© 2000 American Heart Association, Inc.
Molecular Medicine |
Evidence for Ca2+ Activation and Inactivation Sites on the Luminal Side of the Cardiac Ryanodine Receptor Complex
From the Department of Cardiac Medicine, National Heart & Lung Institute, Imperial College of Science, Technology & Medicine, London, UK.
Correspondence to R. Sitsapesan, Department of Cardiac Medicine, National Heart & Lung Institute, Imperial College of Science, Technology & Medicine, Dovehouse St, London SW3 6LY, UK. E-mail r.sitsapesan{at}ic.ac.uk
Abstract—We have used tryptic digestion to determine whether Ca2+ can regulate cardiac ryanodine receptor (RyR) channel gating from within the lumen of the sarcoplasmic reticulum (SR) or whether Ca2+ must first flow through the channel and act via cytosolically located binding sites. Cardiac RyRs were incorporated into bilayers, and trypsin was applied to the luminal side of the bilayer. We found that before exposure to luminal trypsin, the open probability of RyR was increased by raising the luminal [Ca2+] from 10 µmol/L to 1 mmol/L, whereas after luminal trypsin exposure, increasing the luminal [Ca2+] reduced the open probability. The modification in the response of RyRs to luminal Ca2+ was not observed with heat-inactivated trypsin, indicating that digestion of luminal sites on the RyR channel complex was responsible. Our results provide strong evidence for the presence of luminally located Ca2+ activation and inhibition sites and indicate that trypsin digestion leads to selective damage to luminal Ca2+ activation sites without affecting luminal Ca2+ inactivation sites. We suggest that changes in luminal [Ca2+] will be able to regulate RyR channel gating from within the SR lumen, therefore providing a second Ca2+-regulatory effect on RyR channel gating in addition to that of cytosolic Ca2+. This luminal Ca2+-regulatory mechanism is likely to be an important contributing factor in the potentiation of SR Ca2+ release that is observed in cardiac cells in response to increases in intra-SR [Ca2+].
Key Words: ryanodine receptors • Ca2+ release • cardiac excitation-contraction coupling • sarcoplasmic reticulum
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