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(Circulation Research. 2001;89:111.)
© 2001 American Heart Association, Inc.

Reports

Abnormal Balance in the Angiopoietin-Tie2 System in Human Brain Arteriovenous Malformations

Tomoki Hashimoto, Tiffany Lam, Nancy J. Boudreau, Andrew W. Bollen, Michael T. Lawton, William L. Young

From the Departments of Anesthesia and Perioperative Care (T.H., T.L., W.L.Y.), Surgery (N.J.B.), Pathology (A.W.B.), Neurological Surgery (M.T.L., W.L.Y.), and Neurology (W.L.Y.), University of California, San Francisco, Calif.

Correspondence to William L. Young, MD, Center for Cerebrovascular Research, University of California, San Francisco, 1001 Potrero Ave, 3C-38, San Francisco, CA 94110. E-mail CCR{at}anesthesia.ucsf.edu

Abstract

Brain arteriovenous malformations (BAVMs) are congenital vascular lesions that often present as cerebral hemorrhage in young adults. The variable nature of the clinical course, especially with respect to spontaneous hemorrhage, recurrence, growth, and regression, suggests that BAVMs are lesions with active angiogenesis and vascular remodeling. We examined mRNA and protein expression of angiopoietin 1 (Ang1) and Ang2 by semiquantitative reverse transcriptase–polymerase chain reaction, in situ hybridization, and Western blot in BAVMs and control brains obtained from temporal lobectomy for medically intractable seizures. Although Ang1 mRNA levels were similar in BAVMs and controls, Ang1 protein levels were 30% lower in BAVMs than in controls. Ang2 mRNA levels were 40% higher and Ang2 protein levels were 8-fold higher in BAVMs than in controls. In situ hybridization showed that the Ang2 mRNA was localized to the perivascular area in BAVMs. This abnormal balance in the Ang-Tie2 system may, in part, explain the aberrant vascular phenotype in BAVMs.

Key Words: cerebral arteriovenous malformations • vascular malformations • endothelium • angiopoietin • Tie2

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