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(Circulation Research. 2004;94:866.)
© 2004 American Heart Association, Inc.

Reviews

Use of Chimeric Fluorescent Proteins and Fluorescence Resonance Energy Transfer to Monitor Cellular Responses

Manuela Zaccolo

From the Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Padova, Italy.

Correspondence to Manuela Zaccolo, Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy. E-mail manuela.zaccolo{at}unipd.it

This Review is part of a thematic series on Imaging of Cardiovascular Cells and Tissues, which includes the following articles:

Use of Chimeric Fluorescent Proteins and Fluorescence Resonance Energy Transfer to Monitor Cellular Responses
Imaging Microdomain Ca²⁺ in Muscle
CellsOptical Imaging of the HeartExamining Intracellular
Organelle Function Using Fluorescent ProbesTwo-Photon Microscopy of
Cells and Tissue

Brian O’Rourke Guest Editor

In recent years, the development of new technologies based on the green fluorescent protein and fluorescence resonance energy transfer has introduced a new perspective in the study of cell biology. Real-time imaging of fluorescent biosensors has made it possible to directly visualize individual molecular events as they happen in intact, live cells, providing important and original insights for our understanding of biologically relevant problems. This review discusses some essential methodological aspects concerning the generation and use of fluorescence resonance energy transfer-based biosensors and presents selected examples of specific applications that highlight the power of this technology.

Key Words: green fluorescent protein • fluorescence resonance energy transfer • cell signaling • microscopy • imaging

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