© 1995 American Heart Association, Inc.
Articles |
Low Concentration of Oxidized Low-Density Lipoprotein and Lysophosphatidylcholine Upregulate Constitutive Nitric Oxide Synthase mRNA Expression in Bovine Aortic Endothelial Cells
From The First Department of Internal Medicine, Kobe (Japan) University School of Medicine.
Correspondence to Ken-ichi Hirata, MD, The First Department of Internal Medicine, Kobe University School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650, Japan.
Abstract Endothelium-dependent relaxation is markedly reduced in atherosclerotic arteries. Recently, the endothelium-dependent relaxing factor has been identified as nitric oxide (NO). We used RNase protection assay and immunoblotting to elucidate the effect of atherogenic lipoprotein on the expression of constitutive NO synthase (cNOS) mRNA and protein levels in bovine aortic endothelial cells. Twenty-four-hour exposure to a low concentration of oxidized low-density lipoprotein (10 µg protein/mL) upregulated cNOS mRNA levels (2.4±0.4-fold, P<.01). However, native low-density lipoprotein and high-density lipoprotein did not have any effect on cNOS mRNA levels. Furthermore, 5 µg/mL of lysophosphatidylcholine (LPC) also upregulated cNOS mRNA levels (2.6±0.5-fold, P<.01) at 8 hours. This action of LPC was abolished with cycloheximide but not with staurosporine. We concluded that atherogenic lipoproteins upregulate cNOS mRNA and protein levels in bovine aortic endothelial cells. This observation supports the hypothesis that an impairment of endothelium-dependent vasodilatation in atherosclerotic vessels may not be due to a decrease in cNOS expression. Moreover, the LPC action on cNOS mRNA levels requires new protein synthesis.
Key Words: oxidized low-density lipoprotein • nitric oxide synthase mRNA • lysophosphatidylcholine
This article has been cited by other articles:
 |
|
 |
|
  C. D. Searles Transcriptional and posttranscriptional regulation of endothelial nitric oxide synthase expression Am J Physiol Cell Physiol, November 1, 2006; 291(5): C803 - C816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Fearon OxLDL enhances L-type Ca2+ currents via lysophosphatidylcholine-induced mitochondrial reactive oxygen species (ROS) production Cardiovasc Res, March 1, 2006; 69(4): 855 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Weber, C. H. Hagedorn, D. G. Harrison, and C. D. Searles Laminar Shear Stress and 3' Polyadenylation of eNOS mRNA Circ. Res., June 10, 2005; 96(11): 1161 - 1168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-U. Lee, I. K. Lee, J. Han, D.-K. Song, Y. M. Kim, H. S. Song, H. S. Kim, W. J. Lee, E. H. Koh, K.-H. Song, et al. Effects of Recombinant Adenovirus-Mediated Uncoupling Protein 2 Overexpression on Endothelial Function and Apoptosis Circ. Res., June 10, 2005; 96(11): 1200 - 1207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Searles, L. Ide, M. E. Davis, H. Cai, and M. Weber Actin Cytoskeleton Organization and Posttranscriptional Regulation of Endothelial Nitric Oxide Synthase During Cell Growth Circ. Res., September 3, 2004; 95(5): 488 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Tai, G. B. Robb, and P. A. Marsden Endothelial Nitric Oxide Synthase: A New Paradigm for Gene Regulation in the Injured Blood Vessel Arterioscler Thromb Vasc Biol, March 1, 2004; 24(3): 405 - 412. [Abstract] [Full Text]
|
|
|
|
|
|
C. R. W. Kuhlmann, M. Schafer, F. Li, T. Sawamura, H. Tillmanns, B. Waldecker, and J. Wiecha Modulation of endothelial Ca2+-activated K+ channels by oxidized LDL and its contribution to endothelial proliferation Cardiovasc Res, December 1, 2003; 60(3): 626 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Golodne, R. Q. Monteiro, A. V. Graca-Souza, M. A. C. Silva-Neto, and G. C. Atella Lysophosphatidylcholine Acts as an Anti-hemostatic Molecule in the Saliva of the Blood-sucking Bug Rhodnius prolixus J. Biol. Chem., July 18, 2003; 278(30): 27766 - 27771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Rikitake, K.-i. Hirata, T. Yamashita, K. Iwai, S. Kobayashi, H. Itoh, M. Ozaki, J. Ejiri, M. Shiomi, N. Inoue, et al. Expression of G2A, a Receptor for Lysophosphatidylcholine, by Macrophages in Murine, Rabbit, and Human Atherosclerotic Plaques Arterioscler Thromb Vasc Biol, December 1, 2002; 22(12): 2049 - 2053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Maas, E. Schwedhelm, J. Albsmeier, and R. H Boger The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function Vascular Medicine, August 1, 2002; 7(3): 213 - 225. [Abstract] [PDF]
|
|
|
|
 |
|
 J. L. Mehta and D. Li Identification, regulation and function of a novel lectin-like oxidized low-density lipoprotein receptor J. Am. Coll. Cardiol., May 1, 2002; 39(9): 1429 - 1435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Rikitake, S. Kawashima, T. Takahashi, T. Ueyama, S. Ishido, N. Inoue, K.-I. Hirata, and M. Yokoyama Regulation of tyrosine phosphorylation of PYK2 in vascular endothelial cells by lysophosphatidylcholine Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H266 - H274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Morimoto, N. Kume, S. Miyamoto, Y. Ueno, H. Kataoka, M. Minami, K. Hayashida, N. Hashimoto, and T. Kita Lysophosphatidylcholine Induces Early Growth Response Factor-1 Expression and Activates the Core Promoter of PDGF-A Chain in Vascular Endothelial Cells Arterioscler Thromb Vasc Biol, May 1, 2001; 21(5): 771 - 776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Govers and T. J. Rabelink Cellular regulation of endothelial nitric oxide synthase Am J Physiol Renal Physiol, February 1, 2001; 280(2): F193 - F206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Koh Effects of statins on vascular wall: vasomotor function, inflammation, and plaque stability Cardiovasc Res, September 1, 2000; 47(4): 648 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Colles and G. M. Chisolm Lysophosphatidylcholine-induced cellular injury in cultured fibroblasts involves oxidative events J. Lipid Res., August 1, 2000; 41(8): 1188 - 1198. [Abstract] [Full Text]
|
|
|
|
|
|
Q. Jing, S.-M. Xin, W.-B. Zhang, P. Wang, Y.-W. Qin, and G. Pei Lysophosphatidylcholine Activates p38 and p42/44 Mitogen-Activated Protein Kinases in Monocytic THP-1 Cells, but Only p38 Activation Is Involved in Its Stimulated Chemotaxis Circ. Res., July 7, 2000; 87(1): 52 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yokoyama, F. Shimizu, and M. Setaka Simultaneous separation of lysophospholipids from the total lipid fraction of crude biological samples using two-dimensional thin-layer chromatography J. Lipid Res., January 1, 2000; 41(1): 142 - 147. [Abstract] [Full Text]
|
|
|
|
|
|
A. Gomez-Munoz, J. S. Martens, and U. P. Steinbrecher Stimulation of Phospholipase D Activity by Oxidized LDL in Mouse Peritoneal Macrophages Arterioscler Thromb Vasc Biol, January 1, 2000; 20(1): 135 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Cieslik, C.-M. Lee, J.-L. Tang, and K. K. Wu Transcriptional Regulation of Endothelial Nitric-oxide Synthase by an Interaction between Casein Kinase 2 and Protein Phosphatase 2A J. Biol. Chem., December 3, 1999; 274(49): 34669 - 34675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Hubel Oxidative Stress in the Pathogenesis of Preeclampsia Experimental Biology and Medicine, December 1, 1999; 222(3): 222 - 235. [Abstract] [Full Text]
|
|
|
|
|
|
C. D. Searles, Y. Miwa, D. G. Harrison, and S. Ramasamy Posttranscriptional Regulation of Endothelial Nitric Oxide Synthase During Cell Growth Circ. Res., October 1, 1999; 85(7): 588 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Papapetropoulos, R. D. Rudic, and W. C Sessa Molecular control of nitric oxide synthases in the cardiovascular system Cardiovasc Res, August 15, 1999; 43(3): 509 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M Dart and J. P.F Chin-Dusting Lipids and the endothelium Cardiovasc Res, August 1, 1999; 43(2): 308 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ishida, K.-i. Hirata, T. Sakoda, S. Kawashima, H. Akita, and M. Yokoyama Identification of mRNA for 5-HT1 and 5-HT2 receptor subtypes in human coronary arteries Cardiovasc Res, January 1, 1999; 41(1): 267 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nishi, N. Kume, Y. Ueno, H. Ochi, H. Moriwaki, and T. Kita Lysophosphatidylcholine Enhances Cytokine-Induced Interferon Gamma Expression in Human T Lymphocytes Circ. Res., September 7, 1998; 83(5): 508 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kume, T. Murase, H. Moriwaki, T. Aoyama, T. Sawamura, T. Masaki, and T. Kita Inducible Expression of Lectin-like Oxidized LDL Receptor-1 in Vascular Endothelial Cells Circ. Res., August 10, 1998; 83(3): 322 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. O. Cannon III Role of nitric oxide in cardiovascular disease: focus on the endothelium Clin. Chem., August 1, 1998; 44(8): 1809 - 1819. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Förstermann, J.-p. Boissel, and H. Kleinert Expressional control of the `constitutive' isoforms of nitric oxide synthase (NOS I and NOS III) FASEB J, July 1, 1998; 12(10): 773 - 790. [Abstract] [Full Text]
|
|
|
|
|
|
K. Cieslik, A. Zembowicz, J.-L. Tang, and K. K. Wu Transcriptional Regulation of Endothelial Nitric-oxide Synthase by Lysophosphatidylcholine J. Biol. Chem., June 12, 1998; 273(24): 14885 - 14890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Wong, K. Tran, G. N. Pierce, A. C. Chan, K. O, and P. C. Choy Lysophosphatidylcholine Stimulates the Release of Arachidonic Acid in Human Endothelial Cells J. Biol. Chem., March 20, 1998; 273(12): 6830 - 6836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ramasamy, S. Parthasarathy, and D. G. Harrison Regulation of endothelial nitric oxide synthase gene expression by oxidized linoleic acid J. Lipid Res., February 1, 1998; 39(2): 268 - 276. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Zhao and R. L. Tackett Oxidized Low-Density Lipoprotein Inhibits Acetylcholine-Induced Vasorelaxation and Increases 5-HT-Induced Vasoconstriction in Isolated Human Saphenous Vein J. Pharmacol. Exp. Ther., February 1, 1998; 284(2): 637 - 643. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. M. FARACI and D. D. HEISTAD Regulation of the Cerebral Circulation: Role of Endothelium and Potassium Channels Physiol Rev, January 1, 1998; 78(1): 53 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Balligand and P. J. Cannon Nitric Oxide Synthases and Cardiac Muscle : Autocrine and Paracrine Influences Arterioscler Thromb Vasc Biol, October 1, 1997; 17(10): 1846 - 1858. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Miwa, K.-i. Hirata, S. Kawashima, H. Akita, and M. Yokoyama Lysophosphatidylcholine Inhibits Receptor-Mediated Ca2+ Mobilization in Intact Endothelial Cells of Rabbit Aorta Arterioscler Thromb Vasc Biol, August 1, 1997; 17(8): 1561 - 1567. [Abstract] [Full Text]
|
|
|
|
|
|
S. Hara, T. Shike, N. Takasu, and T. Mizui Lysophosphatidylcholine Promotes Cholesterol Efflux From Mouse Macrophage Foam Cells Arterioscler Thromb Vasc Biol, July 1, 1997; 17(7): 1258 - 1266. [Abstract] [Full Text]
|
|
|
|
|
|
X. Fang, S. Gibson, M. Flowers, T. Furui, R. C. Bast Jr., and G. B. Mills Lysophosphatidylcholine Stimulates Activator Protein 1 and the c-Jun N-terminal Kinase Activity J. Biol. Chem., May 23, 1997; 272(21): 13683 - 13689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Davidge, A. P. Signorella, C. A. Hubel, D. L. Lykins, and J. M. Roberts Distinct Factors in Plasma of Preeclamptic Women Increase Endothelial Nitric Oxide or Prostacyclin Hypertension, November 1, 1996; 28(5): 758 - 764. [Abstract] [Full Text]
|
|