Published online before print June 12, 2008, doi: 10.1161/CIRCRESAHA.108.178590
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Submitted on April 29, 2008
Revised on May 29, 2008
Accepted on May 30, 2008
Engineering Robust and Functional Vascular Networks In Vivo With Human Adult and Cord Blood–Derived Progenitor Cells
Juan M. Melero-Martin ;
From the Vascular Biology Program and Department of Surgery, Children's Hospital Boston (J.M.M.-M., M.E.D.O., S.-Y.K., Z.A.K., J.B.), and Division of Cardiology, Beth Israel Deaconess Medical Center (L.Y., P.O.), Harvard Medical School, Boston Mass.
* To whom correspondence should be addressed. E-mail: joyce.bischoff{at}childrens.harvard.edu.
The success of therapeutic vascularization and tissue engineering will rely on our ability to create vascular networks using human cells that can be obtained readily, can be expanded safely ex vivo, and can produce robust vasculogenic activity in vivo. Here we describe the formation of functional microvascular beds in immunodeficient mice by coimplantation of human endothelial and mesenchymal progenitor cells isolated from blood and bone marrow. Evaluation of implants after 1 week revealed an extensive network of human blood vessels containing erythrocytes, indicating the rapid formation of functional anastomoses within the host vasculature. The implanted endothelial progenitor cells were restricted to the luminal aspect of the vessels; mesenchymal progenitor cells were adjacent to lumens, confirming their role as perivascular cells. Importantly, the engineered vascular networks remained patent at 4 weeks in vivo. This rapid formation of long-lasting microvascular networks by postnatal progenitor cells obtained from noninvasive sources constitutes an important step forward in the development of clinical strategies for tissue vascularization.
Key words: vascular networks • endothelial progenitor cells • mesenchymal stem cells • mesenchymal progenitor cells • tissue engineering • regenerative medicine • vasculogenesis • angiogenesis
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