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(Circulation Research. 2002;91:565.)
© 2002 American Heart Association, Inc.

MiniReviews

Serial Analysis of Gene Expression

Technical Considerations and Applications to Cardiovascular Biology

Willmar D. Patino, Omar Y. Mian, Paul M. Hwang

From the Cardiovascular Branch, National Heart, Lung, and Blood Institute, The National Institutes of Health, Bethesda, Md.

Correspondence to Paul M. Hwang, the Cardiovascular Branch, National Heart, Lung, and Blood Institute, The National Institutes of Health, Building 10, Rm 7B15, 10 Center Dr, Bethesda, MD 20892. E-mail hwangp{at}nhlbi.nih.gov

Abstract

It has been 7 years since serial analysis of gene expression (SAGE) and microarray hybridization techniques were simultaneously introduced to allow the screening of thousands of expressed genes. Both techniques have stood up to the test of time as evidenced by their widespread use, and both have been used for studying cardiovascular diseases. SAGE has been used more extensively to study cancer cells, but it has also been used to examine gene expression in systems as divergent as rice seedlings, yeast, and Caenorhabditis elegans. In this review, a summary of the advances in SAGE technology and its unique attributes and potential applications to the cardiovascular system will be presented.

Key Words: expression • gene • cardiovascular

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