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(Circulation Research. 2000;86:e110.)
© 2000 American Heart Association, Inc.

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Nuclear Factor-B and cAMP Response Element Binding Protein Mediate Opposite Transcriptional Effects on the Flk-1/KDR Gene Promoter

Barbara Illi, PierLorenzo Puri, Liliana Morgante, Maurizio C. Capogrossi, Carlo Gaetano

From the Laboratorio di Patologia Vascolare, Istituto Dermopatico dell’Immacolata (B.I., L.M., M.C.C., C.G.), Rome, Italy; Department of Biology, Center for Molecular Genetics (P.L.P.), University of California San Diego, La Jolla, Calif.

Correspondence to Dr Carlo Gaetano, Laboratorio di Patologia Vascolare, Istituto Dermopatico dell’Immacolata, Via dei Monti di Creta 104, 00167 Rome, Italy. E-mail gaetano{at}idi.it

Abstract—The vascular endothelial growth factor receptor Flk-1/KDR is highly expressed during development and almost disappears in adult tissues. Despite its biological relevance, little is known about the molecular mechanisms controlling its expression. In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-B (NF-B)–related antigens bind specific sequences in the Flk-1/KDR promoter. Functional studies demonstrate that cAMP represses whereas tumor necrosis factor-, an activator of NF-B, stimulates promoter activity. Histone acetyltransferases (HATs) P/CAF and CBP/p300 together with p65/RelA, the catalytic subunit of NF-B, increase Flk-1/KDR promoter activity 10- to 20-fold. Consistently, inhibition by cAMP is reverted by increasing intracellular HATs and is completely abolished by site-specific mutagenesis of the cAMP response element. In contrast, specific mutations in the NF-B response element abolish responsiveness to p65/RelA and HATs without affecting cAMP-dependent repression. These results suggest that opposing signaling pathways, activating NF-B or CREB and requiring HAT molecules, control Flk-1/KDR promoter activity. The full text of this article is available at http://www.circresaha.org.

Key Words: vascular endothelial growth factor receptor • promoter • nuclear factor-B • transcription • angiogenesis

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