© 2000 American Heart Association, Inc.
Molecular Medicine |
Immunoneutralization of Glycoprotein Ib
Attenuates Endotoxin-Induced Interactions of Platelets and Leukocytes With Rat Venular Endothelium In Vivo
From the Departments of Biochemistry (Y. Ishimura, M.S.) and Medicine (Y. Ikeda) and Blood Center (M.H.), School of Medicine, Keio University, Tokyo; the Department of Obstetrics and Gynecology, Ehime University School of Medicine, Ehime (T.K., M.I.); and the Pharmaceutical Frontier Research Laboratories, JT Inc, Yokohama, Kanagawa (T.T., S.S.), Japan.
Correspondence to Makoto Suematsu, MD, PhD, Associate Professor, Department of Biochemistry, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. E-mail msuem{at}mc.med.keio.ac.jp
Abstract—This study aimed to
examine molecular mechanisms for endotoxin-induced adhesive changes in
platelets in vivo. Platelets labeled with
carboxyfluorescein diacetate succinimidyl ester were
visualized in rat mesenteric venules through intravital microscopy
assisted by a high-speed fluorescence video imager at 1000
frames per second or by a normal-speed intensifier under monitoring of
erythrocyte velocity. Leukocyte rolling was examined by normal-speed
transmission video images. The velocity of platelets traveling
along the centerline of venules followed that of erythrocytes, whereas
that measured at the periendothelial space was
significantly smaller than the erythrocyte velocity; a majority of
these cells exhibited transient but notable rolling with
endothelium. Administration of endotoxin increased the
density of periendothelial platelets and reduced
the rolling velocities of platelets and leukocytes in venules: All
events were attenuated by anti–rat P-selectin monoclonal antibody
s789G or by anti–human glycoprotein (GP) Ib
monoclonal antibody
GUR83/35, which blocks ristocetin-induced aggregation of rat
platelets. Isolated rat platelets injected into
endotoxin-pretreated rats were able to roll on the venules. This event
was attenuated by pretreatment of platelets in vitro with GUR83/35
but not with s789G, suggesting involvement of
endothelial P-selectin and platelet GP Ib in the
endotoxin-induced responses. Furthermore, isolated human platelets
showed similar rolling interactions with endotoxin-preexposed rat
venules, and pretreatment of the platelets with GUR83/35, but not
with s789G, significantly reduced such interactions. Our results
provide the first evidence for involvement of GP Ib in
endotoxin-induced microvascular rolling of platelets and
leukocytes, and this system serves as a potentially useful tool to
examine GP Ib–associated function of human platelets in
vivo.
Key Words: platelets • endothelial cells • P-selectin • shear stress • endotoxin
This article has been cited by other articles:
 |
|
 |
|
  K. N. Patel, S. H. Soubra, R. V. Bellera, J.-F. Dong, C. A. McMullen, A. R. Burns, and R. E. Rumbaut Differential Role of von Willebrand Factor and P-Selectin on Microvascular Thrombosis in Endotoxemia Arterioscler Thromb Vasc Biol, December 1, 2008; 28(12): 2225 - 2230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. He, H. Zhang, L. Zhu, Y. Jiang, and X. Zhou Leukocyte-platelet aggregate adhesion and vascular permeability in intact microvessels: role of activated endothelial cells Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H591 - H599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-l. Stahl, M. Svensson, M. Morgelin, C. Svanborg, P. I. Tarr, J. C. Mooney, S. L. Watkins, R. Johnson, and D. Karpman Lipopolysaccharide from enterohemorrhagic Escherichia coli binds to platelets through TLR4 and CD62 and is detected on circulating platelets in patients with hemolytic uremic syndrome Blood, July 1, 2006; 108(1): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Miyao, Y. Suzuki, K. Takeshita, H. Kudo, M. Ishii, R. Hiraoka, K. Nishio, T. Tamatani, S. Sakamoto, M. Suematsu, et al. Various adhesion molecules impair microvascular leukocyte kinetics in ventilator-induced lung injury Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1059 - L1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Pitchford, S. Momi, S. Giannini, L. Casali, D. Spina, C. P. Page, and P. Gresele Platelet P-selectin is required for pulmonary eosinophil and lymphocyte recruitment in a murine model of allergic inflammation Blood, March 1, 2005; 105(5): 2074 - 2081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gotoh, J.-i. Suzuki, H. Kosuge, T. Kakuta, S. Sakamoto, M. Yoshida, and M. Isobe E-Selectin Blockade Decreases Adventitial Inflammation and Attenuates Intimal Hyperplasia in Rat Carotid Arteries After Balloon Injury Arterioscler Thromb Vasc Biol, November 1, 2004; 24(11): 2063 - 2068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Wood, R. P. Hebbel, and D. N. Granger Endothelial cell P-selectin mediates a proinflammatory and prothrombogenic phenotype in cerebral venules of sickle cell transgenic mice Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1608 - H1614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. C. Aird The Hematologic System as a Marker of Organ Dysfunction in Sepsis Mayo Clin. Proc., July 1, 2003; 78(7): 869 - 881. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Katayama, Y. Kusanagi, M. Kiyomura, H. Ochi, and M. Ito Leukocyte behaviour and permeability in the rat mesenteric microcirculation following induction of ovulation Hum. Reprod., June 1, 2003; 18(6): 1179 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. Aird The role of the endothelium in severe sepsis and multiple organ dysfunction syndrome Blood, May 15, 2003; 101(10): 3765 - 3777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Berndt Induction of Platelet-Endothelial Interactions in Postcapillary Venules in Hypercholesterolemia: Critical Role of P-Selectin Arterioscler Thromb Vasc Biol, April 1, 2003; 23(4): 525 - 527. [Full Text] [PDF]
|
|
|
|
|
|
W. H. Cerwinka, D. Cooper, C. F. Krieglstein, C. R. Ross, J. M. McCord, and D. N. Granger Superoxide mediates endotoxin-induced platelet-endothelial cell adhesion in intestinal venules Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H535 - H541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Russell, D. Cooper, A. Tailor, K. Y. Stokes, and D. N. Granger Low venular shear rates promote leukocyte-dependent recruitment of adherent platelets Am J Physiol Gastrointest Liver Physiol, January 1, 2003; 284(1): G123 - G129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Warkentin, W. C. Aird, and J. H. Rand Platelet-Endothelial Interactions: Sepsis, HIT, and Antiphospholipid Syndrome Hematology, January 1, 2003; 2003(1): 497 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kashiwagi, M. Kajimura, Y. Yoshimura, and M. Suematsu Nonendothelial Source of Nitric Oxide in Arterioles But Not in Venules: Alternative Source Revealed In Vivo by Diaminofluorescein Microfluorography Circ. Res., December 13, 2002; 91 (12): e55 - e64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Cerwinka, D. Cooper, C. F. Krieglstein, M. Feelisch, and D. N. Granger Nitric oxide modulates endotoxin-induced platelet-endothelial cell adhesion in intestinal venules Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H1111 - H1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shen, J.-f. Dong, G. M. Romo, W. Arceneaux, A. Aprico, E. E. Gardiner, J. A. Lopez, M. C. Berndt, and R. K. Andrews Functional analysis of the C-terminal flanking sequence of platelet glycoprotein Ibalpha using canine-human chimeras Blood, January 1, 2002; 99(1): 145 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nishijima, J. Kiryu, A. Tsujikawa, M. Honjo, A. Nonaka, K. Yamashiro, H. Tanihara, S. J. Tojo, Y. Ogura, and Y. Honda In Vivo Evaluation of Platelet-Endothelial Interactions after Transient Retinal Ischemia Invest. Ophthalmol. Vis. Sci., August 1, 2001; 42(9): 2102 - 2109. [Abstract] [Full Text] [PDF]
|
|