Circulation Research. 2002;91:70-76
Published online before print May 23, 2002, doi: 10.1161/01.RES.0000023391.40106.A8
Published online before print May 23, 2002, doi: 10.1161/01.RES.0000023391.40106.A8
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© 2002 American Heart Association, Inc.
Integrative Physiology |
Impairment of Store-Operated Ca2+ Entry in TRPC4-/- Mice Interferes With Increase in Lung Microvascular Permeability
From the Department of Pharmacology, The University of Illinois, Chicago, Ill; and Universitat des Saarlandes (M.F., V.F.), Institut fur Pharmakologie und Toxikologie, Homburg, Germany.
Correspondence to Asrar B. Malik, PhD, Distinguished Professor and Head, Dept of Pharmacology M/C868, College of Medicine, University of Illinois at Chicago, 835 S Wolcott Ave, Chicago, IL 60612. E-mail abmalik{at}uic.edu
We investigated the possibility that the TRPC gene family of putative store-operated Ca2+ entry channels contributes to the increase in microvascular endothelial permeability by prolonging the rise in intracellular Ca2+ signaling. Studies were made in wild-type (wt) and TRPC4 knockout (TRPC4-/-) mice and lung vascular endothelial cells (LECs) isolated from these animals. RT-PCR showed expression of TRPC1, TRPC3, TRPC4, and TRPC6 mRNA in wt LECs, but TRPC4 mRNA expression was not detected in TRPC4-/- LECs. We studied the response to thrombin because it is known to increase endothelial permeability by the activation of G protein-coupled proteinase-activated receptor-1 (PAR-1). In wt LECs, thrombin or PAR-1 agonist peptide (TFLLRNPNDK-NH2) resulted in a prolonged Ca2+ transient secondary to influx of Ca2+. Ca2+ influx activated by thrombin was blocked by La3+ (1 µmol/L). In TRPC4-/- LECs, thrombin or TFLLRNPNDK-NH2 produced a similar initial increase of intracellular Ca2+ secondary to Ca2+ store depletion, but Ca2+ influx induced by these agonists was drastically reduced. The defect in Ca2+ influx in TRPC4-/- endothelial cells was associated with lack of thrombin-induced actin-stress fiber formation and a reduced endothelial cell retraction response. In isolated-perfused mouse lungs, the PAR-1 agonist peptide increased microvessel filtration coefficient (Kf,c), a measure of vascular permeability, by a factor of 2.8 in wt and 1.4 in TRPC4-/-; La3+ (1 µmol/L) addition to wt lung perfusate reduced the agonist effect to that observed in TRPC4-/-. These results show that TRPC4-dependent Ca2+ entry in mouse LECs is a key determinant of increased microvascular permeability.
Key Words: mouse lung endothelial cells • thrombin-induced Ca2+ influx • TRPC4 knockout • lung microvascular permeability
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