© 1996 American Heart Association, Inc.
Articles |
Regulated Expression of the Ets-1 Transcription Factor in Vascular Smooth Muscle Cells In Vivo and In Vitro
From the Division of Cardiology (A.H.-N., B.C., J.-W.W., S.N., B.S., J.S.F., J.A.F.), Cedars-Sinai Medical Center, UCLA Medical School, Los Angeles, Calif, and the Department of Cell and Molecular Biology (A.H.-N., C.L.), Medical Nobel Institute, Laboratory of Medical Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Correspondence to Dr James A. Fagin, Division of Endocrinology and Metabolism, University of Cincinnati, College of Medicine, PO Box 670547, Cincinnati, OH 45267.
Abstract Ets-1 regulates the transcription of several genes encoding extracellular matrix proteins (ie, osteopontin and tenascin) as well as enzymes involved in degradation and remodeling of the extracellular matrix (ie, stromelysin and urokinase plasminogen activator). In the present study, we investigated the regulation of c-ets-1 in cultured rat vascular smooth muscle cells as well as in the arterial wall after balloon injury in vivo. Serum-starved smooth muscle cells exposed to serum for various time points express a major c-ets-1 mRNA transcript of 5.3 kb and minor bands of 4.0 and 2.5 kb with a peak at 2 hours after stimulation. These effects were concentration dependent. Western blotting revealed an increase in 55- and 40-kD immunoreactive ets-1 proteins in cells treated with serum for 2 hours, and binding to an oligonucleotide containing the ets-1 consensus cis-acting motif was demonstrated by electrophoretic mobility shift assay. Ets-1 mRNA abundance was induced with a peak at 2 hours after stimulation with platelet-derived growth factor-BB and with angiotensin II. There was a distinct increase of ets-1 immunoreactivity in the inner layer of the media 2 hours after balloon catheter injury of rat arteries, which declined after 6 hours and returned to the basal level 1 day after vessel wall damage. Arterial c-ets-1 mRNA content was induced with an identical time course. These findings suggest that c-ets-1 may be of importance in the mitogenic signaling pathway of smooth muscle cells grown in culture. In addition, ets-1 may play a role in the activation of smooth muscle cells in vivo after mechanical injury of the vessel wall. Because the ets-1 transcription factor activates the gene expression of a number of mRNA species involved in matrix deposition and degradation, these data are compatible with a role for ets-1 in vascular remodeling and/or cell migration.
Key Words: ets-1 • smooth muscle cells • matrix metalloproteinases
This article has been cited by other articles:
 |
|
 |
|
  P. Kumar and K. N. Pandey Cooperative Activation of Npr1 Gene Transcription and Expression by Interaction of Ets-1 and p300 Hypertension, July 1, 2009; 54(1): 172 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Perez Sastre, S. Grossmann, H. P. Reusch, and M. Schaefer Requirement of an Intermediate Gene Expression for Biphasic ERK1/2 Activation in Thrombin-stimulated Vascular Smooth Muscle Cells J. Biol. Chem., September 19, 2008; 283(38): 25871 - 25878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. Malabanan, P. Kanellakis, A. Bobik, and L. M. Khachigian Activation Transcription Factor-4 Induced by Fibroblast Growth Factor-2 Regulates Vascular Endothelial Growth Factor-A Transcription in Vascular Smooth Muscle Cells and Mediates Intimal Thickening in Rat Arteries Following Balloon Injury Circ. Res., August 15, 2008; 103(4): 378 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Pearse, R.-X. Tian, J. Nigro, J. B. Iorgulescu, L. Puzis, and E. A. Jaimes Angiotensin II increases the expression of the transcription factor ETS-1 in mesangial cells Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1094 - F1100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ni, Y. Zhan, H. He, E. Maynard, J. A. Balschi, and P. Oettgen Ets-1 Is a Critical Transcriptional Regulator of Reactive Oxygen Species and p47phox Gene Expression in Response to Angiotensin II Circ. Res., November 9, 2007; 101(10): 985 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Oettgen Regulation of Vascular Inflammation and Remodeling by ETS Factors Circ. Res., November 24, 2006; 99(11): 1159 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ogawa, T. Nomiyama, T. Nakamachi, E. B. Heywood, J. F. Stone, J. P. Berger, R. E. Law, and D. Bruemmer Activation of Peroxisome Proliferator-Activated Receptor {gamma} Suppresses Telomerase Activity in Vascular Smooth Muscle Cells Circ. Res., April 14, 2006; 98(7): e50 - e59. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Bonello, Y. V. Bobryshev, and L. M. Khachigian Peroxide-Inducible Ets-1 Mediates Platelet-Derived Growth Factor Receptor-{alpha} Gene Transcription in Vascular Smooth Muscle Cells Am. J. Pathol., October 1, 2005; 167(4): 1149 - 1159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tanaka, Y. Terada, T. Kobayashi, T. Okado, S. Inoshita, M. Kuwahara, A. Seth, Y. Sato, and S. Sasaki Expression and Function of Ets-1 during Experimental Acute Renal Failure in Rats J. Am. Soc. Nephrol., December 1, 2004; 15(12): 3083 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. Santiago and L. M. Khachigian Ets-1 Stimulates Platelet-Derived Growth Factor A-Chain Gene Transcription and Vascular Smooth Muscle Cell Growth via Cooperative Interactions With Sp1 Circ. Res., September 3, 2004; 95(5): 479 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Dandre and G. K. Owens Platelet-derived growth factor-BB and Ets-1 transcription factor negatively regulate transcription of multiple smooth muscle cell differentiation marker genes Am J Physiol Heart Circ Physiol, June 1, 2004; 286(6): H2042 - H2051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Watanabe, H. Takagi, K. Suzuma, I. Suzuma, H. Oh, H. Ohashi, S. Kemmochi, A. Uemura, T. Ojima, E. Suganami, et al. Transcription Factor Ets-1 Mediates Ischemia- and Vascular Endothelial Growth Factor-Dependent Retinal Neovascularization Am. J. Pathol., May 1, 2004; 164(5): 1827 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-A. Renault, S. Jalvy, I. Belloc, S. Pasquet, S. Sena, M. Olive, C. Desgranges, and A.-P. Gadeau AP-1 Is Involved in UTP-Induced Osteopontin Expression in Arterial Smooth Muscle Cells Circ. Res., October 3, 2003; 93(7): 674 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reisdorff, A. En-Nia, I. Stefanidis, J. Floege, D. H. Lovett, and P. R. Mertens Transcription Factor Ets-1 Regulates Gelatinase A Gene Expression in Mesangial Cells J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1568 - 1578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-D. Orzechowski, A. Gunther, S. Menzel, A. Zimmermann, H. Funke-Kaiser, R. Real, T. Subkowski, F. S. Zollmann, and M. Paul Transcriptional Mechanism of Protein Kinase C-Induced Isoform-Specific Expression of the Gene for Endothelin-Converting Enzyme-1in Human Endothelial Cells Mol. Pharmacol., December 1, 2001; 60(6): 1332 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. NAITO, M. S. RAZZAQUE, A. NAZNEEN, D. LIU, H. NIHEI, T. KOJI, and T. TAGUCHI Renal Expression of the Ets-1 Proto-oncogene during Progression of Rat Crescentic Glomerulonephritis J. Am. Soc. Nephrol., December 1, 2000; 11(12): 2243 - 2255. [Abstract] [Full Text]
|
|
|
|
|
|
T. Knittel, D. Kobold, J. Dudas, B. Saile, and G. Ramadori Role of the Ets-1 Transcription Factor during Activation of Rat Hepatic Stellate Cells in Culture Am. J. Pathol., December 1, 1999; 155(6): 1841 - 1848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kawai-Kowase, M. Kurabayashi, Y. Hoshino, Y. Ohyama, and R. Nagai Transcriptional Activation of the Zinc Finger Transcription Factor BTEB2 Gene by Egr-1 Through Mitogen-Activated Protein Kinase Pathways in Vascular Smooth Muscle Cells Circ. Res., October 29, 1999; 85(9): 787 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Day, L. A. Rafty, C. N. Chesterman, and L. M. Khachigian Angiotensin II (ATII)-inducible Platelet-derived Growth Factor A-chain Gene Expression Is p42/44 Extracellular Signal-regulated Kinase-1/2 and Egr-1-dependent and Mediated via the ATII Type 1 but Not Type 2 Receptor. INDUCTION BY ATII ANTAGONIZED BY NITRIC OXIDE J. Biol. Chem., August 20, 1999; 274(34): 23726 - 23733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Watanabe, M. Kurabayashi, Y. Shimomura, K. Kawai-Kowase, Y.-i. Hoshino, I. Manabe, M. Watanabe, M. Aikawa, M. Kuro-o, T. Suzuki, et al. BTEB2, a Kruppel-Like Transcription Factor, Regulates Expression of the SMemb/Nonmuscle Myosin Heavy Chain B (SMemb/NMHC-B) Gene Circ. Res., July 23, 1999; 85(2): 182 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Khachigian, F. S. Santiago, L. A. Rafty, O. L.-W. Chan, G. J. Delbridge, A. Bobik, T. Collins, and A. C. Johnson GC Factor 2 Represses Platelet-Derived Growth Factor A-Chain Gene Transcription and Is Itself Induced by Arterial Injury Circ. Res., June 11, 1999; 84(11): 1258 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Naito, S. Shimizu, S. Maeda, J. Wang, R. Paul, and J. A. Fagin Ets-1 is an early response gene activated by ET-1 and PDGF-BB in vascular smooth muscle cells Am J Physiol Cell Physiol, February 1, 1998; 274(2): C472 - C480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hultgardh-Nilsson, C. Lovdahl, K. Blomgren, Bengt Kallin, and J. Thyberg Expression of phenotype- and proliferation-related genes in rat aortic smooth muscle cells in primary culture Cardiovasc Res, May 1, 1997; 34(2): 418 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jovinge, A. Hultgardh-Nilsson, J. Regnstrom, and J. Nilsson Tumor Necrosis Factor-{alpha} Activates Smooth Muscle Cell Migration in Culture and Is Expressed in the Balloon-Injured Rat Aorta Arterioscler. Thromb. Vasc. Biol., March 1, 1997; 17(3): 490 - 497. [Abstract] [Full Text]
|
|