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Urokinase-Type Plasminogen Activator Receptor Is Involved in Mediating the Apoptotic Effect of Cleaved High Molecular Weight Kininogen in Human Endothelial Cells

From the Sol Sherry Thrombosis Research Center (D.J.C., Y.-L.G., R.W.C.), Department of Medicine (R.W.C.), Temple University School of Medicine, Philadelphia, Pa.

Correspondence to Robert W. Colman, MD, Temple University School of Medicine, 3400 N Broad St, Philadelphia, PA 19140. E-mail colmanr{at}temple.edu

Cleaved high molecular weight kininogen (HKa) has been shown to inhibit in vivo neovascularization and induce apoptosis of endothelial cells. We have shown that HKa-induced apoptosis correlated with its antiadhesive effect and was regulated by extracellular matrix (ECM) proteins. In this study, we identified the urokinase-type plasminogen activator receptor (uPAR) as a target of HKa activity at the endothelial cell surface. Anti-uPAR antibodies blocked the apoptotic effect of HKa. Further studies revealed that uPAR formed a signaling complex containing integrin _vß₃ or ₅ß₁, caveolin, and Src kinase Yes in endothelial cells. HKa physically disrupted the formation of this complex in a manner that paralleled its apoptotic effect. For the first time, our results provide a mechanistic explanation for the previous observation that HKa selectively induces apoptosis of endothelial cells grown on vitronectin, but not cells grown on fibronectin. These data also resolve the controversial role of uPAR in mediating the apoptotic and antiadhesive activities of HKa.

Key Words: urokinase-type plasminogen activator receptor • high molecular weight kininogen • endothelial cells • apoptosis • angiogenesis

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