© 1996 American Heart Association, Inc.
Articles |
Regulation of Very-Low-Density Lipoprotein Receptor in Hypertrophic Rat Heart
From the Department of Medicine and Clinical Science (H.M., H.J., N.M., O.N., Y.O., M.M., Y.Y., K.N.), Kyoto University Graduate School of Medicine, Kyoto, and the Tohoku University Gene Research Center (T.Y.), Sendai, Japan.
Correspondence to Hisato Jingami, MD, PhD, Department of Molecular Biology, Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565, Japan.
Abstract To elucidate the regulation of very-low-density lipoprotein (VLDL) receptor, we have studied its gene expression in the heart of spontaneously hypertensive rats–stroke prone (SHR-SP, an animal model for hypertension-induced cardiac hypertrophy) compared with Wistar-Kyoto rats. RNase protection assay showed that ventricular VLDL receptor mRNA falls to 41% of normal levels at 4 weeks, when hypertension is not yet fully developed, and drops further to 14% at 13 weeks, when cardiac hypertrophy is established. Lipoprotein lipase mRNA decreases in parallel with VLDL receptor mRNA. In cultured neonatal rat ventricular cardiomyocytes, VLDL receptor mRNA decreases in parallel with the process of cardiocyte hypertrophy during the 24 hours after treatment with 10-8 mol/L endothelin-1, falling to 40% of the initial value. These results demonstrate that there is downregulation of VLDL receptor gene expression in cardiac hypertrophy both in vivo and in vitro and suggest that the regulation of the VLDL receptor is possibly linked with the switch in energy substrate from lipid to glucose known to occur in cardiac hypertrophy.
Key Words: cardiomyocyte culture • very-low-density lipoprotein receptor • spontaneously hypertensive rats–stroke prone • cardiac hypertrophy
This article has been cited by other articles:
 |
|
 |
|
  D. Han, N. H. Haunerland, and T. D. Williams Variation in yolk precursor receptor mRNA expression is a key determinant of reproductive phenotype in the zebra finch (Taeniopygia guttata) J. Exp. Biol., May 1, 2009; 212(9): 1277 - 1283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Jia, M. Takahashi, H. Morimoto, S. Takahashi, A. Izawa, H. Ise, T. Iwasaki, H. Hattori, K.-J. Wu, and U. Ikeda Changes in cardiac lipid metabolism during sepsis: The essential role of very low-density lipoprotein receptors Cardiovasc Res, February 1, 2006; 69(2): 545 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shimokawa, K.-i. Hirata, T. Ishida, Y. Kojima, N. Inoue, T. Quertermous, and M. Yokoyama Increased expression of endothelial lipase in rat models of hypertension Cardiovasc Res, June 1, 2005; 66(3): 594 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-G. Niu, D. Hauton, and R. D. Evans Utilization of triacylglycerol-rich lipoproteins by the working rat heart: routes of uptake and metabolic fates J. Physiol., July 1, 2004; 558(1): 225 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L.J.L Thijssen, H. M.W van der Velden, E. P van Ankeren, J. Ausma, M. A Allessie, M. Borgers, G. J.J.M van Eys, and H. J Jongsma Analysis of altered gene expression during sustained atrial fibrillation in the goat Cardiovasc Res, May 1, 2002; 54(2): 427 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zaugg, M. C. Schaub, T. Pasch, and D. R. Spahn Modulation of {beta}-adrenergic receptor subtype activities in perioperative medicine: mechanisms and sites of action Br. J. Anaesth., January 1, 2002; 88(1): 101 - 123. [Abstract] [Full Text] [PDF]
|
|