Published online before print December 18, 2003, doi: 10.1161/01.RES.0000113781.08139.81
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© 2004 American Heart Association, Inc.
Molecular Medicine |
PPAR
Activators Inhibit Vascular Endothelial Growth Factor Receptor-2 Expression by Repressing Sp1-Dependent DNA Binding and Transactivation
From the Department of Dermatology (M.M., M.S., K.R., R.K., J.G.), Molecular Cardiology, Department of Internal Medicine IV (C.U.), Klinikum der J.W. Goethe-Universität, Frankfurt am Main, Germany; Institut für Molekularbiologie und Tumorforschung (G.S.), Philipps-Universität, Marburg, Germany; Département d’Athérosclérose (B.S.), UR545 INSERM, Institut Pasteur de Lille, Lille, France; and the Department of Molecular Biology (J.G.), Max-Planck-Institut für Physiologische und Klinische Forschung, Bad Nauheim, Germany.
Correspondence to Jens Gille, MD, Zentrum der Dermatologie, Klinikum der J.W. Goethe-Universität, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany. E-mail Gille{at}em.uni-frankfurt.de
Peroxisome proliferator–activated receptors (PPARs) are ligand-activated transcription factors, originally implicated in the regulation of lipid and glucose homeostasis. In addition, natural and synthetic PPAR activators may control inflammatory processes by inhibition of distinct proinflammatory genes. As signaling via the vascular endothelial growth factor receptor-2 (VEGFR2) pathway is critical for angiogenic responses during chronic inflammation, we explored whether known antiinflammatory effects of PPAR ligands are mediated in part through diminished VEGFR2 expression. In this study, PPAR
agonists are found to inhibit endothelial VEGFR2 expression, whereas predominant PPAR
ligands remained without discernible effects. Time- and concentration-dependent inhibition is demonstrated both at the level of protein and mRNA VEGFR2 expression. Inhibitory effects of PPAR agonists on transcriptional activity of the VEGFR2 promoter are conveyed by an element located between base pairs -60 and -37 that contains two adjacent consensus Sp1 transcription factor binding sites. Constitutive Sp1-containing complex formation to this sequence is decreased by PPAR treatment, indicating that VEGFR2 gene expression is inhibited by repressing Sp1 site-dependent DNA binding and transactivation. Our coimmunoprecipitation experiments revealed enhanced protein interactions between PPAR and Sp1 on PPAR activation, thus constituting a probable mechanism by which PPAR activators decrease Sp-dependent binding activity to the VEGFR2 promoter. Hence, molecular mechanisms by which PPARs modulate the rate of gene transcription may include direct interactions between specific transcription factors and PPARs that ultimately result in reduced DNA binding to their respective response elements.
Key Words: endothelial growth factor receptors • peroxisome proliferator–activated receptors • inflammation • transcription factors • promoter regions
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