© 1998 American Heart Association, Inc.
Original Contributions |
Embryonic Expression Suggests an Important Role for CRP2/SmLIM in the Developing Cardiovascular System
From the Cardiovascular Biology Laboratory, Harvard School of Public Health (M.K.J., S.K., C.-M.H., M.D.L., S.-F.Y., N.E.S.S., M.T.C., M.W.F., E.H., M.-E.L.), the Department of Medicine, Harvard Medical School (M.K.J., N.E.S.S., M.T.C., R.L.M., E.H., M.-E.L.), and the Cardiovascular Division of the Department of Medicine (M.K.J., N.E.S.S., M.T.C., M.-E.L.) and the Department of Genetics (I.W., R.L.M.), Brigham and Women's Hospital, Boston, Mass.
Correspondence to Mu-En Lee, MD, PhD, Cardiovascular Biology Laboratory, Harvard School of Public Health, 677 Huntington Ave, Boston, MA 02115. E-mail lee{at}cvlab.harvard.edu
Abstract—Proteins of the LIM family are critical regulators of development and differentiation in various cell types. We have described the cloning of cysteine-rich protein 2/smooth muscle LIM protein (CRP2/SmLIM), a LIM-only protein expressed in differentiated vascular smooth muscle cells. As a first step toward understanding the potential functions of CRP2/SmLIM, we analyzed its expression after gastrulation in developing mice and compared the expression of CRP2/SmLIM with that of the other 2 members of the CRP subclass, CRP1 and CRP3/MLP. In situ hybridization in whole-mount and sectioned embryos showed that CRP2/SmLIM was expressed in the sinus venosus and the 2 cardiac chambers at embryonic day 9. Vascular expression of CRP2/SmLIM was first seen at embryonic day 10. At subsequent time points, CRP2/SmLIM expression decreased in the heart but remained high in the vasculature. CRP1 was expressed both in vascular and nonvascular tissues containing smooth muscle cells, whereas CRP3/MLP was expressed only in tissues containing striated muscle. These patterns of expression were maintained in the adult animal and suggest an important role for this gene family in the development of smooth and striated muscle.
Key Words: muscle, cardiac • muscle, smooth • development, mouse • hybridization, in situ • protein, zinc-finger
This article has been cited by other articles:
 |
|
 |
|
  L. C. G. Campos, A. A. Miyakawa, V. G. Barauna, L. Cardoso, T. F. Borin, L. A. d. O. Dallan, and J. E. Krieger Induction of CRP3/MLP expression during vein arterialization is dependent on stretch rather than shear stress Cardiovasc Res, July 1, 2009; 83(1): 140 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. van Tuyn, D. A. Pijnappels, A. A. F. de Vries, I. de Vries, I. van der Velde-van Dijke, S. Knaan-Shanzer, A. van der Laarse, M. J. Schalij, and D. E. Atsma Fibroblasts from human postmyocardial infarction scars acquire properties of cardiomyocytes after transduction with a recombinant myocardin gene FASEB J, October 1, 2007; 21(12): 3369 - 3379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-r. Kim-Kaneyama, W. Suzuki, K. Ichikawa, T. Ohki, Y. Kohno, M. Sata, K. Nose, and M. Shibanuma Uni-axial stretching regulates intracellular localization of Hic-5 expressed in smooth-muscle cells in vivo J. Cell Sci., March 1, 2005; 118(5): 937 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Dorrell, E. Aguilar, C. Weber, and M. Friedlander Global Gene Expression Analysis of the Developing Postnatal Mouse Retina Invest. Ophthalmol. Vis. Sci., March 1, 2004; 45(3): 1009 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-F. Chang, J. Wei, X. Liu, Y.-H. Chen, M. D. Layne, and S.-F. Yet Identification of a CArG-independent region of the cysteine-rich protein 2 promoter that directs expression in the developing vasculature Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1675 - H1683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Kumar and G. K. Owens Combinatorial Control of Smooth Muscle-Specific Gene Expression Arterioscler Thromb Vasc Biol, May 1, 2003; 23(5): 737 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Henderson, D. Brown, J. A. Richardson, E. N. Olson, and M. C. Beckerle Expression of the Gene Encoding the LIM Protein CRP2: A Developmental Profile J. Histochem. Cytochem., January 1, 2002; 50(1): 107 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kirchner, K. A. Forbush, and M. J. Bevan Identification and Characterization of Thymus LIM Protein: Targeted Disruption Reduces Thymus Cellularity Mol. Cell. Biol., December 15, 2001; 21(24): 8592 - 8604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Oettgen Transcriptional Regulation of Vascular Development Circ. Res., August 31, 2001; 89(5): 380 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kong, J. M. Shelton, B. Rothermel, X. Li, J. A. Richardson, R. Bassel-Duby, and R. S. Williams Cardiac-Specific LIM Protein FHL2 Modifies the Hypertrophic Response to {beta}-Adrenergic Stimulation Circulation, June 5, 2001; 103(22): 2731 - 2738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Zolk, P. Caroni, and M. Bohm Decreased Expression of the Cardiac LIM Domain Protein MLP in Chronic Human Heart Failure Circulation, June 13, 2000; 101(23): 2674 - 2677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Chin, K. Maemura, S. Fukumoto, M. K. Jain, M. D. Layne, M. Watanabe, C.-M. Hsieh, and M.-E. Lee Cardiovascular Basic Helix Loop Helix Factor 1, a Novel Transcriptional Repressor Expressed Preferentially in the Developing and Adult Cardiovascular System J. Biol. Chem., February 25, 2000; 275(9): 6381 - 6387. [Abstract] [Full Text] [PDF]
|
|