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(Circulation Research. 2003;93:1026.)
© 2003 American Heart Association, Inc.

Reports

Role of 14-3-3-Mediated p38 Mitogen-Activated Protein Kinase Inhibition in Cardiac Myocyte Survival

Shaosong Zhang, Jie Ren, Cindy E. Zhang, Ilya Treskov, Yibin Wang, Anthony J. Muslin

From the Center for Cardiovascular Research, Departments of Medicine (S.Z., J.R., C.E.Z., I.T., A.J.M.) and Cell Biology and Physiology (S.Z., J.R., C.E.Z., A.J.M.), Washington University School of Medicine, St Louis, Mo; Department of Anesthesiology and Medicine (Y.W.), David Geffen School of Medicine, UCLA, Los Angeles, Calif.

Correspondence to Anthony J. Muslin, Center for Cardiovascular Research, Box 8086, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110. E-mail amuslin{at}im.wustl.edu

Abstract

14-3-3 family members are dimeric phosphoserine-binding proteins that regulate signal transduction, apoptotic, and checkpoint control pathways. Targeted expression of dominant-negative 14-3-3 (DN-14-3-3) to murine postnatal cardiac tissue potentiates Ask1, c-jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) activation. DN-14-3-3 mice are unable to compensate for pressure overload, which results in increased mortality, dilated cardiomyopathy, and cardiac myocyte apoptosis. To evaluate the relative role of p38 MAPK activity in the DN-14-3-3 phenotype, we inhibited cardiac p38 MAPK activity by pharmacological and genetic methods. Intraperitoneal injection of SB202190, an inhibitor of p38 and p38ß MAPK activity, markedly increased the ability of DN-14-3-3 mice to compensate for pressure overload, with decreased mortality. DN-14-3-3 mice were bred with transgenic mice in which dominant-negative p38 (DN-p38) or dominant-negative p38ß (DN-p38ß) MAPK expression was targeted to the heart. Compound transgenic DN-14-3-3/DN-p38ß mice, and to a lesser extent compound transgenic DN-14-3-3/DN-p38 mice, exhibited reduced mortality and cardiac myocyte apoptosis in response to pressure overload, demonstrating that DN-14-3-3 promotes cardiac apoptosis due to stimulation of p38 MAPK activity.

Key Words: signal transduction • apoptosis • cardiac • 14-3-3 protein • p38 mitogen-activated protein kinase

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