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(Circulation Research. 2001;89:723.)
© 2001 American Heart Association, Inc.

Integrative Physiology

Vascular Morphogenesis and Remodeling in a Model of Tissue Repair

Blood Vessel Formation and Growth in the Ovarian Pedicle After Ovariectomy

Sybill Patan, Lance L. Munn, Shigeru Tanda, Sylvie Roberge, Rakesh K. Jain, Rosemary C. Jones

From the Departments of Radiation Oncology (S.P., L.L.M., S.T., S.R., R.K.J.) and Anesthesia (R.C.J.), Massachusetts General Hospital and Harvard Medical School, Boston, Mass.

Correspondence to Dr S. Patan, Department of Cardiology, Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Ave, Bronx, NY 10461. E-mail spatan,aecom.yu.edu

Abstract—— To investigate mechanisms of vascular morphogenesis in tissue repair, we performed ovariectomy with resection of the corresponding branches of the ovarian vessels in nude mice. This induces a vascular network remodeling response in the healing ovarian pedicle. Reconstruction of 2000 histological serial sections demonstrated that a new vascular network composed of venous-venous loops forms in the wall of the dilated ovarian vein. Preexisting veins of all sizes, including a branch of the main artery, are subjected to segmentation. Loop formation and segmentation are based on intussusceptive microvascular growth. Loop formation is followed by elongation. Loop remodeling occurs also by intussusception and results in the formation of compound loop systems. All loop systems observed were completely patent. Blind-ending sprouts were extremely rare. Anastomoses between the preexisting vessels subjected to segmentation and the loop systems were established to include the newly formed vessels into the preexisting vascular network. The formation of an increasing number of patent loop systems likely decreases hypoxia and subsequently arrests angiogenesis with transformation of the granulation tissue into a scar. Loop formation also occurred inside a large thrombus that occluded a part of the lumen of the main vein.

Key Words: angiogenesis • endothelial cell • intussusceptive microvascular growth • tissue repair • restenosis

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