© 1997 American Heart Association, Inc.
Articles |
Molecular Mechanisms of Anoxia/Reoxygenation-Induced Neutrophil Adherence to Cultured Endothelial Cells
From the Departments of Physiology and Medicine (H.I., S.F., D.N.G., T.Y.A.) and the Center of Excellence in Arthritis and Rheumatism (R.E.W.), LSU Medical Center, Shreveport, La; the A.C. Burton Vascular Research Laboratory (P.R.K.), Victoria Hospital Research Institute, London, Ontario, Canada; and the First Department of Internal Medicine (T.Y.), Kyoto (Japan) Prefectural University of Medicine.
Correspondence to Dr Tak Yee Aw, Department of Physiology, LSU Medical Center, 1501 Kings Highway, Shreveport, LA 71130-3932. E-mail taw{at}lsumc.edu
Abstract The objectives of this study were to (1) determine
the time course of neutrophil adhesion to monolayers of human umbilical
vein endothelial cells (HUVECs) that were exposed to 60
minutes of anoxia followed by 30 to 600 minutes of
reoxygenation and (2) define the mechanisms responsible
for both the early (minutes) and late (hours) hyperadhesivity of
postanoxic HUVECs to human neutrophils. The results clearly demonstrate
that anoxia/reoxygenation (A/R) leads to a biphasic
increase in neutrophil adhesion to HUVECs, with peak responses
occurring at 30 minutes (phase 1) and 240 minutes (phase 2) after
reoxygenation. Oxypurinol and catalase inhibited
phase-1 adhesion, suggesting a role for xanthine oxidase and
H2O2. In comparison, platelet activating
factor (PAF) contributed to both phases of neutrophil adhesion.
Anti–intercellular adhesion molecule-1 (ICAM-1) and anti–P-selectin
antibodies (monoclonal antibodies [mAbs]) attenuated phase-1
neutrophil adhesion, consistent with roles for constitutively
expressed ICAM-1 and enhanced surface expression of preformed
P-selectin. Phase-2 neutrophil adhesion was attenuated by an
anti–E-selectin mAb, indicating a dominant role of this adhesion
molecule in the late phase response. Pretreatment with actinomycin D
and cycloheximide or with competing
ds-oligonucleotides containing the
nuclear factor-
B or activator protein-1 cognate DNA
sequences significantly attenuated phase-2 response, suggesting a role
for de novo macromolecule synthesis. Surface expression of ICAM-1,
P-selectin, and E-selectin on HUVECs correlated with the
phase-1 and -2 neutrophil adhesion responses. Collectively, these
findings indicate that A/R elicits a two-phase
neutrophil–endothelial cell adhesion response that
involves transcription-independent and transcription-dependent surface
expression of different endothelial cell adhesion
molecules.
Key Words: nuclear transcription factor • endothelial cell adhesion molecule • leukocyte–endothelial cell adhesion • selectin • intercellular adhesion molecule-1
This article has been cited by other articles:
 |
|
 |
|
  C. I. Jones III, Z. Han, T. Presley, S. Varadharaj, J. L. Zweier, G. Ilangovan, and B. R. Alevriadou Endothelial cell respiration is affected by the oxygen tension during shear exposure: role of mitochondrial peroxynitrite Am J Physiol Cell Physiol, July 1, 2008; 295(1): C180 - C191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Han, Y.-R. Chen, C. I. Jones III, G. Meenakshisundaram, J. L. Zweier, and B. R. Alevriadou Shear-induced reactive nitrogen species inhibit mitochondrial respiratory complex activities in cultured vascular endothelial cells Am J Physiol Cell Physiol, March 1, 2007; 292(3): C1103 - C1112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Aydin, A. J. van Zonneveld, J. W. de Fijter, and T. J. Rabelink New horizons in prevention and treatment of ischaemic injury to kidney transplants Nephrol. Dial. Transplant., February 1, 2007; 22(2): 342 - 346. [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Barshes, S. Wyllie, and J. A. Goss Inflammation-mediated dysfunction and apoptosis in pancreatic islet transplantation: implications for intrahepatic grafts J. Leukoc. Biol., May 1, 2005; 77(5): 587 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Rui, Q. Feng, M. Lei, T. Peng, J. Zhang, M. Xu, E. Dale Abel, A. Xenocostas, and P. R. Kvietys Erythropoietin prevents the acute myocardial inflammatory response induced by ischemia/reperfusion via induction of AP-1 Cardiovasc Res, February 15, 2005; 65(3): 719 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Y. Davani, D. R. Dorscheid, C.-H. Lee, C. van Breemen, and K. R. Walley Novel regulatory mechanism of cardiomyocyte contractility involving ICAM-1 and the cytoskeleton Am J Physiol Heart Circ Physiol, September 1, 2004; 287(3): H1013 - H1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rupin, J. Paysant, P. Sansilvestri-Morel, N. Lembrez, J.-M. Lacoste, A. Cordi, and T. J Verbeuren Role of NADPH oxidase-mediated superoxide production in the regulation of E-selectin expression by endothelial cells subjected to anoxia/reoxygenation Cardiovasc Res, August 1, 2004; 63(2): 323 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Altay, E. R. Gonzales, T. S. Park, and J. M. Gidday Cerebrovascular inflammation after brief episodic hypoxia: modulation by neuronal and endothelial nitric oxide synthase J Appl Physiol, March 1, 2004; 96(3): 1223 - 1230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Rui, G. Cepinskas, Q. Feng, and P. R Kvietys Delayed preconditioning in cardiac myocytes with respect to development of a proinflammatory phenotype: role of SOD and NOS Cardiovasc Res, October 1, 2003; 59(4): 901 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hayashi, K. Fujimoto, K. Urushibata, S.-i. Uchikawa, H. Imamura, and K. Kubo Nocturnal Oxygen Desaturation Correlates With the Severity of Coronary Atherosclerosis in Coronary Artery Disease Chest, September 1, 2003; 124(3): 936 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. S. Kayyali, C. M. Pennella, C. Trujillo, O. Villa, M. Gaestel, and P. M. Hassoun Cytoskeletal Changes in Hypoxic Pulmonary Endothelial Cells Are Dependent on MAPK-activated Protein Kinase MK2 J. Biol. Chem., November 1, 2002; 277(45): 42596 - 42602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. D. Ng, S. S. Deshpande, K. Irani, and B. R. Alevriadou Adhesion of flowing monocytes to hypoxia-reoxygenation-exposed endothelial cells: role of Rac1, ROS, and VCAM-1 Am J Physiol Cell Physiol, July 1, 2002; 283(1): C93 - C102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Lysiak, Q. A. T. Nguyen, and T. T. Turner Peptide and Nonpeptide Reactive Oxygen Scavengers Provide Partial Rescue of the Testis After Torsion J Androl, May 1, 2002; 23(3): 400 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. DYUGOVSKAYA, P. LAVIE, and L. LAVIE Increased Adhesion Molecules Expression and Production of Reactive Oxygen Species in Leukocytes of Sleep Apnea Patients Am. J. Respir. Crit. Care Med., April 1, 2002; 165(7): 934 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Stoica, M. Goddard, and S. R. Large The endothelium in clinical cardiac transplantation Ann. Thorac. Surg., March 1, 2002; 73(3): 1002 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. SINGBARTL, S. B. FORLOW, and K. LEY Platelet, but not endothelial, P-selectin is critical for neutrophil-mediated acute postischemic renal failure FASEB J, November 1, 2001; 15(13): 2337 - 2344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nishijima, J. Kiryu, A. Tsujikawa, M. Honjo, A. Nonaka, K. Yamashiro, H. Tanihara, S. J. Tojo, Y. Ogura, and Y. Honda In Vivo Evaluation of Platelet-Endothelial Interactions after Transient Retinal Ischemia Invest. Ophthalmol. Vis. Sci., August 1, 2001; 42(9): 2102 - 2109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Rui, G. Cepinskas, Q. Feng, Y.-S. Ho, and P. R. Kvietys Cardiac myocytes exposed to anoxia-reoxygenation promote neutrophil transendothelial migration Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H440 - H447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Hu and R. C. Ziegelstein Hypoxia/Reoxygenation Stimulates Intracellular Calcium Oscillations in Human Aortic Endothelial Cells Circulation, November 14, 2000; 102(20): 2541 - 2547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Montrucchio, G. Alloatti, and G. Camussi Role of Platelet-Activating Factor in Cardiovascular Pathophysiology Physiol Rev, October 1, 2000; 80(4): 1669 - 1699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Kim, Y. K. Kim, S. H. Lee, and K. W. Hong Rebamipide Inhibits Neutrophil Adhesion to Hypoxia/Reoxygenation-Stimulated Endothelial Cells via Nuclear Factor-kappa B-Dependent Pathway J. Pharmacol. Exp. Ther., September 1, 2000; 294(3): 864 - 869. [Abstract] [Full Text]
|
|
|
|
|
|
F. M. Akgur, M. F. Brown, G. B. Zibari, J. C. McDonald, C. J. Epstein, C. R. Ross, and D. N. Granger Role of superoxide in hemorrhagic shock-induced P-selectin expression Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H791 - H797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Gopalan, A. R. Burns, S. I. Simon, S. Sparks, L. V. McIntire, and C. W. Smith Preferential sites for stationary adhesion of neutrophils to cytokine-stimulated HUVEC under flow conditions J. Leukoc. Biol., July 1, 2000; 68(1): 47 - 57. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Kokura, R. E. Wolf, T. Yoshikawa, D. N. Granger, and T. Y. Aw Postanoxic T Lymphocyte-Endothelial Cell Interactions Induce Tumor Necrosis Factor-{alpha} Production and Neutrophil Adhesion : Role of Very Late Antigen-4/Vascular Cell Adhesion Molecule-1 Circ. Res., June 23, 2000; 86(12): 1237 - 1244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Russell, C. J. Epstein, M. B. Grisham, J. S. Alexander, K.-Y. Yeh, and D. N. Granger Regulation of E-selectin expression in postischemic intestinal microvasculature Am J Physiol Gastrointest Liver Physiol, June 1, 2000; 278(6): G878 - G885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kokura, R. E. Wolf, T. Yoshikawa, D. N. Granger, and T. Y. Aw T-Lymphocyte-Derived Tumor Necrosis Factor Exacerbates Anoxia-Reoxygenation-Induced Neutrophil-Endothelial Cell Adhesion Circ. Res., February 4, 2000; 86(2): 205 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Y. Aw Molecular and cellular responses to oxidative stress and changes in oxidation-reduction imbalance in the intestine Am. J. Clinical Nutrition, October 1, 1999; 70(4): 557 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Beauchamp, V. Richard, F. Tamion, F. Lallemand, J.-P. Lebreton, H. Vaudry, M. Daveau, and C. Thuillez Protective Effects of Preconditioning in Cultured Rat Endothelial Cells : Effects on Neutrophil Adhesion and Expression of ICAM-1 After Anoxia and Reoxygenation Circulation, August 3, 1999; 100(5): 541 - 546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Willam, R. Schindler, U. Frei, and K.-U. Eckardt Increases in oxygen tension stimulate expression of ICAM-1 and VCAM-1 on human endothelial cells Am J Physiol Heart Circ Physiol, June 1, 1999; 276(6): H2044 - H2052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kokura, R. E. Wolf, T. Yoshikawa, D. N. Granger, and T. Y. Aw Molecular Mechanisms of Neutrophil-Endothelial Cell Adhesion Induced by Redox Imbalance Circ. Res., March 19, 1999; 84(5): 516 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bauer, J. M. Russell, and D. N. Granger Role of endotoxin in intestinal reperfusion-induced expression of E-selectin Am J Physiol Gastrointest Liver Physiol, February 1, 1999; 276(2): G479 - G484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Cepinskas, C. W. Lush, and P. R. Kvietys Anoxia/Reoxygenation-Induced Tolerance With Respect to Polymorphonuclear Leukocyte Adhesion to Cultured Endothelial Cells : A Nuclear Factor-{kappa}B–Mediated Phenomenon Circ. Res., January 22, 1999; 84(1): 103 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. C. Luft, E. Mervaala, D. N. Muller, V. Gross, F. Schmidt, J. K. Park, C. Schmitz, A. Lippoldt, V. Breu, R. Dechend, et al. Hypertension-Induced End-Organ Damage : A New Transgenic Approach to an Old Problem Hypertension, January 1, 1999; 33(1): 212 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Hu, Y. Xia, S. Corda, J. L. Zweier, and R. C. Ziegelstein Hydrogen Peroxide Decreases pHi in Human Aortic Endothelial Cells by Inhibiting Na+/H+ Exchange Circ. Res., September 21, 1998; 83(6): 644 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. H. Koo, K. I. Welsh, J. A. Roake, P. J. Morris, and S. V. Fuggle Ischemia/Reperfusion Injury in Human Kidney Transplantation : An Immunohistochemical Analysis of Changes after Reperfusion Am. J. Pathol., August 1, 1998; 153(2): 557 - 566. [Abstract] [Full Text] [PDF]
|
|