© 1999 American Heart Association, Inc.
Cellular Biology |
11,12-Epoxyeicosatrienoic Acid Stimulates Endogenous Mono-ADP-Ribosylation in Bovine Coronary Arterial Smooth Muscle
From the Departments of Pharmacology and Toxicology (P.-L.L., C.-L.C., W.B.C.), Medical College of Wisconsin, Milwaukee, Wis; Department of Medicine (R.B.), University of Massachusetts Medical Center, Worcester, Mass.
Correspondence and reprint requests to Pin-Lan Li, MD, PhD, Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226. E-mail pli{at}post.its.mcw.edu
Abstract—The role of
endogenous ADP-ribosylation in mediating the activation of
the Ca2+-activated K+ channels was
determined in bovine coronary arteries. Endogenous
ADP-ribosylation was examined by incubating coronary
arterial homogenates or lysates of cultured
coronary arterial smooth muscle cells with
[adenylate-32P]NAD. Four 32P-labeled
proteins were observed at 51, 52, 80, and 124 kDa in the
homogenates and lysates. This reaction was enhanced by the
addition of 11,12-epoxyeicosatrienoic acid (11,12-EET), a cytochrome
P450–derived eicosanoid, and GTP to the incubation. By Western blot
analysis, 42- and 70-kDa proteins were recognized by specific
antibodies against ADP-ribosyltransferase in the coronary
arterial homogenates and smooth muscle cell
lysate but not in the lysate of endothelial cells. The
52-kDa acceptor protein of endogenous ADP-ribosylation
comigrated with a protein ADP-ribosylated by cholera toxin and was
recognized and immunoprecipitated by an anti–GS
antibody. These results suggest that GS is one of
several acceptors of the ADP-ribose moiety. As shown by the patch-clamp
technique, 11,12-EET stimulated the activation of the K+
channels in the smooth muscle cells, and this activation was completely
blocked by novobiocin, vitamin K1, 3-aminobenzamide, and
m-iodobenzylguanidine, inhibitors of
endogenous mono-ADP-ribosyltransferases. We conclude that
endogenous mono-ADP-ribosyltransferases are present in
smooth muscle from bovine coronary arteries. These enzymes
transfer ADP-ribose to the cellular proteins such as GS
and may mediate intracellular signal transduction in coronary
vascular smooth muscle. In the coronary circulation, the
ADP-ribosylation signaling pathway may play an important role in
mediating the activation of the K+ channels induced by
11,12-EET.
Key Words: mono-ADP-ribosyltransferase • K+ channel • coronary artery • eicosanoid • epoxyeicosatrienoic acid
This article has been cited by other articles:
 |
|
 |
|
  B. T. Larsen, D. X. Zhang, and D. D. Gutterman Epoxyeicosatrienoic Acids, TRP Channels, and Intracellular Ca2+ in the Vasculature: An Endothelium-Derived Endothelium-Hyperpolarizing Factor? Arterioscler Thromb Vasc Biol, December 1, 2007; 27(12): 2496 - 2498. [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yang, B. B. Holmes, V. R. Gopal, R. V. K. Kishore, B. Sangras, X.-Y. Yi, J. R. Falck, and W. B. Campbell Characterization of 14,15-Epoxyeicosatrienoyl-Sulfonamides as 14,15-Epoxyeicosatrienoic Acid Agonists: Use for Studies of Metabolism and Ligand Binding J. Pharmacol. Exp. Ther., June 1, 2007; 321(3): 1023 - 1031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Spector and A. W. Norris Action of epoxyeicosatrienoic acids on cellular function Am J Physiol Cell Physiol, March 1, 2007; 292(3): C996 - C1012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Campbell and J. R. Falck Arachidonic Acid Metabolites as Endothelium-Derived Hyperpolarizing Factors Hypertension, March 1, 2007; 49(3): 590 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lu, D. Ye, X. Wang, J. M. Seubert, J. P. Graves, J. A. Bradbury, D. C. Zeldin, and H.-C. Lee Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms J. Physiol., September 1, 2006; 575(2): 627 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dhanasekaran, R. Al-Saghir, B. Lopez, D. Zhu, D. D. Gutterman, E. R. Jacobs, and M. Medhora Protective effects of epoxyeicosatrienoic acids on human endothelial cells from the pulmonary and coronary vasculature Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H517 - H531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zhang, G. Zhang, A. Y. Zhang, M. J. Koeberl, E. Wallander, and P.-L. Li Production of NAADP and its role in Ca2+ mobilization associated with lysosomes in coronary arterial myocytes Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H274 - H282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Carroll, A. B. Doumad, J. Li, M. K. Cheng, J. R. Falck, and J. C. McGiff Adenosine2A receptor vasodilation of rat preglomerular microvessels is mediated by EETs that activate the cAMP/PKA pathway Am J Physiol Renal Physiol, July 1, 2006; 291(1): F155 - F161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ye, W. Zhou, T. Lu, S. G. Jagadeesh, J. R. Falck, and H.-C. Lee Mechanism of rat mesenteric arterial KATP channel activation by 14,15-epoxyeicosatrienoic acid Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1326 - H1336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Campbell, B. B. Holmes, J. R. Falck, J. H. Capdevila, and K. M. Gauthier Regulation of potassium channels in coronary smooth muscle by adenoviral expression of cytochrome P-450 epoxygenase Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H64 - H71. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zolkiewska Ecto-ADP-ribose Transferases: Cell-Surface Response to Local Tissue Injury Physiology, December 1, 2005; 20(6): 374 - 381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Gross, J. R. Falck, E. R. Gross, M. Isbell, J. Moore, and K. Nithipatikom Cytochrome P450 and arachidonic acid metabolites: Role in myocardial ischemia/reperfusion injury revisited Cardiovasc Res, October 1, 2005; 68(1): 18 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. R. Michaelis, J. R. Falck, R. Schmidt, R. Busse, and I. Fleming Cytochrome P4502C9-Derived Epoxyeicosatrienoic Acids Induce the Expression of Cyclooxygenase-2 in Endothelial Cells Arterioscler Thromb Vasc Biol, February 1, 2005; 25(2): 321 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-F. Xiao, Q. Ke, J. M. Seubert, J. A. Bradbury, J. Graves, L. M. DeGraff, J. R. Falck, K. Krausz, H. V. Gelboin, J. P. Morgan, et al. Enhancement of Cardiac L-Type Ca2+ Currents in Transgenic Mice with Cardiac-Specific Overexpression of CYP2J2 Mol. Pharmacol., December 1, 2004; 66(6): 1607 - 1616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. X. Zhang, A.-P. Zou, and P.-L. Li Ceramide-induced activation of NADPH oxidase and endothelial dysfunction in small coronary arteries Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H605 - H612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Falck, U. M. Krishna, Y. K. Reddy, P. S. Kumar, K. M. Reddy, S. B. Hittner, C. Deeter, K. K. Sharma, K. M. Gauthier, and W. B. Campbell Comparison of vasodilatory properties of 14,15-EET analogs: structural requirements for dilation Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H337 - H349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, N. L. Weintraub, C. L. Oltman, L. L. Stoll, T. L. Kaduce, S. Harmon, K. C. Dellsperger, C. Morisseau, B. D. Hammock, and A. A. Spector Human coronary endothelial cells convert 14,15-EET to a biologically active chain-shortened epoxide Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2306 - H2314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Snyder, U. M. Krishna, J. R. Falck, and A. A. Spector Evidence for a membrane site of action for 14,15-EET on expression of aromatase in vascular smooth muscle Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1936 - H1942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sun, X. Sui, J. A. Bradbury, D. C. Zeldin, M. S. Conte, and J. K. Liao Inhibition of Vascular Smooth Muscle Cell Migration by Cytochrome P450 Epoxygenase-Derived Eicosanoids Circ. Res., May 17, 2002; 90(9): 1020 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Potente, U. R. Michaelis, B. Fisslthaler, R. Busse, and I. Fleming Cytochrome P450 2C9-induced Endothelial Cell Proliferation Involves Induction of Mitogen-activated Protein (MAP) Kinase Phosphatase-1, Inhibition of the c-Jun N-terminal Kinase, and Up-regulation of Cyclin D1 J. Biol. Chem., May 3, 2002; 277(18): 15671 - 15676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-X. Tang, Y.-F. Chen, A.-P. Zou, W. B. Campbell, and P.-L. Li Role of FKBP12.6 in cADPR-induced activation of reconstituted ryanodine receptors from arterial smooth muscle Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1304 - H1310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Harder, C. Zhang, and D. Gebremedhin Astrocytes Function in Matching Blood Flow to Metabolic Activity Physiology, February 1, 2002; 17(1): 27 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lauterbach, E. Barbosa-Sicard, M.-H. Wang, H. Honeck, E. Kargel, J. Theuer, M. L. Schwartzman, H. Haller, F. C. Luft, M. Gollasch, et al. Cytochrome P450-Dependent Eicosapentaenoic Acid Metabolites Are Novel BK Channel Activators Hypertension, February 1, 2002; 39(2): 609 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Roman P-450 Metabolites of Arachidonic Acid in the Control of Cardiovascular Function Physiol Rev, January 1, 2002; 82(1): 131 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zou, Q. Yang, A. P.C. Yim, and G.-W. He Epoxyeicosatrienoic acids (EET11,12) may partially restore endothelium-derived hyperpolarizing factor-mediated function in coronary microarteries Ann. Thorac. Surg., December 1, 2001; 72(6): 1970 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Fleming Cytochrome P450 and Vascular Homeostasis Circ. Res., October 26, 2001; 89(9): 753 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Pomposiello, M. A. Carroll, J. R. Falck, and J. C. McGiff Epoxyeicosatrienoic Acid-Mediated Renal Vasodilation to Arachidonic Acid Is Enhanced in SHR Hypertension, March 1, 2001; 37(3): 887 - 893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fukao, H. S. Mason, J. L. Kenyon, B. Horowitz, and K. D. Keef Regulation of BKca Channels Expressed in Human Embryonic Kidney 293 Cells by Epoxyeicosatrienoic Acid Mol. Pharmacol., January 1, 2001; 59(1): 16 - 23. [Abstract] [Full Text]
|
|
|
|
|
|
J. Geiger, A.-P. Zou, W. B. Campbell, and P.-L. Li Inhibition of cADP-Ribose Formation Produces Vasodilation in Bovine Coronary Arteries Hypertension, January 1, 2000; 35(1): 397 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Node, X.-L. Ruan, J. Dai, S.-X. Yang, L. Graham, D. C. Zeldin, and J. K. Liao Activation of Galpha s Mediates Induction of Tissue-type Plasminogen Activator Gene Transcription by Epoxyeicosatrienoic Acids J. Biol. Chem., May 4, 2001; 276(19): 15983 - 15989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Zeldin Epoxygenase Pathways of Arachidonic Acid Metabolism J. Biol. Chem., September 21, 2001; 276(39): 36059 - 36062. [Full Text] [PDF]
|
|
|
|
|
|
P.-L. Li, D. X. Zhang, Z.-D. Ge, and W. B. Campbell Role of ADP-ribose in 11,12-EET-induced activation of KCa channels in coronary arterial smooth muscle cells Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1229 - H1236. [Abstract] [Full Text] [PDF]
|
|