This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nielsen, L. B. Articles by Nordestgaard, B. G. Search for Related Content PubMed PubMed Citation Articles by Nielsen, L. B. Articles by Nordestgaard, B. G. Pubmed/NCBI databases Substance via MeSH

(Circulation Research. 1996;78:615-626.)
© 1996 American Heart Association, Inc.

Articles

Specific Accumulation of Lipoprotein(a) in Balloon-Injured Rabbit Aorta In Vivo

Lars B. Nielsen, Steen Stender, Knud Kjeldsen, Børge G. Nordestgaard

From the Department of Clinical Biochemistry, Rigshospitalet (L.B.N., K.K.), the Department of Clinical Chemistry, Køge Hospital (S.S.), and the Department of Clinical Biochemistry, Herlev Hospital (B.G.N.), University of Copenhagen, Denmark.

Abstract To explore whether lipoprotein(a), Lp(a), may accumulate preferentially to LDL in the arterial wall at sites of injury, cholesterol-fed rabbits were injected intravenously with radiolabeled Lp(a) and/or LDL 3.1±0.1 days (mean±SEM, n=30) after a balloon injury of the thoracic aorta. After 5 to 10 minutes' exposure to labeled lipoproteins, more labeled LDL than labeled Lp(a) was recovered in the intima–inner media of the balloon-injured segment (n=9; paired t test, P<.0001); however, the amount of tightly bound labeled lipoprotein was similar for the two lipoprotein fractions. In the second set of experiments, ¹³¹I-Lp(a) (or ¹³¹I-LDL) was injected 26 hours before and ¹²⁵I-Lp(a) (or ¹²⁵I-LDL) 3 hours before the aorta was removed. Permeability and fractional loss of labeled Lp(a) (n=8) versus LDL (n=7) in the balloon-injured aortic intima–inner media were: permeability, 0.46±0.10 µL/cm² per hour versus 1.41±0.32 µL/cm² per hour (nonpaired t test, P<.0001); and fractional loss, 0.12±0.02 h^-1 versus 0.44±0.05 h^-1 (nonpaired t test, P=.0001), respectively. Finally, after 23 hours' exposure to labeled lipoproteins, the total accumulation and the amount of tightly bound labeled Lp(a) in the balloon-injured intima–inner media were, respectively, 174% (n=6; ANOVA, P=.03) and 256% (ANOVA, P=.005) of the values for labeled LDL. For labeled Lp(a) in the balloon-injured compared with the normal aortic intima–inner media, the recovery after 5 to 10 minutes, the permeability, and the accumulation after 23 hours were all increased, whereas the fractional loss was unchanged. These data suggest that the accumulation of Lp(a) is much larger in injured vessels than in normal vessels. Moreover, the data support the idea of a specific accumulation of Lp(a) compared with LDL in injured vessels.

Key Words: angioplasty • atherosclerosis • lipoprotein(a) • low-density lipoprotein • restenosis

This article has been cited by other articles:

				P. R. Kamstrup, A. Tybjaerg-Hansen, R. Steffensen, and B. G. Nordestgaard Genetically Elevated Lipoprotein(a) and Increased Risk of Myocardial Infarction JAMA, June 10, 2009; 301(22): 2331 - 2339. [Abstract] [Full Text] [PDF]

				P. R. Kamstrup, M. Benn, A. Tybjaerg-Hansen, and B. G. Nordestgaard Extreme Lipoprotein(a) Levels and Risk of Myocardial Infarction in the General Population: The Copenhagen City Heart Study Circulation, January 15, 2008; 117(2): 176 - 184. [Abstract] [Full Text] [PDF]

				T. Bjornheden, G. Bondjers, and O. Wiklund Direct Assessment of Lipoprotein Outflow From In Vivo–Labeled Arterial Tissue as Determined in an In Vitro Perfusion System Arterioscler. Thromb. Vasc. Biol., December 1, 1998; 18(12): 1927 - 1933. [Abstract] [Full Text] [PDF]

				L. B. Nielsen, K. Juul, and B. G. Nordestgaard Increased Degradation of Lipoprotein(a) in Atherosclerotic Compared With Nonlesioned Aortic Intima–Inner Media of Rabbits : In Vivo Evidence That Lipoprotein(a) May Contribute to Foam Cell Formation Arterioscler. Thromb. Vasc. Biol., April 1, 1998; 18(4): 641 - 649. [Abstract] [Full Text] [PDF]

				L. B. Nielsen, M. L.M. Gronholdt, T. V. Schroeder, S. Stender, and B. G. Nordestgaard In Vivo Transfer of Lipoprotein(a) Into Human Atherosclerotic Carotid Arterial Intima Arterioscler. Thromb. Vasc. Biol., May 1, 1997; 17(5): 905 - 911. [Abstract] [Full Text]

				R. M. Lawn, A. D. Pearle, L. L. Kunz, E. M. Rubin, J. Reckless, J. C. Metcalfe, and D. J. Grainger Feedback Mechanism of Focal Vascular Lesion Formation in Transgenic Apolipoprotein(a) Mice J. Biol. Chem., December 6, 1996; 271(49): 31367 - 31371. [Abstract] [Full Text] [PDF]

				R. Klose, F. Fresser, S. Kochl, W. Parson, A. Kapetanopoulos, J. Fruchart-Najib, G. Baier, and G. Utermann Mapping of a Minimal Apolipoprotein(a) Interaction Motif Conserved in Fibrin(ogen) beta - and gamma -Chains J. Biol. Chem., December 1, 2000; 275(49): 38206 - 38212. [Abstract] [Full Text] [PDF]