This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Majid, D. S. A. Articles by Navar, L. G. Search for Related Content PubMed PubMed Citation Articles by Majid, D. S. A. Articles by Navar, L. G. Related Collections Endothelium/vascular type/nitric oxide

(Circulation Research. 2001;88:347.)
© 2001 American Heart Association, Inc.

Integrative Physiology

Nitric Oxide Dependency of Arterial Pressure–Induced Changes in Renal Interstitial Hydrostatic Pressure in Dogs

Dewan S. A. Majid, Karim E. Said, Sophia A. Omoro, L. Gabriel Navar

From the Department of Physiology, Tulane University School of Medicine, New Orleans, La.

Correspondence to Dewan S.A. Majid, PhD, Department of Physiology SL39, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA 70112.

Abstract—A direct relationship between renal arterial pressure (RAP) and renal interstitial hydrostatic pressure (RIHP) has been shown under conditions of efficient renal blood flow autoregulation. Experiments were performed in six anesthetized dogs to evaluate whether these RIHP responses to changes in RAP were modified during nitric oxide (NO) inhibition with nitro-L-arginine (NLA) or after administration of NO donor agents. A microtip catheter transducer was placed underneath the renal capsule to measure RIHP. Stepwise reductions in RAP (140 to 80 mm Hg) during control conditions resulted in decreases in RIHP from its basal value of 4.7±1.1 mm Hg with a slope of 0.04±0.026 mm Hg · mm Hg^-1 along with decreases in urinary nitrate/nitrite excretion rate (U_NOxV). Renal cortical and medullary blood flows, measured by laser-Doppler flowmetry, exhibited high autoregulatory efficiency over this RAP range. The changes in RIHP during alterations in RAP were positively correlated (r=0.743; P<0.001) with the changes in U_NOxV but not with cortical or medullary blood flow. NLA infusion decreased RIHP to 1.9±0.5 mm Hg and also reduced U_NOxV from 1.8±0.2 to 0.9±0.01 nmol · min^-1 · g^-1. Infusion of NO donors restored RIHP (4.3±0.9 mm Hg) and U_NOxV (1.5±0.2 nmol · min^-1 · g^-1). During NLA infusion, the RIHP responses to reductions in RAP were markedly attenuated and were not restored even during constant-rate infusion of NO donors. The results suggest that changes in RIHP in response to alterations in RAP are associated with changes in intrarenal NO, suggesting a direct effect of NO to regulate RIHP.

Key Words: renal regional blood flow • laser-Doppler flowmetry • nitrate/nitrite excretion

This article has been cited by other articles:

				A. W. Cowley Jr Renal Medullary Oxidative Stress, Pressure-Natriuresis, and Hypertension Hypertension, November 1, 2008; 52(5): 777 - 786. [Full Text] [PDF]

				J. D. Joo, M. Kim, V. D. D'Agati, and H. T. Lee Ischemic Preconditioning Provides Both Acute and Delayed Protection against Renal Ischemia and Reperfusion Injury in Mice J. Am. Soc. Nephrol., November 1, 2006; 17(11): 3115 - 3123. [Abstract] [Full Text] [PDF]

				U. K. Dutta, J. Lane, L. J. Roberts II, and D. S. A. Majid Superoxide formation and interaction with nitric oxide modulate systemic arterial pressure and renal function in salt-depleted dogs. Experimental Biology and Medicine, March 1, 2006; 231(3): 269 - 276. [Abstract] [Full Text] [PDF]

				E. A. Dos Santos, A. J. Dahly-Vernon, K. M. Hoagland, and R. J. Roman Inhibition of the formation of EETs and 20-HETE with 1-aminobenzotriazole attenuates pressure natriuresis Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R58 - R68. [Abstract] [Full Text] [PDF]

				M. Obst, V. Gross, J. Janke, M. Wellner, W. Schneider, and F. C. Luft Pressure Natriuresis in AT2 Receptor-Deficient Mice with L-NAME Hypertension J. Am. Soc. Nephrol., February 1, 2003; 14(2): 303 - 310. [Abstract] [Full Text] [PDF]

				G. A. Eppel, G. Bergstrom, W. P. Anderson, and R. G. Evans Autoregulation of renal medullary blood flow in rabbits Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2003; 284(1): R233 - R244. [Abstract] [Full Text] [PDF]

				Y. Nishimoto, T. Tomida, H. Matsui, T. Ito, and K. Okumura Decrease in Renal Medullary Endothelial Nitric Oxide Synthase of Fructose-Fed, Salt-Sensitive Hypertensive Rats Hypertension, August 1, 2002; 40(2): 190 - 194. [Abstract] [Full Text] [PDF]

				J. L. Andersen, N. C. F. Sandgaard, and P. Bie Volume expansion during acute angiotensin II receptor (AT1) blockade and NOS inhibition in conscious dogs Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1140 - R1148. [Abstract] [Full Text] [PDF]

				D. S.A. Majid and A. Nishiyama Nitric Oxide Blockade Enhances Renal Responses to Superoxide Dismutase Inhibition in Dogs Hypertension, February 1, 2002; 39(2): 293 - 297. [Abstract] [Full Text] [PDF]