doi: 10.1161/01.RES.0000071748.48338.25
© 2003 American Heart Association, Inc.
Reviews |
Organelle Proteomics
Implications for Subcellular Fractionation in Proteomics
From the Department of Histology and Molecular Cell Biology, Institute of Anatomy and Histology, University of Innsbruck, Austria.
Correspondence to Lukas A. Huber, Institute of Anatomy and Histology, Department of Histology and Molecular Cell Biology, University of Innsbruck, 6020 Innsbruck, Austria. E-mail Lukas.A.Huber{at}uibk.ac.at
Jennifer E. Van Eyk Guest Editor This Review is part of a thematic series on Proteomics, which includes the following articles:Cardiovascular Proteomics: Evolution and PotentialApplied Proteomics: Mitochondrial Proteins and Effect on FunctionOrganelle Proteomics: Implications for Subcellular Fractionation in ProteomicsProteomics in the Cardiomyopathies and Heart Failure: A Step Beyond GenomicsGlycosylation of Apolipoprotein EPosttranslational ModificationsIdentification of Novel Signaling Complexes, Modules, and Binding Partners in Cardioprotection: A FunctionalProteomic Approach
Functional proteome analysis is not restricted to the sequence information but includes the broad spectrum of structural modifications and quantitative changes of proteins to which they are subjected in different tissues and cell organelles and during the development of an organism. Cell biology has provided the means required for the analysis of the composition and properties of purified cellular elements. Subcellular fractionation is an approach universal across all cell types and tissues, including cardiac and vascular system. Subcellular fractionation and proteomics form an ideal partnership when it comes to enrichment and analysis of intracellular organelles and low abundant multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach resulting in reduced sample complexity and is most efficiently combined with high-resolution 2D gel/mass spectrometry analysis as well as with gel-independent techniques. In this study we introduce state of the art subcellular fractionation techniques and discuss their suitability, advantages, and limitations for proteomics research.
Key Words: proteomics • subcellular fractionation • organelle
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