosphorylation at S2808 and S2814 revealed that disrupted Ca²⁺ cycling was associated with maximum phosphorylation at S2814 but not necessarily at S2808, and again this abnormal Ca²⁺ cycling was completely preventable by inhibition of CaMKII. The increased occurrence of global spontaneous Ca²⁺ waves and DADs in miR-1 cells in the presence of ISO is likely to be attributable to stimulation of SERCA-mediated SR Ca²⁺ uptake rather than caused by additional effects on CAMKII-phosphorylated ryanodine receptors. The stimulatory effects of CaMKII on SR Ca²⁺ release and the involvement of CaMKII in the proarrhythmic effects of miR-1 are consistent with previous studies which showed that CaMKII activation is involved in arrhythmia induction in various pathological settings including cardiac hypertrophy and heart failure.^25,35

Previously, we reported that exogenous phosphatases, including PP2A, elevate cardiac SR Ca²⁺ leak by stimulation of ryanodine receptors.¹⁰ This result is in apparent contradiction with the stimulatory effects of increased RyR2 phosphorylation described in the present study. Recently, we found that both phosphorylation and dephosphorylation can stimulate RyR2 activity, resulting in increased SR Ca²⁺ leak in cardiomyocytes.³⁶ Although we do not have a definitive explanation for these apparently contradicting results, they can be rationalized by considering that RyR2 is a multimer with multiple sets of phosphorylation sites. It is possible that RyR2 activity is lowest at intermediate phosphorylation of a certain set of sites (ie, S2814), whereas both hypo- or hyperphosphorylation of the sites leads to increased activity. It is also possible that the observed stimulatory effects of kinases and phosphatases are mediated by different sets of sites. Elucidation of the precise mechanisms of action of phosphorylation and dephosphorylation on RyR2 should await further studies.

miR-1 levels have been shown to increase in human cardiac diseases, such as infarction and heart failure, and elevated miR-1 levels have been implicated in the development of arrhythmia in these disease settings.^19,20 Yang et al¹⁹ attributed the proarrhythmic effects of miR-1 to down regulation of connexin 43 and the inward rectifier K channel (Kir2.1), which they identified as targets for miR-1. Our study demonstrated that altered Ca²⁺ signaling presents another potential arrhythmogenic mechanism for miR-1. In vivo, electric remodeling and alterations of Ca²⁺ signaling are likely to act together to induce arrhythmia. For example, the probability of DADs reaching the threshold for action potential for any given magnitude of spontaneous Ca²⁺ release would be expected to increase on inhibition of Kir2.1 because of increased membrane resistance.³⁷

A potential limitation of our study is that the experiments were performed using primary cultures of adult rat ventricular myocytes, which are known to change their characteristics over time in culture. We did not find a significant difference in I_Ca density either with or without ISO between cells cultured for 48 hours and freshly isolated myocytes (supplemental Figure VIII). Therefore, the EC coupling machinery of rat cardiomyocytes during the first 48 hours seems to be largely preserved. This result is consistent with the recent study by Banyasz et al,³⁸ which showed that the most profound changes in morphometric and functional parameters, including T-tubular density and I_Ca, in rat ventricular myocytes take place after three days in culture.

In conclusion, our results identified a new important target for miR-1 in the heart, namely the PP2A regulatory subunit B56. Through translational inhibition of this mRNA target, miR-1 causes CaMKII-dependent hyperphosphorylation of RyR2, enhances RyR2 activity, and promotes arrhythmogenic SR Ca²⁺ release. This mechanism could contribute to induction of arrhythmia in disease states accompanied by elevated miR-1.

	Acknowledgments

 
Sources of Funding

This study was supported by American Heart Association Scientist Development Grants (to D.T. and A.E.B.) and NIH grants (to S.G., T.S.E., and D.S.F.).

Disclosures

None.

	Footnotes

 
Original received June 16, 2008; revision received December 8, 2008; accepted December 30, 2008.

	References

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