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(Circulation Research. 2003;92:1352.)
© 2003 American Heart Association, Inc.

Integrative Physiology

Acute Inhibition of Myoglobin Impairs Contractility and Energy State of iNOS-Overexpressing Hearts

Carsten Wunderlich, Ulrich Flögel, Axel Gödecke, Jacqueline Heger, Jürgen Schrader

From the Institute for Cardiovascular Physiology, Heinrich-Heine-University, Düsseldorf, Germany.

Correspondence to Jürgen Schrader, MD, Institut für Herz-und Kreislaufphysiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, 40225 Düsseldorf, Germany. E-mail schrader{at}uni-duesseldorf.de

Elevated cardiac levels of nitric oxide (NO) generated by inducible nitric oxide synthase (iNOS) have been implicated in the development of heart failure. The surprisingly benign phenotype of recently generated mice with cardiac-specific iNOS overexpression (TGiNOS) provided the rationale to investigate whether NO scavenging by oxymyoglobin (MbO₂) yielding nitrate and metmyoglobin (metMb) is involved in preservation of myocardial function in TGiNOS mice. ¹H nuclear magnetic resonance (NMR) spectroscopy was used to monitor changes of cardiac myoglobin (Mb) metabolism in isolated hearts of wild-type (WT) and TGiNOS mice. NO formation by iNOS resulted in a significant decrease of the MbO₂ signal and a concomitantly emerging metMb signal in spectra of TGiNOS hearts only (MbO₂: -46.3±38.4 µmol/kg, metMb: +41.4±17.6 µmol/kg, n=6; P<0.05) leaving contractility and energetics unaffected. Inhibition of the Mb-mediated NO degradation by carbon monoxide (20%) led to a deterioration of myocardial contractility in TGiNOS hearts (left ventricular developed pressure: 78.2±8.2% versus 96.7±4.6% of baseline, n=6; P<0.005), which was associated with a profound pertubation of cardiac energy state as assessed by ³¹P NMR spectroscopy (eg, phosphocreatine: 13.3±1.3 mmol/L (TGiNOS) versus 15.9±0.7 mmol/L (WT), n=6; P<0.005). These alterations could be fully antagonized by the NOS inhibitor S-ethylisothiourea. Our findings demonstrate that myoglobin serves as an important cytoplasmic buffer of iNOS-derived NO, which determines the functional consequences of iNOS overexpression.

Key Words: inducible nitric oxide synthase • myoglobin • cardiac contractility and energetics • magnetic resonance spectroscopy

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