Circulation Research. 2006;98:e20-e25
Published online before print January 26, 2006, doi: 10.1161/01.RES.0000205765.28940.93
Published online before print January 26, 2006, doi: 10.1161/01.RES.0000205765.28940.93
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© 2006 American Heart Association, Inc.
UltraRapid Communication |
CD34–/CD133+/VEGFR-2+ Endothelial Progenitor Cell Subpopulation With Potent Vasoregenerative Capacities
From the Department for Internal Medicine III (E.B.F., K.W., J.S.), University Hospital of the Saarland; and Department for Internal Medicine II (G.N., N.W.), University Hospital Bonn, Germany.
Correspondence to Prof Dr Georg Nickenig, Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany. E-mail georg.nickenig{at}ukb.uni-bonn.de
Our goal was to identify functionally important subpopulations within the heterogenous group of endothelial progenitor cells (EPC). Fluorescence-activated cell sorter analysis of CD133+ progenitor cells revealed the presence of CD34+ and CD34– subpopulations. CD34–/133+ progenitors differentiate into CD34+/133+ EPC, adhere more potently than these in response to SDF-1, and rapidly home to sites of limb ischemia in human volunteers. In human coronary atherectomy samples, fewer CD34–/133+ than CD34+/133+ EPC are present in stable plaques, whereas cell numbers increase with a reversion of the ratio in unstable lesions. In CD34–/133+ EPC-injected nude mice, more transplanted cells coexpressing endothelial markers home to carotid artery lesion endothelium than in CD34+/133+-injected mice. In the former, lesions were smaller and reendothelialization higher than in the latter. We identified a new CD34–/133+ EPC subpopulation, which is apparently a precursor of "classical" CD34+/133+ EPC, and functionally more potent than these with respect to homing and vascular repair.
Key Words: endothelial progenitor cells • CD34/CD133 • arterial injury • reendothelialization • atherosclerosis
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