© 1998 American Heart Association, Inc.
Original Contributions |
Thy-1, a Novel Marker for Angiogenesis Upregulated by Inflammatory Cytokines
From the Cardiovascular Biology Laboratory, Harvard School of Public Health (W.-S.L., M.K.J., B.M.A., D.Z., S.K., K.M., S.-L.L., M.-E.L., E.H.), the Department of Medicine, Harvard Medical School (N.K.H., M.-E.L., E.H.), and the Divisions of Cardiology (M.K.J., M.-E.L.) and Radiology (N.K.H.), Brigham and Women's Hospital, Boston, Mass, and the Department of Chemistry, National Cheng Kung University (S.-Y.S.), Tainan, Taiwan, ROC.
Correspondence to Mu-En Lee, MD, PhD, Cardiovascular Biology Laboratory, Harvard School of Public Health, 677 Huntington Ave, Boston, MA 02115. E-mail lee{at}cvlab.harvard.edu
Abstract—We identified the cell
surface glycoprotein Thy-1 on the
endothelium of newly formed blood vessels in four
models of angiogenesis in adult rats. Anti–Thy-1 staining showed that
Thy-1 was upregulated in adventitial blood vessels after balloon injury
to the carotid artery. Preabsorption with a rat Thy-1–Ig fusion
construct eliminated all immunoreactivity and thus confirmed the
specificity of the Thy-1 staining. Thy-1 was also expressed in the
endothelium of small blood vessels formed after tumor
implantation in the cornea, in periureteral vessels formed after
ligation of the renal artery, and in small blood vessels of the uterus
formed during pregnancy. In contrast with its expression during adult
angiogenesis, Thy-1 was not expressed in the
endothelium of blood vessels during embryonic
angiogenesis. In vitro, the inflammatory cytokines
interleukin-1ß and tumor necrosis factor-
upregulated Thy-1 mRNA
by 8- and 14-fold, respectively. Vascular endothelial
growth factor, basic fibroblast growth factor, transforming growth
factor-ß, and platelet-derived growth factor-BB had no effect on
Thy-1 mRNA. Thus, Thy-1 appears to be a marker of adult but not
embryonic angiogenesis. The upregulation of Thy-1 by cytokines
but not growth factors indicates the importance of inflammation in the
pathogenesis of adult angiogenesis.
Key Words: endothelium • interleukin-1 • tumor necrosis factor • inflammation • angiogenesis
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