© 1998 American Heart Association, Inc.
Original Contributions |
Mitogenic Effect of Angiotensin II on Rat Carotid Arteries and Type II or III Mesenteric Microvessels but Not Type I Mesenteric Microvessels Is Mediated by Endogenous Basic Fibroblast Growth Factor
From the Department of Pathology (E.J.S., D.M.L., M.A.R., S.M.S.), University of Washington, Seattle; the Department of Pathology (J.W.), Wayne State University, School of Medicine, Detroit, Mich; and the University of Maastricht (the Netherlands) (M.J.A.P.D.).
Correspondence to E.J. Su, Department of Pathology, Box 357335, Seattle WA 98195. E-mail enmingsu{at}u.washington.edu
Abstract—In this study, anti–basic fibroblast growth factor (anti-bFGF) antibody was used to determine whether the mitogenic effect of angiotensin II in vivo could be blocked by neutralizing bFGF in the vessel wall. Animals, divided into six experimental groups, were given (1) angiotensin II, (2) angiotensin II+anti-bFGF antibody, (3) angiotensin II+normal goat IgG (ngIgG), (4) anti-bFGF antibody, (5) ngIgG, and (6) Ringer's solution. Angiotensin II at 435 ng · kg-1 · min-1 was infused into rats continuously for 1 week to induce smooth muscle cell replication, and anti-bFGF antibody or ngIgG was injected intravenously 4 times over the 1-week period at a dose of 60 mg/injection. Bromodeoxyuridine (30 mg/mL) was also continuously infused during the 1-week period. The left carotid artery of all animals was balloon-injured on day 4 of the treatment, and all groups were killed for study on day 7. The results showed that angiotensin II significantly stimulated smooth muscle replication in the balloon-injured carotid artery, intact carotid artery, and three branch levels of the mesenteric vascular tree. Anti-bFGF was able to block the mitogenic effect of angiotensin II in larger vessels but not the smallest (type I) microvessels of the mesenteric arterial tree. This differential response may be attributable to the nature of the lesions in type I vessels versus larger vessels: the type I vascular lesion has a large component of proliferating macrophages, whereas the larger vessels show less injury, few macrophages, and varying levels of smooth muscle replication. Our data suggest that the vessel wall remodeling in the angiotensin II–treated larger vessels involves DNA replication that is dependent on the presence of bFGF.
Key Words: angiotensin II • basic fibroblast growth factor • rat • smooth muscle cell
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