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(Circulation Research. 1996;79:1077-1085.)
© 1996 American Heart Association, Inc.

Articles

Triggered Propagated Contractions in Rat Cardiac Trabeculae

Inhibition by Octanol and Heptanol

Ying Ming Zhang, Masahito Miura, H.E.D.J. ter Keurs

the Department of Medicine, University of Calgary (Canada).

We studied the role of Ca²⁺ diffusion through gap junctions (GJs) in triggering and propagation of damage-induced contractions in cardiac muscle (TPCs) by evaluating effects of the GJ blockers octanol and heptanol (O&H) on TPCs. TPCs were elicited in trabeculae from rat right ventricle superfused with Krebs-Henseleit solution at 20°C and 0.7 to 1.75 mmol/L [Ca²⁺]_o. Force was measured with a silicon strain gauge; sarcomere length, by laser diffraction techniques. O&H (3 to 300 µmol/L) decreased force, propagation velocity, and triggering rate of TPCs in a dose-dependent manner. At 300 µmol/L, O&H decreased TPC force to 21.3% and 25.7%, propagation velocity to 15.4% and 13.0%, and triggering rate to 26.5% and 25.7%. At 300 µmol/L, O&H decreased twitch force to 79.0% and 77.8% and reduced time to 90% relaxation by 10% to 15%. Above 1 mmol/L, O&H abolished twitch force and TPCs. Image analysis of spread of the fluorescence profile of microinjected fura 2 salt revealed an effective diffusion coefficient for fura 2 of 21.0±3.3 µm²/s, which decreased to 12.6±1.5 and 7.07±0.7 µm²/s after 1 and 3 hours of exposure, respectively, to 100 µmol/L octanol, with a time constant of decline of 1.5±0.5 hours. These results are consistent with the hypothesis that propagation of TPCs is due to Ca²⁺-induced Ca²⁺ release mediated by Ca²⁺ diffusion from cell to cell through GJs. Reduction of propagation velocity reduces the number of activated sarcomeres in the TPC, which reduces TPC force. O&H slow triggering of TPCs, presumably by blocking Ca²⁺ diffusion from myocytes within damaged areas to adjacent normal cells.

Key Words: rat cardiac trabecula • triggered propagated contraction • gap junction • octanol • heptanol

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