Donate Help Contact The AHA Sign In Home
American Heart Association
Circulation Research
Search: search_blue_button Advanced Search
Circulation Research. 1995;77:943-949

This Article
Right arrow Full Text
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Patel, J. R.
Right arrow Articles by Moss, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Patel, J. R.
Right arrow Articles by Moss, R. L.
(Circulation Research. 1995;77:943.)
© 1995 American Heart Association, Inc.


Articles

Cardiac Sarcoplasmic Reticulum Phosphorylation Increases Ca2+ Release Induced by Flash Photolysis of Nitr-5

Jitandrakumar R. Patel, Roberto Coronado, Richard L. Moss

From the Department of Physiology, University of Wisconsin Medical School, Madison.

Correspondence to Dr Richard L. Moss, Department of Physiology, University of Wisconsin Medical School, 1300 University Ave, Madison, WI 53706.

Abstract Effects on Ca2+-induced Ca2+ release due to phosphorylation of sarcoplasmic reticulum (SR) proteins were investigated in isoproterenol-treated saponin-permeabilized trabeculae from rat ventricles. In these experiments, Ca2+ release from the SR was induced by a rapid change in concentration of free Ca2+ (ie, trigger Ca2+) achieved by flash photolysis of nitr-5, and the amount of Ca2+ released was assessed by measuring isometric tension. Ca2+ uptake by the SR was more rapid, and the amount of Ca2+ released by a given concentration of trigger Ca2+ was greater in isoproterenol-treated trabeculae compared with control trabeculae. However, under the same conditions of Ca2+ loading, the amplitudes of caffeine-elicited tension transients in control trabeculae were similar to those in isoproterenol-treated trabeculae, suggesting that the Ca2+ available for release was similar in the two cases. Control experiments showed that there were no significant differences in Ca2+ sensitivity of tension between isoproterenol-treated and control trabeculae. Also, application of alkaline phosphatase to trabeculae that had previously been treated with isoproterenol returned SR Ca2+ release to control levels. We conclude that the greater release of Ca2+ in isoproterenol-treated trabeculae in response to a given concentration of trigger Ca2+ is due to phosphorylation of SR proteins, most likely the Ca2+ release channel.


Key Words: ventricular trabeculae • sarcoplasmic reticulum • Ca2+-induced Ca2+ release • flash photolysis • phosphorylation




This article has been cited by other articles:


Home page
Physiol. Rev.Home page
E. Carmeliet
Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias
Physiol Rev, July 1, 1999; 79(3): 917 - 1017.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
Y.-K. Ju and D. G Allen
How does {beta}-adrenergic stimulation increase the heart rate? The role of intracellular Ca2+ release in amphibian pacemaker cells
J. Physiol., May 1, 1999; 516(3): 793 - 804.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
D.A Eisner, A.W Trafford, M.E Dnaz, C.L Overend, and S.C O'Neill
The control of Ca release from the cardiac sarcoplasmic reticulum: regulation versus autoregulation
Cardiovasc Res, June 1, 1998; 38(3): 589 - 604.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
Y.G. Wang, J. Huser, L.A. Blatter, and S.L. Lipsius
Withdrawal of Acetylcholine Elicits Ca2+-Induced Delayed Afterdepolarizations in Cat Atrial Myocytes
Circulation, August 19, 1997; 96(4): 1275 - 1281.
[Abstract] [Full Text]


Home page
Pharmacol. Rev.Home page
R. Zucchi and S. Ronca-Testoni
The Sarcoplasmic Reticulum Ca2+ Channel/Ryanodine Receptor: Modulation by Endogenous Effectors, Drugs and Disease States
Pharmacol. Rev., March 1, 1997; 49(1): 1 - 52.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
L.-S. Song, S.-Q. Wang, R.-P. Xiao, H. Spurgeon, E. G. Lakatta, and H. Cheng
{beta}-Adrenergic Stimulation Synchronizes Intracellular Ca2+ Release During Excitation-Contraction Coupling in Cardiac Myocytes
Circ. Res., April 27, 2001; 88(8): 794 - 801.
[Abstract] [Full Text] [PDF]