Circulation Research. 2002;90:309-316
Published online before print January 24, 2002, doi: 10.1161/hh0302.105660
Published online before print January 24, 2002, doi: 10.1161/hh0302.105660
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2002 American Heart Association, Inc.
Cellular Biology |
Protein Kinase A Phosphorylation of the Ryanodine Receptor Does Not Affect Calcium Sparks in Mouse Ventricular Myocytes
From the Department of Physiology and Cardiovascular Institute (Y.L., G.A.M., D.M.B.), Loyola University Chicago, Stritch School of Medicine, Maywood, Ill; Department of Pharmacology and Cell Biophysics (E.G.K.), University of Cincinnati College of Medicine, Cincinnati, Ohio.
Correspondence to Donald M. Bers, PhD, Department of Physiology, Loyola University Chicago, 2160 S First Ave, Maywood, IL 60153. E-mail dbers{at}lumc.edu
Ryanodine receptor (RyR) phosphorylation by protein kinase A (PKA) may be important in modulating resting sarcoplasmic reticulum (SR) Ca2+ release, especially in heart failure. However, clear cellular data on PKA-dependent modulation of cardiac RyRs is limited because of difficulty in distinguishing between PKA effects on RyR, phospholamban (PLB), and Ca2+ current. To clarify this, we measured resting Ca2+ sparks in streptolysin-O permeabilized ventricular myocytes from wild-type (WT) and PLB knockout (PLB-KO) mice and transgenic mice expressing only double-mutant PLB (PLB-DM) that lacks the regulatory phosphorylation sites (S16A/T17A). In WT myocytes, cAMP dramatically increased Ca2+ spark frequency (CaSpF) by 2- and 3-fold when [Ca2+] was clamped at 50 and 10 nmol/L (and the SR Ca2+ content also rose by 40% and 50%). However, in PLB-KO and PLB-DM, neither CaSpF nor SR Ca2+ load was changed by the addition of 10 µmol/L cAMP (even with phosphatase inhibition). PKA activation also increased Ca2+ spark amplitude, duration, and width in WT, but not in PLB-KO or PLB-DM. RyR phosphorylation was confirmed by measurements of 32P incorporation on immunoprecipitated RyR. In intact resting myocytes, PKA activation increased CaSpF 2.8-fold in WT, but not in PLB-KO, confirming results in permeabilized myocytes. We conclude that the PKA-dependent increase in myocyte CaSpF and size is entirely attributable to PLB phosphorylation and consequent enhanced SR Ca2+ load. PKA does not seem to have any appreciable effect on resting RyR function in these ventricular myocytes. Moreover, the data provide compelling evidence that elevated intra-SR [Ca2+] increases RyR gating independent of cytosolic [Ca2+] (which was clamped).
Key Words: cardiac muscle • excitation-contraction coupling • calcium transport • sarcoplasmic reticulum
This article has been cited by other articles:
 |
|
 |
|
  X. Zhang, Y. N. Tallini, Z. Chen, L. Gan, B. Wei, R. Doran, L. Miao, H.-B. Xin, M. I. Kotlikoff, and G. Ji Dissociation of FKBP12.6 from ryanodine receptor type 2 is regulated by cyclic ADP-ribose but not {beta}-adrenergic stimulation in mouse cardiomyocytes Cardiovasc Res, November 1, 2009; 84(2): 253 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zhou, Y.-T. Zhao, Y.-B. Guo, S.-M. Xu, S.-H. Bai, E. G. Lakatta, H. Cheng, X.-M. Hao, and S.-Q. Wang {beta}-Adrenergic signaling accelerates and synchronizes cardiac ryanodine receptor response to a single L-type Ca2+ channel PNAS, October 20, 2009; 106(42): 18028 - 18033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cheng and W. J. Lederer Calcium Sparks Physiol Rev, October 1, 2008; 88(4): 1491 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. MacDonnell, G. Garcia-Rivas, J. A. Scherman, H. Kubo, X. Chen, H. Valdivia, and S. R. Houser Adrenergic Regulation of Cardiac Contractility Does Not Involve Phosphorylation of the Cardiac Ryanodine Receptor at Serine 2808 Circ. Res., April 25, 2008; 102(8): e65 - e72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ikeda, M. Hoshijima, and K. R. Chien Toward Biologically Targeted Therapy of Calcium Cycling Defects in Heart Failure Physiology, February 1, 2008; 23(1): 6 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Orchard and F. Brette t-tubules and sarcoplasmic reticulum function in cardiac ventricular myocytes Cardiovasc Res, January 15, 2008; 77(2): 237 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Liu and S. G. Priori Disruption of calcium homeostasis and arrhythmogenesis induced by mutations in the cardiac ryanodine receptor and calsequestrin Cardiovasc Res, January 15, 2008; 77(2): 293 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. George Sarcoplasmic reticulum Ca2+ leak in heart failure: mere observation or functional relevance? Cardiovasc Res, January 15, 2008; 77(2): 302 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lim, L. Venetucci, D. A. Eisner, and B. Casadei Does nitric oxide modulate cardiac ryanodine receptor function? Implications for excitation-contraction coupling Cardiovasc Res, January 15, 2008; 77(2): 256 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. James Inhibition of SR Ca2+ uptake: A novel action of the RyR2-FKBP12.6 antagonist K201 Cardiovasc Res, November 1, 2007; 76(2): 199 - 201. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Xiao, X. Tian, W. Xie, P. P. Jones, S. Cai, X. Wang, D. Jiang, H. Kong, L. Zhang, K. Chen, et al. Functional Consequence of Protein Kinase A-dependent Phosphorylation of the Cardiac Ryanodine Receptor: SENSITIZATION OF STORE OVERLOAD-INDUCED Ca2+ RELEASE J. Biol. Chem., October 12, 2007; 282(41): 30256 - 30264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lehnart and A. R. Marks Regulation of Ryanodine Receptors in the Heart Circ. Res., October 12, 2007; 101(8): 746 - 749. [Full Text] [PDF]
|
|
|
|
|
|
N. A. Benkusky, C. S. Weber, J. A. Scherman, E. F. Farrell, T. A. Hacker, M. C. John, P. A. Powers, and H. H. Valdivia Intact {beta}-Adrenergic Response and Unmodified Progression Toward Heart Failure in Mice With Genetic Ablation of a Major Protein Kinase A Phosphorylation Site in the Cardiac Ryanodine Receptor Circ. Res., October 12, 2007; 101(8): 819 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Seidler, G. Hasenfuss, and L. S. Maier Targeting Altered Calcium Physiology in the Heart: Translational Approaches to Excitation, Contraction, and Transcription Physiology, October 1, 2007; 22(5): 328 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen-Izu, C. W. Ward, W. Stark Jr., T. Banyasz, M. P. Sumandea, C. W. Balke, L. T. Izu, and X. H. T. Wehrens Phosphorylation of RyR2 and shortening of RyR2 cluster spacing in spontaneously hypertensive rat with heart failure Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2409 - H2417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Meng, B. Xiao, S. Cai, X. Huang, F. Li, J. Bolstad, R. Trujillo, J. Airey, S. R. W. Chen, T. Wagenknecht, et al. Three-Dimensional Localization of Serine 2808, a Phosphorylation Site in Cardiac Ryanodine Receptor J. Biol. Chem., August 31, 2007; 282(35): 25929 - 25939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Copello, A. V. Zima, P. L. Diaz-Sylvester, M. Fill, and L. A. Blatter Ca2+ entry-independent effects of L-type Ca2+ channel modulators on Ca2+ sparks in ventricular myocytes Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2129 - C2140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Katra, T. Oya, G. S. Hoeker, and K. R. Laurita Ryanodine receptor dysfunction and triggered activity in the heart Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2144 - H2151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nattel, A. Maguy, S. Le Bouter, and Y.-H. Yeh Arrhythmogenic Ion-Channel Remodeling in the Heart: Heart Failure, Myocardial Infarction, and Atrial Fibrillation Physiol Rev, April 1, 2007; 87(2): 425 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zissimopoulos, N. Docrat, and F. A. Lai Redox Sensitivity of the Ryanodine Receptor Interaction with FK506-binding Protein J. Biol. Chem., March 9, 2007; 282(10): 6976 - 6983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yamaguchi and G. Meissner Does Ca2+/Calmodulin-Dependent Protein Kinase {delta}c Activate or Inhibit the Cardiac Ryanodine Receptor Ion Channel? Circ. Res., February 16, 2007; 100(3): 293 - 295. [Full Text] [PDF]
|
|
|
|
|
|
K. R. Sipido CaM or cAMP: Linking {beta}-Adrenergic Stimulation to 'Leaky' RyRs Circ. Res., February 16, 2007; 100(3): 296 - 298. [Full Text] [PDF]
|
|
|
|
|
|
J. Curran, M. J. Hinton, E. Rios, D. M. Bers, and T. R. Shannon {beta}-Adrenergic Enhancement of Sarcoplasmic Reticulum Calcium Leak in Cardiac Myocytes Is Mediated by Calcium/Calmodulin-Dependent Protein Kinase Circ. Res., February 16, 2007; 100(3): 391 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. Collis, M. B. Meyers, J. Zhang, C. K. L. Phoon, E. A. Sobie, W. A. Coetzee, and G. I. Fishman Expression of a sorcin missense mutation in the heart modulates excitation-contraction coupling FASEB J, February 1, 2007; 21(2): 475 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Venetucci, A. W. Trafford, and D. A. Eisner Increasing Ryanodine Receptor Open Probability Alone Does Not Produce Arrhythmogenic Calcium Waves: Threshold Sarcoplasmic Reticulum Calcium Content Is Required Circ. Res., January 5, 2007; 100(1): 105 - 111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Bers Altered Cardiac Myocyte Ca Regulation In Heart Failure. Physiology, December 1, 2006; 21(6): 380 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Bers The Beat Goes On: Diastolic Noise That Just Won't Quit Circ. Res., October 27, 2006; 99(9): 921 - 923. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Altamirano, Y. Li, J. DeSantiago, V. Piacentino 3rd, S. R. Houser, and D. M. Bers The inotropic effect of cardioactive glycosides in ventricular myocytes requires Na+-Ca2+ exchanger function J. Physiol., September 15, 2006; 575(3): 845 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kockskamper and B. Pieske Phosphorylation of the Cardiac Ryanodine Receptor by Ca2+/Calmodulin-Dependent Protein Kinase II: The Dominating Twin of Protein Kinase A? Circ. Res., August 18, 2006; 99(4): 333 - 335. [Full Text] [PDF]
|
|
|
|
|
|
T. Guo, T. Zhang, R. Mestril, and D. M. Bers Ca2+/Calmodulin-Dependent Protein Kinase II Phosphorylation of Ryanodine Receptor Does Affect Calcium Sparks in Mouse Ventricular Myocytes Circ. Res., August 18, 2006; 99(4): 398 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Hagen, O. Bayguinov, and K. M. Sanders VIP and PACAP regulate localized Ca2+ transients via cAMP-dependent mechanism Am J Physiol Cell Physiol, August 1, 2006; 291(2): C375 - C385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Carter, J. Colyer, and R. Sitsapesan Maximum Phosphorylation of the Cardiac Ryanodine Receptor at Serine-2809 by Protein Kinase A Produces Unique Modifications to Channel Gating and Conductance Not Observed at Lower Levels of Phosphorylation Circ. Res., June 23, 2006; 98(12): 1506 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Lehnart, C. Terrenoire, S. Reiken, X. H. T. Wehrens, L.-S. Song, E. J. Tillman, S. Mancarella, J. Coromilas, W. J. Lederer, R. S. Kass, et al. Stabilization of cardiac ryanodine receptor prevents intracellular calcium leak and arrhythmias PNAS, May 16, 2006; 103(20): 7906 - 7910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Valverde, C. Mundina-Weilenmann, M. Reyes, E. G. Kranias, A. L. Escobar, and A. Mattiazzi Phospholamban phosphorylation sites enhance the recovery of intracellular Ca2+ after perfusion arrest in isolated, perfused mouse heart Cardiovasc Res, May 1, 2006; 70(2): 335 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Litwin "Ryanogate": Who Leaked the Calcium? Circ. Res., February 3, 2006; 98(2): 165 - 168. [Full Text] [PDF]
|
|
|
|
|
|
X. H. T. Wehrens, S. E. Lehnart, S. Reiken, J. A. Vest, A. Wronska, and A. R. Marks Inaugural Article: Ryanodine receptor/calcium release channel PKA phosphorylation: A critical mediator of heart failure progression PNAS, January 17, 2006; 103(3): 511 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Obayashi, B. Xiao, B. D. Stuyvers, A. W. Davidoff, J. Mei, S.R. W. Chen, and H. E.D.J. ter Keurs Spontaneous diastolic contractions and phosphorylation of the cardiac ryanodine receptor at serine-2808 in congestive heart failure in rat Cardiovasc Res, January 1, 2006; 69(1): 140 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lawo, M. Borggrefe, C. Butter, G. Hindricks, H. Schmidinger, Y. Mika, D. Burkhoff, C. Pappone, and H. N. Sabbah Electrical Signals Applied During the Absolute Refractory Period: An Investigational Treatment for Advanced Heart Failure in Patients With Normal QRS Duration J. Am. Coll. Cardiol., December 20, 2005; 46(12): 2229 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Anderson The Fire From Within: The Biggest Ca2+ Channel Erupts and Dribbles Circ. Res., December 9, 2005; 97(12): 1213 - 1215. [Full Text] [PDF]
|
|
|
|
|
|
X. Ai, J. W. Curran, T. R. Shannon, D. M. Bers, and S. M. Pogwizd Ca2+/Calmodulin-Dependent Protein Kinase Modulates Cardiac Ryanodine Receptor Phosphorylation and Sarcoplasmic Reticulum Ca2+ Leak in Heart Failure Circ. Res., December 9, 2005; 97(12): 1314 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Sipido and D. Eisner Something old, something new: Changing views on the cellular mechanisms of heart failure Cardiovasc Res, November 1, 2005; 68(2): 167 - 174. [Full Text] [PDF]
|
|
|
|
 |
|
 I. Zahradnik, S. Gyorke, and A. Zahradnikova Calcium Activation of Ryanodine Receptor Channels--Reconciling RyR Gating Models with Tetrameric Channel Structure J. Gen. Physiol., October 31, 2005; 126(5): 515 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kontula, P. J. Laitinen, A. Lehtonen, L. Toivonen, M. Viitasalo, and H. Swan Catecholaminergic polymorphic ventricular tachycardia: Recent mechanistic insights Cardiovasc Res, August 15, 2005; 67(3): 379 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oda, M. Yano, T. Yamamoto, T. Tokuhisa, S. Okuda, M. Doi, T. Ohkusa, Y. Ikeda, S. Kobayashi, N. Ikemoto, et al. Defective Regulation of Interdomain Interactions Within the Ryanodine Receptor Plays a Key Role in the Pathogenesis of Heart Failure Circulation, June 28, 2005; 111(25): 3400 - 3410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Lindegger and E. Niggli Paradoxical SR Ca2+ release in guinea-pig cardiac myocytes after {beta}-adrenergic stimulation revealed by two-photon photolysis of caged Ca2+ J. Physiol., June 15, 2005; 565(3): 801 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Burkhoff and S. A. Ben-Haim Nonexcitatory electrical signals for enhancing ventricular contractility: rationale and initial investigations of an experimental treatment for heart failure Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2550 - H2556. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xu and T. L. Krukoff Adrenomedullin Stimulates Nitric Oxide Release from SK-N-SH Human Neuroblastoma Cells by Modulating Intracellular Calcium Mobilization Endocrinology, May 1, 2005; 146(5): 2295 - 2305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Xiao, M. T. Jiang, M. Zhao, D. Yang, C. Sutherland, F. A. Lai, M. P. Walsh, D. C. Warltier, H. Cheng, and S. R. W. Chen Characterization of a Novel PKA Phosphorylation Site, Serine-2030, Reveals No PKA Hyperphosphorylation of the Cardiac Ryanodine Receptor in Canine Heart Failure Circ. Res., April 29, 2005; 96(8): 847 - 855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mundina-Weilenmann, P. Ferrero, M. Said, L. Vittone, E. G. Kranias, and A. Mattiazzi Role of phosphorylation of Thr17 residue of phospholamban in mechanical recovery during hypercapnic acidosis Cardiovasc Res, April 1, 2005; 66(1): 114 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, W. Zhu, S. Wang, D. Yang, M. T. Crow, R.-P. Xiao, and H. Cheng Sustained {beta}1-Adrenergic Stimulation Modulates Cardiac Contractility by Ca2+/Calmodulin Kinase Signaling Pathway Circ. Res., October 15, 2004; 95(8): 798 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Kass, J. G.F. Bronzwaer, and W. J. Paulus What Mechanisms Underlie Diastolic Dysfunction in Heart Failure? Circ. Res., June 25, 2004; 94(12): 1533 - 1542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Ginsburg and D. M. Bers Modulation of excitation-contraction coupling by isoproterenol in cardiomyocytes with controlled SR Ca2+ load and Ca2+ current trigger J. Physiol., April 15, 2004; 556(2): 463 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. H. T. Wehrens, S. E. Lehnart, S. R. Reiken, S.-X. Deng, J. A. Vest, D. Cervantes, J. Coromilas, D. W. Landry, and A. R. Marks Protection from Cardiac Arrhythmia Through Ryanodine Receptor-Stabilizing Protein Calstabin2 Science, April 9, 2004; 304(5668): 292 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. H.T. Wehrens, S. E. Lehnart, S. R. Reiken, and A. R. Marks Ca2+/Calmodulin-Dependent Protein Kinase II Phosphorylation Regulates the Cardiac Ryanodine Receptor Circ. Res., April 2, 2004; 94(6): e61 - e70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Xiao, C. Sutherland, M. P. Walsh, and S.R. W. Chen Protein Kinase A Phosphorylation at Serine-2808 of the Cardiac Ca2+-Release Channel (Ryanodine Receptor) Does Not Dissociate 12.6-kDa FK506-Binding Protein (FKBP12.6) Circ. Res., March 5, 2004; 94(4): 487 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stange, L. Xu, D. Balshaw, N. Yamaguchi, and G. Meissner Characterization of Recombinant Skeletal Muscle (Ser-2843) and Cardiac Muscle (Ser-2809) Ryanodine Receptor Phosphorylation Mutants J. Biol. Chem., December 19, 2003; 278(51): 51693 - 51702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Lohse, S. Engelhardt, and T. Eschenhagen What Is the Role of {beta}-Adrenergic Signaling in Heart Failure? Circ. Res., November 14, 2003; 93(10): 896 - 906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.S Swan, K Dibb, N Negretti, S.C O'Neill, and R Sitsapesan Effects of eicosapentaenoic acid on cardiac SR Ca2+-release and ryanodine receptor function Cardiovasc Res, November 1, 2003; 60(2): 337 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rodriguez, M. S. Bhogal, and J. Colyer Stoichiometric Phosphorylation of Cardiac Ryanodine Receptor on Serine 2809 by Calmodulin-dependent Kinase II and Protein Kinase A J. Biol. Chem., October 3, 2003; 278(40): 38593 - 38600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Shannon, S. M. Pogwizd, and D. M. Bers Elevated Sarcoplasmic Reticulum Ca2+ Leak in Intact Ventricular Myocytes From Rabbits in Heart Failure Circ. Res., October 3, 2003; 93(7): 592 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Terentyev, S. Viatchenko-Karpinski, I. Gyorke, R. Terentyeva, and S. Gyorke Protein Phosphatases Decrease Sarcoplasmic Reticulum Calcium Content by Stimulating Calcium Release in Cardiac Myocytes J. Physiol., October 1, 2003; 552(1): 109 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Bers, D. A. Eisner, and H. H. Valdivia Sarcoplasmic Reticulum Ca2+ and Heart Failure: Roles of Diastolic Leak and Ca2+ Transport Circ. Res., September 19, 2003; 93(6): 487 - 490. [Full Text] [PDF]
|
|
|
|
|
|
C. H. George, G. V. Higgs, and F. A. Lai Ryanodine Receptor Mutations Associated With Stress-Induced Ventricular Tachycardia Mediate Increased Calcium Release in Stimulated Cardiomyocytes Circ. Res., September 19, 2003; 93(6): 531 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. F. Farrell, A. Antaramian, A. Rueda, A. M. Gomez, and H. H. Valdivia Sorcin Inhibits Calcium Release and Modulates Excitation-Contraction Coupling in the Heart J. Biol. Chem., September 5, 2003; 278(36): 34660 - 34666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. George, G. V. Higgs, J. J. Mackrill, and F. A. Lai Dysregulated Ryanodine Receptors Mediate Cellular Toxicity: RESTORATION OF NORMAL PHENOTYPE BY FKBP12.6 J. Biol. Chem., August 1, 2003; 278(31): 28856 - 28864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Shannon, T. Guo, and D. M. Bers Ca2+ Scraps: Local Depletions of Free [Ca2+] in Cardiac Sarcoplasmic Reticulum During Contractions Leave Substantial Ca2+ Reserve Circ. Res., July 11, 2003; 93(1): 40 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Ziolo and D. M. Bers The Real Estate of NOS Signaling: Location, Location, Location Circ. Res., June 27, 2003; 92(12): 1279 - 1281. [Full Text] [PDF]
|
|
|
|
|
|
A. G. Brittsan, K. S. Ginsburg, G. Chu, A. Yatani, B. M. Wolska, A. G. Schmidt, M. Asahi, D. H. MacLennan, D. M. Bers, and E. G. Kranias Chronic SR Ca2+-ATPase Inhibition Causes Adaptive Changes in Cellular Ca2+ Transport Circ. Res., April 18, 2003; 92(7): 769 - 776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Marks A Guide for the Perplexed: Towards an Understanding of the Molecular Basis of Heart Failure Circulation, March 25, 2003; 107(11): 1456 - 1459. [Full Text] [PDF]
|
|
|
|
|
|
W. Schillinger, J. W Fiolet, K. Schlotthauer, and G. Hasenfuss Relevance of Na+-Ca2+ exchange in heart failure Cardiovasc Res, March 15, 2003; 57(4): 921 - 933. [Full Text] [PDF]
|
|
|
|
|
|
S. R. Houser and K. B. Margulies Is Depressed Myocyte Contractility Centrally Involved in Heart Failure? Circ. Res., March 7, 2003; 92(4): 350 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Hasenfuss and T. Seidler Treatment of Heart Failure Through Stabilization of the Cardiac Ryanodine Receptor Circulation, January 28, 2003; 107(3): 378 - 380. [Full Text] [PDF]
|
|
|
|
|
|
S. Reiken, M. Gaburjakova, S. Guatimosim, A. M. Gomez, J. D'Armiento, D. Burkhoff, J. Wang, G. Vassort, W. J. Lederer, and A. R. Marks Protein Kinase A Phosphorylation of the Cardiac Calcium Release Channel (Ryanodine Receptor) in Normal and Failing Hearts. ROLE OF PHOSPHATASES AND RESPONSE TO ISOPROTERENOL J. Biol. Chem., January 3, 2003; 278(1): 444 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. del Monte and R. J Hajjar Targeting calcium cycling proteins in heart failure through gene transfer J. Physiol., January 1, 2003; 546(1): 49 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Scoote and A. J Williams The cardiac ryanodine receptor (calcium release channel): Emerging role in heart failure and arrhythmia pathogenesis Cardiovasc Res, December 1, 2002; 56(3): 359 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Jiang, A. J. Lokuta, E. F. Farrell, M. R. Wolff, R. A. Haworth, and H. H. Valdivia Abnormal Ca2+ Release, but Normal Ryanodine Receptors, in Canine and Human Heart Failure Circ. Res., November 29, 2002; 91(11): 1015 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Shannon, K. S. Ginsburg, and D. M. Bers Quantitative Assessment of the SR Ca2+ Leak-Load Relationship Circ. Res., October 4, 2002; 91(7): 594 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fill and J. A. Copello Ryanodine Receptor Calcium Release Channels Physiol Rev, October 1, 2002; 82(4): 893 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L F Santana, E G Chase, V S Votaw, M. T Nelson, and R Greven Functional coupling of calcineurin and protein kinase A in mouse ventricular myocytes J. Physiol., October 1, 2002; 544(1): 57 - 69. [Abstract] [Full Text] [PDF]
|
|