doi: 10.1161/hh0801.090193
© 2001 American Heart Association, Inc.
Reviews |
Cardiovascular Proteomics
Evolution and Potential
From the Departments of Physiology (D.K.A., I.N., J.E.V.E.) and Biochemistry (J.E.V.E.), Queen’s University, Kingston, Ontario, Canada.
Correspondence to J.E. Van Eyk, 429 Botterell Hall, Queen’s University, Kingston, Ontario, Canada K7L 3N6. E-mail JVE1{at}post.queensu.ca
Abstract—The development of proteomics is a timely one for cardiovascular research. Analyses at the organ, subcellular, and molecular levels have revealed dynamic, complex, and subtle intracellular processes associated with heart and vascular disease. The power and flexibility of proteomic analyses, which facilitate protein separation, identification, and characterization, should hasten our understanding of these processes at the protein level. Properly applied, proteomics provides researchers with cellular protein "inventories" at specific moments in time, making it ideal for documenting protein modification due to a particular disease, condition, or treatment. This is accomplished through the establishment of species- and tissue-specific protein databases, providing a foundation for subsequent proteomic studies. Evolution of proteomic techniques has permitted more thorough investigation into molecular mechanisms underlying cardiovascular disease, facilitating identification not only of modified proteins but also of the nature of their modification. Continued development should lead to functional proteomic studies, in which identification of protein modification, in conjunction with functional data from established biochemical and physiological methods, has the ability to further our understanding of the interplay between proteome change and cardiovascular disease.
Key Words: proteomics • protein modification • 2-D gel electrophoresis • mass spectrometry • cardiovascular disease • molecular mechanism
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