© 1996 American Heart Association, Inc.
Articles |
Cell Cycle-Dependent Expression of L- and T-Type Ca2+ Currents in Rat Aortic Smooth Muscle Cells in Primary Culture
the Research Institute of Angiocardiology and Cardiovascular Clinic (T.K., Y.H., A.T.) and the Division of Molecular Cardiology (S.K., H.K.), Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Correspondence to Takeshi Kuga, MD, Research Fellow of Japanese Scientific Society, Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.
The expression of L- and T-type Ca2+ channels has been reported to change during various biological events, including cellular differentiation and proliferation. The present study aimed to examine whether or not the expression of L- and T-type Ca2+ channels depends on the cell cycle in rat aortic smooth muscle cells in primary culture. Both the phase of the cell cycle and the functional expression of Ca2+ channels were determined in the same single cell, using an immunocytochemical analysis of cell cycle-specific nuclear antigens and a whole-cell voltage-clamp method, respectively. In the G0 (n=130) and M (n=75) phases, all cells showed only L-type Ca2+ currents. The cells showing a T-type Ca2+ current appeared in the G1 phase (37%, n=85) and increased in the S phase (90%, n=21). For L-type Ca2+ channels, the current density was significantly greater in the G1 phase than in the G0 and M phases. However, either the voltage-dependent properties or the dose-response relationships of Bay K 8644- and second messenger-induced modulations of L-type Ca2+ current did not differ in the four phases of the cell cycle. These findings thus indicate that the expression of L- and T-type Ca2+ channels depends on the cell cycle, whereas the characteristics of L-type Ca2+ channels do not differ between the phases of the cell cycle.
Key Words: Ca2+ channel • ion channel • proliferation • smooth muscle
This article has been cited by other articles:
 |
|
 |
|
  T. Akaike, M.-H. Jin, U. Yokoyama, H. Izumi-Nakaseko, Q. Jiao, S. Iwasaki, M. Iwamoto, S. Nishimaki, M. Sato, S. Yokota, et al. T-type Ca2+ Channels Promote Oxygenation-induced Closure of the Rat Ductus Arteriosus Not Only by Vasoconstriction but Also by Neointima Formation J. Biol. Chem., September 4, 2009; 284(36): 24025 - 24034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Berra-Romani, A. Mazzocco-Spezzia, M. V. Pulina, and V. A. Golovina Ca2+ handling is altered when arterial myocytes progress from a contractile to a proliferative phenotype in culture Am J Physiol Cell Physiol, September 1, 2008; 295(3): C779 - C790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Ng, B. D. Kyle, A. R. Lennox, X.-M. Shen, W. J. Hatton, and J. R. Hume Cell culture alters Ca2+ entry pathways activated by store-depletion or hypoxia in canine pulmonary arterial smooth muscle cells Am J Physiol Cell Physiol, January 1, 2008; 294(1): C313 - C323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lipskaia, C. Pinet, Y. Fromes, S. Hatem, I. Cantaloube, A. Coulombe, and A.-M. Lompre Mutation of {delta}-Sarcoglycan Is Associated with Ca2+-Dependent Vascular Remodeling in the Syrian Hamster Am. J. Pathol., July 1, 2007; 171(1): 162 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Afroze, A. M. Sadi, M. A. Momen, S. Gu, S. Heximer, and M. Husain c-Myb-Dependent Inositol 1,4,5-Trisphosphate Receptor Type-1 Expression in Vascular Smooth Muscle Cells Arterioscler Thromb Vasc Biol, June 1, 2007; 27(6): 1305 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Nikitina, Z.-D. Zhang, A. Kawashima, B. S. Jahromi, V. A. Bouryi, M. Takahashi, A. Xie, and R. L. Macdonald Voltage-dependent calcium channels of dog basilar artery J. Physiol., April 15, 2007; 580(2): 523 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tiwari, Y. Zhang, J. Heller, D. R. Abernethy, and N. M. Soldatov Atherosclerosis-related molecular alteration of the human CaV1.2 calcium channel {alpha}1C subunit PNAS, November 7, 2006; 103(45): 17024 - 17029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Fukunaga, K. Hirano, M. Hirano, N. Niiro, J. Nishimura, Y. Maehara, and H. Kanaide Upregulation of proteinase-activated receptors and hypercontractile responses precede development of arterial lesions after balloon injury Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2388 - H2395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Yokoyama, S. Minamisawa, S. Adachi-Akahane, T. Akaike, I. Naguro, K. Funakoshi, M. Iwamoto, M. Nakagome, N. Uemura, H. Hori, et al. Multiple transcripts of Ca2+ channel {alpha}1-subunits and a novel spliced variant of the {alpha}1C-subunit in rat ductus arteriosus Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1660 - H1670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Wilkerson, T. J. Heppner, A. D. Bonev, and M. T. Nelson Inositol trisphosphate receptor calcium release is required for cerebral artery smooth muscle cell proliferation Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H240 - H247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Miguel-Velado, A. Moreno-Dominguez, O. Colinas, P. Cidad, M. Heras, M. T. Perez-Garcia, and J. R. Lopez-Lopez Contribution of Kv Channels to Phenotypic Remodeling of Human Uterine Artery Smooth Muscle Cells Circ. Res., December 9, 2005; 97(12): 1280 - 1287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Rodman, K. Reese, J. Harral, B. Fouty, S. Wu, J. West, M. Hoedt-Miller, Y. Tada, K.-X. Li, C. Cool, et al. Low-Voltage-Activated (T-Type) Calcium Channels Control Proliferation of Human Pulmonary Artery Myocytes Circ. Res., April 29, 2005; 96(8): 864 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Larsen, C.-C. Chen, and P. M. Best Disruption of growth hormone secretion alters Ca2+ current density and expression of Ca2+ channel and insulin-like growth factor genes in rat atria Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H829 - H838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. I. Brueggemann, B. L. Martin, J. Barakat, K. L. Byron, and L. L. Cribbs Low voltage-activated calcium channels in vascular smooth muscle: T-type channels and AVP-stimulated calcium spiking Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H923 - H935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yu, I. Fantozzi, C. V. Remillard, J. W. Landsberg, N. Kunichika, O. Platoshyn, D. D. Tigno, P. A. Thistlethwaite, L. J. Rubin, and J. X.-J. Yuan Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension PNAS, September 21, 2004; 101(38): 13861 - 13866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cataldi, A. Gaudino, V. Lariccia, M. Russo, S. Amoroso, G. di Renzo, and L. Annunziato Imatinib-Mesylate Blocks Recombinant T-Type Calcium Channels Expressed in Human Embryonic Kidney-293 Cells by a Protein Tyrosine Kinase-Independent Mechanism J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 208 - 215. [Abstract] [Full Text]
|
|
|
|
|
|
L. Ferron, V. Capuano, Y. Ruchon, E. Deroubaix, A. Coulombe, and J.-F. Renaud Angiotensin II Signaling Pathways Mediate Expression of Cardiac T-Type Calcium Channels Circ. Res., December 12, 2003; 93(12): 1241 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-C. Chen, K. G. Lamping, D. W. Nuno, R. Barresi, S. J. Prouty, J. L. Lavoie, L. L. Cribbs, S. K. England, C. D. Sigmund, R. M. Weiss, et al. Abnormal Coronary Function in Mice Deficient in {alpha}1H T-type Ca2+ Channels Science, November 21, 2003; 302(5649): 1416 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Del Toro, K. L. Levitsky, J. Lopez-Barneo, and M. D. Chiara Induction of T-type Calcium Channel Gene Expression by Chronic Hypoxia J. Biol. Chem., June 13, 2003; 278(25): 22316 - 22324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lipskaia, M.-L. Pourci, C. Delomenie, L. Combettes, D. Goudouneche, J.-L. Paul, T. Capiod, and A.-M. Lompre Phosphatidylinositol 3-Kinase and Calcium-Activated Transcription Pathways Are Required for VLDL-Induced Smooth Muscle Cell Proliferation Circ. Res., May 30, 2003; 92(10): 1115 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Perez-Reyes Molecular Physiology of Low-Voltage-Activated T-type Calcium Channels Physiol Rev, January 1, 2003; 83(1): 117 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Nakao, S. Kobayashi, K. Mogami, Y. Mizukami, S. Shirao, S. Miwa, N. Todoroki-Ikeda, M. Ito, and M. Matsuzaki Involvement of Src Family Protein Tyrosine Kinases in Ca2+ Sensitization of Coronary Artery Contraction Mediated by a Sphingosylphosphorylcholine-Rho-Kinase Pathway Circ. Res., November 15, 2002; 91(10): 953 - 960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Bertolesi, C. Shi, L. Elbaum, C. Jollimore, G. Rozenberg, S. Barnes, and M. E. M. Kelly The Ca2+ Channel Antagonists Mibefradil and Pimozide Inhibit Cell Growth via Different Cytotoxic Mechanisms Mol. Pharmacol., August 1, 2002; 62(2): 210 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hirooka, G. E. Bertolesi, M. E. M. Kelly, E. M. Denovan-Wright, X. Sun, J. Hamid, G. W. Zamponi, A. E. Juhasz, L. W. Haynes, and S. Barnes T-Type Calcium Channel alpha 1G and alpha 1H Subunits in Human Retinoblastoma Cells and Their Loss After Differentiation J Neurophysiol, July 1, 2002; 88(1): 196 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sweeney, Y. Yu, O. Platoshyn, S. Zhang, S. S. McDaniel, and J. X.-J. Yuan Inhibition of endogenous TRP1 decreases capacitative Ca2+ entry and attenuates pulmonary artery smooth muscle cell proliferation Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L144 - L155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mariot, K. Vanoverberghe, N. Lalevee, M. F. Rossier, and N. Prevarskaya Overexpression of an alpha 1H (Cav3.2) T-type Calcium Channel during Neuroendocrine Differentiation of Human Prostate Cancer Cells J. Biol. Chem., March 22, 2002; 277(13): 10824 - 10833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Koh, K. Monaghan, S. Ro, H. S Mason, J. L Kenyon, and K. M Sanders Novel voltage-dependent non-selective cation conductance in murine colonic myocytes J. Physiol., June 1, 2001; 533(2): 341 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-F. Quignard, M.-C. Harricane, C. Menard, P. Lory, J. Nargeot, L. Capron, D. Mornet, and S. Richard Transient down-regulation of L-type Ca2+ channel and dystrophin expression after balloon injury in rat aortic cells Cardiovasc Res, January 1, 2001; 49(1): 177 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pignier and D. Potreau Characterization of nifedipine-resistant calcium current in neonatal rat ventricular cardiomyocytes Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2259 - H2268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Maric, I. Maric, and J. L. Barker Developmental Changes in Cell Calcium Homeostasis during Neurogenesis of the Embryonic Rat Cerebral Cortex Cereb Cortex, June 1, 2000; 10(6): 561 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Vallot, L. Combettes, P. Jourdon, J. Inamo, I. Marty, M. Claret, and A.-M. Lompre Intracellular Ca2+ Handling in Vascular Smooth Muscle Cells Is Affected by Proliferation Arterioscler Thromb Vasc Biol, May 1, 2000; 20(5): 1225 - 1235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nargeot A Tale of Two (Calcium) Channels Circ. Res., March 31, 2000; 86(6): 613 - 615. [Full Text] [PDF]
|
|
|
|
|
|
A. Monteil, J. Chemin, E. Bourinet, G. Mennessier, P. Lory, and J. Nargeot Molecular and Functional Properties of the Human alpha 1G Subunit That Forms T-type Calcium Channels J. Biol. Chem., February 25, 2000; 275(9): 6090 - 6100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, A. Bhattacharjee, Z. Zuo, F. Hu, R. E. Honkanen, P.-O. Berggren, and M. Li A Low Voltage-Activated Ca2+ Current Mediates Cytokine-Induced Pancreatic {beta}-Cell Death Endocrinology, March 1, 1999; 140(3): 1200 - 1204. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. A. Pardo, A. Bruggemann, J. Camacho, and W. Stuhmer Cell Cycle-related Changes in the Conducting Properties of r-eag K+ Channels J. Cell Biol., November 2, 1998; 143(3): 767 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gomez and K. Sward Long-term regulation of contractility and calcium current in smooth muscle Am J Physiol Cell Physiol, November 1, 1997; 273(5): C1714 - C1720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. d. F. Cortes, V. S. Lemos, and J.-C. Stoclet Alterations in Calcium Stores in Aortic Myocytes From Spontaneously Hypertensive Rats Hypertension, June 1, 1997; 29(6): 1322 - 1328. [Abstract] [Full Text]
|
|