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(Circulation Research. 1996;78:362-370.)
© 1996 American Heart Association, Inc.

Articles

Generation of New Intercellular Junctions Between Cardiocytes

A Possible Mechanism Compensating for Mechanical Overload in the Hypertrophied Human Adult Myocardium

Shoji Yamamoto, Thomas N. James, Ken-ichi Sawada, Makoto Okabe, Keishiro Kawamura

From the World Health Organization Cardiovascular Center and the Department of Medicine and Department of Pathology at the University of Texas Medical Branch (S.Y., T.N.J.), Galveston, and the Osaka (Japan) Medical College (K.S., M.O., K.K.).

Correspondence to Thomas N. James, MD, Office of the President, University of Texas Medical Branch, Galveston, TX 77555-0129.

Abstract Intercellular dehiscence is a common cardiocytic response to pathological conditions. However, little consideration has been given to the possibility of new intercellular junctions developing between cardiocytes within developed myocardium. To examine this possibility as it may relate to useful compensation for hemodynamic overloads, changes in cardiocytic connection were evaluated by scanning electron microscopy in hypertrophied myocardium of adult human hearts. Transmural myocardium of left ventricle was obtained at autopsy from five hearts with concentric hypertrophy, five hearts with eccentric hypertrophy, and five control hearts (noncardiac death). After formalin fixation, the number of cardiocytes connected to an individual cardiocyte was counted in tissues from the middle portion of the transmural samples by scanning electron microscopy. Cardiocytic diameter and connective tissue volume fraction were measured on the transmural sections by light microscopy. In concentrically hypertrophied hearts presenting both increased cardiocytic diameter and connective tissue volume fraction, the number of other cardiocytes connected to an individual cardiocyte (4.60±0.10 [mean±SE]) was significantly increased (P<.05) compared with control hearts (4.19±0.12) or eccentrically hypertrophied hearts (4.11±0.10). The increase in junctions per cardiocyte in concentrically hypertrophied hearts suggests that new connections had been generated. More junctions developing during hypertrophy could add another structural advantage to those of cardiocytic hypertrophy and connective tissue proliferation as compensatory adjustments to hemodynamic overload in concentrically hypertrophied hearts.

Key Words: intercalated disk • cell adhesion • scanning electron microscopy • concentric hypertrophy • eccentric hypertrophy

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