© 1999 American Heart Association, Inc.
Original Contribution |
Downregulation of Connexin 45 Gene Products During Mouse Heart Development
From the Laboratoire de Génétique et Physiologie du Développement (S.A., M.T-R., T.J-G., I.M., E.T., J-P.C., D.B.G.), Institut de Biologie du Développement de Marseille, CNRS/INSERM/AP Marseille/Université de la Méditerranée, Campus de Luminy, Marseille, France; Department of Anatomy and Embryology (A.F.M.M., W.H.L.), Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands; and Laboratoire d'Immunochimie des Peptides et des Virus (J-P.B.), Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France. The current affiliation for E.T. is the Centre for Genome Research, University of Edinburgh, United Kingdom.
Correspondence to Daniel Gros, LGPD-IBDM, Campus de Luminy, Case 907, 13288 Marseille Cédex 09, France. E-mail gros{at}lgpd.univ-mrs.fr
Abstract—The electrical activity in heart is generated in the sinoatrial node and then propagates to the atrial and ventricular tissues. The gap junction channels that couple the myocytes are responsible for this propagation process. The gap junction channels are dodecamers of transmembrane proteins of the connexin (Cx) family. Three members of this family have been demonstrated to be synthesized in the cardiomyocytes: Cx40, Cx43, and Cx45. In addition, each of them has been shown to form channels with unique and specific electrophysiological properties. Understanding the conduction phenomenon requires detailed knowledge of the spatiotemporal expression pattern of these Cxs in heart. The expression patterns of Cx40 and Cx43 have been previously described in the adult heart and during its development. Here we report the expression of Cx45 gene products in mouse heart from the stage of the first contractions (8.5 days postcoitum [dpc]) to the adult stage. The Cx45 gene transcript was demonstrated by reverse transcriptase–polymerase chain reaction experiments to be present in heart at all stages investigated. Between 8.5 and 10.5 dpc it was shown by in situ hybridization to be expressed in low amounts in all cardiac compartments (including the inflow and outflow tracts and the atrioventricular canal) and then to be downregulated from 11 to 12 dpc onward. At subsequent fetal stages, the transcript was weakly detected in the ventricles, with the most distinct expression in the outflow tract. Cx45 protein was demonstrated by immunofluorescence microscopy to be expressed in the myocytes of young embryonic hearts (8.5 to 9.5 dpc). However, beyond 10.5 dpc the protein was no longer detected with this technique in the embryonic, fetal, or neonatal working myocardium, although it could be shown by immunoblotting that the protein was still synthesized in neonatal heart. In the major part of adult heart, Cx45 was undetectable. It was, however, clearly seen in the anterior regions of the interventricular septum and in trace amounts in some small foci dispersed in the ventricular free walls. Cx45 gene is the first Cx gene so far demonstrated to be activated in heart at the stage of the first contractions. The coordination of myocytes during the slow peristaltic contractions that occur at this stage would thus appear to be controlled by the Cx45 channels.
Key Words: connexin 45 • heart • development
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