© 2000 American Heart Association, Inc.
Integrative Physiology |
Direct Evidence for Increased Hydroxyl Radicals Originating From Superoxide in the Failing Myocardium
From Cardiovascular Medicine (T.I., H.T., S.K., N.S., S.H., Y.M., K.E., A.T.), Graduate School of Medical Sciences, and Department of Biophysics, Faculty of Pharmaceutical Sciences (K.I., H.U.), Kyushu University, Fukuoka, Japan.
Correspondence to Hiroyuki Tsutsui, Cardiovascular Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. E-mail prehiro{at}cardiol.med.kyushu-u.ac.jp
Abstract—Experimental and clinical studies have suggested an increased production of reactive oxygen species (ROS) in the failing myocardium. The present study aimed to obtain direct evidence for increased ROS and to determine the contribution of superoxide anion (·O2-), H2O2, and hydroxy radical (·OH) in failing myocardial tissue. Heart failure was produced in adult mongrel dogs by rapid ventricular pacing at 240 bpm for 4 weeks. To assess the production of ROS directly, freeze-clamped myocardial tissue homogenates were reacted with the nitroxide radical, 4-hydroxy-2,2,6,6,-tetramethyl-piperidine-N-oxyl, and its spin signals were detected by electron spin resonance spectroscopy. The rate of electron spin resonance signal decay, proportional to ·OH level, was significantly increased in heart failure, which was inhibited by the addition of dimethylthiourea (·OH scavenger) into the reaction mixture. Increased ·OH in the failing heart was abolished to the same extent in the presence of desferrioxamine (iron chelator), catalase (H2O2 scavenger), and 4,5-dihydroxy-1,3-benzene disulfonic acid (Tiron; LaMotte) (·O2- scavenger), indicating that ·OH originated from H2O2 and ·O2-. Further, ·O2- produced in normal myocardium in the presence of antimycin A (mitochondrial complex III inhibitor) could reproduce the increase of H2O2 and ·OH seen in the failing tissue. There was a significant positive relation between myocardial ROS level and left ventricular contractile dysfunction. In conclusion, in the failing myocardium, ·OH was produced as a reactive product of ·O2- and H2O2, which might play an important role in left ventricular failure.
Key Words: antioxidant • free radicals • heart failure • myocardial contraction • myocardium
This article has been cited by other articles:
 |
|
 |
|
  N. Morita, A. A. Sovari, Y. Xie, M. C. Fishbein, W. J. Mandel, A. Garfinkel, S.-F. Lin, P.-S. Chen, L.-H. Xie, F. Chen, et al. Increased susceptibility of aged hearts to ventricular fibrillation during oxidative stress Am J Physiol Heart Circ Physiol, November 1, 2009; 297(5): H1594 - H1605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Potenza, S. Gagliardi, L. De Benedictis, A. Zigrino, E. Tiravanti, G. Colantuono, A. Federici, L. Lorusso, V. Benagiano, M. J. Quon, et al. Treatment of spontaneously hypertensive rats with rosiglitazone ameliorates cardiovascular pathophysiology via antioxidant mechanisms in the vasculature Am J Physiol Endocrinol Metab, September 1, 2009; 297(3): E685 - E694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Serpillon, B. C. Floyd, R. S. Gupte, S. George, M. Kozicky, V. Neito, F. Recchia, W. Stanley, M. S. Wolin, and S. A. Gupte Superoxide production by NAD(P)H oxidase and mitochondria is increased in genetically obese and hyperglycemic rat heart and aorta before the development of cardiac dysfunction. The role of glucose-6-phosphate dehydrogenase-derived NADPH Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H153 - H162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. N. Dedkova and L. A. Blatter Characteristics and function of cardiac mitochondrial nitric oxide synthase J. Physiol., February 15, 2009; 587(4): 851 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tsutsui, S. Kinugawa, and S. Matsushima Mitochondrial oxidative stress and dysfunction in myocardial remodelling Cardiovasc Res, February 15, 2009; 81(3): 449 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tsutsumi, T. Ide, M. Yamato, W. Kudou, M. Andou, Y. Hirooka, H. Utsumi, H. Tsutsui, and K. Sunagawa Modulation of the myocardial redox state by vagal nerve stimulation after experimental myocardial infarction Cardiovasc Res, March 1, 2008; 77(4): 713 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vecoli and N. Paolocci When the heart sleeps... Is the vagus resetting the myocardial 'redox clock'? Cardiovasc Res, March 1, 2008; 77(4): 609 - 611. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ricci, V. Pastukh, J. Leonard, J. Turrens, G. Wilson, D. Schaffer, and S. W. Schaffer Mitochondrial DNA damage triggers mitochondrial-superoxide generation and apoptosis Am J Physiol Cell Physiol, February 1, 2008; 294(2): C413 - C422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Shang, S. Sanyal, A. E. Pfahnl, Z. Jiao, J. Allen, H. Liu, and S. C. Dudley Jr. NF-{kappa}B-dependent transcriptional regulation of the cardiac scn5a sodium channel by angiotensin II Am J Physiol Cell Physiol, January 1, 2008; 294(1): C372 - C379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zhang, X. Xu, X. Hu, E. D. van Deel, G. Zhu, and Y. Chen Inducible Nitric Oxide Synthase Deficiency Protects the Heart From Systolic Overload-Induced Ventricular Hypertrophy and Congestive Heart Failure Circ. Res., April 13, 2007; 100(7): 1089 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Doerries, K. Grote, D. Hilfiker-Kleiner, M. Luchtefeld, A. Schaefer, S. M. Holland, S. Sorrentino, C. Manes, B. Schieffer, H. Drexler, et al. Critical Role of the NAD(P)H Oxidase Subunit p47phox for Left Ventricular Remodeling/Dysfunction and Survival After Myocardial Infarction Circ. Res., March 30, 2007; 100(6): 894 - 903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Madamanchi and M. S. Runge Mitochondrial Dysfunction in Atherosclerosis Circ. Res., March 2, 2007; 100(4): 460 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Berndt, C. H. Lillig, and A. Holmgren Thiol-based mechanisms of the thioredoxin and glutaredoxin systems: implications for diseases in the cardiovascular system Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1227 - H1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nojiri, T. Shimizu, M. Funakoshi, O. Yamaguchi, H. Zhou, S. Kawakami, Y. Ohta, M. Sami, T. Tachibana, H. Ishikawa, et al. Oxidative Stress Causes Heart Failure with Impaired Mitochondrial Respiration J. Biol. Chem., November 3, 2006; 281(44): 33789 - 33801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Matsushima, S. Kinugawa, T. Ide, H. Matsusaka, N. Inoue, Y. Ohta, T. Yokota, K. Sunagawa, and H. Tsutsui Overexpression of glutathione peroxidase attenuates myocardial remodeling and preserves diastolic function in diabetic heart Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2237 - H2245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Zimmet and J. M. Hare Nitroso-Redox Interactions in the Cardiovascular System Circulation, October 3, 2006; 114(14): 1531 - 1544. [Full Text] [PDF]
|
|
|
|
|
|
A. Laskowski, O. L. Woodman, A. H. Cao, G. R. Drummond, T. Marshall, D. M. Kaye, and R. H. Ritchie Antioxidant actions contribute to the antihypertrophic effects of atrial natriuretic peptide in neonatal rat cardiomyocytes Cardiovasc Res, October 1, 2006; 72(1): 112 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Hingtgen, X. Tian, J. Yang, S. M. Dunlay, A. S. Peek, Y. Wu, R. V. Sharma, J. F. Engelhardt, and R. L. Davisson Nox2-containing NADPH oxidase and Akt activation play a key role in angiotensin II-induced cardiomyocyte hypertrophy Physiol Genomics, September 14, 2006; 26(3): 180 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Murdoch, M. Zhang, A. C. Cave, and A. M. Shah NADPH oxidase-dependent redox signalling in cardiac hypertrophy, remodelling and failure Cardiovasc Res, July 15, 2006; 71(2): 208 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Song, J. C. Shryock, S. Wagner, L. S. Maier, and L. Belardinelli Blocking Late Sodium Current Reduces Hydrogen Peroxide-Induced Arrhythmogenic Activity and Contractile Dysfunction J. Pharmacol. Exp. Ther., July 1, 2006; 318(1): 214 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Matsushima, T. Ide, M. Yamato, H. Matsusaka, F. Hattori, M. Ikeuchi, T. Kubota, K. Sunagawa, Y. Hasegawa, T. Kurihara, et al. Overexpression of Mitochondrial Peroxiredoxin-3 Prevents Left Ventricular Remodeling and Failure After Myocardial Infarction in Mice Circulation, April 11, 2006; 113(14): 1779 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-s. Liang, W. Mao, C. Iwai, S. Fukuoka, and S. Y. Stevens Cardiac sympathetic neuroprotective effect of desipramine in tachycardia-induced cardiomyopathy Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H995 - H1003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ichihara, A. Noda, K. Nagata, K. Obata, J. Xu, G. Ichihara, S. Oikawa, S. Kawanishi, Y. Yamada, and M. Yokota Pravastatin increases survival and suppresses an increase in myocardial matrix metalloproteinase activity in a rat model of heart failure Cardiovasc Res, February 15, 2006; 69(3): 726 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Hajjar and J. A. Leopold Xanthine Oxidase Inhibition and Heart Failure: Novel Therapeutic Strategy for Ventricular Dysfunction? Circ. Res., February 3, 2006; 98(2): 169 - 171. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cave, D. Grieve, S. Johar, M. Zhang, and A. M Shah NADPH oxidase-derived reactive oxygen species in cardiac pathophysiology Phil Trans R Soc B, December 29, 2005; 360(1464): 2327 - 2334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nimata, T.-a. Okabe, M. Hattori, Z. Yuan, K. Shioji, and C. Kishimoto MCI-186 (edaravone), a novel free radical scavenger, protects against acute autoimmune myocarditis in rats Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2514 - H2518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Cucoranu, R. Clempus, A. Dikalova, P. J. Phelan, S. Ariyan, S. Dikalov, and D. Sorescu NAD(P)H Oxidase 4 Mediates Transforming Growth Factor-{beta}1-Induced Differentiation of Cardiac Fibroblasts Into Myofibroblasts Circ. Res., October 28, 2005; 97(9): 900 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ikeuchi, H. Tsutsui, T. Shiomi, H. Matsusaka, S. Matsushima, J. Wen, T. Kubota, and A. Takeshita Inhibition of TGF-{beta} signaling exacerbates early cardiac dysfunction but prevents late remodeling after infarction Cardiovasc Res, December 1, 2004; 64(3): 526 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Tomaselli and D. P. Zipes What Causes Sudden Death in Heart Failure? Circ. Res., October 15, 2004; 95(8): 754 - 763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Han, H. Long, H. Wang, J. Wang, Y. Zhang, and Z. Wang Progressive apoptotic cell death triggered by transient oxidative insult in H9c2 rat ventricular cells: a novel pattern of apoptosis and the mechanisms Am J Physiol Heart Circ Physiol, June 1, 2004; 286(6): H2169 - H2182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kishi, Y. Hirooka, Y. Kimura, K. Ito, H. Shimokawa, and A. Takeshita Increased Reactive Oxygen Species in Rostral Ventrolateral Medulla Contribute to Neural Mechanisms of Hypertension in Stroke-Prone Spontaneously Hypertensive Rats Circulation, May 18, 2004; 109(19): 2357 - 2362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Matsui, T. Shimosawa, K. Itakura, X. Guanqun, K. Ando, and T. Fujita Adrenomedullin Can Protect Against Pulmonary Vascular Remodeling Induced by Hypoxia Circulation, May 11, 2004; 109(18): 2246 - 2251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. Peterson, P. Herrero, K. B. Schechtman, S. B. Racette, A. D. Waggoner, Z. Kisrieva-Ware, C. Dence, S. Klein, J. Marsala, T. Meyer, et al. Effect of Obesity and Insulin Resistance on Myocardial Substrate Metabolism and Efficiency in Young Women Circulation, May 11, 2004; 109(18): 2191 - 2196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mulder, S. Barbier, A. Chagraoui, V. Richard, J. P. Henry, F. Lallemand, S. Renet, G. Lerebours, F. Mahlberg-Gaudin, and C. Thuillez Long-Term Heart Rate Reduction Induced by the Selective If Current Inhibitor Ivabradine Improves Left Ventricular Function and Intrinsic Myocardial Structure in Congestive Heart Failure Circulation, April 6, 2004; 109(13): 1674 - 1679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Galinanes and A. G Fowler Role of clinical pathologies in myocardial injury following ischaemia and reperfusion Cardiovasc Res, February 15, 2004; 61(3): 512 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shiomi, H. Tsutsui, H. Matsusaka, K. Murakami, S. Hayashidani, M. Ikeuchi, J. Wen, T. Kubota, H. Utsumi, and A. Takeshita Overexpression of Glutathione Peroxidase Prevents Left Ventricular Remodeling and Failure After Myocardial Infarction in Mice Circulation, February 3, 2004; 109(4): 544 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Czubryt and E. N. Olson Balancing Contractility and Energy Production: The Role of Myocyte Enhancer Factor 2 (MEF2) in Cardiac Hypertrophy Recent Prog. Horm. Res., January 1, 2004; 59(1): 105 - 124. [Abstract] [Full Text]
|
|
|
|
|
|
R. A. Ahokas, K. J. Warrington, I. C. Gerling, Y. Sun, L. A. Wodi, P. A. Herring, L. Lu, S. K. Bhattacharya, A. E. Postlethwaite, and K. T. Weber Aldosteronism and Peripheral Blood Mononuclear Cell Activation: A Neuroendocrine-Immune Interface Circ. Res., November 14, 2003; 93 (10): e124 - e135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wagner, T. Seidler, E. Picht, L. S Maier, V. Kazanski, N. Teucher, W. Schillinger, B. Pieske, G. Isenberg, G. Hasenfuss, et al. Na+-Ca2+ exchanger overexpression predisposes to reactive oxygen species-induced injury Cardiovasc Res, November 1, 2003; 60(2): 404 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Qin, J. Shite, and C.-s. Liang Antioxidants attenuate myocyte apoptosis and improve cardiac function in CHF: association with changes in MAPK pathways Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H822 - H832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shiomi, H. Tsutsui, M. Ikeuchi, H. Matsusaka, S. Hayashidani, N. Suematsu, J. Wen, T. Kubota, and A. Takeshita Streptozotocin-induced hyperglycemia exacerbates left ventricular remodeling and failure after experimental myocardial infarction J. Am. Coll. Cardiol., July 2, 2003; 42(1): 165 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Heymes, J. K. Bendall, P. Ratajczak, A. C. Cave, J.-L. Samuel, G. Hasenfuss, and A. M. Shah Increased myocardial NADPH oxidase activity in human heart failure J. Am. Coll. Cardiol., June 18, 2003; 41(12): 2164 - 2171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Warnholtz and T. Munzel The failing human heart: Another battlefield for the NAD(P)H oxidase? J. Am. Coll. Cardiol., June 18, 2003; 41(12): 2172 - 2174. [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shimosawa, T. Ogihara, H. Matsui, T. Asano, K. Ando, and T. Fujita Deficiency of Adrenomedullin Induces Insulin Resistance by Increasing Oxidative Stress Hypertension, May 1, 2003; 41(5): 1080 - 1085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Suematsu, H. Tsutsui, J. Wen, D. Kang, M. Ikeuchi, T. Ide, S. Hayashidani, T. Shiomi, T. Kubota, N. Hamasaki, et al. Oxidative Stress Mediates Tumor Necrosis Factor-{alpha}-Induced Mitochondrial DNA Damage and Dysfunction in Cardiac Myocytes Circulation, March 18, 2003; 107(10): 1418 - 1423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. B. Suliman, M. S. Carraway, and C. A. Piantadosi Postlipopolysaccharide Oxidative Damage of Mitochondrial DNA Am. J. Respir. Crit. Care Med., February 15, 2003; 167(4): 570 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Machida, T. Kubota, N. Kawamura, H. Funakoshi, T. Ide, H. Utsumi, Y. Y. Li, A. M. Feldman, H. Tsutsui, H. Shimokawa, et al. Overexpression of tumor necrosis factor-alpha increases production of hydroxyl radical in murine myocardium Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H449 - H455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Kaye, M. M. Parnell, and B. A. Ahlers Reduced Myocardial and Systemic L-Arginine Uptake in Heart Failure Circ. Res., December 13, 2002; 91(12): 1198 - 1203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Srivastava, B. Chandrasekar, A. Bhatnagar, and S. D. Prabhu Lipid peroxidation-derived aldehydes and oxidative stress in the failing heart: role of aldose reductase Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2612 - H2619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nomoto, T. Nishina, S. Miwa, H. Tsuneyoshi, I. Maruyama, K. Nishimura, and M. Komeda Angiotensin-Converting Enzyme Inhibitor Helps Prevent Late Remodeling After Left Ventricular Aneurysm Repair in Rats Circulation, September 24, 2002; 106(12_suppl_1): I-115 - I-119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Theilmeier, P. Verhamme, S. Dymarkowski, H. Beck, H. Bernar, M. Lox, S. Janssens, M.-C. Herregods, E. Verbeken, D. Collen, et al. Hypercholesterolemia in Minipigs Impairs Left Ventricular Response to Stress: Association With Decreased Coronary Flow Reserve and Reduced Capillary Density Circulation, August 27, 2002; 106(9): 1140 - 1146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Ballinger, C. Patterson, C. A. Knight-Lozano, D. L. Burow, C. A. Conklin, Z. Hu, J. Reuf, C. Horaist, R. Lebovitz, G. C. Hunter, et al. Mitochondrial Integrity and Function in Atherogenesis Circulation, July 30, 2002; 106(5): 544 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nakamura, K. Egashira, Y. Machida, S. Hayashidani, M. Takeya, H. Utsumi, H. Tsutsui, and A. Takeshita Probucol Attenuates Left Ventricular Dysfunction and Remodeling in Tachycardia-Induced Heart Failure: Roles of Oxidative Stress and Inflammation Circulation, July 16, 2002; 106(3): 362 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, J. H. Traverse, R. Du, M. Hou, and R. J. Bache Nitric Oxide Modulates Myocardial Oxygen Consumption in the Failing Heart Circulation, July 9, 2002; 106(2): 273 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Scheubel, B. Bartling, A. Simm, R.-E. Silber, K. Drogaris, D. Darmer, and J. Holtz Apoptotic pathway activation from mitochondria and death receptors without caspase-3 cleavage in failing human myocardium: Fragile balance of myocyte survival? J. Am. Coll. Cardiol., February 6, 2002; 39(3): 481 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bauersachs, M. Heck, D. Fraccarollo, S. K. Hildemann, G. Ertl, M. Wehling, and M. Christ Addition of spironolactone to angiotensin-converting enzyme inhibition in heart failure improves endothelial vasomotor dysfunction: Role of vascular superoxide anion formation and endothelial nitric oxide synthase expression J. Am. Coll. Cardiol., January 16, 2002; 39(2): 351 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shimosawa, Y. Shibagaki, K. Ishibashi, K. Kitamura, K. Kangawa, S. Kato, K. Ando, and T. Fujita Adrenomedullin, an Endogenous Peptide, Counteracts Cardiovascular Damage Circulation, January 1, 2002; 105(1): 106 - 111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nakamura, K. Egashira, K. Arimura, Y. Machida, T. Ide, H. Tsutsui, H. Shimokawa, and A. Takeshita Increased inactivation of nitric oxide is involved in impaired coronary flow reserve in heart failure Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2619 - H2625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Carnes, M. K. Chung, T. Nakayama, H. Nakayama, R. S. Baliga, S. Piao, A. Kanderian, S. Pavia, R. L. Hamlin, P. M. McCarthy, et al. Ascorbate Attenuates Atrial Pacing-Induced Peroxynitrite Formation and Electrical Remodeling and Decreases the Incidence of Postoperative Atrial Fibrillation Circ. Res., September 14, 2001; 89 (6): e32 - e38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bérubé, D. Caouette, and P. Daleau Hydrogen Peroxide Modifies the Kinetics of HERG Channel Expressed in a Mammalian Cell Line J. Pharmacol. Exp. Ther., April 1, 2001; 297(1): 96 - 102. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ide, H. Tsutsui, S. Hayashidani, D. Kang, N. Suematsu, K.-i. Nakamura, H. Utsumi, N. Hamasaki, and A. Takeshita Mitochondrial DNA Damage and Dysfunction Associated With Oxidative Stress in Failing Hearts After Myocardial Infarction Circ. Res., March 16, 2001; 88(5): 529 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Siwik, P. J. Pagano, and W. S. Colucci Oxidative stress regulates collagen synthesis and matrix metalloproteinase activity in cardiac fibroblasts Am J Physiol Cell Physiol, January 1, 2001; 280(1): C53 - C60. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Arimura, K. Egashira, R. Nakamura, T. Ide, H. Tsutsui, H. Shimokawa, and A. Takeshita Increased inactivation of nitric oxide is involved in coronary endothelial dysfunction in heart failure Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H68 - H75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tsutsui, T. Ide, S. Hayashidani, N. Suematsu, H. Utsumi, R. Nakamura, K. Egashira, and A. Takeshita Greater susceptibility of failing cardiac myocytes to oxygen free radical-mediated injury Cardiovasc Res, January 1, 2001; 49(1): 103 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Filippatos, B. D. Uhal, H. Tsutsui, T. Ide, S. Kinugawa, A. Takeshita, and H. Utsumi Effects of Amiodarone on Heart Cells Response Circulation, November 14, 2000; 102 (20): e170 - e170. [Full Text] [PDF]
|
|
|
|
|
|
S. Kinugawa, H. Tsutsui, S. Hayashidani, T. Ide, N. Suematsu, S. Satoh, H. Utsumi, and A. Takeshita Treatment With Dimethylthiourea Prevents Left Ventricular Remodeling and Failure After Experimental Myocardial Infarction in Mice : Role of Oxidative Stress Circ. Res., September 1, 2000; 87(5): 392 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Sawyer and W. S. Colucci Mitochondrial Oxidative Stress in Heart Failure : "Oxygen Wastage" Revisited Circ. Res., February 4, 2000; 86(2): 119 - 120. [Full Text] [PDF]
|
|
|
|
|
|
G. D. Thomas, W. Zhang, and R. G. Victor Impaired Modulation of Sympathetic Vasoconstriction in Contracting Skeletal Muscle of Rats With Chronic Myocardial Infarctions : Role of Oxidative Stress Circ. Res., April 27, 2001; 88(8): 816 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Akao, A. Ohler, B. O'Rourke, and E. Marban Mitochondrial ATP-Sensitive Potassium Channels Inhibit Apoptosis Induced by Oxidative Stress in Cardiac Cells Circ. Res., June 22, 2001; 88(12): 1267 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cesselli, I. Jakoniuk, L. Barlucchi, A. P. Beltrami, T. H. Hintze, B. Nadal-Ginard, J. Kajstura, A. Leri, and P. Anversa Oxidative Stress-Mediated Cardiac Cell Death Is a Major Determinant of Ventricular Dysfunction and Failure in Dog Dilated Cardiomyopathy Circ. Res., August 3, 2001; 89(3): 279 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Pimentel, J. K. Amin, L. Xiao, T. Miller, J. Viereck, J. Oliver-Krasinski, R. Baliga, J. Wang, D. A. Siwik, K. Singh, et al. Reactive Oxygen Species Mediate Amplitude-Dependent Hypertrophic and Apoptotic Responses to Mechanical Stretch in Cardiac Myocytes Circ. Res., August 31, 2001; 89(5): 453 - 460. [Abstract] [Full Text] [PDF]
|
|