© 2000 American Heart Association, Inc.
Molecular Medicine |
Host Gene Regulation During Coxsackievirus B3 Infection in Mice
Assessment by Microarrays
From the Cardiovascular Research Laboratory (L.A.T., C.M.C., D.Y., K.S., D.W., B.M.M.), Department of Pathology and Laboratory Medicine, University of British Columbia, St. Paul’s Hospital, Vancouver, Canada, and SCIOS, Inc (G.S., L.W.S.), Sunnyvale, Calif.
Correspondence to Bruce McManus, MD, PhD, Department of Pathology and Laboratory Medicine, University of British Columbia, St. Paul’s Hospital, 1081 Burrard St, Vancouver, BC, Canada V6Z 1Y6. E-mail mcmanus{at}interchange.ubc.ca
Abstract—Host genetic responses that characterize enteroviral myocarditis have not yet been determined. The injurious and inflammatory process in heart muscle may reflect host responses of benefit to the virus and ultimately result in congestive heart failure and dilated cardiomyopathy. On the other hand, host responses within the myocardium may secure the host against acute or protracted damage. To investigate the nature of modified gene expression in comparison with normal tissue, mRNA species were assessed in myocardium using cDNA microarray technology at days 3, 9, and 30 after infection. Of 7000 clones initially screened, 169 known genes had a level of expression significantly different at 1 or more postinfection time points as compared with baseline. The known regulated genes were sorted according to their functional groups and normalized expression patterns and, subsequently, interpreted in the context of viremic, inflammatory, and healing phases of the myocarditic process.
Key Words: coxsackieviruses • gene regulation • microarrays • myocardium
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