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(Circulation Research. 2003;93:595.)
© 2003 American Heart Association, Inc.

Review

Identification of Novel Signaling Complexes by Functional Proteomics

Peipei Ping

From the Department of Physiology Medicine and the Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, Calif.

Correspondence to Peipei Ping, PhD, Professor of Physiology and Medicine, Cardiovascular Laboratory, UCLA School of Medicine, MRL Building, Suite 1609, 675 Charles Young Dr, Los Angeles, CA 90095. E-mail peipeiping{at}hotmail.com or peipeiping@earthlink.net

Jennifer E. Van Eyk Guest Editor This Review is part of a thematic series on Proteomics, which includes the following articles:

Cardiovascular Proteomics: Evolution and Potential

Applied Proteomics: Mitochondrial Proteins and Effect on Function

Organelle Proteomics: Implications for Subcellular Fractionation in Proteomics

Identification of Novel Signaling Complexes by Functional Proteomics

Proteomic Approaches to Analyze the Dynamic Relationships Between Nucleocytoplasmic Protein Glycosylation and Phosphorylation

Proteomics in the Cardiomyopathies and Heart Failure: A Step Beyond Genomics

Glycosylation of Apolipoprotein E

The rapid development of proteomic technologies, combined with the completion of the Human Genome Map, has enabled the compiling of an unprecedented inventory of cellular proteins. Functional proteomics is an emerging field that aims to utilize the enormous amount of information provided by these proteomic technologies to understand the functions of cellular proteins. The utility of functional proteomics has been recently exploited to elucidate cellular mechanisms in numerous fields, of particular salience in the area of signal transduction. This review presents a functional proteomic approach for the study of cardiac cell signaling. It illustrates the strategies by which the subproteome of a targeted signaling system is characterized in an unbiased fashion, the manner in which the biochemical functions of this subproteome are assessed using established molecular and protein chemistry methods, and the challenges associated with these studies.

Key Words: functional subproteomes • multiprotein complexes • signal transduction • ischemic injury • cardiac cell death

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