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(Circulation Research. 2005;96:999.)
© 2005 American Heart Association, Inc.

Cellular Biology

Interdependency of ß-Adrenergic Receptors and CFTR in Regulation of Alveolar Active Na⁺ Transport

Gökhan M. Mutlu, Yochai Adir, Mohammed Jameel, Alexander T. Akhmedov, Lynn Welch, Vidas Dumasius, Fan Jing Meng, Joseph Zabner, Craig Koenig, Erin Rachel Lewis, Rajesh Balagani, Geri Traver, Jacob I. Sznajder, Phillip Factor

From the Pulmonary and Critical Care Medicine (G.M.M., Y.A., L.W., J.I.S.), Northwestern University Feinberg School of Medicine, Chicago, Ill; Evanston Northwestern Healthcare Research Institute (M.J., C.K., R.B.), Evanston Ill; Pulmonary, Allergy, and Critical Care Medicine (A.T.A., F.J.M., E.R.L., P.F.), Columbia University Medical Center, New York, NY; University of Illinois College of Medicine (V.D.), Chicago, Ill; and the University of Iowa College of Medicine (J.Z.,G.T.), Iowa City, Iowa.

Correspondence to Phillip Factor, DO, Pulmonary, Allergy, and Critical Care Medicine, Columbia University Medical Center, P&S 8-425, 630 W. 168th St, New York, NY 10032. E-mail phf2103{at}columbia.edu

ß-Adrenergic receptors (ßAR) regulate active Na⁺ transport in the alveolar epithelium and accelerate clearance of excess airspace fluid. Accumulating data indicates that the cystic fibrosis transmembrane conductance regulator (CFTR) is important for upregulation of the active ion transport that is needed to maintain alveolar fluid homeostasis during pulmonary edema. We hypothesized that ßAR regulation of alveolar active transport may be mediated via a CFTR dependent pathway. To test this hypothesis we used a recombinant adenovirus that expresses a human CFTR cDNA (adCFTR) to increase CFTR function in the alveolar epithelium of normal rats and mice. Alveolar fluid clearance (AFC), an index of alveolar active Na⁺ transport, was 92% greater in CFTR overexpressing lungs than controls. Addition of the Cl^– channel blockers NPPB, glibenclamide, or bumetanide and experiments using Cl^– free alveolar instillate solutions indicate that the accelerated AFC in this model is due to increased Cl^– channel function. Conversely, CFTR overexpression in mice with no ß₁- or ß₂-adrenergic receptors had no effect on AFC. Overexpression of a human ß₂AR in the alveolar epithelium significantly increased AFC in normal mice but had no effect in mice with a non-functional human CFTR gene (508 mutation). These studies indicate that upregulation of alveolar CFTR function speeds clearance of excess fluid from the airspace and that CFTRs effect on active Na⁺ transport requires the ßAR. These studies reveal a previously undetected interdependency between CFTR and ßAR that is essential for upregulation of active Na⁺ transport and fluid clearance in the alveolus.

Key Words: pulmonary edema • cystic fibrosis transmembrane conductance regulator • alveolar fluid clearance • chloride channel • ß-adrenergic receptors

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